Gene/Proteome Database (LMPD)

LMPD ID
LMP012858
Gene ID
Species
Rattus norvegicus (Rat)
Gene Name
N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
Gene Symbol
Alternate Names
neutral ceramidase; NCDase; N-CDase; acylsphingosine deacylase 2; neutral/alkaline ceramidase; N-acylsphingosine amidohydrolase 2;
Chromosome
1
Map Location
1q52
EC Number
3.5.1.23
Summary
membrane-bound neutral ceramidase; hydrolyzes the N-acyl linkage of ceramide to produce sphingosine [RGD, Feb 2006]
Orthologs

Proteins

neutral ceramidase
Refseq ID NP_446098
Protein GI 16758458
UniProt ID Q91XT9
mRNA ID NM_053646
Length 761
MAKRTFSSLEAFLIFLLVMMTAITVALLTLLFVTSGTIENHKDSGNHWVSTTQGPTTTQSSPTTQTPTTQTPDLPPSQNFSGYYIGVGRADCTGQVSDINLMGYGKNGQNAQGLLTRLFSRAFILADPDGSNRMAFVSVELCMISQRLRLEVLKRLQSKYGSLYRRDNVILSATHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIVKSIDIAHTNLKPGKVLINKGNVANVQINRSPSSYLQNPPSERARYSSDTDKEMVVLKLVDLNGEDLGLISWFAVHPVSMNNSNHLVNSDNMGYAAYLFEQEKNRGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKSTCPSGGPSMCMASGPGQDMFESTHIIGRVIYQKAKELHASASQEVTGPVLTAHQWVNMTDVSVQLNATHTVKTCKAALGYSFAAGTIDGVSGLNITQGTTEGNLFWDTLRDQLLGKPSEEIIECQKPKPILIHTGELTKPHPWQPDIVDIQIVTLGSLAIAAIPGEFTTMSGRRLREAVKKEFALYGMKDMTVVIAGLSNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRALAKAIATDTVANMSSGPEPPFFKNLIGSLIPNIADRAPIGKQFGDVLQPAKPEYRVGEVVEVVFVGANPKNSAENQTHQTFLTVEKYEDSVANWQIMHNDASWETRFYWHKGVLGLSNATIHWHIPDTALPGVYRIRYFGHNRKQELLKPAVILAFEGISSPFEIVTT

Gene Information

Entrez Gene ID
Gene Name
N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
Gene Symbol
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0005739 IEA:Ensembl C mitochondrion
GO:0005886 IEA:UniProtKB-KW C plasma membrane
GO:0017040 IEA:UniProtKB-EC F ceramidase activity
GO:0006915 IEA:UniProtKB-KW P apoptotic process
GO:0010033 IDA:RGD P response to organic substance
GO:0006665 IEA:UniProtKB-KW P sphingolipid metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
rno01100 Metabolic pathways
ko00600 Sphingolipid metabolism
rno00600 Sphingolipid metabolism
M00099 Sphingosine biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
5954485 Glycosphingolipid metabolism
5953250 Metabolism
5953289 Metabolism of lipids and lipoproteins
5954267 Sphingolipid metabolism

Domain Information

InterPro Annotations

Accession Description
IPR006823 Neutral/alkaline nonlysosomal ceramidase

UniProt Annotations

Entry Information

Gene Name
N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
Protein Entry
ASAH2_RAT
UniProt ID
Species
Rat

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=71.4 uM for octanoyl-sphingosine {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; KM=66 uM for palmitoyl-sphingosine {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; KM=1.29 M for C16-ceramide {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; KM=3.84 M for dihydroceramide {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; Vmax=160 umol/min/mg enzyme with octanoyl-sphingosine as substrate {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; Vmax=16 umol/min/mg enzyme with palmitoyl-sphingosine as substrate {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; Vmax=4.4 umol/min/mg enzyme with C16-ceramide as substrate {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; Vmax=1.2 umol/min/mg enzyme with dihydroceramide as substrate {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; pH dependence: Optimum pH is 7-10. {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782};
Catalytic Activity N-acylsphingosine + H(2)O = a carboxylate + sphingosine. {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816}.
Enzyme Regulation Inhibited by dithiothreitol (DTT), 2- mercaptoethanol, Zn(2+) and Cu(2+).
Function Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract
Induction By interleukin-1-beta in renal mesengial cells
Ptm N-glycosylated. Required for enzyme activity.
Ptm O-glycosylated. Required to retain it as a type II membrane protein at the cell surface.
Ptm Phosphorylated. May prevent ubiquitination and subsequent degradation. {ECO:0000269|PubMed:12359735, ECO:0000269|PubMed:12482609}.
Ptm Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by nitric oxid
Similarity Belongs to the neutral ceramidase family
Subcellular Location Cell membrane {ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:12499379}; Single-pass type II membrane protein . Note=The neutral ceramidase soluble form is a secreted protein.
Tissue Specificity Widelky expressed. Highly expressed in brain, kidney and heart. Expressed at lower level in other tissues such as liver. Localizes in the epithelia of the jejunum and ileum

Identical and Related Proteins

Unique RefSeq proteins for LMP012858 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16758458 RefSeq NP_446098 761 neutral ceramidase

Identical Sequences to LMP012858 proteins

Reference Database Accession Length Protein Name
GI:16758458 RefSeq XP_006231330.1 761 PREDICTED: neutral ceramidase isoform X1 [Rattus norvegicus]
GI:16758458 RefSeq XP_006231332.1 761 PREDICTED: neutral ceramidase isoform X1 [Rattus norvegicus]
GI:16758458 RefSeq XP_008758541.1 761 PREDICTED: neutral ceramidase isoform X1 [Rattus norvegicus]
GI:16758458 RefSeq XP_008758542.1 761 PREDICTED: neutral ceramidase isoform X1 [Rattus norvegicus]

Related Sequences to LMP012858 proteins

Reference Database Accession Length Protein Name
GI:16758458 RefSeq XP_006231325.1 761 PREDICTED: neutral ceramidase isoform X1 [Rattus norvegicus]
GI:16758458 RefSeq XP_006231329.1 761 PREDICTED: neutral ceramidase isoform X1 [Rattus norvegicus]
GI:16758458 RefSeq XP_006231330.1 761 PREDICTED: neutral ceramidase isoform X1 [Rattus norvegicus]