Gene/Proteome Database (LMPD)

LMPD ID
LMP012867
Gene ID
114633
Species
Rattus norvegicus (Rat)
Gene Name
phospholipase C, epsilon 1
Gene Symbol
Plce1
Synonyms
Plce;
Alternate Names
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1; PLC-epsilon-1; phospholipase C epsilon; phospholipase C-epsilon-1; phosphoinositide phospholipase C-epsilon-1; phosphoinositide-specific phospholipase C epsilon-1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase epsilon-1;
Chromosome
1
Map Location
1q53
EC Number
3.1.4.11
Summary
one of four classes of phospholipase C; directly regulated by Ras [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Plce1

Proteins

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
Refseq ID NP_446210
Protein GI 16758594
UniProt ID Q99P84
mRNA ID NM_053758
Length 2281
MTSEEMAASFLIPVPQRKVASAQSVAEERGEKVSEAGIPKTRAGRQGGLTPRTISQRNEPEEESPRTDFSQVFSIARGELDSDENHNERCWEENVPGSTKNHAVNCNSLLQSHQHALPPSQLCEVCDSVTEEHLCLQPGIPSPLERKVFPGIELEMEDSPMDVSPLGNQPGIMESSGPHSDRNMAVFHFHYAGDRTMPGAFHTLSEKFILDDCANCVTLPGGQQNKNYMAYTCKLVELTRTCGSKNGQLKCDHCTSLRDEYLCFESSCRKAEALSSGGGFCEDGFTHGPSAKTFLNPLEEFSDNCEDVDDIFKGKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLAAWSYVDQKKAGLMWPCGNGMRPLSTVDVRQSGRQRLSEAQWCLIYSAVRREETEDTVGSLLHCSTQLPTPDTAHGRIGDGPCLKQCVRDSECEYRATLQRTSIAQYITGSLLEATTSLGARSSLLSSFGGSTGRIMLKERQPGTSMANSSPVPSSSAGISKELIDLQPLIQFPEEVASILTEQEQNIYRRVLPMDYLCFLTRDLSSPECQRSLPRLKACISESILMSQSGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSVEYKKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLLKLCPRYSSQEEALEFVADYSGQDNFLQRVGQNGLKNPEKELTVNSIFQIIRSCSRSLETEDEESASEGSGSRKNSLKDKTRWQFIIGDLLDSDNDIFEKSKECDPHGSEESQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWMKPPTASPAGARLKLGVLSNVAEPGKFPSLGNAGVSGLVEGILDLFSVKAVYMGHPGIDIHTVCVQNKLSSMLLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTTAVRKIRKFPDQRQQWLRKQYVSFYQEDGRYEGPTLAHAVELFGGRRWSTRNPSPGMSAKNAEKPNMQRNNTLGISTTKKKKKMLMRGESGEVTDDEMATRKAKMYRECRSRSGSDPQEANEQEDSEANVITNPPNPLHSRRAYSLTTAGSPNLATGMSSPISAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFIEFVELFKSFSIRSRKDLKDIFDIYSVPCNRSASESTPLYTNLTIEENTNDLQPDLDLLTRNVSDLGLFMKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPNISMCHQGLLSFEGFARFLMDKDNFASKNDESRENKKDLQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSIELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFITSDLPIIISIENHCSLPQQRKMAEIFKSVFGEKLVAKFLFETDFSDDPMLPSPDQLRRKVLLKNKKLKAHQTPVDILKQKAHQLASMQTQAFTGGNANPPPASNEEEEDEEDEYDYDYESLSDDNILEDRPENKSCADKLQFEYNEEVPKRIKKADNSSGNKGKVYDMELGEEFYLPQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNSSGSGRGKERKSRKSIFGNNPGRMSPGETASFNRTSGKSSCEGIRQIWEEPPLSPNTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLIQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKSCPMYQKFSPLERDLDAMDPATYSLTIISGQNVCPSNSTGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFRVHFEDLVFLRFAVVENNSSAITAQRIIPLKALKRGYRHLQLRNLHNEILEISSLFINSRRMEDNPSGSTRPASLMFNTEERKCSQTHKVTVHGVPGPEPFAVFTINEGTKAKQLLQQILAVDQDTKLTAADYFLMEEKHFISKEKNECRKQPFQRAVGPEEDIVQILNSWFPEEGYVGRIVLKPQQETLEEKNIVHDDREVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILNNPNPCDYVLLEEVMKDAPNKKSSTPKSSQRILLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKRRGISFASELKKLTKSTKQTRGLTSPPQLVASESVQSKEEKPMGALASGDTAGYQS

Gene Information

Entrez Gene ID
114633
Gene Name
phospholipase C, epsilon 1
Gene Symbol
Plce1
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005794 IEA:UniProtKB-KW C Golgi apparatus
GO:0005737 IDA:RGD C cytoplasm
GO:0005829 ISS:UniProtKB C cytosol
GO:0005886 ISS:UniProtKB C plasma membrane
GO:0017016 IDA:RGD F Ras GTPase binding
GO:0005509 IEA:InterPro F calcium ion binding
GO:0019899 ISS:UniProtKB F enzyme binding
GO:0005085 IEA:UniProtKB-KW F guanyl-nucleotide exchange factor activity
GO:0004435 ISS:UniProtKB F phosphatidylinositol phospholipase C activity
GO:0004629 ISS:UniProtKB F phospholipase C activity
GO:0004871 IEA:UniProtKB-KW F signal transducer activity
GO:0007186 IMP:RGD P G-protein coupled receptor signaling pathway
GO:0007265 IDA:RGD P Ras protein signal transduction
GO:0000187 ISS:UniProtKB P activation of MAPK activity
GO:0016042 IEA:UniProtKB-KW P lipid catabolic process
GO:0007200 ISS:UniProtKB P phospholipase C-activating G-protein coupled receptor signaling pathway
GO:0008277 ISS:UniProtKB P regulation of G-protein coupled receptor protein signaling pathway
GO:0046578 ISS:UniProtKB P regulation of Ras protein signal transduction
GO:0045859 ISS:UniProtKB P regulation of protein kinase activity

KEGG Pathway Links

KEGG Pathway ID Description
ko04020 Calcium signaling pathway
rno04020 Calcium signaling pathway
ko00562 Inositol phosphate metabolism
rno00562 Inositol phosphate metabolism
M00130 Inositol phosphate metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4
rno01100 Metabolic pathways
ko04070 Phosphatidylinositol signaling system
rno04070 Phosphatidylinositol signaling system
ko05205 Proteoglycans in cancer
rno05205 Proteoglycans in cancer
ko04015 Rap1 signaling pathway
rno04015 Rap1 signaling pathway
rno04014 Ras signaling pathway
rno04919 Thyroid hormone signaling pathway
ko04024 cAMP signaling pathway
rno04024 cAMP signaling pathway

Domain Information

InterPro Annotations

Accession Description
IPR028398 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
IPR000008 C2 domain
IPR011992 EF-hand domain pair
IPR017946 PLC-like phosphodiesterase, TIM beta/alpha-barrel domain
IPR001192 Phosphoinositide phospholipase C family
IPR000909 Phospholipase C, phosphatidylinositol-specific , X domain
IPR001711 Phospholipase C, phosphatidylinositol-specific, Y domain
IPR015359 Phospholipase C, phosphoinositol-specific, EF-hand-like
IPR023578 Ras guanine nucleotide exchange factor domain
IPR001895 Ras guanine-nucleotide exchange factors catalytic domain
IPR000159 Ras-association
IPR029071 Ubiquitin-related domain

UniProt Annotations

Entry Information

Gene Name
phospholipase C, epsilon 1
Protein Entry
PLCE1_RAT
UniProt ID
Q99P84
Species
Rat

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=6.0 uM for PtdIns(4,5)P2 ; Vmax=10 umol/min/mg enzyme ;
Catalytic Activity 1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
Cofactor Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence= ;
Domain The Ras-GEF domain has a GEF activity towards HRAS and RAP1A. Mediates activation of the mitogen-activated protein kinase pathway (By similarity)
Domain The Ras-associating domain 1 is degenerated and may not bind HRAS (By similarity). The Ras-associating domain 2 mediates interaction with GTP-bound HRAS, RAP1A and probably RAP2A and RAP2B and recruitment of HRAS to the cell membrane
Enzyme Regulation Activated by the heterotrimeric G-protein subunits GNA12, GNA13 and GNB1-GNG2. Activated by HRAS, RAP1A, RHOA, RHOB, RHOC, RRAS and RRAS2. Activated by the G(s)-coupled GPCRs ADRB2, PTGER1 and CHRM3 through cyclic-AMP formation and RAP2B activation. Inhibited by G(i)-coupled GPCRs. {ECO:0000269|PubMed:11179219, ECO:0000269|PubMed:11641393, ECO:0000269|PubMed:11788587, ECO:0000269|PubMed:12900402, ECO:0000269|PubMed:15322077}.
Function The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. PLCE1 is a bifunctional enzyme which also regulates small GTPases of the Ras superfamily through its Ras guanine- exchange factor (RasGEF) activity. As an effector of heterotrimeric and small G-protein, it may play a role in cell survival, cell growth, actin organization and T-cell activation. {ECO:0000269|PubMed:12721365, ECO:0000269|PubMed:16314422, ECO:0000269|PubMed:17086182}.
Similarity Contains 1 C2 domain. {ECO:0000255|PROSITE- ProRule:PRU00041}.
Similarity Contains 1 PI-PLC X-box domain. {ECO:0000255|PROSITE- ProRule:PRU00270}.
Similarity Contains 1 PI-PLC Y-box domain. {ECO:0000255|PROSITE- ProRule:PRU00271}.
Similarity Contains 1 Ras-GEF domain. {ECO:0000255|PROSITE- ProRule:PRU00168}.
Similarity Contains 2 Ras-associating domains
Subcellular Location Cytoplasm, cytosol . Cell membrane {ECO:0000250}. Golgi apparatus membrane . Note=Recruited to plasma membrane by activated HRAS and RAP2. Recruited to perinuclear membrane by activated RAP1A. Associates with Golgi membranes (By similarity)
Subunit Interacts with GTP-bound HRAS, RAP1A and probably with RAP2A, RAP2B and RRAS. Interacts with RHOA. Interacts with IQGAP1 (By similarity)
Tissue Specificity Ubiquitously expressed. Detected in glomerular podocytes (at protein level). {ECO:0000269|PubMed:11179219, ECO:0000269|PubMed:17086182}.

Identical and Related Proteins

Unique RefSeq proteins for LMP012867 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16758594 RefSeq NP_446210 2281 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1

Identical Sequences to LMP012867 proteins

Reference Database Accession Length Protein Name
GI:16758594 EMBL CBU87826.1 2281 unnamed protein product [Rattus norvegicus]
GI:16758594 EMBL CBU96773.1 2281 unnamed protein product [Rattus norvegicus]
GI:16758594 GenBank AGX54761.1 2281 Sequence 9878 from patent US 8541208
GI:16758594 GenBank AGX64201.1 2281 Sequence 28512 from patent US 8541208

Related Sequences to LMP012867 proteins

Reference Database Accession Length Protein Name
GI:16758594 EMBL CBU87826.1 2281 unnamed protein product [Rattus norvegicus]
GI:16758594 EMBL CBU96773.1 2281 unnamed protein product [Rattus norvegicus]
GI:16758594 GenBank AGX54761.1 2281 Sequence 9878 from patent US 8541208