LIPID MAPS

LMPD ID

LMP012825

Gene ID

85255

Species

Rattus norvegicus (Rat)

Gene Name

2-hydroxyacyl-CoA lyase 1

Gene Symbol

Hacl1

Synonyms

Hpcl2; Phyh2;

Alternate Names

2-hydroxyacyl-CoA lyase 1; 2-HPCL; 2-hydroxyphytanoyl-CoA lyase; phytanoyl-CoA 2-hydroxylase 2; 2-hydroxyphytanoyl-Coenzyme A lyase; phytanoyl-Coenzyme A 2-hydroxylase 2;

Chromosome

16

Map Location

16p16

EC Number

4.1.-.-

Summary

human homolog catalyzes alpha-oxidation and carbon-carbon cleavage of 3-methyl-branched fatty acids [RGD, Feb 2006]

Orthologs

View orthologs and multiple alignments for Hacl1

2-hydroxyacyl-CoA lyase 1
Refseq ID	NP_445945
Protein GI	27436863
UniProt ID	Q8CHM7
mRNA ID	NM_053493
Length	581
MSESNSAEGSDRSEEQVSGAKVIAQALKTQDVEYMFGVVGIPVTEIALAAQELGIKYIGMRNEQAACYAASAVGYLTGRPGVCLVVSGPGLIHALGGMANANMNCWPLIVIGGSSERNQEAMGAFQEFPQVEACRLYSKFSARPSSIQHIPFVIEKAVRSSIYGRPGACYIDVPADLVTLQESTTSIKYKECCMPPPLSLAETSAVRAAASVLRSAKQPLLIIGKGAAYSHAEDSIRKLVELCNLPFLPTPMGKGVVPDNHPNCVGAARSRALQFADVIVLFGARLNWILHFGLPPRYQADVKFIQIDICAEELGNNVRPSVTLLGDVNAVSKQLLEQFVKNPWQYPTDSKWWETLREKRKNNEAVSKELASKKSLPMNYYTVFYHVQEQLPRNCFIVSEGANTMDIGRTVLQNYLPRHRLDAGSFGTMGVGLGFAIAAAVVAKERSPGQRVICVEGDSAFGFSGMEVETICRYNLPIIILVVNNNGIYQGFDADTWGKILNFQGTATTIPPMCLLPNSHYEQVMTAFGGKGYFVQTPEELQDSLRQALKDTSKPCLINIMIEPQSTRKAQDFHWLTRSNI

Entrez Gene ID

85255

Gene Name

2-hydroxyacyl-CoA lyase 1

Gene Symbol

Hacl1

Species

Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005777	IDA:HGNC	C	peroxisome
GO:0016830	IDA:HGNC	F	carbon-carbon lyase activity
GO:0000287	IEA:InterPro	F	magnesium ion binding
GO:0030976	IDA:HGNC	F	thiamine pyrophosphate binding
GO:0001561	IDA:HGNC	P	fatty acid alpha-oxidation
GO:0051259	IEA:Ensembl	P	protein oligomerization

KEGG Pathway Links

KEGG Pathway ID	Description
ko04146	Peroxisome
rno04146	Peroxisome

BIOCYC Pathway Links

BIOCYC Pathway ID	Description
PWY66-387	fatty acid alpha-oxidation II

REACTOME Pathway Links

REACTOME Pathway ID	Description
5954179	Alpha-oxidation of phytanate
5953250	Metabolism
5953289	Metabolism of lipids and lipoproteins
5953470	Peroxisomal lipid metabolism

InterPro Annotations

Accession	Description
IPR029035	DHS-like NAD/FAD-binding domain
IPR029061	Thiamin diphosphate-binding fold
IPR011766	Thiamine pyrophosphate enzyme, C-terminal TPP-binding
IPR012001	Thiamine pyrophosphate enzyme, N-terminal TPP-binding domain
IPR012000	Thiamine pyrophosphate enzyme, central domain

Entry Information

Gene Name

2-hydroxyacyl-CoA lyase 1

Protein Entry

HACL1_RAT

UniProt ID

Q8CHM7

Species

Rat

Comments

Comment Type	Description
Biophysicochemical Properties	Kinetic parameters: KM=15 uM for 2-hydroxy-3-methylhexadecanoyl-CoA ; pH dependence: Optimum pH is 7.5-8. ;
Catalytic Activity	2-hydroxyphytanoyl-CoA = pristanal + formyl- CoA.
Cofactor	Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence= ; Note=Binds 1 Mg(2+) ion per subunit. ;
Cofactor	Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937; Evidence= ; Note=Binds 1 thiamine pyrophosphate per subunit. ;
Function	Catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde.
Pathway	Lipid metabolism; fatty acid metabolism.
Similarity	Belongs to the TPP enzyme family
Subcellular Location	Peroxisome .
Subunit	Homotetramer

Unique RefSeq proteins for LMP012825 (as displayed in Record Overview)

Protein GI	Database	Accession	Length	Protein Name
27436863	RefSeq	NP_445945	581	2-hydroxyacyl-CoA lyase 1

Identical Sequences to LMP012825 proteins

Reference	Database	Accession	Length	Protein Name
GI:27436863	EMBL	CAD56981.1	581	2-hydroxyphytanoyl-CoA lyase [Rattus norvegicus]
GI:27436863	GenBank	AAH78697.1	581	2-hydroxyacyl-CoA lyase 1 [Rattus norvegicus]
GI:27436863	GenBank	EDL88941.1	581	phytanoyl-CoA 2-hydroxylase 2 [Rattus norvegicus]
GI:27436863	SwissProt	Q8CHM7.1	581	RecName: Full=2-hydroxyacyl-CoA lyase 1; AltName: Full=2-hydroxyphytanoyl-CoA lyase; Short=2-HPCL; AltName: Full=Phytanoyl-CoA 2-hydroxylase 2 [Rattus norvegicus]

Related Sequences to LMP012825 proteins

Reference	Database	Accession	Length	Protein Name
GI:27436863	EMBL	CAD56981.1	581	2-hydroxyphytanoyl-CoA lyase [Rattus norvegicus]
GI:27436863	GenBank	EDL88941.1	581	phytanoyl-CoA 2-hydroxylase 2 [Rattus norvegicus]
GI:27436863	SwissProt	Q8CHM7.1	581	RecName: Full=2-hydroxyacyl-CoA lyase 1; AltName: Full=2-hydroxyphytanoyl-CoA lyase; Short=2-HPCL; AltName: Full=Phytanoyl-CoA 2-hydroxylase 2 [Rattus norvegicus]