Gene/Proteome Database (LMPD)

LMPD ID
LMP012922
Gene ID
170670
Species
Rattus norvegicus (Rat)
Gene Name
hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), alpha subunit
Gene Symbol
Hadha
Synonyms
RGD1560655;
Alternate Names
trifunctional enzyme subunit alpha, mitochondrial; TP-alpha; hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit;
Chromosome
6
Map Location
6q14
EC Number
4.2.1.17
Summary
alpha subunit of the liver mitochondrial fatty acid oxidation multienzyme complex [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Hadha

Proteins

trifunctional enzyme subunit alpha, mitochondrial precursor
Refseq ID NP_570839
Protein GI 148747393
UniProt ID Q64428
mRNA ID NM_130826
Length 763
MVASRAIGSLSRFSAFRILRSRGCICHSFTTSSALLSRTHINYGVKGDVAVIRINSPNSKVNTLNKEVQSEFVEVMNEIWANDQIRSAVLISSKPGCFVAGADINMLASCTTPQEAARISQEGQKMFEKLEKSPKPVVAAISGSCLGGGLELAIACQYRIATKDRKTVLGVPEVLLGILPGAGGTQRLPKMVGVPAAFDMMLTGRNIRADRAKKMGLVDQLVDPLGPGIKSPEERTIEYLEEVAVNFAKGLADRKVSAKQSKGLMEKLTSYAMTIPFVRQQVYKTVEEKVKKQTKGLYPAPLKIIDAVKTGLEQGNDAGYLAESEKFGELALTKESKALMGLYNGQVLCKKNKFGAPQKTVQQLAILGAGLMGAGIAQVSVDKGLKTLLKDTTVTGLGRGQQQVFKGLNDKVKKKALTSFERDSIFSNLIGQLDYKGFEKADMVIEAVFEDLAVKHKVLKEVESVTPEHCIFASNTSALPINQIAAVSQRPEKVIGMHYFSPVDKMQLLEIITTDKTSKDTTASAVAVGLKQGKVIIVVKDGPGFYTTRCLAPMMSEVIRILQEGVDPKKLDALTTGFGFPVGAATLADEVGIDVAQHVAEDLGKAFGERFGGGSVELLKLMVSKGFLGRKSGKGFYIYQSGSKNKNLNSEIDNILVNLRLPAKPEVSSDEDIQYRVITRFVNEAVLCLQEGILATPEEGDIGAVFGLGFPPCLGGPFRFVDLYGAQKVVDRLRKYESAYGTQFTPCQLLRDLANNSSKKFYQ
transit_peptide: 1..36 inference: non-experimental evidence, no additional details recorded note: Mitochondrion (Potential); propagated from UniProtKB/Swiss-Prot (Q64428.2) calculated_mol_wt: 3904 peptide sequence: MVASRAIGSLSRFSAFRILRSRGCICHSFTTSSALL mat_peptide: 37..763 product: Trifunctional enzyme subunit alpha, mitochondrial experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (Q64428.2) calculated_mol_wt: 78780 peptide sequence: SRTHINYGVKGDVAVIRINSPNSKVNTLNKEVQSEFVEVMNEIWANDQIRSAVLISSKPGCFVAGADINMLASCTTPQEAARISQEGQKMFEKLEKSPKPVVAAISGSCLGGGLELAIACQYRIATKDRKTVLGVPEVLLGILPGAGGTQRLPKMVGVPAAFDMMLTGRNIRADRAKKMGLVDQLVDPLGPGIKSPEERTIEYLEEVAVNFAKGLADRKVSAKQSKGLMEKLTSYAMTIPFVRQQVYKTVEEKVKKQTKGLYPAPLKIIDAVKTGLEQGNDAGYLAESEKFGELALTKESKALMGLYNGQVLCKKNKFGAPQKTVQQLAILGAGLMGAGIAQVSVDKGLKTLLKDTTVTGLGRGQQQVFKGLNDKVKKKALTSFERDSIFSNLIGQLDYKGFEKADMVIEAVFEDLAVKHKVLKEVESVTPEHCIFASNTSALPINQIAAVSQRPEKVIGMHYFSPVDKMQLLEIITTDKTSKDTTASAVAVGLKQGKVIIVVKDGPGFYTTRCLAPMMSEVIRILQEGVDPKKLDALTTGFGFPVGAATLADEVGIDVAQHVAEDLGKAFGERFGGGSVELLKLMVSKGFLGRKSGKGFYIYQSGSKNKNLNSEIDNILVNLRLPAKPEVSSDEDIQYRVITRFVNEAVLCLQEGILATPEEGDIGAVFGLGFPPCLGGPFRFVDLYGAQKVVDRLRKYESAYGTQFTPCQLLRDLANNSSKKFYQ

Gene Information

Entrez Gene ID
170670
Gene Name
hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), alpha subunit
Gene Symbol
Hadha
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0016507 IDA:RGD C mitochondrial fatty acid beta-oxidation multienzyme complex
GO:0005743 IEA:Ensembl C mitochondrial inner membrane
GO:0042645 IEA:Ensembl C mitochondrial nucleoid
GO:0005739 IDA:RGD C mitochondrion
GO:0003857 IDA:RGD F 3-hydroxyacyl-CoA dehydrogenase activity
GO:0003988 IDA:RGD F acetyl-CoA C-acyltransferase activity
GO:0004300 IDA:RGD F enoyl-CoA hydratase activity
GO:0000062 IDA:RGD F fatty-acyl-CoA binding
GO:0016509 IDA:RGD F long-chain-3-hydroxyacyl-CoA dehydrogenase activity
GO:0016508 IDA:RGD F long-chain-enoyl-CoA hydratase activity
GO:0051287 IDA:RGD F NAD binding
GO:0032403 IMP:RGD F protein complex binding
GO:0006635 IDA:RGD P fatty acid beta-oxidation
GO:0042493 IDA:RGD P response to drug
GO:0032868 IEA:Ensembl P response to insulin

KEGG Pathway Links

KEGG Pathway ID Description
ko00410 beta-Alanine metabolism
rno00410 beta-Alanine metabolism
M00087 beta-Oxidation
ko01040 Biosynthesis of unsaturated fatty acids
rno01040 Biosynthesis of unsaturated fatty acids
ko00650 Butanoate metabolism
rno00650 Butanoate metabolism
ko01200 Carbon metabolism
rno01200 Carbon metabolism
M00085 Fatty acid biosynthesis, elongation, mitochondria
ko00071 Fatty acid degradation
rno00071 Fatty acid degradation
ko00062 Fatty acid elongation
rno00062 Fatty acid elongation
ko01212 Fatty acid metabolism
rno01212 Fatty acid metabolism
ko00310 Lysine degradation
rno00310 Lysine degradation
M00032 Lysine degradation, lysine => saccharopine => acetoacetyl-CoA
M00013 Malonate semialdehyde pathway, propanoyl-CoA => Acetyl-CoA
rno01100 Metabolic pathways
ko00640 Propanoate metabolism
rno00640 Propanoate metabolism
ko00380 Tryptophan metabolism
rno00380 Tryptophan metabolism
ko00280 Valine, leucine and isoleucine degradation
rno00280 Valine, leucine and isoleucine degradation

REACTOME Pathway Links

REACTOME Pathway ID Description
5954472 Acyl chain remodeling of CL
5953503 Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA
5953501 Beta oxidation of hexanoyl-CoA to butanoyl-CoA
5953496 Beta oxidation of lauroyl-CoA to decanoyl-CoA-CoA
5953498 Beta oxidation of myristoyl-CoA to lauroyl-CoA
5953502 Beta oxidation of octanoyl-CoA to hexanoyl-CoA
5953499 Beta oxidation of palmitoyl-CoA to myristoyl-CoA
5953288 Fatty acid, triacylglycerol, and ketone body metabolism
5953473 Glycerophospholipid biosynthesis
5953250 Metabolism
5953289 Metabolism of lipids and lipoproteins
5953287 Mitochondrial Fatty Acid Beta-Oxidation
5953497 mitochondrial fatty acid beta-oxidation of saturated fatty acids
5953474 Phospholipid metabolism

Domain Information

InterPro Annotations

Accession Description
IPR006180 3-hydroxyacyl-CoA dehydrogenase, conserved site
IPR006108 3-hydroxyacyl-CoA dehydrogenase, C-terminal
IPR006176 3-hydroxyacyl-CoA dehydrogenase, NAD binding
IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like
IPR029045 ClpP/crotonase-like domain
IPR001753 Crotonase_core_superfam
IPR013328 Dehydrogenase, multihelical
IPR018376 Enoyl-CoA hydratase/isomerase, conserved site
IPR012803 Fatty acid oxidation complex, alpha subunit, mitochondrial
IPR016040 NAD(P)-binding domain

UniProt Annotations

Entry Information

Gene Name
hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), alpha subunit
Protein Entry
ECHA_RAT
UniProt ID
Q64428
Species
Rat

Comments

Comment Type Description
Catalytic Activity (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl- CoA + H(2)O.
Catalytic Activity A long-chain (S)-3-hydroxyacyl-CoA + NAD(+) = a long-chain 3-oxoacyl-CoA + NADH.
Function Bifunctional subunit.
Pathway Lipid metabolism; fatty acid beta-oxidation.
Sequence Caution Sequence=BAA03939.1; Type=Frameshift; Positions=27, 33, 277, 283; Evidence= ;
Similarity In the central section; belongs to the 3-hydroxyacyl- CoA dehydrogenase family
Similarity In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family
Subcellular Location Mitochondrion matrix.
Subunit Octamer of 4 alpha (HADHA) and 4 beta (HADHB) subunits

Identical and Related Proteins

Unique RefSeq proteins for LMP012922 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
148747393 RefSeq NP_570839 763 trifunctional enzyme subunit alpha, mitochondrial precursor

Identical Sequences to LMP012922 proteins

Reference Database Accession Length Protein Name
GI:148747393 GenBank AAH91697.1 763 Hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit [Rattus norvegicus]
GI:148747393 GenBank EDM03000.1 763 rCG61339, isoform CRA_b [Rattus norvegicus]
GI:148747393 GenBank AHH58290.1 763 Sequence 35 from patent US 8617877
GI:148747393 SwissProt Q64428.2 763 RecName: Full=Trifunctional enzyme subunit alpha, mitochondrial; AltName: Full=TP-alpha; Includes: RecName: Full=Long-chain enoyl-CoA hydratase; Includes: RecName: Full=Long chain 3-hydroxyacyl-CoA dehydrogenase; Flags: Precursor [Rattus norvegicus]

Related Sequences to LMP012922 proteins

Reference Database Accession Length Protein Name
GI:148747393 GenBank AAH91697.1 763 Hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit [Rattus norvegicus]
GI:148747393 GenBank EDM03000.1 763 rCG61339, isoform CRA_b [Rattus norvegicus]
GI:148747393 SwissProt Q64428.2 763 RecName: Full=Trifunctional enzyme subunit alpha, mitochondrial; AltName: Full=TP-alpha; Includes: RecName: Full=Long-chain enoyl-CoA hydratase; Includes: RecName: Full=Long chain 3-hydroxyacyl-CoA dehydrogenase; Flags: Precursor [Rattus norvegicus]