Gene/Proteome Database (LMPD)
LMPD ID
LMP012858
Gene ID
Species
Rattus norvegicus (Rat)
Gene Name
N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
Gene Symbol
Alternate Names
neutral ceramidase; NCDase; N-CDase; acylsphingosine deacylase 2; neutral/alkaline ceramidase; N-acylsphingosine amidohydrolase 2;
Chromosome
1
Map Location
1q52
EC Number
3.5.1.23
Summary
membrane-bound neutral ceramidase; hydrolyzes the N-acyl linkage of ceramide to produce sphingosine [RGD, Feb 2006]
Orthologs
Proteins
| neutral ceramidase | |
|---|---|
| Refseq ID | NP_446098 |
| Protein GI | 16758458 |
| UniProt ID | Q91XT9 |
| mRNA ID | NM_053646 |
| Length | 761 |
| MAKRTFSSLEAFLIFLLVMMTAITVALLTLLFVTSGTIENHKDSGNHWVSTTQGPTTTQSSPTTQTPTTQTPDLPPSQNFSGYYIGVGRADCTGQVSDINLMGYGKNGQNAQGLLTRLFSRAFILADPDGSNRMAFVSVELCMISQRLRLEVLKRLQSKYGSLYRRDNVILSATHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIVKSIDIAHTNLKPGKVLINKGNVANVQINRSPSSYLQNPPSERARYSSDTDKEMVVLKLVDLNGEDLGLISWFAVHPVSMNNSNHLVNSDNMGYAAYLFEQEKNRGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKSTCPSGGPSMCMASGPGQDMFESTHIIGRVIYQKAKELHASASQEVTGPVLTAHQWVNMTDVSVQLNATHTVKTCKAALGYSFAAGTIDGVSGLNITQGTTEGNLFWDTLRDQLLGKPSEEIIECQKPKPILIHTGELTKPHPWQPDIVDIQIVTLGSLAIAAIPGEFTTMSGRRLREAVKKEFALYGMKDMTVVIAGLSNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRALAKAIATDTVANMSSGPEPPFFKNLIGSLIPNIADRAPIGKQFGDVLQPAKPEYRVGEVVEVVFVGANPKNSAENQTHQTFLTVEKYEDSVANWQIMHNDASWETRFYWHKGVLGLSNATIHWHIPDTALPGVYRIRYFGHNRKQELLKPAVILAFEGISSPFEIVTT | |
Gene Information
Entrez Gene ID
Gene Name
N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
Gene Symbol
Species
Rattus norvegicus
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0016021 | IEA:UniProtKB-KW | C | integral component of membrane |
| GO:0005739 | IEA:Ensembl | C | mitochondrion |
| GO:0005886 | IEA:UniProtKB-KW | C | plasma membrane |
| GO:0017040 | IEA:UniProtKB-EC | F | ceramidase activity |
| GO:0006915 | IEA:UniProtKB-KW | P | apoptotic process |
| GO:0010033 | IDA:RGD | P | response to organic substance |
| GO:0006665 | IEA:UniProtKB-KW | P | sphingolipid metabolic process |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| rno01100 | Metabolic pathways |
| ko00600 | Sphingolipid metabolism |
| rno00600 | Sphingolipid metabolism |
| M00099 | Sphingosine biosynthesis |
REACTOME Pathway Links
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR006823 | Neutral/alkaline nonlysosomal ceramidase |
UniProt Annotations
Entry Information
Gene Name
N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
Protein Entry
ASAH2_RAT
UniProt ID
Species
Rat
Comments
| Comment Type | Description |
|---|---|
| Biophysicochemical Properties | Kinetic parameters: KM=71.4 uM for octanoyl-sphingosine {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; KM=66 uM for palmitoyl-sphingosine {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; KM=1.29 M for C16-ceramide {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; KM=3.84 M for dihydroceramide {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; Vmax=160 umol/min/mg enzyme with octanoyl-sphingosine as substrate {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; Vmax=16 umol/min/mg enzyme with palmitoyl-sphingosine as substrate {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; Vmax=4.4 umol/min/mg enzyme with C16-ceramide as substrate {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; Vmax=1.2 umol/min/mg enzyme with dihydroceramide as substrate {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; pH dependence: Optimum pH is 7-10. {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:15217782}; |
| Catalytic Activity | N-acylsphingosine + H(2)O = a carboxylate + sphingosine. {ECO:0000269|PubMed:10488143, ECO:0000269|PubMed:11328816}. |
| Enzyme Regulation | Inhibited by dithiothreitol (DTT), 2- mercaptoethanol, Zn(2+) and Cu(2+). |
| Function | Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract |
| Induction | By interleukin-1-beta in renal mesengial cells |
| Ptm | N-glycosylated. Required for enzyme activity. |
| Ptm | O-glycosylated. Required to retain it as a type II membrane protein at the cell surface. |
| Ptm | Phosphorylated. May prevent ubiquitination and subsequent degradation. {ECO:0000269|PubMed:12359735, ECO:0000269|PubMed:12482609}. |
| Ptm | Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by nitric oxid |
| Similarity | Belongs to the neutral ceramidase family |
| Subcellular Location | Cell membrane {ECO:0000269|PubMed:11328816, ECO:0000269|PubMed:12499379}; Single-pass type II membrane protein . Note=The neutral ceramidase soluble form is a secreted protein. |
| Tissue Specificity | Widelky expressed. Highly expressed in brain, kidney and heart. Expressed at lower level in other tissues such as liver. Localizes in the epithelia of the jejunum and ileum |
Identical and Related Proteins
Unique RefSeq proteins for LMP012858 (as displayed in Record Overview)
Identical Sequences to LMP012858 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:16758458 | RefSeq | XP_006231330.1 | 761 | PREDICTED: neutral ceramidase isoform X1 [Rattus norvegicus] |
| GI:16758458 | RefSeq | XP_006231332.1 | 761 | PREDICTED: neutral ceramidase isoform X1 [Rattus norvegicus] |
| GI:16758458 | RefSeq | XP_008758541.1 | 761 | PREDICTED: neutral ceramidase isoform X1 [Rattus norvegicus] |
| GI:16758458 | RefSeq | XP_008758542.1 | 761 | PREDICTED: neutral ceramidase isoform X1 [Rattus norvegicus] |
Related Sequences to LMP012858 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:16758458 | RefSeq | XP_006231325.1 | 761 | PREDICTED: neutral ceramidase isoform X1 [Rattus norvegicus] |
| GI:16758458 | RefSeq | XP_006231329.1 | 761 | PREDICTED: neutral ceramidase isoform X1 [Rattus norvegicus] |
| GI:16758458 | RefSeq | XP_006231330.1 | 761 | PREDICTED: neutral ceramidase isoform X1 [Rattus norvegicus] |