Gene/Proteome Database (LMPD)

LMPD ID
LMP012907
Gene ID
140693
Species
Rattus norvegicus (Rat)
Gene Name
phospholipase C, delta 4
Gene Symbol
Plcd4
Alternate Names
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4; PLC-delta-4; phospholipase C-delta-4; phosphoinositide phospholipase C delta-4; phosphoinositide phospholipase C-delta-4; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-4;
Chromosome
9
Map Location
9q33
EC Number
3.1.4.11
Summary
mediates signal transduction by generating two second messenger molecules, inositol 1,4,5-trisphosphate (IP) and diacylglycerol, from phosphatidylinositol 4,5-bisphosphate (PIP) [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Plcd4

Proteins

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4
Refseq ID NP_542419
Protein GI 18093100
UniProt ID Q62711
mRNA ID NM_080688
Length 772
MASQIQKLLTTNQDLLLMQKGTMMRKVRTKSWKKLRYFRLQDDGMTVWHGRHLESISKPTFSISDVERIRKGQDSELLRYLVEEFPLEQGFTIVFNGRRPNLDLVANSVEEAQTWMRGLQLLVDLVARMNYQEQLDQMLREWFQQADRNQDSRMSFREAQRLLLLMNVEMDEEYAFSLFQEADVSQSNTLDSEEFVQFYKALTKRTEIEELFENFSSDKQKLTLLEFVDFLREEQKESDHSSDLALKLIDRYEPSENGRLLRVLSKDGFLSYLCSADGNIFNPDCLPIYQDMTQPLSHYYINSSHNTYLLGDQFCGQSSVEGYIRALKRGCRCVEVDTWDGPDGEPVVYHGRTLTSRILFKDVLATLAQYAFQSSDYPLILSLDNHCTWEQQKTMAHHLIAILGEQLLSTTLEEQLIDIMPSPEQLRGKILVKGKKLRTIEVVESDKEEEELEKDEGSDLDPASAELDMQSQPESQEQASGNKSKNKKKFLLQSSTTILCPDLSALVVYLRTAPFCSFTHSKENYHIYDISSFSESKAKNLIREAGNEFVQHNARQLCRVYPSGLRTDSSNYNPQEHWNVGCQMVAMNMQTAGSAMDICDGLFRQNGGSGYVLKPEFLRDTQSSFNPMKPVSLYKAQILVVQVISGQRLPKVDKTKETTVVDPLVRVELYGVPEDTKQQETSYVENNGINPYWGETFYFQIQVPELAMLRFVVKDYSRTSRNNFIGQYTLPWTCMKHGYRHVSLLSKDGTSLHPASIFVYTCMQEDLDMDEP

Gene Information

Entrez Gene ID
140693
Gene Name
phospholipase C, delta 4
Gene Symbol
Plcd4
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0016020 IEA:UniProtKB-KW C membrane
GO:0005634 IEA:UniProtKB-KW C nucleus
GO:0005509 IEA:InterPro F calcium ion binding
GO:0004435 IDA:RGD F phosphatidylinositol phospholipase C activity
GO:0004871 IEA:UniProtKB-KW F signal transducer activity
GO:0007340 IEA:InterPro P acrosome reaction
GO:0035556 TAS:RGD P intracellular signal transduction
GO:0016042 IEA:UniProtKB-KW P lipid catabolic process
GO:0046488 IEA:InterPro P phosphatidylinositol metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
ko04020 Calcium signaling pathway
rno04020 Calcium signaling pathway
ko00562 Inositol phosphate metabolism
rno00562 Inositol phosphate metabolism
M00130 Inositol phosphate metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4
rno01100 Metabolic pathways
ko04070 Phosphatidylinositol signaling system
rno04070 Phosphatidylinositol signaling system
rno04919 Thyroid hormone signaling pathway

Domain Information

InterPro Annotations

Accession Description
IPR028387 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4
IPR000008 C2 domain
IPR018247 EF-Hand 1, calcium-binding site
IPR002048 EF-hand domain
IPR011992 EF-hand domain pair
IPR001192 Phosphoinositide phospholipase C family
IPR000909 Phospholipase C, phosphatidylinositol-specific , X domain
IPR001711 Phospholipase C, phosphatidylinositol-specific, Y domain
IPR015359 Phospholipase C, phosphoinositol-specific, EF-hand-like
IPR017946 PLC-like phosphodiesterase, TIM beta/alpha-barrel domain
IPR001849 Pleckstrin homology domain
IPR011993 Pleckstrin homology-like domain

UniProt Annotations

Entry Information

Gene Name
phospholipase C, delta 4
Protein Entry
PLCD4_RAT
UniProt ID
Q62711
Species
Rat

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=4; Name=1; IsoId=Q62711-1; Sequence=Displayed; Name=2; Synonyms=ALT I; IsoId=Q62711-2; Sequence=VSP_028508; Name=3; Synonyms=ALT II; IsoId=Q62711-3; Sequence=VSP_028509; Name=4; Synonyms=ALT III; IsoId=Q62711-4; Sequence=VSP_028507; Note=Inactive.;
Catalytic Activity 1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol. {ECO:0000269|PubMed:8550568, ECO:0000269|PubMed:8550586}.
Cofactor Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence= ; Note=Binds 3 Ca(2+) ions per subunit. Two of the Ca(2+) ions are bound to the C2 domain. ;
Developmental Stage Increases at the transition from G1- to S- phase, and the continues to the end of M-phase. Almost disappears when cells reenter the next G1-phase
Domain The C2 domain mediates pre-localization to the membrane prior to Ca(2+) import and non-selective Ca(2+)-mediated targeting to various cellular membranes.
Domain The PDZ-binding motif mediates the interaction with GRIP1
Domain The PH domain is not a critical determinant of the membrane localization. The PH domain of isoform 4 is necessary and sufficient to inhibit enzyme activity of other PLC-delta enzymes.
Function Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca(2+) mobilization in the zona pellucida- induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression up-regulates the Erk signaling pathway and proliferation. Isoform 4 has no enzyme activity and acts as a negative regulator of phospholipases C, with a preference for PLC- delta family
Induction By serum treatment
Similarity Contains 1 C2 domain. {ECO:0000255|PROSITE- ProRule:PRU00041}.
Similarity Contains 1 PH domain. {ECO:0000255|PROSITE- ProRule:PRU00145}.
Similarity Contains 1 PI-PLC X-box domain. {ECO:0000255|PROSITE- ProRule:PRU00270}.
Similarity Contains 1 PI-PLC Y-box domain. {ECO:0000255|PROSITE- ProRule:PRU00271}.
Similarity Contains 3 EF-hand domains. {ECO:0000255|PROSITE- ProRule:PRU00448}.
Subcellular Location Endomembrane system; Peripheral membrane protein. Nucleus. Cytoplasm. Endoplasmic reticulum. Note=Localizes primarily to intracellular membranes mostly to the endoplasmic reticulum.
Subunit Interacts with GRIP1
Tissue Specificity Present at high level in testis. Also present in brain > skeletal muscle > thyroid gland > stomach > thymus > aorta > heart (at protein level). Highly expressed in regenerating liver. Isoform 4 is weakly expressed compared to other isoforms but is expressed at high level in some neural cells

Identical and Related Proteins

Unique RefSeq proteins for LMP012907 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
18093100 RefSeq NP_542419 772 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4

Identical Sequences to LMP012907 proteins

Reference Database Accession Length Protein Name
GI:18093100 EMBL CBU87795.1 772 unnamed protein product [Rattus norvegicus]
GI:18093100 EMBL CBU96742.1 772 unnamed protein product [Rattus norvegicus]
GI:18093100 GenBank AGX54730.1 772 Sequence 9816 from patent US 8541208
GI:18093100 GenBank AGX64170.1 772 Sequence 28450 from patent US 8541208

Related Sequences to LMP012907 proteins

Reference Database Accession Length Protein Name
GI:18093100 EMBL CBU87795.1 772 unnamed protein product [Rattus norvegicus]
GI:18093100 EMBL CBU96742.1 772 unnamed protein product [Rattus norvegicus]
GI:18093100 SwissProt Q62711.1 772 RecName: Full=1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4; AltName: Full=Phosphoinositide phospholipase C-delta-4; AltName: Full=Phospholipase C-delta-4; Short=PLC-delta-4 [Rattus norvegicus]