Gene/Proteome Database (LMPD)

LMPD ID
LMP012832
Gene ID
89813
Species
Rattus norvegicus (Rat)
Gene Name
pyruvate dehydrogenase kinase, isozyme 4
Gene Symbol
Pdk4
Alternate Names
pyruvate dehydrogenase kinase, isozyme 4; pyruvate dehydrogenate kinase 4; pyruvate dehydrogenase kinase, isoenzyme 4; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial;
Chromosome
4
Map Location
4q13
EC Number
2.7.11.2
Summary
mediates glucose oxidation through inhibitory phosphorylation of the pyruvate dehydrogenase complex (PDC) [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Pdk4

Proteins

pyruvate dehydrogenase kinase, isozyme 4
Refseq ID NP_446003
Protein GI 16758318
UniProt ID O54937
mRNA ID NM_053551
Length 412
MKAARFVMRSASSLGNAGLVPREVELFSRYSPSPLSMKQLLDFGSENACERTSFSFLRQELPVRLANILKEIDILPEHLVNTPSVQLVKSWYIQSLMDLVEFHEKSPEDQKVLSDFVDTLVKVRNRHHNVVPTMAQGILEYKDNCTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIFSDSKTGNPSHIGSIDPNCDVVAVVEDAFECAKMLCDQYYLTSPELKLTQVNGKFPGQPIHIVYVPSHLHHMLFELFKNAMRATVEHQENRPFLTPVEATVVLGKEDLTIKISDRGGGVPLRITDRLFSYTYSTAPTPVMDNSRNAPLAGFGYGLPISRLYAKYFQGDLNLYSMSGYGTDAIIYLKALSSESIEKLPVFNKSAFKHYQMSSEADDWCIPSKEPKNLSKEKLAV

Gene Information

Entrez Gene ID
89813
Gene Name
pyruvate dehydrogenase kinase, isozyme 4
Gene Symbol
Pdk4
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005739 IDA:RGD C mitochondrion
GO:0005524 IDA:RGD F ATP binding
GO:0004674 IEA:UniProtKB-KW F protein serine/threonine kinase activity
GO:0004740 IDA:UniProtKB F pyruvate dehydrogenase (acetyl-transferring) kinase activity
GO:0006086 TAS:RGD P acetyl-CoA biosynthetic process from pyruvate
GO:0071398 ISS:UniProtKB P cellular response to fatty acid
GO:0009267 ISS:UniProtKB P cellular response to starvation
GO:0042593 ISS:UniProtKB P glucose homeostasis
GO:0006006 IEA:UniProtKB-KW P glucose metabolic process
GO:0008286 ISS:UniProtKB P insulin receptor signaling pathway
GO:0006468 IDA:RGD P protein phosphorylation
GO:0072593 ISS:UniProtKB P reactive oxygen species metabolic process
GO:0010510 ISS:UniProtKB P regulation of acetyl-CoA biosynthetic process from pyruvate
GO:0010565 ISS:UniProtKB P regulation of cellular ketone metabolic process
GO:0042304 ISS:UniProtKB P regulation of fatty acid biosynthetic process
GO:0046320 ISS:UniProtKB P regulation of fatty acid oxidation
GO:0010906 ISS:UniProtKB P regulation of glucose metabolic process
GO:0006885 ISS:UniProtKB P regulation of pH
GO:0042594 ISS:UniProtKB P response to starvation

Domain Information

InterPro Annotations

Accession Description
IPR018955 Branched-chain alpha-ketoacid dehydrogenase kinase/Pyruvate dehydrogenase kinase, N-terminal
IPR003594 Histidine kinase-like ATPase, C-terminal domain
IPR005467 Signal transduction histidine kinase, core

UniProt Annotations

Entry Information

Gene Name
pyruvate dehydrogenase kinase, isozyme 4
Protein Entry
PDK4_RAT
UniProt ID
O54937
Species
Rat

Comments

Comment Type Description
Catalytic Activity ATP + [pyruvate dehydrogenase (acetyl- transferring)] = ADP + [pyruvate dehydrogenase (acetyl- transferring)] phosphate. {ECO:0000269|PubMed:11486000, ECO:0000269|PubMed:9405293}.
Function Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism
Induction Up-regulated by exercise, starvation, glucocorticoids, thyroid hormone and epinephrine. Down-regulated by insulin. {ECO:0000269|PubMed:19703515, ECO:0000269|PubMed:19948729, ECO:0000269|PubMed:20739620}.
Similarity Belongs to the PDK/BCKDK protein kinase family
Similarity Contains 1 histidine kinase domain
Subcellular Location Mitochondrion matrix.
Subunit Homodimer. Interacts with the pyruvate dehydrogenase complex subunit DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3) (By similarity)
Tissue Specificity Ubiquitous; highest levels of expression in heart and skeletal muscle

Identical and Related Proteins

Unique RefSeq proteins for LMP012832 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16758318 RefSeq NP_446003 412 pyruvate dehydrogenase kinase, isozyme 4

Identical Sequences to LMP012832 proteins

Reference Database Accession Length Protein Name
GI:16758318 GenBank AAC00177.1 412 pyruvate dehydrogenase kinase isoenzyme 4 [Rattus norvegicus]
GI:16758318 SwissProt O54937.1 412 RecName: Full=[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial; AltName: Full=Pyruvate dehydrogenase kinase isoform 4; Flags: Precursor [Rattus norvegicus]

Related Sequences to LMP012832 proteins

Reference Database Accession Length Protein Name
GI:16758318 GenBank AAC00177.1 412 pyruvate dehydrogenase kinase isoenzyme 4 [Rattus norvegicus]
GI:16758318 GenBank EDM15025.1 412 pyruvate dehydrogenase kinase, isoenzyme 4 [Rattus norvegicus]
GI:16758318 SwissProt O54937.1 412 RecName: Full=[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial; AltName: Full=Pyruvate dehydrogenase kinase isoform 4; Flags: Precursor [Rattus norvegicus]