Gene/Proteome Database (LMPD)

LMPD ID
LMP012860
Gene ID
114114
Species
Rattus norvegicus (Rat)
Gene Name
dynamin 1-like
Gene Symbol
Dnm1l
Synonyms
DLP1; Drp1; Dnml1;
Alternate Names
dynamin-1-like protein; C-terminal region; N-terminal region; dynamin-like protein 1; dynamin-related protein 1;
Chromosome
11
Map Location
11q23
EC Number
3.6.5.5
Summary
human homolog is a GTPase that is necessary for peroxisome division [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Dnm1l

Proteins

dynamin-1-like protein
Refseq ID NP_446107
Protein GI 77917614
UniProt ID O35303
mRNA ID NM_053655
Length 716
MEALIPVINKLQDVFNTVGADIIQLPQIVVVGTQSSGKSSVLESLVGRDLLPRGTGVVTRRPLILQLVHVSPEDKRKTTGEENGVEAEEWGKFLHTKNKLYTDFDEIRQEIENETERISGNNKGVSPEPIHLKVFSPNVVNLTLVDLPGMTKVPVGDQPKDIELQIRELILRFISNPNSIILAVTAANTDMATSEALKISREVDPDGRRTLAVITKLDLMDAGTDAMDVLMGRVIPVKLGIIGVVNRSQLDINNKKSVTDSIRDEYAFLQKKYPSLANRNGTKYLARTLNRLLMHHIRDCLPELKTRINVLAAQYQSLLNSYGEPVDDKSATLLQLITKFATEYCNTIEGTAKYIETSELCGGARICYIFHETFGRTLESVDPLGGLNTIDILTAIRNATGPRPALFVPEVSFELLVKRQIKRLEEPSLRCVELVHEEMQRIIQHCSNYSTQELLRFPKLHDAIVEVVTCLLRKRLPVTNEMVHNLVAIELAYINTKHPDFADACGLMNNNIEEQRRNRLARELPSAVSRDKLIQDNRRETKNVASAGGGIGDGGRIGDGGQEPTTGNWRGMLKTSKAEELLAEEKSKPIPIMPASPQKGHAVNLLDVPVPVARKLSAREQRDCEVIERLIKSYFLIVRKNIQDSVPKAVMHFLVNHVKDTLQSELVGQLYKSSLLDDLLTESEDMAQRRKEAADMLKALQGASQIIAEIRETHLW

Gene Information

Entrez Gene ID
114114
Gene Name
dynamin 1-like
Gene Symbol
Dnm1l
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005794 IEA:UniProtKB-KW C Golgi apparatus
GO:0030054 IEA:UniProtKB-KW C cell junction
GO:0005905 IEA:UniProtKB-KW C coated pit
GO:0031410 IEA:UniProtKB-KW C cytoplasmic vesicle
GO:0005829 ISS:UniProtKB C cytosol
GO:0043231 IDA:RGD C intracellular membrane-bounded organelle
GO:0005874 IEA:Ensembl C microtubule
GO:0005741 IEA:UniProtKB-KW C mitochondrial outer membrane
GO:0005739 ISS:UniProtKB C mitochondrion
GO:0048471 IEA:Ensembl C perinuclear region of cytoplasm
GO:0005777 ISS:UniProtKB C peroxisome
GO:0045202 IEA:UniProtKB-KW C synapse
GO:0005525 IEA:UniProtKB-KW F GTP binding
GO:0003924 ISS:UniProtKB F GTPase activity
GO:0008289 IEA:UniProtKB-KW F lipid binding
GO:0042803 ISS:UniProtKB F protein homodimerization activity
GO:0006184 ISS:UniProtKB P GTP catabolic process
GO:0003374 ISS:UniProtKB P dynamin polymerization involved in mitochondrial fission
GO:0006897 IEA:UniProtKB-KW P endocytosis
GO:0090149 IEA:Ensembl P membrane fission involved in mitochondrial fission
GO:0061025 IEA:Ensembl P membrane fusion
GO:0000266 ISS:UniProtKB P mitochondrial fission
GO:0043653 IEA:Ensembl P mitochondrial fragmentation involved in apoptotic process
GO:0070584 IEA:Ensembl P mitochondrion morphogenesis
GO:0070266 ISS:UniProtKB P necroptotic process
GO:0016559 ISS:UniProtKB P peroxisome fission
GO:0043065 IEA:Ensembl P positive regulation of apoptotic process
GO:2001244 IEA:Ensembl P positive regulation of intrinsic apoptotic signaling pathway
GO:0050714 IEA:Ensembl P positive regulation of protein secretion
GO:0090200 IEA:Ensembl P positive regulation of release of cytochrome c from mitochondria
GO:0051289 IEA:Ensembl P protein homotetramerization
GO:0070585 IEA:Ensembl P protein localization to mitochondrion
GO:1900063 IEA:Ensembl P regulation of peroxisome organization
GO:0032459 IEA:Ensembl P regulation of protein oligomerization
GO:0001836 IEA:Ensembl P release of cytochrome c from mitochondria

KEGG Pathway Links

KEGG Pathway ID Description
ko04668 TNF signaling pathway
rno04668 TNF signaling pathway

REACTOME Pathway Links

REACTOME Pathway ID Description
5953314 Apoptosis
5954022 Apoptotic execution phase

Domain Information

InterPro Annotations

Accession Description
IPR003130 Dynamin GTPase effector
IPR000375 Dynamin central domain
IPR022812 Dynamin superfamily
IPR001401 Dynamin, GTPase domain
IPR019762 Dynamin, GTPase region, conserved site
IPR020850 GTPase effector domain, GED
IPR027417 P-loop containing nucleoside triphosphate hydrolase

UniProt Annotations

Entry Information

Gene Name
dynamin 1-like
Protein Entry
DNM1L_RAT
UniProt ID
O35303
Species
Rat

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=6; Name=1; IsoId=O35303-1; Sequence=Displayed; Name=2; IsoId=O35303-2; Sequence=VSP_013697, VSP_013701; Name=3; Synonyms=DLP1-37; IsoId=O35303-3; Sequence=VSP_013698, VSP_013699; Name=4; IsoId=O35303-4; Sequence=VSP_013696; Note=No experimental confirmation available.; Name=5; Synonyms=DLP1-11; IsoId=O35303-5; Sequence=VSP_013702; Name=6; IsoId=O35303-6; Sequence=VSP_013700; Note=No experimental confirmation available.;
Catalytic Activity GTP + H(2)O = GDP + phosphate.
Domain The GED domain folds back to interact, in cis, with the GTP-binding domain and middle domain, and interacts, in trans, with the GED domains of other DNM1L molecules, and is thus critical for activating GTPase activity and for DNM1L dimerization
Function Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane- associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage. Required for normal brain development, including that of cerebellum. Facilitates developmentally regulated apoptosis during neural tube formation. Required for a normal rate of cytochrome c release and caspase activation during apoptosis; this requirement may depend upon the cell type and the physiological apoptotic cues. Also required for mitochondrial fission during mitosis. Required for formation of endocytic vesicles. Proposed to regulate synaptic vesicle membrane dynamics through association with BCL2L1 isoform Bcl-X(L) which stimulates its GTPase activity in synaptic vesicles; the function may require its recruitment by MFF to clathrin-containing vesicles. Required for programmed necrosis execution. {ECO:0000269|PubMed:11553726, ECO:0000269|PubMed:12499366, ECO:0000269|PubMed:12861026, ECO:0000269|PubMed:17301055, ECO:0000269|PubMed:18250306, ECO:0000269|PubMed:23792689}.
Induction By bezafibrate
Interaction P53563-1:Bcl2l1; NbExp=2; IntAct=EBI-1767447, EBI-287204;
Ptm O-GlcNAcylation augments the level of the GTP-bound active form of DRP1 and induces translocation from the cytoplasm to mitochondria in cardiomyocytes. It also decreases phosphorylation at Ser-656
Ptm Phosphorylation/dephosphorylation events on two sites near the GED domain regulate mitochondrial fission. Phosphorylation on Ser-656 inhibits mitochondrial fission probably through preventing intramolecular interaction. Dephosphorylated on this site by PPP3CA which promotes mitochondrial fission. Phosphorylation on Ser-635 also promotes mitochondrial fission (By similarity)
Ptm S-nitrosylation increases DNM1L dimerization, mitochondrial fission and causes neuronal damage
Ptm Sumoylated on various lysine residues within the B domain, probably by MUL1. Sumoylation positively regulates mitochondrial fission. Desumoylated by SENP5 during G2/M transition of mitosis. Appears to be linked to its catalytic activity (By similarity)
Ptm Ubiquitinated by MARCH5
Similarity Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family
Similarity Contains 1 GED domain. {ECO:0000255|PROSITE- ProRule:PRU00720}.
Similarity Contains 1 dynamin-type G (guanine nucleotide-binding) domain
Subcellular Location Cytoplasm, cytosol . Golgi apparatus . Endomembrane system; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein. Peroxisome . Membrane, clathrin-coated pit. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Note=Mainly cytosolic. Translocated to the mitochondrial membrane through O-GlcNAcylation and interaction with FIS1. Colocalized with MARCH5 at mitochondrial membrane. Localizes to mitochondria at sites of division. Localizes to mitochondria following necrosis induction. Associated with peroxisomal membranes, partly recruited there by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear. In some cell types, localizes to the Golgi complex. Binds to phospholipid membranes (By similarity)
Subunit Homotetramer; dimerizes through the N-terminal GTP-middle region of one molecule binding to the GED domain of another DNM1L molecule. Oligomerizes in a GTP-dependent manner to form membrane- associated tubules with a spiral pattern. Can also oligomerize to form multimeric ring-like structures. Interacts with GSK3B and MARCH5. Interacts (via the GTPase and B domains) with UBE2I; the interaction promotes sumoylation of DNM1L, mainly in its B domain. Interacts with PPP3CA; the interaction dephosphorylates DNM1L and regulates its transition to mitochondria. Interacts with BCL2L1 isoform BCL-X(L) and CLTA; DNM1L and BCL2L1 isoform BCL-X(L) may form a complex in synaptic vesicles that also contains clathrin and MFF. Interacts with FIS1. Interacts with MIEF2 and MIEF1; this regulates GTP hydrolysis and DNM1L oligomerization. Interacts with PGAM5; this interaction leads to dephosphorylation at Ser-656 and activation of GTPase activity and eventually to mitochondria fragmentation. {ECO:0000269|PubMed:10679301, ECO:0000269|PubMed:12861026, ECO:0000269|PubMed:18250306, ECO:0000269|PubMed:23792689}.
Tissue Specificity Expressed in all tissues tested (at protein level). Longer isoforms are preferentially expressed in brain

Identical and Related Proteins

Unique RefSeq proteins for LMP012860 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
77917614 RefSeq NP_446107 716 dynamin-1-like protein

Identical Sequences to LMP012860 proteins

Reference Database Accession Length Protein Name
GI:77917614 GenBank AAH85843.1 716 Dynamin 1-like [Rattus norvegicus]

Related Sequences to LMP012860 proteins

Reference Database Accession Length Protein Name
GI:77917614 GenBank AAH85843.1 716 Dynamin 1-like [Rattus norvegicus]
GI:77917614 RefSeq XP_006248784.1 722 PREDICTED: dynamin-1-like protein isoform X16 [Rattus norvegicus]
GI:77917614 RefSeq XP_008767067.1 735 PREDICTED: dynamin-1-like protein isoform X12 [Rattus norvegicus]