Gene/Proteome Database (LMPD)

LMPD ID
LMP012825
Gene ID
85255
Species
Rattus norvegicus (Rat)
Gene Name
2-hydroxyacyl-CoA lyase 1
Gene Symbol
Hacl1
Synonyms
Hpcl2; Phyh2;
Alternate Names
2-hydroxyacyl-CoA lyase 1; 2-HPCL; 2-hydroxyphytanoyl-CoA lyase; phytanoyl-CoA 2-hydroxylase 2; 2-hydroxyphytanoyl-Coenzyme A lyase; phytanoyl-Coenzyme A 2-hydroxylase 2;
Chromosome
16
Map Location
16p16
EC Number
4.1.-.-
Summary
human homolog catalyzes alpha-oxidation and carbon-carbon cleavage of 3-methyl-branched fatty acids [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Hacl1

Proteins

2-hydroxyacyl-CoA lyase 1
Refseq ID NP_445945
Protein GI 27436863
UniProt ID Q8CHM7
mRNA ID NM_053493
Length 581
MSESNSAEGSDRSEEQVSGAKVIAQALKTQDVEYMFGVVGIPVTEIALAAQELGIKYIGMRNEQAACYAASAVGYLTGRPGVCLVVSGPGLIHALGGMANANMNCWPLIVIGGSSERNQEAMGAFQEFPQVEACRLYSKFSARPSSIQHIPFVIEKAVRSSIYGRPGACYIDVPADLVTLQESTTSIKYKECCMPPPLSLAETSAVRAAASVLRSAKQPLLIIGKGAAYSHAEDSIRKLVELCNLPFLPTPMGKGVVPDNHPNCVGAARSRALQFADVIVLFGARLNWILHFGLPPRYQADVKFIQIDICAEELGNNVRPSVTLLGDVNAVSKQLLEQFVKNPWQYPTDSKWWETLREKRKNNEAVSKELASKKSLPMNYYTVFYHVQEQLPRNCFIVSEGANTMDIGRTVLQNYLPRHRLDAGSFGTMGVGLGFAIAAAVVAKERSPGQRVICVEGDSAFGFSGMEVETICRYNLPIIILVVNNNGIYQGFDADTWGKILNFQGTATTIPPMCLLPNSHYEQVMTAFGGKGYFVQTPEELQDSLRQALKDTSKPCLINIMIEPQSTRKAQDFHWLTRSNI

Gene Information

Entrez Gene ID
85255
Gene Name
2-hydroxyacyl-CoA lyase 1
Gene Symbol
Hacl1
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005777 IDA:HGNC C peroxisome
GO:0016830 IDA:HGNC F carbon-carbon lyase activity
GO:0000287 IEA:InterPro F magnesium ion binding
GO:0030976 IDA:HGNC F thiamine pyrophosphate binding
GO:0001561 IDA:HGNC P fatty acid alpha-oxidation
GO:0051259 IEA:Ensembl P protein oligomerization

KEGG Pathway Links

KEGG Pathway ID Description
ko04146 Peroxisome
rno04146 Peroxisome

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY66-387 fatty acid alpha-oxidation II

REACTOME Pathway Links

REACTOME Pathway ID Description
5954179 Alpha-oxidation of phytanate
5953250 Metabolism
5953289 Metabolism of lipids and lipoproteins
5953470 Peroxisomal lipid metabolism

Domain Information

InterPro Annotations

Accession Description
IPR029035 DHS-like NAD/FAD-binding domain
IPR029061 Thiamin diphosphate-binding fold
IPR011766 Thiamine pyrophosphate enzyme, C-terminal TPP-binding
IPR012001 Thiamine pyrophosphate enzyme, N-terminal TPP-binding domain
IPR012000 Thiamine pyrophosphate enzyme, central domain

UniProt Annotations

Entry Information

Gene Name
2-hydroxyacyl-CoA lyase 1
Protein Entry
HACL1_RAT
UniProt ID
Q8CHM7
Species
Rat

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=15 uM for 2-hydroxy-3-methylhexadecanoyl-CoA ; pH dependence: Optimum pH is 7.5-8. ;
Catalytic Activity 2-hydroxyphytanoyl-CoA = pristanal + formyl- CoA.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence= ; Note=Binds 1 Mg(2+) ion per subunit. ;
Cofactor Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937; Evidence= ; Note=Binds 1 thiamine pyrophosphate per subunit. ;
Function Catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde.
Pathway Lipid metabolism; fatty acid metabolism.
Similarity Belongs to the TPP enzyme family
Subcellular Location Peroxisome .
Subunit Homotetramer

Identical and Related Proteins

Unique RefSeq proteins for LMP012825 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
27436863 RefSeq NP_445945 581 2-hydroxyacyl-CoA lyase 1

Identical Sequences to LMP012825 proteins

Reference Database Accession Length Protein Name
GI:27436863 EMBL CAD56981.1 581 2-hydroxyphytanoyl-CoA lyase [Rattus norvegicus]
GI:27436863 GenBank AAH78697.1 581 2-hydroxyacyl-CoA lyase 1 [Rattus norvegicus]
GI:27436863 GenBank EDL88941.1 581 phytanoyl-CoA 2-hydroxylase 2 [Rattus norvegicus]
GI:27436863 SwissProt Q8CHM7.1 581 RecName: Full=2-hydroxyacyl-CoA lyase 1; AltName: Full=2-hydroxyphytanoyl-CoA lyase; Short=2-HPCL; AltName: Full=Phytanoyl-CoA 2-hydroxylase 2 [Rattus norvegicus]

Related Sequences to LMP012825 proteins

Reference Database Accession Length Protein Name
GI:27436863 EMBL CAD56981.1 581 2-hydroxyphytanoyl-CoA lyase [Rattus norvegicus]
GI:27436863 GenBank EDL88941.1 581 phytanoyl-CoA 2-hydroxylase 2 [Rattus norvegicus]
GI:27436863 SwissProt Q8CHM7.1 581 RecName: Full=2-hydroxyacyl-CoA lyase 1; AltName: Full=2-hydroxyphytanoyl-CoA lyase; Short=2-HPCL; AltName: Full=Phytanoyl-CoA 2-hydroxylase 2 [Rattus norvegicus]