Gene/Proteome Database (LMPD)

LMPD ID
LMP012833
Gene ID
89816
Species
Rattus norvegicus (Rat)
Gene Name
phosphatidylinositol binding clathrin assembly protein
Gene Symbol
Picalm
Synonyms
Calm;
Alternate Names
phosphatidylinositol-binding clathrin assembly protein; rCALM; clathrin-assembly lymphoid leukemia protein; clathrin assembly lymphoid myeloid leukemia protein; clathrin-assembly lymphoid myeloid leukemia protein;
Chromosome
1
Map Location
1q32
Summary
may mediate the assembly of clathrin in neural and supporting cells of brain; may regulate the clathrin coated-vesicle formation [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Picalm

Proteins

phosphatidylinositol-binding clathrin assembly protein
Refseq ID NP_446006
Protein GI 16758324
UniProt ID O55012
mRNA ID NM_053554
Length 640
MSGQSLTDRITAAQHSVTGSAVSKTVCKATTHEIMGPKKKHLDYLIQCTNEMNVNIPQLADSLFERTTNSSWVVVFKSLITTHHLMVYGNERFIQYLASRNTLFNLSNFLDKSGLQGYDMSTFIRRYSRYLNEKAVSYRQVAFDFTKVKRGADGVMRTMNTEKLLKTVPIIQNQMDALLDFNVNSNELTNGVINAAFMLLFKDAIRLFAAYNEGIINLLEKYFDMKKNQCKEGLDIYKKFLTRMTRISEFLKVAEQVGIDRGDIPDLSQAPSSLLDALEQHLASLEGKKIKDSTAASRATTLSNAVSSLASTGLSLTKVDEREKQAALEEEQARLKALKEQRLKELAKKPHTSLTTAASPVSTSAGGIMTAPAIDIFSTPSSSNSTSKLPNDLLDLQQPTFHPSVHAMSAAPQVASTWGDAVDDAIPSLNPFLTKSSGDVHLPISSDVSTFTTRTPTHEMFVGFSPSPVTQPHPSAGLNVDFESVFGNKSTNVAVDSGGGLLKPTVASQNQSLPVAKLPPNKLVSDDLDSSLANLVGNLGIGNGTTKNDVSCSQPGEKKLTGGSNWQPKVAPTTAWSAATMAPPVMAYPATTPTGMIGYGIPPQMGSVPVMTQPTLIYSQPVMRPPNPFGPVPGAQIQFM

Gene Information

Entrez Gene ID
89816
Gene Name
phosphatidylinositol binding clathrin assembly protein
Gene Symbol
Picalm
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005794 IEA:UniProtKB-KW C Golgi apparatus
GO:0030118 IEA:InterPro C clathrin coat
GO:0030136 IDA:BHF-UCL C clathrin-coated vesicle
GO:0005905 ISS:UniProtKB C coated pit
GO:0005768 IDA:BHF-UCL C endosome
GO:0097418 ISS:Alzheimers_University_of_Toronto C neurofibrillary tangle
GO:0043025 ISS:Alzheimers_University_of_Toronto C neuronal cell body
GO:0097481 IDA:BHF-UCL C neuronal postsynaptic density
GO:0005634 IEA:UniProtKB-KW C nucleus
GO:0005886 IDA:BHF-UCL C plasma membrane
GO:0045211 IDA:BHF-UCL C postsynaptic membrane
GO:0042734 IDA:BHF-UCL C presynaptic membrane
GO:0045202 IDA:RGD C synapse
GO:0008021 IDA:BHF-UCL C synaptic vesicle
GO:0031982 ISS:Alzheimers_University_of_Toronto C vesicle
GO:0005545 IDA:UniProtKB F 1-phosphatidylinositol binding
GO:0017124 IDA:RGD F SH3 domain binding
GO:0035615 ISS:Alzheimers_University_of_Toronto F clathrin adaptor activity
GO:0030276 IDA:UniProtKB F clathrin binding
GO:0042802 IPI:IntAct F identical protein binding
GO:0007409 IMP:BHF-UCL P axonogenesis
GO:0035459 ISS:Alzheimers_University_of_Toronto P cargo loading into vesicle
GO:0008283 ISS:Alzheimers_University_of_Toronto P cell proliferation
GO:0048268 IDA:RGD P clathrin coat assembly
GO:0072583 ISS:Alzheimers_University_of_Toronto P clathrin-mediated endocytosis
GO:0048813 IMP:BHF-UCL P dendrite morphogenesis
GO:0055072 ISS:Alzheimers_University_of_Toronto P iron ion homeostasis
GO:0097459 ISS:Alzheimers_University_of_Toronto P iron ion import into cell
GO:0010629 ISS:Alzheimers_University_of_Toronto P negative regulation of gene expression
GO:1902963 ISS:Alzheimers_University_of_Toronto P negative regulation of metalloendopeptidase activity involved in amyloid precursor protein catabolic process
GO:1902961 ISS:Alzheimers_University_of_Toronto P positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process
GO:1902004 ISS:Alzheimers_University_of_Toronto P positive regulation of beta-amyloid formation
GO:1901216 ISS:Alzheimers_University_of_Toronto P positive regulation of neuron death
GO:0006898 ISS:UniProtKB P receptor-mediated endocytosis
GO:1902959 ISS:Alzheimers_University_of_Toronto P regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process
GO:0030100 IMP:BHF-UCL P regulation of endocytosis
GO:0051223 IMP:BHF-UCL P regulation of protein transport
GO:0016188 ISS:Alzheimers_University_of_Toronto P synaptic vesicle maturation
GO:0016192 IMP:BHF-UCL P vesicle-mediated transport

Domain Information

InterPro Annotations

Accession Description
IPR011417 AP180 N-terminal homology (ANTH) domain
IPR008942 ENTH_VHS
IPR013809 Epsin-like_N
IPR014712 Phosphoinositide-binding clathrin adaptor, domain 2

UniProt Annotations

Entry Information

Gene Name
phosphatidylinositol binding clathrin assembly protein
Protein Entry
PICAL_RAT
UniProt ID
O55012
Species
Rat

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1 ; Synonyms=Long ; IsoId=O55012-1; Sequence=Displayed; Name=2 ; Synonyms=Short ; IsoId=O55012-2; Sequence=VSP_050687;
Function Assembly protein recruiting clathrin and adapter protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction (By similarity)
Interaction Self; NbExp=3; IntAct=EBI-915601, EBI-915601; P63027:VAMP2 (xeno); NbExp=2; IntAct=EBI-915601, EBI-520113; Q15836:VAMP3 (xeno); NbExp=2; IntAct=EBI-915601, EBI-722343; O70404:Vamp8 (xeno); NbExp=4; IntAct=EBI-915601, EBI-1812572;
Similarity Belongs to the PICALM/SNAP91 family
Similarity Contains 1 ENTH (epsin N-terminal homology) domain
Subcellular Location Membrane, clathrin-coated pit . Golgi apparatus . Cytoplasmic vesicle, clathrin- coated vesicle {ECO:0000250}. Nucleus . Note=Colocalized with clathrin in the Golgi area. Interaction with FAM64A may target PICALM to the nucleus in some cells
Subunit Binds clathrin and phosphatidylinositol 4,5-bisphosphate. Interacts with FAM64A; this interaction may change the subcellular location into the nucleus (By similarity)
Tissue Specificity Isoform 2 was found in most tissues examined. Isoform 1 has an overlapping expression pattern but is absent from lung, heart and pancreas. Both isoforms are widely expressed in the brain, higher levels are seen in hippocampus, dentate gyrus, medial habenula nucleus and cerebellar granule cells

Identical and Related Proteins

Unique RefSeq proteins for LMP012833 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16758324 RefSeq NP_446006 640 phosphatidylinositol-binding clathrin assembly protein

Identical Sequences to LMP012833 proteins

Reference Database Accession Length Protein Name
GI:16758324 GenBank AAB97079.1 640 clathrin assembly protein long form [Rattus norvegicus]
GI:16758324 SwissProt O55012.1 640 RecName: Full=Phosphatidylinositol-binding clathrin assembly protein; AltName: Full=Clathrin assembly lymphoid myeloid leukemia protein; Short=rCALM [Rattus norvegicus]

Related Sequences to LMP012833 proteins

Reference Database Accession Length Protein Name
GI:16758324 GenBank AAB97079.1 640 clathrin assembly protein long form [Rattus norvegicus]
GI:16758324 GenBank EDM18557.1 640 phosphatidylinositol binding clathrin assembly protein, isoform CRA_d [Rattus norvegicus]
GI:16758324 SwissProt O55012.1 640 RecName: Full=Phosphatidylinositol-binding clathrin assembly protein; AltName: Full=Clathrin assembly lymphoid myeloid leukemia protein; Short=rCALM [Rattus norvegicus]