Gene/Proteome Database (LMPD)
LMPD ID
LMP012867
Gene ID
Species
Rattus norvegicus (Rat)
Gene Name
phospholipase C, epsilon 1
Gene Symbol
Synonyms
Plce;
Alternate Names
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1; PLC-epsilon-1; phospholipase C epsilon; phospholipase C-epsilon-1; phosphoinositide phospholipase C-epsilon-1; phosphoinositide-specific phospholipase C epsilon-1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase epsilon-1;
Chromosome
1
Map Location
1q53
EC Number
3.1.4.11
Summary
one of four classes of phospholipase C; directly regulated by Ras [RGD, Feb 2006]
Orthologs
Proteins
| 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 | |
|---|---|
| Refseq ID | NP_446210 |
| Protein GI | 16758594 |
| UniProt ID | Q99P84 |
| mRNA ID | NM_053758 |
| Length | 2281 |
| MTSEEMAASFLIPVPQRKVASAQSVAEERGEKVSEAGIPKTRAGRQGGLTPRTISQRNEPEEESPRTDFSQVFSIARGELDSDENHNERCWEENVPGSTKNHAVNCNSLLQSHQHALPPSQLCEVCDSVTEEHLCLQPGIPSPLERKVFPGIELEMEDSPMDVSPLGNQPGIMESSGPHSDRNMAVFHFHYAGDRTMPGAFHTLSEKFILDDCANCVTLPGGQQNKNYMAYTCKLVELTRTCGSKNGQLKCDHCTSLRDEYLCFESSCRKAEALSSGGGFCEDGFTHGPSAKTFLNPLEEFSDNCEDVDDIFKGKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLAAWSYVDQKKAGLMWPCGNGMRPLSTVDVRQSGRQRLSEAQWCLIYSAVRREETEDTVGSLLHCSTQLPTPDTAHGRIGDGPCLKQCVRDSECEYRATLQRTSIAQYITGSLLEATTSLGARSSLLSSFGGSTGRIMLKERQPGTSMANSSPVPSSSAGISKELIDLQPLIQFPEEVASILTEQEQNIYRRVLPMDYLCFLTRDLSSPECQRSLPRLKACISESILMSQSGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSVEYKKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLLKLCPRYSSQEEALEFVADYSGQDNFLQRVGQNGLKNPEKELTVNSIFQIIRSCSRSLETEDEESASEGSGSRKNSLKDKTRWQFIIGDLLDSDNDIFEKSKECDPHGSEESQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWMKPPTASPAGARLKLGVLSNVAEPGKFPSLGNAGVSGLVEGILDLFSVKAVYMGHPGIDIHTVCVQNKLSSMLLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTTAVRKIRKFPDQRQQWLRKQYVSFYQEDGRYEGPTLAHAVELFGGRRWSTRNPSPGMSAKNAEKPNMQRNNTLGISTTKKKKKMLMRGESGEVTDDEMATRKAKMYRECRSRSGSDPQEANEQEDSEANVITNPPNPLHSRRAYSLTTAGSPNLATGMSSPISAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFIEFVELFKSFSIRSRKDLKDIFDIYSVPCNRSASESTPLYTNLTIEENTNDLQPDLDLLTRNVSDLGLFMKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPNISMCHQGLLSFEGFARFLMDKDNFASKNDESRENKKDLQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSIELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFITSDLPIIISIENHCSLPQQRKMAEIFKSVFGEKLVAKFLFETDFSDDPMLPSPDQLRRKVLLKNKKLKAHQTPVDILKQKAHQLASMQTQAFTGGNANPPPASNEEEEDEEDEYDYDYESLSDDNILEDRPENKSCADKLQFEYNEEVPKRIKKADNSSGNKGKVYDMELGEEFYLPQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNSSGSGRGKERKSRKSIFGNNPGRMSPGETASFNRTSGKSSCEGIRQIWEEPPLSPNTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLIQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKSCPMYQKFSPLERDLDAMDPATYSLTIISGQNVCPSNSTGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFRVHFEDLVFLRFAVVENNSSAITAQRIIPLKALKRGYRHLQLRNLHNEILEISSLFINSRRMEDNPSGSTRPASLMFNTEERKCSQTHKVTVHGVPGPEPFAVFTINEGTKAKQLLQQILAVDQDTKLTAADYFLMEEKHFISKEKNECRKQPFQRAVGPEEDIVQILNSWFPEEGYVGRIVLKPQQETLEEKNIVHDDREVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILNNPNPCDYVLLEEVMKDAPNKKSSTPKSSQRILLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKRRGISFASELKKLTKSTKQTRGLTSPPQLVASESVQSKEEKPMGALASGDTAGYQS | |
Gene Information
Entrez Gene ID
Gene Name
phospholipase C, epsilon 1
Gene Symbol
Species
Rattus norvegicus
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005737 | IDA:RGD | C | cytoplasm |
| GO:0005829 | ISS:UniProtKB | C | cytosol |
| GO:0005794 | IEA:UniProtKB-KW | C | Golgi apparatus |
| GO:0005886 | ISS:UniProtKB | C | plasma membrane |
| GO:0005509 | IEA:InterPro | F | calcium ion binding |
| GO:0019899 | ISS:UniProtKB | F | enzyme binding |
| GO:0005085 | IEA:UniProtKB-KW | F | guanyl-nucleotide exchange factor activity |
| GO:0004435 | ISS:UniProtKB | F | phosphatidylinositol phospholipase C activity |
| GO:0004629 | ISS:UniProtKB | F | phospholipase C activity |
| GO:0017016 | IDA:RGD | F | Ras GTPase binding |
| GO:0004871 | IEA:UniProtKB-KW | F | signal transducer activity |
| GO:0000187 | ISS:UniProtKB | P | activation of MAPK activity |
| GO:0007186 | IMP:RGD | P | G-protein coupled receptor signaling pathway |
| GO:0016042 | IEA:UniProtKB-KW | P | lipid catabolic process |
| GO:0007200 | ISS:UniProtKB | P | phospholipase C-activating G-protein coupled receptor signaling pathway |
| GO:0007265 | IDA:RGD | P | Ras protein signal transduction |
| GO:0008277 | ISS:UniProtKB | P | regulation of G-protein coupled receptor protein signaling pathway |
| GO:0045859 | ISS:UniProtKB | P | regulation of protein kinase activity |
| GO:0046578 | ISS:UniProtKB | P | regulation of Ras protein signal transduction |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ko04020 | Calcium signaling pathway |
| rno04020 | Calcium signaling pathway |
| ko04024 | cAMP signaling pathway |
| rno04024 | cAMP signaling pathway |
| ko00562 | Inositol phosphate metabolism |
| rno00562 | Inositol phosphate metabolism |
| M00130 | Inositol phosphate metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 |
| rno01100 | Metabolic pathways |
| ko04070 | Phosphatidylinositol signaling system |
| rno04070 | Phosphatidylinositol signaling system |
| ko05205 | Proteoglycans in cancer |
| rno05205 | Proteoglycans in cancer |
| ko04015 | Rap1 signaling pathway |
| rno04015 | Rap1 signaling pathway |
| rno04014 | Ras signaling pathway |
| rno04919 | Thyroid hormone signaling pathway |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR028398 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 |
| IPR000008 | C2 domain |
| IPR011992 | EF-hand domain pair |
| IPR001192 | Phosphoinositide phospholipase C family |
| IPR000909 | Phospholipase C, phosphatidylinositol-specific , X domain |
| IPR001711 | Phospholipase C, phosphatidylinositol-specific, Y domain |
| IPR015359 | Phospholipase C, phosphoinositol-specific, EF-hand-like |
| IPR017946 | PLC-like phosphodiesterase, TIM beta/alpha-barrel domain |
| IPR000159 | Ras-association |
| IPR023578 | Ras guanine nucleotide exchange factor domain |
| IPR001895 | Ras guanine-nucleotide exchange factors catalytic domain |
| IPR029071 | Ubiquitin-related domain |
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Biophysicochemical Properties | Kinetic parameters: KM=6.0 uM for PtdIns(4,5)P2 ; Vmax=10 umol/min/mg enzyme ; |
| Catalytic Activity | 1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol |
| Cofactor | Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence= ; |
| Domain | The Ras-associating domain 1 is degenerated and may not bind HRAS (By similarity). The Ras-associating domain 2 mediates interaction with GTP-bound HRAS, RAP1A and probably RAP2A and RAP2B and recruitment of HRAS to the cell membrane |
| Domain | The Ras-GEF domain has a GEF activity towards HRAS and RAP1A. Mediates activation of the mitogen-activated protein kinase pathway (By similarity) |
| Enzyme Regulation | Activated by the heterotrimeric G-protein subunits GNA12, GNA13 and GNB1-GNG2. Activated by HRAS, RAP1A, RHOA, RHOB, RHOC, RRAS and RRAS2. Activated by the G(s)-coupled GPCRs ADRB2, PTGER1 and CHRM3 through cyclic-AMP formation and RAP2B activation. Inhibited by G(i)-coupled GPCRs. {ECO:0000269|PubMed:11179219, ECO:0000269|PubMed:11641393, ECO:0000269|PubMed:11788587, ECO:0000269|PubMed:12900402, ECO:0000269|PubMed:15322077}. |
| Function | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. PLCE1 is a bifunctional enzyme which also regulates small GTPases of the Ras superfamily through its Ras guanine- exchange factor (RasGEF) activity. As an effector of heterotrimeric and small G-protein, it may play a role in cell survival, cell growth, actin organization and T-cell activation. {ECO:0000269|PubMed:12721365, ECO:0000269|PubMed:16314422, ECO:0000269|PubMed:17086182}. |
| Similarity | Contains 1 C2 domain. {ECO:0000255|PROSITE- ProRule:PRU00041}. |
| Similarity | Contains 1 PI-PLC X-box domain. {ECO:0000255|PROSITE- ProRule:PRU00270}. |
| Similarity | Contains 1 PI-PLC Y-box domain. {ECO:0000255|PROSITE- ProRule:PRU00271}. |
| Similarity | Contains 1 Ras-GEF domain. {ECO:0000255|PROSITE- ProRule:PRU00168}. |
| Similarity | Contains 2 Ras-associating domains |
| Subcellular Location | Cytoplasm, cytosol . Cell membrane {ECO:0000250}. Golgi apparatus membrane . Note=Recruited to plasma membrane by activated HRAS and RAP2. Recruited to perinuclear membrane by activated RAP1A. Associates with Golgi membranes (By similarity) |
| Subunit | Interacts with GTP-bound HRAS, RAP1A and probably with RAP2A, RAP2B and RRAS. Interacts with RHOA. Interacts with IQGAP1 (By similarity) |
| Tissue Specificity | Ubiquitously expressed. Detected in glomerular podocytes (at protein level). {ECO:0000269|PubMed:11179219, ECO:0000269|PubMed:17086182}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP012867 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 16758594 | RefSeq | NP_446210 | 2281 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 |
Identical Sequences to LMP012867 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:16758594 | EMBL | CBU87826.1 | 2281 | unnamed protein product [Rattus norvegicus] |
| GI:16758594 | EMBL | CBU96773.1 | 2281 | unnamed protein product [Rattus norvegicus] |
| GI:16758594 | GenBank | AGX54761.1 | 2281 | Sequence 9878 from patent US 8541208 |
| GI:16758594 | GenBank | AGX64201.1 | 2281 | Sequence 28512 from patent US 8541208 |
Related Sequences to LMP012867 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:16758594 | EMBL | CBU87826.1 | 2281 | unnamed protein product [Rattus norvegicus] |
| GI:16758594 | EMBL | CBU96773.1 | 2281 | unnamed protein product [Rattus norvegicus] |
| GI:16758594 | GenBank | AGX54761.1 | 2281 | Sequence 9878 from patent US 8541208 |