Gene/Proteome Database (LMPD)

LMPD ID
LMP012793
Gene ID
83709
Species
Rattus norvegicus (Rat)
Gene Name
G protein-coupled receptor kinase interacting ArfGAP 1
Gene Symbol
Git1
Alternate Names
ARF GTPase-activating protein GIT1; CAT1; CAT-1; ARF GAP GIT1; GRK-interacting protein 1; G protein-coupled receptor kinase interactor 1; G protein-coupled receptor kinase-interactor 1; cool-associated and tyrosine-phosphorylated protein 1; G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein (GIT1);
Chromosome
10
Map Location
10q26
Summary
mediates beta2-adrenergic receptor endocytosis [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Git1

Proteins

ARF GTPase-activating protein GIT1
Refseq ID NP_114002
Protein GI 13929158
UniProt ID Q9Z272
mRNA ID NM_031814
Length 770
MSRKGPRAEVCADCSAPDPGWASISRGVLVCDECCSVHRSLGRHISIVKHLRHSAWPPTLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKVHPIKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQTLQAELLVVYGADPGSPDVNGRTPIDYARQAGHHELAERLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADRSRQKCMSQSLDLSELAKAAKKKLQALSNRLFEELAMDVYDEVDRRENDAVWLATQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSEAKRRQQGKSLSSPTDNLELSARNQSDLDDQHDYDSVASDEDTDQEPLPSAGATRNNRARSMDSSDLSDGAVTLQEYLELKKALATSEAKVQQLMKVNSSLSDELRKLQREIHKLQAENLQLRQPPGPVPVPSLPSERAEHTLMGPGGSTHRRDRQAFSMYEPGSALKPFGGAPGDELATRLQPFHSTELEDDAIYSVHVPAGLYRIRKGVSASSVTFTPSSPLLSSSQEGSRHASKLSRHGSGAESDYENTQSGEPLLGLEGKRFLELSKEDELHAELESLDGDPDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPKRPALEPVRSSLRLLNASAYRLQSECRKTVPPEPGAPVDFQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Gene Information

Entrez Gene ID
83709
Gene Name
G protein-coupled receptor kinase interacting ArfGAP 1
Gene Symbol
Git1
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IEA:UniProtKB-KW C cytoplasm
GO:0005925 TAS:RGD C focal adhesion
GO:0008060 IDA:RGD F ARF GTPase activator activity
GO:0042803 IDA:RGD F protein homodimerization activity
GO:0031267 IDA:RGD F small GTPase binding
GO:0008270 IEA:InterPro F zinc ion binding
GO:0043547 IDA:GOC P positive regulation of GTPase activity
GO:0032312 IEA:InterPro P regulation of ARF GTPase activity
GO:0008277 IDA:RGD P regulation of G-protein coupled receptor protein signaling pathway

KEGG Pathway Links

KEGG Pathway ID Description
ko04144 Endocytosis
rno04144 Endocytosis
ko04810 Regulation of actin cytoskeleton
rno04810 Regulation of actin cytoskeleton

Domain Information

InterPro Annotations

Accession Description
IPR002110 Ankyrin repeat
IPR020683 Ankyrin repeat-containing domain
IPR001164 Arf GTPase activating protein
IPR022018 GIT1_C
IPR013724 GIT_SHD

UniProt Annotations

Entry Information

Gene Name
G protein-coupled receptor kinase interacting ArfGAP 1
Protein Entry
GIT1_RAT
UniProt ID
Q9Z272
Species
Rat

Comments

Comment Type Description
Function GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine- phosphorylated paxillin in cytoplasmic complexes.
Interaction Q9ES28:Arhgef7 (xeno); NbExp=2; IntAct=EBI-3842379, EBI-642580;
Ptm Phosphorylated on tyrosine residues by PTK2/FAK1 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase (By similarity)
Similarity Contains 1 Arf-GAP domain. {ECO:0000255|PROSITE- ProRule:PRU00288}.
Similarity Contains 3 ANK repeats. {ECO:0000255|PROSITE- ProRule:PRU00023}.
Subcellular Location Cytoplasm . Note=Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine- phosphorylated paxillin (By similarity)
Subunit Interacts with SCRIB (By similarity). Interacts with G protein-coupled receptor kinases: ADRBK1/GRK2, PPFIA1 and PPFIA4, with ARHGEF6/alpha-PIX, with ARHGEF7/beta-PIX, with PXN/paxillin and with PTK2/FAK1. Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and PAK1. Interacts with TGFB1I1. {ECO:0000250, ECO:0000269|PubMed:10938112, ECO:0000269|PubMed:12153727, ECO:0000269|PubMed:12629171, ECO:0000269|PubMed:9826657}.
Tissue Specificity Expressed in numerous tissues, with highest levels in testis

Identical and Related Proteins

Unique RefSeq proteins for LMP012793 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
13929158 RefSeq NP_114002 770 ARF GTPase-activating protein GIT1

Identical Sequences to LMP012793 proteins

Reference Database Accession Length Protein Name
GI:13929158 GenBank AAC83348.1 770 G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein [Rattus norvegicus]
GI:13929158 SwissProt Q9Z272.1 770 RecName: Full=ARF GTPase-activating protein GIT1; Short=ARF GAP GIT1; AltName: Full=Cool-associated and tyrosine-phosphorylated protein 1; Short=CAT-1; Short=CAT1; AltName: Full=G protein-coupled receptor kinase-interactor 1; AltName: Full=GRK-interacting protein 1 [Rattus norvegicus]

Related Sequences to LMP012793 proteins

Reference Database Accession Length Protein Name
GI:13929158 GenBank AAC83348.1 770 G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein [Rattus norvegicus]
GI:13929158 RefSeq NP_001004144.1 770 ARF GTPase-activating protein GIT1 [Mus musculus]
GI:13929158 SwissProt Q9Z272.1 770 RecName: Full=ARF GTPase-activating protein GIT1; Short=ARF GAP GIT1; AltName: Full=Cool-associated and tyrosine-phosphorylated protein 1; Short=CAT-1; Short=CAT1; AltName: Full=G protein-coupled receptor kinase-interactor 1; AltName: Full=GRK-interacting protein 1 [Rattus norvegicus]