Gene/Proteome Database (LMPD)

LMPD ID
LMP012817
Gene ID
84497
Species
Rattus norvegicus (Rat)
Gene Name
diacylglycerol O-acyltransferase 1
Gene Symbol
Dgat1
Synonyms
ARAT; Dgat;
Alternate Names
diacylglycerol O-acyltransferase 1; diglyceride acyltransferase; diacylglycerol acyltransferase; retinol O-fatty-acyltransferase; acyl-CoA retinol O-fatty-acyltransferase; acyl-coenzyme A:diacylglycerol acyltransferase 1;
Chromosome
7
Map Location
7q34
EC Number
2.3.1.20
Summary
enzyme that catalyzes the final step of triglyceride synthesis; uses diacylglycerol and fatty acyl CoA as substrates [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Dgat1

Proteins

diacylglycerol O-acyltransferase 1
Refseq ID NP_445889
Protein GI 17865335
UniProt ID Q9ERM3
mRNA ID NM_053437
Length 498
MGDRGGAGSSRRRRTGSRVSVQGGSGPKVEEDEVREAAVSPDLGAGGDAPAPAPAPAHTRDKDRQTSVGDGHWELRCHRLQDSLFSSDSGFSNYRGILNWCVVMLILSNARLSLENLIKYGILVDPIQVVSLFLKDPYSWPAPCLIIASNIFIVATFQIEKRLSVGALTEQMGLLLHVVNLATIICFPAAVALLVESITPVGSLFALASYSIIFLKLSSYRDVNLWCRQRRVKAKAVSAGKKVSGAAAQNTVSYPDNLTYRDLYYFIFAPTLCYELNFPRSPRIRKRFLLRRVLEMLFFTQLQVGLIQQWMVPTIQNSMKPFKDMDYSRIIERLLKLAVPNHLIWLIFFYWLFHSCLNAVAELLQFGDREFYRDWWNAESVTYFWQNWNIPVHKWCIRHFYKPMLRLGSNKWMARTGVFWASAFFHEYLVSIPLRMFRLWAFTAMMAQVPLAWIVNRFFQGNYGNAAVWVTLIIGQPVAVLMYVHDYYVLNYDAPVGA

Gene Information

Entrez Gene ID
84497
Gene Name
diacylglycerol O-acyltransferase 1
Gene Symbol
Dgat1
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0043231 IDA:RGD C intracellular membrane-bounded organelle
GO:0004144 IDA:RGD F diacylglycerol O-acyltransferase activity
GO:0019992 IDA:RGD F diacylglycerol binding
GO:0005504 IDA:RGD F fatty acid binding
GO:0050252 IEA:UniProtKB-EC F retinol O-fatty-acyltransferase activity
GO:0046486 IDA:RGD P glycerolipid metabolic process
GO:0030073 IMP:RGD P insulin secretion
GO:0019432 IDA:RGD P triglyceride biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ko04975 Fat digestion and absorption
rno04975 Fat digestion and absorption
ko00561 Glycerolipid metabolism
rno00561 Glycerolipid metabolism
rno01100 Metabolic pathways
ko00830 Retinol metabolism
rno00830 Retinol metabolism
M00089 Triacylglycerol biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
5954466 Acyl chain remodeling of DAG and TAG
5953288 Fatty acid, triacylglycerol, and ketone body metabolism
5953473 Glycerophospholipid biosynthesis
5953250 Metabolism
5953289 Metabolism of lipids and lipoproteins
5953474 Phospholipid metabolism
5953464 Triglyceride Biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR027251 Diacylglycerol O-acyltransferase 1
IPR004299 Membrane bound O-acyl transferase, MBOAT
IPR014371 Sterol O-acyltransferase, ACAT/DAG/ARE types

UniProt Annotations

Entry Information

Gene Name
diacylglycerol O-acyltransferase 1
Protein Entry
DGAT1_RAT
UniProt ID
Q9ERM3
Species
Rat

Comments

Comment Type Description
Catalytic Activity Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.
Catalytic Activity Acyl-CoA + retinol = CoA + retinyl ester.
Function Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders. In liver, plays a role in esterifying exogenous fatty acids to glycerol (By similarity)
Pathway Lipid metabolism; glycerolipid metabolism.
Similarity Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily
Subcellular Location Endoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein .
Subunit Homodimer or homotetramer

Identical and Related Proteins

Unique RefSeq proteins for LMP012817 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
17865335 RefSeq NP_445889 498 diacylglycerol O-acyltransferase 1

Identical Sequences to LMP012817 proteins

Reference Database Accession Length Protein Name
GI:17865335 GenBank AAG10084.1 498 diacylglycerol acyltransferase [Rattus norvegicus]
GI:17865335 SwissProt Q9ERM3.1 498 RecName: Full=Diacylglycerol O-acyltransferase 1; AltName: Full=Acyl-CoA retinol O-fatty-acyltransferase; Short=ARAT; Short=Retinol O-fatty-acyltransferase; AltName: Full=Diglyceride acyltransferase [Rattus norvegicus]

Related Sequences to LMP012817 proteins

Reference Database Accession Length Protein Name
GI:17865335 GenBank AAG10084.1 498 diacylglycerol acyltransferase [Rattus norvegicus]
GI:17865335 GenBank ABH81552.1 500 Sequence 17 from patent US 7041872
GI:17865335 SwissProt Q9ERM3.1 498 RecName: Full=Diacylglycerol O-acyltransferase 1; AltName: Full=Acyl-CoA retinol O-fatty-acyltransferase; Short=ARAT; Short=Retinol O-fatty-acyltransferase; AltName: Full=Diglyceride acyltransferase [Rattus norvegicus]