Gene/Proteome Database (LMPD)

LMPD ID
LMP012799
Gene ID
83817
Species
Rattus norvegicus (Rat)
Gene Name
acetylcholinesterase
Gene Symbol
Ache
Alternate Names
acetylcholinesterase; glycolipid-anchored form of acetylcholinesterase;
Chromosome
12
Map Location
chromosome:12
EC Number
3.1.1.7
Summary
catalyzes the hydrolysis of acetylcholine to choline and acetate; plays a role in neuronal development and synapse formation [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Ache

Proteins

acetylcholinesterase precursor
Refseq ID NP_742006
Protein GI 25282401
UniProt ID P37136
mRNA ID NM_172009
Length 614
MRPPWYPLHTPSLASPLLFLLLSLLGGGARAEGREDPQLLVRVRGGQLRGIRLKAPGGPVSAFLGIPFAEPPVGSRRFMPPEPKRPWSGILDATTFQNVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPTSPTPVLIWIYGGGFYSGASSLDVYDGRFLAQVEGTVLVSMNYRVGTFGFLALPGSREAPGNVGLLDQRLALQWVQENIAAFGGDPMSVTLFGESAGAASVGMHILSLPSRSLFHRAVLQSGTPNGPWATVSAGEARRRATLLARLVGCPPGGAGGNDTELISCLRTRPAQDLVDHEWHVLPQESIFRFSFVPVVDGDFLSDTPDALINTGDFQDLQVLVGVVKDEGSYFLVYGVPGFSKDNESLISRAQFLAGVRIGVPQASDLAAEAVVLHYTDWLHPEDPAHLRDAMSAVVGDHNVVCPVAQLAGRLAAQGARVYAYIFEHRASTLTWPLWMGVPHGYEIEFIFGLPLDPSLNYTVEERIFAQRLMQYWTNFARTGDPNDPRDSKSPRWPPYTTAAQQYVSLNLKPLEVRRGLRAQTCAFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFDHYSKQERCSDL
sig_peptide: 1..31 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 3346 peptide sequence: MRPPWYPLHTPSLASPLLFLLLSLLGGGARA mat_peptide: 32..614 product: Acetylcholinesterase experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (P37136.1) calculated_mol_wt: 64869 peptide sequence: EGREDPQLLVRVRGGQLRGIRLKAPGGPVSAFLGIPFAEPPVGSRRFMPPEPKRPWSGILDATTFQNVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPTSPTPVLIWIYGGGFYSGASSLDVYDGRFLAQVEGTVLVSMNYRVGTFGFLALPGSREAPGNVGLLDQRLALQWVQENIAAFGGDPMSVTLFGESAGAASVGMHILSLPSRSLFHRAVLQSGTPNGPWATVSAGEARRRATLLARLVGCPPGGAGGNDTELISCLRTRPAQDLVDHEWHVLPQESIFRFSFVPVVDGDFLSDTPDALINTGDFQDLQVLVGVVKDEGSYFLVYGVPGFSKDNESLISRAQFLAGVRIGVPQASDLAAEAVVLHYTDWLHPEDPAHLRDAMSAVVGDHNVVCPVAQLAGRLAAQGARVYAYIFEHRASTLTWPLWMGVPHGYEIEFIFGLPLDPSLNYTVEERIFAQRLMQYWTNFARTGDPNDPRDSKSPRWPPYTTAAQQYVSLNLKPLEVRRGLRAQTCAFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFDHYSKQERCSDL

Gene Information

Entrez Gene ID
83817
Gene Name
acetylcholinesterase
Gene Symbol
Ache
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005794 IDA:RGD C Golgi apparatus
GO:0031225 IEA:UniProtKB-KW C anchored component of membrane
GO:0030424 IDA:RGD C axon
GO:0030054 IEA:UniProtKB-KW C cell junction
GO:0009986 IDA:RGD C cell surface
GO:0005737 IDA:RGD C cytoplasm
GO:0030425 IDA:RGD C dendrite
GO:0005788 IDA:RGD C endoplasmic reticulum lumen
GO:0005615 IBA:RefGenome C extracellular space
GO:0031594 IBA:RefGenome C neuromuscular junction
GO:0043005 IDA:RGD C neuron projection
GO:0043025 IDA:RGD C neuronal cell body
GO:0005635 IDA:RGD C nuclear envelope
GO:0048471 IDA:RGD C perinuclear region of cytoplasm
GO:0005886 IDA:RGD C plasma membrane
GO:0045211 IDA:RGD C postsynaptic membrane
GO:0042734 IDA:RGD C presynaptic membrane
GO:0005791 IDA:RGD C rough endoplasmic reticulum
GO:0045202 IDA:RGD C synapse
GO:0042166 IDA:RGD F acetylcholine binding
GO:0003990 IDA:RGD F acetylcholinesterase activity
GO:0033265 IDA:RGD F choline binding
GO:0004104 IBA:RefGenome F cholinesterase activity
GO:0006581 IDA:RGD P acetylcholine catabolic process
GO:0008291 IMP:RGD P acetylcholine metabolic process
GO:0019695 IDA:RGD P choline metabolic process
GO:0070997 IMP:RGD P neuron death
GO:0050772 IMP:RGD P positive regulation of axonogenesis
GO:0050775 IMP:RGD P positive regulation of dendrite morphogenesis
GO:0007416 IMP:RGD P synapse assembly
GO:0007271 IBA:RefGenome P synaptic transmission, cholinergic

KEGG Pathway Links

KEGG Pathway ID Description
rno04725 Cholinergic synapse
ko00564 Glycerophospholipid metabolism
rno00564 Glycerophospholipid metabolism

Domain Information

InterPro Annotations

Accession Description
IPR014788 Acetylcholinesterase, tetramerisation domain
IPR029058 Alpha/Beta hydrolase fold
IPR019826 Carboxylesterase type B, active site
IPR019819 Carboxylesterase type B, conserved site
IPR002018 Carboxylesterase, type B
IPR000997 Cholinesterase

UniProt Annotations

Entry Information

Gene Name
acetylcholinesterase
Protein Entry
ACES_RAT
UniProt ID
P37136
Species
Rat

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=3; Name=T; IsoId=P37136-1; Sequence=Displayed; Name=H; IsoId=P37136-2; Sequence=VSP_001458; Name=R; IsoId=P37136-3; Sequence=VSP_001459; Note=May be not functional.;
Catalytic Activity Acetylcholine + H(2)O = choline + acetate.
Function Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.
Similarity Belongs to the type-B carboxylesterase/lipase family
Subcellular Location Cell junction, synapse. Secreted. Cell membrane {ECO:0000250}; Peripheral membrane protein .
Subcellular Location Isoform H: Cell membrane; Lipid-anchor, GPI- anchor; Extracellular side.
Subunit Homotetramer; composed of disulfide-linked homodimers. Catalytic forms H (GPI-anchor dimer) and T (asymmetric collagen- tailed), which differ in their C-terminus, account for all types of known ACHE forms. Interacts with PRIMA1. The interaction with PRIMA1 is required to anchor it to the basal lamina of cells and organize into tetramers (By similarity)
Tissue Specificity Has been found in central nervous system and muscle. Found in embryonic liver and spleen but not in adult liver.

Identical and Related Proteins

Unique RefSeq proteins for LMP012799 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
25282401 RefSeq NP_742006 614 acetylcholinesterase precursor

Identical Sequences to LMP012799 proteins

Reference Database Accession Length Protein Name
GI:25282401 GenBank AAB24586.1 614 acetylcholinesterase T subunit [Rattus sp.]
GI:25282401 SwissProt P37136.1 614 RecName: Full=Acetylcholinesterase; Short=AChE; Flags: Precursor [Rattus norvegicus]

Related Sequences to LMP012799 proteins

Reference Database Accession Length Protein Name
GI:25282401 EMBL CAJ90604.1 614 unnamed protein product [Rattus rattus]
GI:25282401 GenBank AAB24586.1 614 acetylcholinesterase T subunit [Rattus sp.]
GI:25282401 SwissProt P37136.1 614 RecName: Full=Acetylcholinesterase; Short=AChE; Flags: Precursor [Rattus norvegicus]