Gene/Proteome Database (LMPD)

LMPD ID
LMP012804
Gene ID
84006
Species
Rattus norvegicus (Rat)
Gene Name
protein kinase C, iota
Gene Symbol
Prkci
Synonyms
Pkcl;
Alternate Names
protein kinase C iota type; nPKC-iota; aPKC-lambda/iota; protein kinase C, lambda; atypical protein kinase C-lambda/iota;
Chromosome
2
Map Location
2q24
EC Number
2.7.11.13
Summary
member of the atypical subgroup of PKCs that do not contain the calcium binding domain and possess only one cysteine-rich region; plays a critical role in controlling cell growth and cell survival; enhances Rab2-mediated vesicle budding [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Prkci

Proteins

protein kinase C iota type
Refseq ID NP_114448
Protein GI 198041666
UniProt ID F1M7Y5
mRNA ID NM_032059
Length 596
MPTQRDSSTMSHTVACGGGGDHSHQVRVKAYYRGDIMITHFEPSISFEGLCSEVRDMCSFDNEQPFTMKWIDEEGDPCTVSSQLELEEAFRLYELNKDSELLIHVFPCVPERPGMPCPGEDKSIYRRGARRWRKLYCANGHTFQAKRFNRRAHCAICTDRIWGLGRQGYKCINCKLLVHKKCHKLVTIECGRHSLPPEPMMPMDQSSMHPDHTQTVIPYNPSSHESLDQVGEEKEAMNTRESGKASSSLGLQDFDLLRVIGRGSYAKVLLVRLKKTDRIYAMKVVKKELVNDDEDIDWVQTEKHVFEQASNHPFLVGLHSCFQTESRLFFVIEYVNGGDLMFHMQRQRKLPEEHARFYSAEISLALNYLHERGIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIVGSSDNPDQNTEDYLFQVILEKQIRIPRSLSVKAASVLKSFLNKDPKERLGCHPQTGFADIQGHPFFRNVDWDMMEQKQVVPPFKPNISGEFGLDNFDSQFTNEPVQLTPDDDDIVRKIDQSEFEGFEYINPLLMSAEECV

Gene Information

Entrez Gene ID
84006
Gene Name
protein kinase C, iota
Gene Symbol
Prkci
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0000139 IDA:GOC C Golgi membrane
GO:0043220 IEA:Ensembl C Schmidt-Lanterman incisure
GO:0016324 IEA:Ensembl C apical plasma membrane
GO:0031252 IDA:RGD C cell leading edge
GO:0005829 ISS:UniProtKB C cytosol
GO:0005768 IEA:UniProtKB-KW C endosome
GO:0070062 IEA:Ensembl C extracellular vesicular exosome
GO:0045171 IEA:Ensembl C intercellular bridge
GO:0015630 IEA:Ensembl C microtubule cytoskeleton
GO:0005634 ISS:UniProtKB C nucleus
GO:0005886 IDA:RGD C plasma membrane
GO:0043234 IDA:RGD C protein complex
GO:0005524 IDA:RGD F ATP binding
GO:0005543 IEA:Ensembl F phospholipid binding
GO:0019904 IPI:RGD F protein domain specific binding
GO:0004697 IDA:RGD F protein kinase C activity
GO:0004672 ISS:UniProtKB F protein kinase activity
GO:0008270 IEA:InterPro F zinc ion binding
GO:0048194 IDA:RGD P Golgi vesicle budding
GO:0007015 IEA:Ensembl P actin filament organization
GO:0016477 IMP:RGD P cell migration
GO:0045216 IEA:Ensembl P cell-cell junction organization
GO:0034613 IMP:RGD P cellular protein localization
GO:0032869 IMP:RGD P cellular response to insulin stimulus
GO:0035089 IEA:Ensembl P establishment of apical/basal cell polarity
GO:0042462 IEA:Ensembl P eye photoreceptor cell development
GO:0035556 IEA:InterPro P intracellular signal transduction
GO:0034351 ISS:UniProtKB P negative regulation of glial cell apoptotic process
GO:0043524 ISS:UniProtKB P negative regulation of neuron apoptotic process
GO:0051092 ISS:UniProtKB P positive regulation of NF-kappaB transcription factor activity
GO:2000353 ISS:UniProtKB P positive regulation of endothelial cell apoptotic process
GO:0090004 IEA:Ensembl P positive regulation of establishment of protein localization to plasma membrane
GO:0060252 ISS:UniProtKB P positive regulation of glial cell proliferation
GO:0046326 IEA:Ensembl P positive regulation of glucose import
GO:0010976 ISS:UniProtKB P positive regulation of neuron projection development
GO:0006468 IDA:RGD P protein phosphorylation
GO:0070555 IMP:RGD P response to interleukin-1
GO:0043434 IDA:RGD P response to peptide hormone

KEGG Pathway Links

KEGG Pathway ID Description
ko04144 Endocytosis
rno04144 Endocytosis
ko04390 Hippo signaling pathway
rno04390 Hippo signaling pathway
ko04910 Insulin signaling pathway
rno04910 Insulin signaling pathway
rno04611 Platelet activation
ko04015 Rap1 signaling pathway
rno04015 Rap1 signaling pathway
ko04530 Tight junction
rno04530 Tight junction

REACTOME Pathway Links

REACTOME Pathway ID Description
5954243 Cell junction organization
5954063 Cell-Cell communication
5954242 Cell-cell junction organization
5953381 Signal Transduction
5953395 Signalling by NGF
5954249 Tight junction interactions
5953945 p75 NTR receptor-mediated signalling
5953946 p75NTR recruits signalling complexes
5953947 p75NTR signals via NF-kB

Domain Information

InterPro Annotations

Accession Description
IPR000961 AGC-kinase, C-terminal
IPR020454 Diacylglycerol/phorbol-ester binding
IPR000270 Phox/Bem1p
IPR012233 Protein kinase C
IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain
IPR000719 Protein kinase domain
IPR017441 Protein kinase, ATP binding site
IPR017892 Protein kinase, C-terminal
IPR011009 Protein kinase-like domain
IPR008271 Serine/threonine-protein kinase, active site
IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain

UniProt Annotations

Entry Information

Gene Name
protein kinase C, iota
Protein Entry
KPCI_RAT
UniProt ID
F1M7Y5
Species
Rat

Comments

Comment Type Description
Catalytic Activity ATP + a protein = ADP + a phosphoprotein.
Domain The C1 zinc finger does not bind diacylglycerol (DAG)
Domain The OPR domain mediates interaction with SQSTM1
Domain The pseudosubstrate motif resembles the sequence around sites phosphorylated on target proteins, except the presence of a non-phosphorylatable residue in place of Ser, it modulates activity by competing with substrates
Enzyme Regulation Atypical PKCs (PRKCI and PRKCZ) exhibit an elevated basal enzymatic activity (that may be due to the interaction with SMG1 or SQSTM1) and are not regulated by diacylglycerol, phosphatidylserine, phorbol esters or calcium ions. Two specific sites, Thr-412 (activation loop of the kinase domain) and Thr-564 (turn motif), need to be phosphorylated for its full activation. Might also be a target for novel lipid activators that are elevated during nutrient-stimulated insulin secretion (By similarity)
Function Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABL oncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-induced apoptosis. In cultured neurons, prevents amyloid beta protein-induced apoptosis by interrupting cell death process at a very early step. In glioblastoma cells, may function downstream of phosphatidylinositol 3-kinase (PI3K) and PDPK1 in the promotion of cell survival by phosphorylating and inhibiting the pro-apoptotic factor BAD. Can form a protein complex in non- small cell lung cancer (NSCLC) cells with PARD6A and ECT2 and regulate ECT2 oncogenic activity by phosphorylation, which in turn promotes transformed growth and invasion. In response to nerve growth factor (NGF), acts downstream of SRC to phosphorylate and activate IRAK1, allowing the subsequent activation of NF-kappa-B and neuronal cell survival. Functions in the organization of the apical domain in epithelial cells by phosphorylating EZR. This step is crucial for activation and normal distribution of EZR at the early stages of intestinal epithelial cell differentiation. Forms a protein complex with LLGL1 and PARD6B independently of PARD3 to regulate epithelial cell polarity. Plays a role in microtubule dynamics in the early secretory pathway through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). In human coronary artery endothelial cells (HCAEC), is activated by saturated fatty acids and mediates lipid-induced apoptosis. Downstream of PI3K is required for insulin-stimulated glucose transport. Activates RAB4A and promotes its association with KIF3A which is required for the insulin- induced SLC2A4/GLUT4 translocation in adipocytes. Is essential in early embryogenesis and development of differentiating photoreceptors by playing a role in the establishment of epithelial and neuronal polarity (By similarity)
Ptm Phosphorylation at Thr-412 in the activation loop is not mandatory for activation (By similarity). Upon neuronal growth factor (NGF) stimulation, phosphorylated by SRC at Tyr-265, Tyr- 280 and Tyr-334. Phosphorylation at Tyr-265 facilitates binding to KPNB1/importin-beta regulating entry of PRKCI into the nucleus. Phosphorylation on Tyr-334 is important for NF-kappa-B stimulation
Similarity Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily
Similarity Contains 1 AGC-kinase C-terminal domain
Similarity Contains 1 OPR domain
Similarity Contains 1 phorbol-ester/DAG-type zinc finger
Similarity Contains 1 protein kinase domain
Subcellular Location Cytoplasm . Membrane {ECO:0000250}. Endosome . Nucleus . Note=Transported into the endosome through interaction with SQSTM1/p62. After phosphorylation by SRC, transported into the nucleus through interaction with KPNB1. Colocalizes with CDK7 in the cytoplasm and nucleus. Transported to vesicular tubular clusters (VTCs) through interaction with RAB2A.
Subunit Forms a complex with SQSTM1 and MP2K5 (By similarity). Interacts directly with SQSTM1 (Probable). Interacts with IKBKB. Interacts with PARD6A, PARD6B and PARD6G. Part of a quaternary complex containing aPKC, PARD3, a PARD6 protein (PARD6A, PARD6B or PARD6G) and a GTPase protein (CDC42 or RAC1). Part of a complex with LLGL1 and PARD6B. Interacts with ADAP1/CENTA1. Interaction with SMG1, through the ZN-finger domain, activates the kinase activity. Interacts with CDK7. Forms a complex with RAB2A and GAPDH involved in recruitment onto the membrane of vesicular tubular clusters (VTCs). Interacts with ECT2 ('Thr-359' phosphorylated form) (By similarity)

Identical and Related Proteins

Unique RefSeq proteins for LMP012804 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
198041666 RefSeq NP_114448 596 protein kinase C iota type

Identical Sequences to LMP012804 proteins

Reference Database Accession Length Protein Name
GI:198041666 SwissProt F1M7Y5.1 596 RecName: Full=Protein kinase C iota type; AltName: Full=Atypical protein kinase C-lambda/iota; Short=aPKC-lambda/iota; AltName: Full=nPKC-iota [Rattus norvegicus]

Related Sequences to LMP012804 proteins

Reference Database Accession Length Protein Name
GI:198041666 DBBJ BAD90305.1 674 mKIAA4165 protein, partial [Mus musculus]
GI:198041666 SwissProt Q62074.3 595 RecName: Full=Protein kinase C iota type; AltName: Full=Atypical protein kinase C-lambda/iota; Short=aPKC-lambda/iota; AltName: Full=nPKC-iota [Mus musculus]
GI:198041666 SwissProt F1M7Y5.1 596 RecName: Full=Protein kinase C iota type; AltName: Full=Atypical protein kinase C-lambda/iota; Short=aPKC-lambda/iota; AltName: Full=nPKC-iota [Rattus norvegicus]