Gene/Proteome Database (LMPD)

LMPD ID
LMP012844
Gene ID
113902
Species
Rattus norvegicus (Rat)
Gene Name
carboxylesterase 1D
Gene Symbol
Ces1d
Synonyms
Ces3;
Alternate Names
carboxylesterase 1D; ES-HVEL; FAEE synthase; pI 6.1 esterase; carboxylesterase 3; carboxyesterase ES-10; liver carboxylesterase 10; fatty acid ethyl ester synthase;
Chromosome
19
Map Location
19p11
EC Number
3.1.1.1
Summary
enzyme that hydrolizes long-chain fatty acids and thioesters; may play a role in lipid metabolism and/or the elimination of toxic substances [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Ces1d

Proteins

carboxylesterase 1D precursor
Refseq ID NP_579829
Protein GI 140969642
UniProt ID P16303
mRNA ID NM_133295
Length 565
MRLYPLVWLFLAACTAWGYPSSPPVVNTVKGKVLGKYVNLEGFAQPVAVFLGIPFAKPPLGSLRFAPPQPAEPWNFVKNTTSYPPMCSQDAVGGQVLSELFTNRKENIPLQFSEDCLYLNVYTPADLTKNSRLPVMVWIHGGGLVVGGASTYDGQVLSAHENVVVVTIQYRLGIWGFFSTGDEHSQGNWGHLDQVAALHWVQDNIANFGGNPGSVTIFGESAGGFSVSALVLSPLAKNLFHRAISESGVVLTSALITTDSKPIANLIATLSGCKTTTSAVMVHCLRQKTEDELLETSLKLNLFKLDLLGNPKESYPFLPTVIDGVVLPKTPEEILTEKSFNTVPYIVGINKQEFGWIIPTLMGYPLSEGKLDQKTAKSLLWKSYPTLKISEKMIPVVAEKYFGGTDDPAKRKDLFQDLVADVMFGVPSVMVSRSHRDAGAPTFMYEFEYRPSFVSAMRPKTVIGDHGDELFSVFGSPFLKDGASEEETNLSKMVMKYWANFARNGSPNGGGLPHWPEYDQKEGYLKIGASTQAAQRLKDKEVAFWSELRAKEAAEEPSHWKHVEL
sig_peptide: 1..18 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 2112 peptide sequence: MRLYPLVWLFLAACTAWG mat_peptide: 19..565 product: Carboxylesterase 1D experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (P16303.2) calculated_mol_wt: 60057 peptide sequence: YPSSPPVVNTVKGKVLGKYVNLEGFAQPVAVFLGIPFAKPPLGSLRFAPPQPAEPWNFVKNTTSYPPMCSQDAVGGQVLSELFTNRKENIPLQFSEDCLYLNVYTPADLTKNSRLPVMVWIHGGGLVVGGASTYDGQVLSAHENVVVVTIQYRLGIWGFFSTGDEHSQGNWGHLDQVAALHWVQDNIANFGGNPGSVTIFGESAGGFSVSALVLSPLAKNLFHRAISESGVVLTSALITTDSKPIANLIATLSGCKTTTSAVMVHCLRQKTEDELLETSLKLNLFKLDLLGNPKESYPFLPTVIDGVVLPKTPEEILTEKSFNTVPYIVGINKQEFGWIIPTLMGYPLSEGKLDQKTAKSLLWKSYPTLKISEKMIPVVAEKYFGGTDDPAKRKDLFQDLVADVMFGVPSVMVSRSHRDAGAPTFMYEFEYRPSFVSAMRPKTVIGDHGDELFSVFGSPFLKDGASEEETNLSKMVMKYWANFARNGSPNGGGLPHWPEYDQKEGYLKIGASTQAAQRLKDKEVAFWSELRAKEAAEEPSHWKHVEL

Gene Information

Entrez Gene ID
113902
Gene Name
carboxylesterase 1D
Gene Symbol
Ces1d
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 ISS:UniProtKB C cytosol
GO:0005788 ISS:UniProtKB C endoplasmic reticulum lumen
GO:0005811 ISS:UniProtKB C lipid particle
GO:0052689 IMP:RGD F carboxylic ester hydrolase activity
GO:0030339 IEA:UniProtKB-EC F fatty-acyl-ethyl-ester synthase activity
GO:0004771 ISS:UniProtKB F sterol esterase activity
GO:0004806 ISS:UniProtKB F triglyceride lipase activity
GO:0046464 ISS:UniProtKB P acylglycerol catabolic process

KEGG Pathway Links

KEGG Pathway ID Description
ko00983 Drug metabolism - other enzymes
rno00983 Drug metabolism - other enzymes
rno01100 Metabolic pathways

Domain Information

InterPro Annotations

Accession Description
IPR029058 Alpha/Beta hydrolase fold
IPR002018 Carboxylesterase, type B
IPR019826 Carboxylesterase type B, active site
IPR019819 Carboxylesterase type B, conserved site

UniProt Annotations

Entry Information

Gene Name
carboxylesterase 1D
Protein Entry
CES1D_RAT
UniProt ID
P16303
Species
Rat

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=0.71 M for oleic acid ; Vmax=1482 nmol/h/mg enzyme toward oleic acid ; pH dependence: Optimum pH for FAEE synthesis is 7.0. Optimum pH for PNPB- hydrolyzing activity is 6-8. ;
Catalytic Activity A carboxylic ester + H(2)O = an alcohol + a carboxylate. {ECO:0000255|PROSITE-ProRule:PRU10039, ECO:0000269|PubMed:1429692}.
Catalytic Activity A long-chain-fatty-acyl ethyl ester + H(2)O = a long-chain-fatty acid + ethanol
Enzyme Regulation FAEE-synthesizing and PNPB-hydrolyzing activities are both inhibited by DFP
Function Major lipase in white adipose tissue (By similarity). Involved in the metabolism of xenobiotics and of natural substrates. Hydrolyzes triacylglycerols and monoacylglycerols, with a preference for monoacylglycerols. The susceptibility of the substrate increases with decreasing acyl chain length of the fatty acid moiety. Catalyzes the synthesis of fatty acid ethyl esters
Similarity Belongs to the type-B carboxylesterase/lipase family
Subcellular Location Endoplasmic reticulum lumen. Cytoplasm, cytosol {ECO:0000250}. Lipid droplet .
Subunit Homotrimer
Tissue Specificity Detected in liver, lung and testis, but not in kidney (at protein level)

Identical and Related Proteins

Unique RefSeq proteins for LMP012844 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
140969642 RefSeq NP_579829 565 carboxylesterase 1D precursor

Identical Sequences to LMP012844 proteins

Reference Database Accession Length Protein Name
GI:140969642 GenBank EDL87578.1 565 rCG44263 [Rattus norvegicus]

Related Sequences to LMP012844 proteins

Reference Database Accession Length Protein Name
GI:140969642 EMBL CAA46391.1 565 carboxylesterase [Rattus rattus]
GI:140969642 GenBank EDL87578.1 565 rCG44263 [Rattus norvegicus]
GI:140969642 SwissProt P16303.2 565 RecName: Full=Carboxylesterase 1D; AltName: Full=Carboxyesterase ES-10; AltName: Full=Carboxylesterase 3; AltName: Full=ES-HVEL; AltName: Full=Fatty acid ethyl ester synthase; Short=FAEE synthase; AltName: Full=Liver carboxylesterase 10; AltName: Full=pI 6.1 esterase; Flags: Precursor [Rattus norvegicus]