Gene/Proteome Database (LMPD)

LMPD ID
LMP012876
Gene ID
116643
Species
Rattus norvegicus (Rat)
Gene Name
valosin-containing protein
Gene Symbol
Vcp
Alternate Names
transitional endoplasmic reticulum ATPase; TER ATPase; 15S Mg(2+)-ATPase p97 subunit;
Chromosome
5
Map Location
5q22
EC Number
3.6.4.6
Summary
ATP binding protein involved in vesicle transport and fusion; important for proper functioning of the endoplasmic reticulum [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Vcp

Proteins

transitional endoplasmic reticulum ATPase
Refseq ID NP_446316
Protein GI 17865351
UniProt ID P46462
mRNA ID NM_053864
Length 806
MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGNVYTEDNDDDLYG

Gene Information

Entrez Gene ID
116643
Gene Name
valosin-containing protein
Gene Symbol
Vcp
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 IDA:MGI C cytosol
GO:0005783 IDA:RGD C endoplasmic reticulum
GO:0070062 IEA:Ensembl C extracellular vesicular exosome
GO:0000836 IEA:Ensembl C Hrd1p ubiquitin ligase complex
GO:0043231 IDA:RGD C intracellular membrane-bounded organelle
GO:0005811 IEA:Ensembl C lipid particle
GO:0005634 IEA:UniProtKB-KW C nucleus
GO:0048471 IEA:Ensembl C perinuclear region of cytoplasm
GO:0000502 IEA:Ensembl C proteasome complex
GO:0035861 ISS:UniProtKB C site of double-strand break
GO:0043531 IMP:RGD F ADP binding
GO:0016887 IDA:RGD F ATPase activity
GO:0005524 IDA:BHF-UCL F ATP binding
GO:0035800 IEA:Ensembl F deubiquitinase activator activity
GO:0042802 IDA:BHF-UCL F identical protein binding
GO:0008289 IEA:UniProtKB-KW F lipid binding
GO:0044822 IEA:Ensembl F poly(A) RNA binding
GO:0032403 IPI:RGD F protein complex binding
GO:0005102 IDA:RGD F receptor binding
GO:0006919 IEA:Ensembl P activation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0070842 IEA:Ensembl P aggresome assembly
GO:0006200 IDA:GOC P ATP catabolic process
GO:0006974 ISS:UniProtKB P cellular response to DNA damage stimulus
GO:0006302 ISS:UniProtKB P double-strand break repair
GO:0030433 ISS:UniProtKB P ER-associated ubiquitin-dependent protein catabolic process
GO:0006888 IMP:RGD P ER to Golgi vesicle-mediated transport
GO:1903007 IEA:Ensembl P positive regulation of Lys63-specific deubiquitinase activity
GO:0032436 IMP:RGD P positive regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0031334 IEA:Ensembl P positive regulation of protein complex assembly
GO:1903006 IEA:Ensembl P positive regulation of protein K63-linked deubiquitination
GO:0034214 IDA:RGD P protein hexamerization
GO:0051260 IDA:RGD P protein homooligomerization
GO:0018279 ISS:UniProtKB P protein N-linked glycosylation via asparagine
GO:0016567 ISS:UniProtKB P protein ubiquitination
GO:0030970 IEA:Ensembl P retrograde protein transport, ER to cytosol
GO:0019985 ISS:UniProtKB P translesion synthesis

KEGG Pathway Links

KEGG Pathway ID Description
M00403 HRD1/SEL1 ERAD complex
ko05134 Legionellosis
rno05134 Legionellosis
M00400 p97-Ufd1-Npl4 complex
ko04141 Protein processing in endoplasmic reticulum
rno04141 Protein processing in endoplasmic reticulum

REACTOME Pathway Links

REACTOME Pathway ID Description
5953238 Cellular responses to stress
5954621 Cellular response to heat stress
5953253 Disease
5954643 Hedgehog ligand biogenesis
5954646 Hh ligand biogenesis disease
5954622 HSF1 activation
5954645 Processing-defective Hh variants abrogate ligand secretion
5954644 Signaling by Hedgehog
5953381 Signal Transduction

Domain Information

InterPro Annotations

Accession Description
IPR005938 AAA_ATPase_CDC48
IPR003593 AAA+ ATPase domain
IPR009010 Aspartate decarboxylase-like domain
IPR003960 ATPase, AAA-type, conserved site
IPR003959 ATPase, AAA-type, core
IPR004201 Cdc48_dom2
IPR029067 CDC48 domain 2-like
IPR003338 CDC4_N-term_subdom
IPR027417 P-loop containing nucleoside triphosphate hydrolase
IPR015415 Vps4_C

UniProt Annotations

Entry Information

Gene Name
valosin-containing protein
Protein Entry
TERA_RAT
UniProt ID
P46462
Species
Rat

Comments

Comment Type Description
Catalytic Activity ATP + H(2)O = ADP + phosphate.
Function Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites. Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage. Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. {ECO:0000250|UniProtKB:P55072, ECO:0000269|PubMed:10930451, ECO:0000269|PubMed:12411482}.
Interaction O35987:Nsfl1c; NbExp=10; IntAct=EBI-399011, EBI-1993760;
Ptm ISGylated
Ptm Methylation at Lys-315 catalyzed by VCPKMT is increased in the presence of ASPSCR1. Lys-315 methylation may decrease ATPase activity (By similarity)
Ptm Phosphorylated by tyrosine kinases in response to T-cell antigen receptor activation. Phosphorylated in mitotic cells
Similarity Belongs to the AAA ATPase family
Subcellular Location Cytoplasm, cytosol . Nucleus . Endoplasmic reticulum . Note=Recruited to the cytoplasmic surface of the endoplasmic reticulum via interaction with AMFR/gp78. Following DNA double-strand breaks, recruited to the sites of damage. Recruited to stalled replication forks via interaction with SPRTN (By similarity)
Subunit Homohexamer. Forms a ring-shaped particle of 12.5 nm diameter, that displays 6-fold radial symmetry. Interacts with NSFL1C-like protein p37; the complex has membrane fusion activity and is required for Golgi and endoplasmic reticulum biogenesis. Interacts with RHBDD1 (via C-terminal domain). Interacts with VIMP/SELS and SYVN1, as well as with DERL1, DERL2 and DERL3; which probably transfer misfolded proteins from the ER to VCP. Interacts with SVIP. Component of a complex required to couple retrotranslocation, ubiquitination and deglycosylation composed of NGLY1, SAKS1, AMFR, VCP and RAD23B. Directly interacts with UBXD2 and RNF19A. Interacts with CASR. Interacts with UBXN6, UBE4B and YOD1. Interacts with clathrin. Interacts with RNF103. Interacts with TRIM13 and TRIM21. Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of the endoplasmic reticulum- associated degradation (ERAD) of HMGCR. Interacts directly with AMFR/gp78 (via its VIM). Interacts with SPRTN; leading to recruitment to stalled replication forks. Part of a ternary complex containing STX5A, NSFL1C and VCP. NSFL1C forms a homotrimer that binds to one end of a VCP homohexamer. The complex binds to membranes enriched in phosphatidylethanolamine-containing lipids and promotes Golgi membrane fusion. Binds to a heterodimer of NPLOC4 and UFD1L, binding to this heterodimer inhibits Golgi- membrane fusion. Interaction with VCIP135 leads to dissociation of the complex via ATP hydrolysis by VCP. Part of a ternary complex containing NPLOC4, UFD1L and VCP. Part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, STT3A, VCP AND VIMP. Interacts with KIAA0196. Interacts with UBOX5. {ECO:0000250|UniProtKB:P55072, ECO:0000250|UniProtKB:Q01853, ECO:0000269|PubMed:10811609, ECO:0000269|PubMed:12411482, ECO:0000269|PubMed:12473691, ECO:0000269|PubMed:9506515}.

Identical and Related Proteins

Unique RefSeq proteins for LMP012876 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
17865351 RefSeq NP_446316 806 transitional endoplasmic reticulum ATPase

Identical Sequences to LMP012876 proteins

Reference Database Accession Length Protein Name
GI:17865351 GenBank AAH60518.1 806 Valosin-containing protein [Rattus norvegicus]
GI:17865351 GenBank EDL98717.1 806 valosin-containing protein, isoform CRA_b [Rattus norvegicus]
GI:17865351 GenBank ACN00833.1 806 Sequence 18 from patent US 7479369
GI:17865351 SwissProt P46462.3 806 RecName: Full=Transitional endoplasmic reticulum ATPase; Short=TER ATPase; AltName: Full=15S Mg(2+)-ATPase p97 subunit; AltName: Full=Valosin-containing protein; Short=VCP [Rattus norvegicus]

Related Sequences to LMP012876 proteins

Reference Database Accession Length Protein Name
GI:17865351 GenBank AAC52154.1 806 transitional endoplasmic reticulum ATPase [Rattus norvegicus]
GI:17865351 GenBank EDL98717.1 806 valosin-containing protein, isoform CRA_b [Rattus norvegicus]
GI:17865351 PRF - 806 transitional endoplasmic reticulum ATPase [Rattus norvegicus]