Gene/Proteome Database (LMPD)

LMPD ID
LMP012851
Gene ID
114024
Species
Rattus norvegicus (Rat)
Gene Name
acyl-CoA synthetase long-chain family member 3
Gene Symbol
Acsl3
Synonyms
Acs3; Facl3;
Alternate Names
long-chain-fatty-acid--CoA ligase 3; LACS 3; brain acyl-CoA synthetase II; long-chain acyl-CoA synthetase 3; fatty acid Coenzyme A ligase, long chain 3;
Chromosome
9
Map Location
9q33
EC Number
6.2.1.3
Summary
catalyzes initial reaction of fatty acid metabolism to produce Acyl-CoA; plays a key role in lipid metabolism; may mediate the synthesis of brain lipids [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Acsl3

Proteins

long-chain-fatty-acid--CoA ligase 3
Refseq ID NP_476448
Protein GI 16923952
UniProt ID Q63151
mRNA ID NM_057107
Length 720
MNNHVSSTPSTMKLKQTIHPILLYFIHFIISLYTILTYIPFYFLCESKQEKPNHIKAKPVSSKPDSAYRSVNSMDGLASVLYPGCDTLDKVFMYAKNKFKDKRLLGTREILNEEDEIQPNGKVFKKVILGHYNWLSYEDVFIRALDFGNGLQMLGQKPKANIAIFCETRAEWMIAAQACFMYNFQLVTLYATLGGPAIVHGLNETEVTNIITSKELLQTKLKDIVSLVPRLRHIITVDGKPPTWSEFPKGVIVHTMAAVQALGVKADVDKKAHSKPLPSDIAVIMYTSGSTGIPKGVMISHSNIIASITGMARRIPRLGEEDVYIGYLPLAHVLELSAELVCLSHGCRIGYSSPQTLADQSSKIKKGSKGDTSVLKPTLMAAVPEIMDRIYKNVMNKVNEMSAFQRNLFILAYNYKMEQISKGCSTPLCDRFVFRNVRRLLGGNIRVLLCGGAPLSATTQRFMNICFCCPVGQGYGLTESTGAGTITEVWDYNTGRVGAPLVCCEIKLKNWEEGGYFNTDKPHPRGEILIGGQNVTMGYYKNEAKTKADFFEDENGQRWLCTGDIGEFDPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPLIDNICAYANSYHSYVIGFVVPNQKELTELARTKGFNGTWEELCNSSEMENEVLKVLSEAAISASLEKFEIPLKIRLSPDPWTPETGLVTDAFKLKRKELKTHYQADIERMYGRK

Gene Information

Entrez Gene ID
114024
Gene Name
acyl-CoA synthetase long-chain family member 3
Gene Symbol
Acsl3
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0005794 IEA:Ensembl C Golgi apparatus
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0005811 IEA:Ensembl C lipid particle
GO:0005741 IEA:UniProtKB-KW C mitochondrial outer membrane
GO:0048471 IEA:Ensembl C perinuclear region of cytoplasm
GO:0005777 IEA:UniProtKB-KW C peroxisome
GO:0003987 TAS:RGD F acetate-CoA ligase activity
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0004467 IDA:RGD F long-chain fatty acid-CoA ligase activity
GO:0007420 IEP:RGD P brain development
GO:0006633 IDA:RGD P fatty acid biosynthetic process
GO:0006631 TAS:RGD P fatty acid metabolic process
GO:0006629 TAS:RGD P lipid metabolic process
GO:0044539 IEA:Ensembl P long-chain fatty acid import
GO:0001676 IDA:GOC P long-chain fatty acid metabolic process
GO:0008152 TAS:RGD P metabolic process
GO:0042998 IEA:Ensembl P positive regulation of Golgi to plasma membrane protein transport
GO:2001247 IEA:Ensembl P positive regulation of phosphatidylcholine biosynthetic process
GO:0051047 IEA:Ensembl P positive regulation of secretion
GO:0007584 IEP:RGD P response to nutrient
GO:0014070 IEP:RGD P response to organic cyclic compound
GO:0034379 IEA:Ensembl P very-low-density lipoprotein particle assembly

KEGG Pathway Links

KEGG Pathway ID Description
ko04920 Adipocytokine signaling pathway
rno04920 Adipocytokine signaling pathway
M00086 beta-Oxidation, acyl-CoA synthesis
ko00061 Fatty acid biosynthesis
rno00061 Fatty acid biosynthesis
ko00071 Fatty acid degradation
rno00071 Fatty acid degradation
ko01212 Fatty acid metabolism
rno01212 Fatty acid metabolism
rno01100 Metabolic pathways
ko04146 Peroxisome
rno04146 Peroxisome
ko03320 PPAR signaling pathway
rno03320 PPAR signaling pathway

Domain Information

InterPro Annotations

Accession Description
IPR020845 AMP-binding, conserved site
IPR000873 AMP-dependent synthetase/ligase

UniProt Annotations

Entry Information

Gene Name
acyl-CoA synthetase long-chain family member 3
Protein Entry
ACSL3_RAT
UniProt ID
Q63151
Species
Rat

Comments

Comment Type Description
Alternative Products Event=Alternative initiation; Named isoforms=2; Name=Long; IsoId=Q63151-1; Sequence=Displayed; Name=Short; IsoId=Q63151-2; Sequence=VSP_018649;
Biophysicochemical Properties Kinetic parameters: KM=402 uM for ATP ; KM=3.0 uM for CoA ; KM=4.3 uM for palmitate ; KM=5.1 uM for oleate ; KM=8.9 uM for arachidonate ; Vmax=2306 nmol/min/mg enzyme with palmitate as substrate ; Vmax=2399 nmol/min/mg enzyme with oleate as substrate ; Vmax=4022 nmol/min/mg enzyme with arachidonate as substrate ;
Catalytic Activity ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence= ;
Developmental Stage Detected 5 days after birth, increased to a maximal level at 15 days, and then decreased gradually to 10% of its maximum level in adult.
Function Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 is required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins) (By similarity). Has mainly an anabolic role in energy metabolism. Mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Both isoforms exhibit the same level of activity. {ECO:0000250, ECO:0000269|PubMed:19737935}.
Miscellaneous 5 rat isozymes encoded by different genes have been described. ACSL6 corresponds to isozyme 2 (ACS2)
Similarity Belongs to the ATP-dependent AMP-binding enzyme family
Subcellular Location Mitochondrion outer membrane ; Single-pass type III membrane protein . Peroxisome membrane ; Single-pass type III membrane protein {ECO:0000250}. Microsome membrane ; Single-pass type III membrane protein . Endoplasmic reticulum membrane ; Single-pass type III membrane protein .
Tissue Specificity Predominantly expressed in the brain, and to a much lesser extent, in lung, adrenal gland, kidney, small intestine, and adipose tissue but not detected in heart or liver.

Identical and Related Proteins

Unique RefSeq proteins for LMP012851 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16923952 RefSeq NP_476448 720 long-chain-fatty-acid--CoA ligase 3

Identical Sequences to LMP012851 proteins

Reference Database Accession Length Protein Name
GI:16923952 DBBJ BAA06340.1 720 brain acyl-CoA synthtase II [Rattus norvegicus]
GI:16923952 GenBank EDL75474.1 720 acyl-CoA synthetase long-chain family member 3, isoform CRA_a [Rattus norvegicus]
GI:16923952 GenBank EDL75475.1 720 acyl-CoA synthetase long-chain family member 3, isoform CRA_a [Rattus norvegicus]
GI:16923952 SwissProt Q63151.1 720 RecName: Full=Long-chain-fatty-acid--CoA ligase 3; AltName: Full=Brain acyl-CoA synthetase II; AltName: Full=Long-chain acyl-CoA synthetase 3; Short=LACS 3 [Rattus norvegicus]

Related Sequences to LMP012851 proteins

Reference Database Accession Length Protein Name
GI:16923952 DBBJ BAA06340.1 720 brain acyl-CoA synthtase II [Rattus norvegicus]
GI:16923952 GenBank EDL75475.1 720 acyl-CoA synthetase long-chain family member 3, isoform CRA_a [Rattus norvegicus]
GI:16923952 SwissProt Q63151.1 720 RecName: Full=Long-chain-fatty-acid--CoA ligase 3; AltName: Full=Brain acyl-CoA synthetase II; AltName: Full=Long-chain acyl-CoA synthetase 3; Short=LACS 3 [Rattus norvegicus]