Gene/Proteome Database (LMPD)

LMPD ID
LMP012787
Gene ID
83572
Species
Rattus norvegicus (Rat)
Gene Name
platelet-activating factor acetylhydrolase 1b, regulatory subunit 1
Gene Symbol
Pafah1b1
Synonyms
LIS1;
Alternate Names
platelet-activating factor acetylhydrolase IB subunit alpha; PAF-AH beta; LIS-1 {ECO:0000255|HAMAP-Rule:MF_03141}; PAFAH alpha {ECO:0000255|HAMAP-Rule:MF_03141}; PAF-AH alpha {ECO:0000255|HAMAP-Rule:MF_03141}; PAF-AH 45 kDa subunit {ECO:0000255|HAMAP-Rule:MF_03141}; lissencephaly-1 protein {ECO:0000255|HAMAP-Rule:MF_03141}; platelet-activating factor acetylhydrolase, isoform 1b, subunit 1; PAF acetylhydrolase 45 kDa subunit {ECO:0000255|HAMAP-Rule:MF_03141}; platelet-activating factor acetylhydrolase beta subunit (PAF-AH beta); platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa; platelet-activating factor acetylhydrolase IB subunit alpha {ECO:0000255|HAMAP-Rule:MF_03141};
Chromosome
10
Map Location
10q24
Summary
subunit of the brain intracellular platelet-activating factor acetylhydrolase, which is composed of alpha1, alpha2, and beta subunits [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Pafah1b1

Proteins

platelet-activating factor acetylhydrolase IB subunit alpha
Refseq ID NP_113951
Protein GI 13929078
UniProt ID P63004
mRNA ID NM_031763
Length 410
MVLSQRQRDELNRAIADYLRSNGYEEAYSVFKKEAELDMNEELDKKYAGLLEKKWTSVIRLQKKVMELESKLNEAKEEFTSGGPLGQKRDPKEWIPRPPEKYALSGHRSPVTRVIFHPVFSVMVSASEDATIKVWDYETGDFERTLKGHTDSVQDISFDHSGKLLASCSADMTIKLWDFQGFECIRTMHGHDHNVSSVAIMPNGDHIVSASRDKTIKMWEVQTGYCVKTFTGHREWVRMVRPNQDGTLIASCSNDQTVRVWVVATKECKAELREHEHVVECISWAPESSYSSISEATGSETKKSGKPGPFLLSGSRDKTIKMWDVSTGMCLMTLVGHDNWVRGVLFHSGGKFILSCADDKTLRVWDYKNKRCMKTLNAHEHFVTSLDFHKTAPYVVTGSVDQTVKVWECR

Gene Information

Entrez Gene ID
83572
Gene Name
platelet-activating factor acetylhydrolase 1b, regulatory subunit 1
Gene Symbol
Pafah1b1
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0000235 IEA:Ensembl C astral microtubule
GO:0030424 IDA:RGD C axon
GO:0005938 IEA:Ensembl C cell cortex
GO:0031252 IEA:Ensembl C cell leading edge
GO:0005813 IEA:Ensembl C centrosome
GO:0005829 IDA:RGD C cytosol
GO:0070062 IEA:Ensembl C extracellular vesicular exosome
GO:0030426 IDA:RGD C growth cone
GO:0005871 IDA:RGD C kinesin complex
GO:0000776 IEA:Ensembl C kinetochore
GO:0016020 IEA:UniProtKB-KW C membrane
GO:0031512 IEA:Ensembl C motile primary cilium
GO:0043005 IDA:RGD C neuron projection
GO:0043025 IDA:RGD C neuronal cell body
GO:0005635 IDA:MGI C nuclear envelope
GO:0048471 IEA:Ensembl C perinuclear region of cytoplasm
GO:0031982 IDA:RGD C vesicle
GO:0045505 IDA:RGD F dynein intermediate chain binding
GO:0047179 TAS:RGD F platelet-activating factor acetyltransferase activity
GO:0032403 IPI:RGD F protein complex binding
GO:0001675 IEA:Ensembl P acrosome assembly
GO:0030036 IEA:Ensembl P actin cytoskeleton organization
GO:0008344 IEA:Ensembl P adult locomotory behavior
GO:0001667 IEA:Ensembl P ameboidal cell migration
GO:0007420 IEP:RGD P brain development
GO:0048854 IEA:Ensembl P brain morphogenesis
GO:0021895 IMP:RGD P cerebral cortex neuron differentiation
GO:0021540 IEA:Ensembl P corpus callosum morphogenesis
GO:0051660 IMP:RGD P establishment of centrosome localization
GO:0000132 IEA:UniProtKB-HAMAP P establishment of mitotic spindle orientation
GO:0021766 IEA:Ensembl P hippocampus development
GO:0021819 IEA:Ensembl P layer formation in cerebral cortex
GO:0007611 IEA:Ensembl P learning or memory
GO:0016042 IEA:UniProtKB-KW P lipid catabolic process
GO:0031023 IEA:Ensembl P microtubule organizing center organization
GO:0007067 IEA:UniProtKB-KW P mitotic nuclear division
GO:0046329 IEA:Ensembl P negative regulation of JNK cascade
GO:0010977 IMP:RGD P negative regulation of neuron projection development
GO:0007405 IEA:Ensembl P neuroblast proliferation
GO:0050885 IEA:Ensembl P neuromuscular process controlling balance
GO:0001764 IMP:RGD P neuron migration
GO:0051081 IEA:Ensembl P nuclear envelope disassembly
GO:0007097 IMP:RGD P nuclear migration
GO:0036035 IEA:Ensembl P osteoclast development
GO:0045773 IMP:RGD P positive regulation of axon extension
GO:0001961 IEA:Ensembl P positive regulation of cytokine-mediated signaling pathway
GO:0045931 IMP:RGD P positive regulation of mitotic cell cycle
GO:0009306 IEA:Ensembl P protein secretion
GO:0032319 IEA:Ensembl P regulation of Rho GTPase activity
GO:0008090 IEA:Ensembl P retrograde axon cargo transport
GO:0017145 IMP:RGD P stem cell division
GO:0007268 IEA:Ensembl P synaptic transmission
GO:0019226 IEA:Ensembl P transmission of nerve impulse
GO:0047496 IEA:Ensembl P vesicle transport along microtubule

KEGG Pathway Links

KEGG Pathway ID Description
ko00565 Ether lipid metabolism
rno00565 Ether lipid metabolism
rno01100 Metabolic pathways

REACTOME Pathway Links

REACTOME Pathway ID Description
5953205 Cell Cycle
5953204 Cell Cycle, Mitotic
5954157 Centrosome maturation
5953690 G2/M Transition
5954155 Loss of Nlp from mitotic centrosomes
5954156 Loss of proteins required for interphase microtubule organization from the centrosome
5953688 M Phase
5953853 Mitotic Anaphase
5953691 Mitotic G2-G2/M phases
5953742 Mitotic Metaphase and Anaphase
5954139 Mitotic Prometaphase
5954159 Recruitment of mitotic centrosome proteins and complexes
5954588 Regulation of PLK1 Activity at G2/M Transition
5954495 Resolution of Sister Chromatid Cohesion
5953852 Separation of Sister Chromatids

Domain Information

InterPro Annotations

Accession Description
IPR017252 Dynein_regulator_LIS1
IPR020472 G-protein beta WD-40 repeat
IPR006594 LisH dimerisation motif
IPR013720 LisH dimerisation motif, subgroup
IPR019775 WD40 repeat, conserved site
IPR017986 WD40-repeat-containing domain
IPR015943 WD40/YVTN repeat-like-containing domain
IPR001680 WD40_repeat

UniProt Annotations

Entry Information

Gene Name
platelet-activating factor acetylhydrolase 1b, regulatory subunit 1
Protein Entry
LIS1_RAT
UniProt ID
P63004
Species
Rat

Comments

Comment Type Description
Domain Dimerization mediated by the LisH domain may be required to activate dynein
Function Required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet- activating factor (PAF) by removing the acetyl group at the SN-2 position (By similarity). Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing. {ECO:0000250, ECO:0000269|PubMed:11056532, ECO:0000269|PubMed:16144905}.
Similarity Belongs to the WD repeat LIS1/nudF family
Similarity Contains 1 LisH domain. {ECO:0000255|HAMAP- Rule:MF_03141}.
Similarity Contains 7 WD repeats. {ECO:0000255|HAMAP- Rule:MF_03141}.
Subcellular Location Cytoplasm, cytoskeleton {ECO:0000255|HAMAP- Rule:MF_03141}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Nucleus membrane . Note=Localizes to the plus end of microtubules and to the centrosome. May localize to the nuclear membrane. Also localizes to the microtubules of the manchette in elongating spermatids and to the meiotic spindle in spermatocytes (By similarity). Redistributes to axons during neuronal development. {ECO:0000255|HAMAP-Rule:MF_03141, ECO:0000269|PubMed:11001923, ECO:0000269|PubMed:11056530, ECO:0000269|PubMed:16481446}.
Subunit Can self-associate. Interacts with DCX, dynactin, IQGAP1, KATNB1, NDE1, NUDC and RSN. Interacts with DISC1, and this interaction is enhanced by NDEL1. Interacts with DAB1 when DAB1 is phosphorylated in response to RELN/reelin signaling. Component of cytosolic PAF-AH IB, which is composed of PAFAH1B1 (alpha), PAFAH1B2 (beta) and PAFAH1B3 (gamma) subunits. Trimer formation is not essential for the catalytic activity of the enzyme which is contributed solely by the PAFAH1B2 (beta) and PAFAH1B3 (gamma) subunits (By similarity). Interacts with dynein and NDEL1. Interacts with ASUN (By similarity)
Tissue Specificity Expressed in most tissues, with highest expression in brain

Identical and Related Proteins

Unique RefSeq proteins for LMP012787 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
13929078 RefSeq NP_113951 410 platelet-activating factor acetylhydrolase IB subunit alpha

Identical Sequences to LMP012787 proteins

Reference Database Accession Length Protein Name
GI:13929078 RefSeq XP_008994595.1 410 PREDICTED: platelet-activating factor acetylhydrolase IB subunit alpha [Callithrix jacchus]
GI:13929078 RefSeq XP_008959733.1 410 PREDICTED: platelet-activating factor acetylhydrolase IB subunit alpha [Pan paniscus]
GI:13929078 RefSeq XP_009429813.1 410 PREDICTED: platelet-activating factor acetylhydrolase IB subunit alpha isoform X1 [Pan troglodytes]
GI:13929078 RefSeq XP_010341907.1 410 PREDICTED: platelet-activating factor acetylhydrolase IB subunit alpha isoform X1 [Saimiri boliviensis boliviensis]

Related Sequences to LMP012787 proteins

Reference Database Accession Length Protein Name
GI:13929078 RefSeq XP_003799123.1 410 PREDICTED: platelet-activating factor acetylhydrolase IB subunit alpha [Otolemur garnettii]
GI:13929078 RefSeq XP_003809329.1 410 PREDICTED: platelet-activating factor acetylhydrolase IB subunit alpha [Pan paniscus]
GI:13929078 RefSeq XP_005891252.1 410 PREDICTED: platelet-activating factor acetylhydrolase IB subunit alpha isoform X1 [Bos mutus]