Gene/Proteome Database (LMPD)

LMPD ID
LMP012848
Gene ID
113976
Species
Rattus norvegicus (Rat)
Gene Name
acyl-CoA synthetase long-chain family member 4
Gene Symbol
Acsl4
Synonyms
Acs4; Facl4;
Alternate Names
long-chain-fatty-acid--CoA ligase 4; LACS 4; long-chain acyl-CoA synthetase 4; fatty acid-Coenzyme A ligase long chain 4; fatty acid Coenzyme A ligase, long chain 4; fatty acid-Coenzyme A ligase, long chain 4;
Chromosome
X
Map Location
Xq14
EC Number
6.2.1.3
Summary
catalyzes the ligation of a fatty acid to produce acyl Co-A; acts on a wide range of fatty acids with preferential activity for arachidonate and eicosapentaenoate; plays a role in fatty acid metabolism [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Acsl4

Proteins

long-chain-fatty-acid--CoA ligase 4
Refseq ID NP_446075
Protein GI 16758426
UniProt ID O35547
mRNA ID NM_053623
Length 670
MAKRIKAKPTSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVAKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWINYLEVNCRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKAALLDINCVKHIIYVDNKTINRAEYPEGLEIHSMQSVEELGSKPENSSIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYIQKTLFKIGYDYKLEQIKKGYDAPLCNLILFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTVHDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYSVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKKLTLLAQQKGVEGSWVDICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELKNHYLKDIERMYGGK

Gene Information

Entrez Gene ID
113976
Gene Name
acyl-CoA synthetase long-chain family member 4
Gene Symbol
Acsl4
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0070062 IEA:Ensembl C extracellular vesicular exosome
GO:0005811 IEA:Ensembl C lipid particle
GO:0031966 IDA:RGD C mitochondrial membrane
GO:0043025 IDA:RGD C neuronal cell body
GO:0005777 IDA:RGD C peroxisome
GO:0047676 IEA:Ensembl F arachidonate-CoA ligase activity
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0004467 IDA:RGD F long-chain fatty acid-CoA ligase activity
GO:0031957 IEA:Ensembl F very long-chain fatty acid-CoA ligase activity
GO:0060996 IMP:RGD P dendritic spine development
GO:0060136 IEA:Ensembl P embryonic process involved in female pregnancy
GO:0006631 TAS:RGD P fatty acid metabolic process
GO:0015908 IGI:RGD P fatty acid transport
GO:0008610 IEA:Ensembl P lipid biosynthetic process
GO:0001676 IDA:GOC P long-chain fatty acid metabolic process
GO:0032307 IEA:Ensembl P negative regulation of prostaglandin secretion
GO:0030307 IEA:Ensembl P positive regulation of cell growth
GO:0070672 IEP:RGD P response to interleukin-15
GO:0007584 IEP:RGD P response to nutrient
GO:0006641 IGI:RGD P triglyceride metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
ko04920 Adipocytokine signaling pathway
rno04920 Adipocytokine signaling pathway
M00086 beta-Oxidation, acyl-CoA synthesis
ko00061 Fatty acid biosynthesis
rno00061 Fatty acid biosynthesis
ko00071 Fatty acid degradation
rno00071 Fatty acid degradation
ko01212 Fatty acid metabolism
rno01212 Fatty acid metabolism
rno01100 Metabolic pathways
ko04146 Peroxisome
rno04146 Peroxisome
ko03320 PPAR signaling pathway
rno03320 PPAR signaling pathway

REACTOME Pathway Links

REACTOME Pathway ID Description
5953288 Fatty acid, triacylglycerol, and ketone body metabolism
5953463 Fatty Acyl-CoA Biosynthesis
5953250 Metabolism
5953289 Metabolism of lipids and lipoproteins
5953471 Synthesis of very long-chain fatty acyl-CoAs
5953464 Triglyceride Biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR020845 AMP-binding, conserved site
IPR000873 AMP-dependent synthetase/ligase

UniProt Annotations

Entry Information

Gene Name
acyl-CoA synthetase long-chain family member 4
Protein Entry
ACSL4_RAT
UniProt ID
O35547
Species
Rat

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=34 uM for ATP ; KM=4.1 uM for CoA ; KM=5.4 uM for palmitate ; KM=19.5 uM for oleate ; KM=10 uM for arachidonate ; Vmax=2800 nmol/min/mg enzyme with palmitate as substrate ; Vmax=673 nmol/min/mg enzyme with oleate as substrate ; Vmax=4200 nmol/min/mg enzyme with arachidonate as substrate ;
Catalytic Activity ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence= ;
Function Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.
Miscellaneous 5 rat isozymes encoded by different genes have been described. ACSL6 corresponds to isozyme 2 (ACS2)
Similarity Belongs to the ATP-dependent AMP-binding enzyme family

Identical and Related Proteins

Unique RefSeq proteins for LMP012848 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16758426 RefSeq NP_446075 670 long-chain-fatty-acid--CoA ligase 4

Identical Sequences to LMP012848 proteins

Reference Database Accession Length Protein Name
GI:16758426 GenBank EDL85207.1 670 acyl-CoA synthetase long-chain family member 4, isoform CRA_a [Rattus norvegicus]
GI:16758426 GenBank EDL85208.1 670 acyl-CoA synthetase long-chain family member 4, isoform CRA_a [Rattus norvegicus]
GI:16758426 RefSeq XP_006257377.1 670 PREDICTED: long-chain-fatty-acid--CoA ligase 4 isoform X1 [Rattus norvegicus]
GI:16758426 RefSeq XP_006257378.1 670 PREDICTED: long-chain-fatty-acid--CoA ligase 4 isoform X1 [Rattus norvegicus]

Related Sequences to LMP012848 proteins

Reference Database Accession Length Protein Name
GI:16758426 GenBank EDL85207.1 670 acyl-CoA synthetase long-chain family member 4, isoform CRA_a [Rattus norvegicus]
GI:16758426 GenBank EDL85208.1 670 acyl-CoA synthetase long-chain family member 4, isoform CRA_a [Rattus norvegicus]
GI:16758426 RefSeq XP_006257377.1 670 PREDICTED: long-chain-fatty-acid--CoA ligase 4 isoform X1 [Rattus norvegicus]