Gene/Proteome Database (LMPD)

LMPD ID
LMP012836
Gene ID
89842
Species
Rattus norvegicus (Rat)
Gene Name
membrane-bound transcription factor peptidase, site 1
Gene Symbol
Mbtps1
Synonyms
S1p; Ski-1;
Alternate Names
membrane-bound transcription factor site-1 protease; site-1 protease; S1P endopeptidase; endopeptidase S1P; subtilisin/kexin isozyme 1; membrane-bound transcription factor protease, site 1;
Chromosome
19
Map Location
19q12
EC Number
3.4.21.112
Summary
widely expressed Ca2+-dependent serine proteinase for cleavage of pro-brain-derived neurotrophic factor [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Mbtps1

Proteins

membrane-bound transcription factor site-1 protease precursor
Refseq ID NP_446021
Protein GI 16758342
UniProt ID Q9WTZ3
mRNA ID NM_053569
Length 1052
MKLVNIWLLLLVVLLCGKKHLGDRLGKKAFEKAPCPSCSHLTLKVEFSSTVVEYEYIVAFNGYFTAKARNSFISSALKSSEVDNWRIIPRNNPSSDYPSDFEVIQIKEKQKAGLLTLEDHPNIKRVTPQRKVFRSLKFAESDPIVPCNETRWSQKWQSSRPLKRASLSLGSGFWHATGRHSSRRLLRAIPRQVAQTLQADVLWQMGYTGANVRVAVFDTGLSEKHPHFKNVKERTNWTNERTLDDGLGHGTFVAGVIASMRECQGFAPDAELHIFRVFTNNQVSYTSWFLDAFNYAILKKMDVLNLSIGGPDFMDHPFVDKVWELTANNVIMVSAIGNDGPLYGTLNNPADQMDVIGVGGIDFEDNIARFSSRGMTTWELPGGYGRVKPDIVTYGAGVRGSGVKGGCRALSGTSVASPVVAGAVTLLVSTVQKRELVNPASVKQALIASARRLPGVNMFEQGHGKLDLLRAYQILSSYKPQASLSPSYIDLTECPYMWPYCSQPIYYGGMPTIVNVTILNGMGVTGRIVDKPEWRPYLPQNGDNIEVAFSYSSVLWPWSGYLAISISVTKKAASWEGIAQGHIMITVASPAETELKNGAEHTSTVKLPIKVKIIPTPPRSKRVLWDQYHNLRYPPGYFPRDNLRMKNDPLDWNGDHVHTNFRDMYQHLRSMGYFVEVLGAPFTCFDATQYGTLLMVDSEEEYFPEEIAKLRRDVDNGLSLVVFSDWYNTSVMRKVKFYDENTRQWWMPDTGGANVPALNELLSVWNMGFSDGLYEGEFALANHDMYYASGCSIARFPEDGVVITQTFKDQGLEVLKQETAVVDNVPILGLYQIPAEGGGRIVLYGDSNCLDDSHRQKDCFWLLDALLQYTSYGVTPPSLSHSGNRQRPPSGAGLAPPERMEGNHLHRYSKVLEAHLGDPKPRPLPACPHLSWAKPQPLNETAPSNLWKHQKLLSIDLDKVVLPNFRSNRPQVRPLSPGESGAWDIPGGIMPGRYNQEVGQTIPVFAFLGAMVALAFFVVQISKAKSRPKRRRPRAKRPQLAQQAHPARTPSV
sig_peptide: 1..17 calculated_mol_wt: 1941 peptide sequence: MKLVNIWLLLLVVLLCG mat_peptide: 18..1052 product: membrane-bound transcription factor site-1 protease calculated_mol_wt: 115558 peptide sequence: KKHLGDRLGKKAFEKAPCPSCSHLTLKVEFSSTVVEYEYIVAFNGYFTAKARNSFISSALKSSEVDNWRIIPRNNPSSDYPSDFEVIQIKEKQKAGLLTLEDHPNIKRVTPQRKVFRSLKFAESDPIVPCNETRWSQKWQSSRPLKRASLSLGSGFWHATGRHSSRRLLRAIPRQVAQTLQADVLWQMGYTGANVRVAVFDTGLSEKHPHFKNVKERTNWTNERTLDDGLGHGTFVAGVIASMRECQGFAPDAELHIFRVFTNNQVSYTSWFLDAFNYAILKKMDVLNLSIGGPDFMDHPFVDKVWELTANNVIMVSAIGNDGPLYGTLNNPADQMDVIGVGGIDFEDNIARFSSRGMTTWELPGGYGRVKPDIVTYGAGVRGSGVKGGCRALSGTSVASPVVAGAVTLLVSTVQKRELVNPASVKQALIASARRLPGVNMFEQGHGKLDLLRAYQILSSYKPQASLSPSYIDLTECPYMWPYCSQPIYYGGMPTIVNVTILNGMGVTGRIVDKPEWRPYLPQNGDNIEVAFSYSSVLWPWSGYLAISISVTKKAASWEGIAQGHIMITVASPAETELKNGAEHTSTVKLPIKVKIIPTPPRSKRVLWDQYHNLRYPPGYFPRDNLRMKNDPLDWNGDHVHTNFRDMYQHLRSMGYFVEVLGAPFTCFDATQYGTLLMVDSEEEYFPEEIAKLRRDVDNGLSLVVFSDWYNTSVMRKVKFYDENTRQWWMPDTGGANVPALNELLSVWNMGFSDGLYEGEFALANHDMYYASGCSIARFPEDGVVITQTFKDQGLEVLKQETAVVDNVPILGLYQIPAEGGGRIVLYGDSNCLDDSHRQKDCFWLLDALLQYTSYGVTPPSLSHSGNRQRPPSGAGLAPPERMEGNHLHRYSKVLEAHLGDPKPRPLPACPHLSWAKPQPLNETAPSNLWKHQKLLSIDLDKVVLPNFRSNRPQVRPLSPGESGAWDIPGGIMPGRYNQEVGQTIPVFAFLGAMVALAFFVVQISKAKSRPKRRRPRAKRPQLAQQAHPARTPSV

Gene Information

Entrez Gene ID
89842
Gene Name
membrane-bound transcription factor peptidase, site 1
Gene Symbol
Mbtps1
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0005794 IDA:RGD C Golgi apparatus
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0004252 IDA:RGD F serine-type endopeptidase activity
GO:0008203 IEA:UniProtKB-KW P cholesterol metabolic process
GO:0006629 IBA:RefGenome P lipid metabolic process
GO:0007040 ISS:UniProtKB P lysosome organization
GO:0006508 ISS:UniProtKB P proteolysis

KEGG Pathway Links

KEGG Pathway ID Description
ko04141 Protein processing in endoplasmic reticulum
rno04141 Protein processing in endoplasmic reticulum

Domain Information

InterPro Annotations

Accession Description
IPR000209 Peptidase S8/S53 domain
IPR022398 Peptidase S8, subtilisin, His-active site
IPR015500 Peptidase S8, subtilisin-related
IPR023828 Peptidase S8, subtilisin, Ser-active site

UniProt Annotations

Entry Information

Gene Name
membrane-bound transcription factor peptidase, site 1
Protein Entry
MBTP1_RAT
UniProt ID
Q9WTZ3
Species
Rat

Comments

Comment Type Description
Catalytic Activity Processes precursors containing basic and hydrophobic/aliphatic residues at P4 and P2, respectively, with a relatively relaxed acceptance of amino acids at P1 and P3.
Cofactor Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence= ;
Enzyme Regulation Inhibited by divalent copper and zinc ions, but not by nickel or cobalt. Inhibited by its prosegment, but not smaller fragments thereof (By similarity)
Function Serine protease that catalyzes the first step in the proteolytic activation of the sterol regulatory element-binding proteins (SREBPs). Other known substrates are BDNF, GNPTAB and ATF6. Cleaves after hydrophobic or small residues, provided that Arg or Lys is in position P4. Cleaves known substrates after Arg- Ser-Val-Leu (SERBP-2), Arg-His-Leu-Leu (ATF6), Arg-Gly-Leu-Thr (BDNF) and its own propeptide after Arg-Arg-Leu-Leu. Mediates the protein cleavage of GNPTAB into subunit alpha and beta, thereby participating in biogenesis of lysosomes (By similarity)
Ptm The 148 kDa zymogen is processed progressively into two membrane-bound 120 and 106 kDa forms in the endoplasmic reticulum, and late into a secreted 98 kDa form. The propeptide is autocatalytically removed through an intramolecular cleavage after Leu-186. Further cleavage generates 14, 10, and 8 kDa intermediates (By similarity)
Similarity Belongs to the peptidase S8 family
Similarity Contains 1 peptidase S8 domain
Subcellular Location Endoplasmic reticulum membrane {ECO:0000250}; Single-pass type I membrane protein . Golgi apparatus membrane ; Single-pass type I membrane protein . Note=May sort to other organelles, including lysosomal and/or endosomal compartments
Tissue Specificity Widely expressed. In adult rat, highly expressed in anterior pituitary, thyroid and adrenal glands and in liver. In 2-day old rat, detected in developing skin, striated muscles, cardiac muscles, bones, teeth and internal organs. Highly expressed in retina, cerebellum, pituitary, submaxillary, thyroid and adrenal glands, molars, thymus, kidney and intestine.

Identical and Related Proteins

Unique RefSeq proteins for LMP012836 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16758342 RefSeq NP_446021 1052 membrane-bound transcription factor site-1 protease precursor

Identical Sequences to LMP012836 proteins

Reference Database Accession Length Protein Name
GI:16758342 EMBL CAC08993.1 1052 unnamed protein product [Rattus sp.]
GI:16758342 GenBank AAD27011.1 1052 subtilisin/kexin isozyme SKI-1 precursor [Rattus norvegicus]
GI:16758342 GenBank ABT02354.1 1052 Sequence 2 from patent US 7211424

Related Sequences to LMP012836 proteins

Reference Database Accession Length Protein Name
GI:16758342 EMBL CAC08993.1 1052 unnamed protein product [Rattus sp.]
GI:16758342 GenBank AAD27011.1 1052 subtilisin/kexin isozyme SKI-1 precursor [Rattus norvegicus]
GI:16758342 GenBank ABT02354.1 1052 Sequence 2 from patent US 7211424