Gene/Proteome Database (LMPD)
LMPD ID
LMP012876
Gene ID
Species
Rattus norvegicus (Rat)
Gene Name
valosin-containing protein
Gene Symbol
Alternate Names
transitional endoplasmic reticulum ATPase; TER ATPase; 15S Mg(2+)-ATPase p97 subunit;
Chromosome
5
Map Location
5q22
EC Number
3.6.4.6
Summary
ATP binding protein involved in vesicle transport and fusion; important for proper functioning of the endoplasmic reticulum [RGD, Feb 2006]
Orthologs
Proteins
transitional endoplasmic reticulum ATPase | |
---|---|
Refseq ID | NP_446316 |
Protein GI | 17865351 |
UniProt ID | P46462 |
mRNA ID | NM_053864 |
Length | 806 |
MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGNVYTEDNDDDLYG |
Gene Information
Entrez Gene ID
Gene Name
valosin-containing protein
Gene Symbol
Species
Rattus norvegicus
Gene Ontology (GO Annotations)
GO ID | Source | Type | Description |
---|---|---|---|
GO:0000836 | IEA:Ensembl | C | Hrd1p ubiquitin ligase complex |
GO:0005829 | IDA:MGI | C | cytosol |
GO:0005783 | IDA:RGD | C | endoplasmic reticulum |
GO:0070062 | IEA:Ensembl | C | extracellular vesicular exosome |
GO:0043231 | IDA:RGD | C | intracellular membrane-bounded organelle |
GO:0005811 | IEA:Ensembl | C | lipid particle |
GO:0005634 | IEA:UniProtKB-KW | C | nucleus |
GO:0048471 | IEA:Ensembl | C | perinuclear region of cytoplasm |
GO:0000502 | IEA:Ensembl | C | proteasome complex |
GO:0035861 | ISS:UniProtKB | C | site of double-strand break |
GO:0043531 | IMP:RGD | F | ADP binding |
GO:0005524 | IDA:BHF-UCL | F | ATP binding |
GO:0016887 | IDA:RGD | F | ATPase activity |
GO:0035800 | IEA:Ensembl | F | deubiquitinase activator activity |
GO:0042802 | IDA:BHF-UCL | F | identical protein binding |
GO:0008289 | IEA:UniProtKB-KW | F | lipid binding |
GO:0044822 | IEA:Ensembl | F | poly(A) RNA binding |
GO:0032403 | IPI:RGD | F | protein complex binding |
GO:0005102 | IDA:RGD | F | receptor binding |
GO:0006200 | IDA:GOC | P | ATP catabolic process |
GO:0006888 | IMP:RGD | P | ER to Golgi vesicle-mediated transport |
GO:0030433 | ISS:UniProtKB | P | ER-associated ubiquitin-dependent protein catabolic process |
GO:0006919 | IEA:Ensembl | P | activation of cysteine-type endopeptidase activity involved in apoptotic process |
GO:0070842 | IEA:Ensembl | P | aggresome assembly |
GO:0006974 | ISS:UniProtKB | P | cellular response to DNA damage stimulus |
GO:0006302 | ISS:UniProtKB | P | double-strand break repair |
GO:1903007 | IEA:Ensembl | P | positive regulation of Lys63-specific deubiquitinase activity |
GO:0032436 | IMP:RGD | P | positive regulation of proteasomal ubiquitin-dependent protein catabolic process |
GO:1903006 | IEA:Ensembl | P | positive regulation of protein K63-linked deubiquitination |
GO:0031334 | IEA:Ensembl | P | positive regulation of protein complex assembly |
GO:0018279 | ISS:UniProtKB | P | protein N-linked glycosylation via asparagine |
GO:0034214 | IDA:RGD | P | protein hexamerization |
GO:0051260 | IDA:RGD | P | protein homooligomerization |
GO:0016567 | ISS:UniProtKB | P | protein ubiquitination |
GO:0030970 | IEA:Ensembl | P | retrograde protein transport, ER to cytosol |
GO:0019985 | ISS:UniProtKB | P | translesion synthesis |
KEGG Pathway Links
KEGG Pathway ID | Description |
---|---|
M00403 | HRD1/SEL1 ERAD complex |
ko05134 | Legionellosis |
rno05134 | Legionellosis |
ko04141 | Protein processing in endoplasmic reticulum |
rno04141 | Protein processing in endoplasmic reticulum |
M00400 | p97-Ufd1-Npl4 complex |
REACTOME Pathway Links
REACTOME Pathway ID | Description |
---|---|
5954621 | Cellular response to heat stress |
5953238 | Cellular responses to stress |
5953253 | Disease |
5954622 | HSF1 activation |
5954643 | Hedgehog ligand biogenesis |
5954646 | Hh ligand biogenesis disease |
5954645 | Processing-defective Hh variants abrogate ligand secretion |
5953381 | Signal Transduction |
5954644 | Signaling by Hedgehog |
Domain Information
InterPro Annotations
Accession | Description |
---|---|
IPR003593 | AAA+ ATPase domain |
IPR005938 | AAA_ATPase_CDC48 |
IPR003960 | ATPase, AAA-type, conserved site |
IPR003959 | ATPase, AAA-type, core |
IPR009010 | Aspartate decarboxylase-like domain |
IPR029067 | CDC48 domain 2-like |
IPR003338 | CDC4_N-term_subdom |
IPR004201 | Cdc48_dom2 |
IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
IPR015415 | Vps4_C |
UniProt Annotations
Entry Information
Comments
Comment Type | Description |
---|---|
Catalytic Activity | ATP + H(2)O = ADP + phosphate. |
Function | Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites. Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage. Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. {ECO:0000250|UniProtKB:P55072, ECO:0000269|PubMed:10930451, ECO:0000269|PubMed:12411482}. |
Interaction | O35987:Nsfl1c; NbExp=10; IntAct=EBI-399011, EBI-1993760; |
Ptm | ISGylated |
Ptm | Methylation at Lys-315 catalyzed by VCPKMT is increased in the presence of ASPSCR1. Lys-315 methylation may decrease ATPase activity (By similarity) |
Ptm | Phosphorylated by tyrosine kinases in response to T-cell antigen receptor activation. Phosphorylated in mitotic cells |
Similarity | Belongs to the AAA ATPase family |
Subcellular Location | Cytoplasm, cytosol . Nucleus . Endoplasmic reticulum . Note=Recruited to the cytoplasmic surface of the endoplasmic reticulum via interaction with AMFR/gp78. Following DNA double-strand breaks, recruited to the sites of damage. Recruited to stalled replication forks via interaction with SPRTN (By similarity) |
Subunit | Homohexamer. Forms a ring-shaped particle of 12.5 nm diameter, that displays 6-fold radial symmetry. Interacts with NSFL1C-like protein p37; the complex has membrane fusion activity and is required for Golgi and endoplasmic reticulum biogenesis. Interacts with RHBDD1 (via C-terminal domain). Interacts with VIMP/SELS and SYVN1, as well as with DERL1, DERL2 and DERL3; which probably transfer misfolded proteins from the ER to VCP. Interacts with SVIP. Component of a complex required to couple retrotranslocation, ubiquitination and deglycosylation composed of NGLY1, SAKS1, AMFR, VCP and RAD23B. Directly interacts with UBXD2 and RNF19A. Interacts with CASR. Interacts with UBXN6, UBE4B and YOD1. Interacts with clathrin. Interacts with RNF103. Interacts with TRIM13 and TRIM21. Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of the endoplasmic reticulum- associated degradation (ERAD) of HMGCR. Interacts directly with AMFR/gp78 (via its VIM). Interacts with SPRTN; leading to recruitment to stalled replication forks. Part of a ternary complex containing STX5A, NSFL1C and VCP. NSFL1C forms a homotrimer that binds to one end of a VCP homohexamer. The complex binds to membranes enriched in phosphatidylethanolamine-containing lipids and promotes Golgi membrane fusion. Binds to a heterodimer of NPLOC4 and UFD1L, binding to this heterodimer inhibits Golgi- membrane fusion. Interaction with VCIP135 leads to dissociation of the complex via ATP hydrolysis by VCP. Part of a ternary complex containing NPLOC4, UFD1L and VCP. Part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, STT3A, VCP AND VIMP. Interacts with KIAA0196. Interacts with UBOX5. {ECO:0000250|UniProtKB:P55072, ECO:0000250|UniProtKB:Q01853, ECO:0000269|PubMed:10811609, ECO:0000269|PubMed:12411482, ECO:0000269|PubMed:12473691, ECO:0000269|PubMed:9506515}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP012876 (as displayed in Record Overview)
Protein GI | Database | Accession | Length | Protein Name |
---|---|---|---|---|
17865351 | RefSeq | NP_446316 | 806 | transitional endoplasmic reticulum ATPase |
Identical Sequences to LMP012876 proteins
Reference | Database | Accession | Length | Protein Name |
---|---|---|---|---|
GI:17865351 | GenBank | AAH60518.1 | 806 | Valosin-containing protein [Rattus norvegicus] |
GI:17865351 | GenBank | EDL98717.1 | 806 | valosin-containing protein, isoform CRA_b [Rattus norvegicus] |
GI:17865351 | GenBank | ACN00833.1 | 806 | Sequence 18 from patent US 7479369 |
GI:17865351 | SwissProt | P46462.3 | 806 | RecName: Full=Transitional endoplasmic reticulum ATPase; Short=TER ATPase; AltName: Full=15S Mg(2+)-ATPase p97 subunit; AltName: Full=Valosin-containing protein; Short=VCP [Rattus norvegicus] |
Related Sequences to LMP012876 proteins
Reference | Database | Accession | Length | Protein Name |
---|---|---|---|---|
GI:17865351 | GenBank | AAC52154.1 | 806 | transitional endoplasmic reticulum ATPase [Rattus norvegicus] |
GI:17865351 | GenBank | EDL98717.1 | 806 | valosin-containing protein, isoform CRA_b [Rattus norvegicus] |
GI:17865351 | PRF | - | 806 | transitional endoplasmic reticulum ATPase [Rattus norvegicus] |