Gene/Proteome Database (LMPD)

LMPD ID
LMP012938
Gene ID
171129
Species
Rattus norvegicus (Rat)
Gene Name
UDP-glucose glycoprotein glucosyltransferase 1
Gene Symbol
Uggt1
Synonyms
UGT1; Uggt; rUGT1; Ugcgl1;
Alternate Names
UDP-glucose:glycoprotein glucosyltransferase 1; RUGT; UDP--Glc:glycoprotein glucosyltransferase; UDP-glucose ceramide glucosyltransferase-like 1; UDP-glucose glycoprotein: glucosyltransferase UGGT;
Chromosome
9
Map Location
9q21
EC Number
2.4.1.-
Summary
enzyme that catalyzes the addition of a glucose residue from UDP-glucose to an N -linked Man(9)GlcNAc(2)oligosaccharide [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Uggt1

Proteins

UDP-glucose:glycoprotein glucosyltransferase 1 precursor
Refseq ID NP_598280
Protein GI 19424302
UniProt ID Q9JLA3
mRNA ID NM_133596
Length 1527
MGLLIALALLCLFSLAEANSKAITTSLTTKWFSAPLLLEASEFLAEDSQEKFWSFVEASQNIGSSDQHDTDRSYYDAILEAAFRFLSPLQQNLLKFCLSLRSYSASIQAFQQIAVDEPPPEGCKSFLSVHGKQTCDLGTLESLLLTAPDRPKPLLFKGDHRYPSSNPESPVVIFYSEIGHEEFSNIHHQLISKSNEGKINYVFRHYISNPRKEPVHLSGYGVELAIKSTEYKAKDDTQVKGTEVNTTVIGENDPIDEVQGFLFGKLRELYPSLEGQLKEFRKHLVESTNEMAPLKVWQLQDLSFQTAARILAAPVELALVVMKDISQNFPTKARAITKTAVSAQLRAEVEENQKYFKGTIGLQPGDSALFINGLHIDLDTQDIFSLFDTLRNEARVMEGLHRLGIEGLSLHNILKLNIQPSETDYAVDIRSPAISWVNNLEVDSRYNSWPSSLQELLRPTFPGVIRQIRKNLHNMVFIVDPVHETTAELVSIAEMFLSNHIPLRIGFIFVVNDSEDVDGMQDAGVAVLRAYNYVGQEVDGYHAFQTLTQIYNKVRTGEKVKVEHVVSVLEKKYPYVEVNSILGIDSAYDQNRKEARGYYEQTGVGPLPVVLFNGMPFEKEQLDPDELETITMHKILETTTFFQRAVYLGELSHDQDVVEYIMNQPNVVPRINSRILTAKREYLDLTASNNFYVDDFARFSALDSRGKTAAIANSMNYLTKKGMSSKEIYDDSFIRPVTFWIVGDFDSPSGRQLLYDAIKHQKTSNNVRISMINNPSREISDSSTPVSRAIWAALQTQTSNSAKNFITKMVKEETAEALAAGVDIGEFSVGGMDVSLFKEVFESSRMDFILSHALYCRDVLKLKKGQRVVISNGRIIGPLEDSELFNQDDFHLLENIILKTSGQKIKSHIQQLRVEEDVASDLVMKVDALLSAQPKGEARIEYQFFEDKHSAIKLKPKEGETYYDVVAVVDPVTREAQRLAPLLLVLAQLINMSLRVFMNCQSKLSDMPLKSFYRYVLEPEISFTADNSFAKGPIAKFLDMPQSPLFTLNLNTPESWMVESVRTPYDLDNIYLEEVDSIVAAEYELEYLLLEGHCYDITTGQPPRGLQFTLGTSANPTTVDTIVMANLGYFQLKANPGAWILRLRKGRSDDIYRIYSHDGTDSPPDANDVVVILNNFKSKIIKVKVQKKADMANEDLLSDGTNENESGFWDSFKWGFSGQKTEEVKQDKDDIINIFSVASGHLYERFLRIMMLSVLKNTKTPVKFWFLKNYLSPTFKEFIPYMAKKYNFQYELVQYKWPRWLHQQTEKQRIIWGYKILFLDVLFPLVVDKFLFVDADQIVRTDLKELRDFNLDGAPYGYTPFCDSRREMDGYRFWKSGYWASHLAGRKYHISALYVVDLKKFRKIAAGDRLRGQYQGLSQDPNSLSNLDQDLPNNMIHQVPIKSLPQEWLWCETWCDDASKKRAKTIDLCNNPMTKEPKLEAAVRIVPEWQDYDQEIKQLQTLFQEEKELGTLHEEETQEGSQKHEEL
sig_peptide: 1..18 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 1862 peptide sequence: MGLLIALALLCLFSLAEA mat_peptide: 19..1527 product: UDP-glucose:glycoprotein glucosyltransferase 1 calculated_mol_wt: 172205 peptide sequence: NSKAITTSLTTKWFSAPLLLEASEFLAEDSQEKFWSFVEASQNIGSSDQHDTDRSYYDAILEAAFRFLSPLQQNLLKFCLSLRSYSASIQAFQQIAVDEPPPEGCKSFLSVHGKQTCDLGTLESLLLTAPDRPKPLLFKGDHRYPSSNPESPVVIFYSEIGHEEFSNIHHQLISKSNEGKINYVFRHYISNPRKEPVHLSGYGVELAIKSTEYKAKDDTQVKGTEVNTTVIGENDPIDEVQGFLFGKLRELYPSLEGQLKEFRKHLVESTNEMAPLKVWQLQDLSFQTAARILAAPVELALVVMKDISQNFPTKARAITKTAVSAQLRAEVEENQKYFKGTIGLQPGDSALFINGLHIDLDTQDIFSLFDTLRNEARVMEGLHRLGIEGLSLHNILKLNIQPSETDYAVDIRSPAISWVNNLEVDSRYNSWPSSLQELLRPTFPGVIRQIRKNLHNMVFIVDPVHETTAELVSIAEMFLSNHIPLRIGFIFVVNDSEDVDGMQDAGVAVLRAYNYVGQEVDGYHAFQTLTQIYNKVRTGEKVKVEHVVSVLEKKYPYVEVNSILGIDSAYDQNRKEARGYYEQTGVGPLPVVLFNGMPFEKEQLDPDELETITMHKILETTTFFQRAVYLGELSHDQDVVEYIMNQPNVVPRINSRILTAKREYLDLTASNNFYVDDFARFSALDSRGKTAAIANSMNYLTKKGMSSKEIYDDSFIRPVTFWIVGDFDSPSGRQLLYDAIKHQKTSNNVRISMINNPSREISDSSTPVSRAIWAALQTQTSNSAKNFITKMVKEETAEALAAGVDIGEFSVGGMDVSLFKEVFESSRMDFILSHALYCRDVLKLKKGQRVVISNGRIIGPLEDSELFNQDDFHLLENIILKTSGQKIKSHIQQLRVEEDVASDLVMKVDALLSAQPKGEARIEYQFFEDKHSAIKLKPKEGETYYDVVAVVDPVTREAQRLAPLLLVLAQLINMSLRVFMNCQSKLSDMPLKSFYRYVLEPEISFTADNSFAKGPIAKFLDMPQSPLFTLNLNTPESWMVESVRTPYDLDNIYLEEVDSIVAAEYELEYLLLEGHCYDITTGQPPRGLQFTLGTSANPTTVDTIVMANLGYFQLKANPGAWILRLRKGRSDDIYRIYSHDGTDSPPDANDVVVILNNFKSKIIKVKVQKKADMANEDLLSDGTNENESGFWDSFKWGFSGQKTEEVKQDKDDIINIFSVASGHLYERFLRIMMLSVLKNTKTPVKFWFLKNYLSPTFKEFIPYMAKKYNFQYELVQYKWPRWLHQQTEKQRIIWGYKILFLDVLFPLVVDKFLFVDADQIVRTDLKELRDFNLDGAPYGYTPFCDSRREMDGYRFWKSGYWASHLAGRKYHISALYVVDLKKFRKIAAGDRLRGQYQGLSQDPNSLSNLDQDLPNNMIHQVPIKSLPQEWLWCETWCDDASKKRAKTIDLCNNPMTKEPKLEAAVRIVPEWQDYDQEIKQLQTLFQEEKELGTLHEEETQEGSQKHEEL

Gene Information

Entrez Gene ID
171129
Gene Name
UDP-glucose glycoprotein glucosyltransferase 1
Gene Symbol
Uggt1
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 TAS:RGD C endoplasmic reticulum
GO:0005788 IDA:UniProtKB C endoplasmic reticulum lumen
GO:0005793 IDA:UniProtKB C endoplasmic reticulum-Golgi intermediate compartment
GO:0070062 IEA:Ensembl C extracellular vesicular exosome
GO:0003980 IDA:UniProtKB F UDP-glucose:glycoprotein glucosyltransferase activity
GO:0051082 IDA:UniProtKB F unfolded protein binding
GO:0051084 TAS:UniProtKB P 'de novo' posttranslational protein folding
GO:0097359 IDA:GOC P UDP-glucosylation
GO:0006457 TAS:RGD P protein folding
GO:0006486 IEA:UniProtKB-UniPathway P protein glycosylation

KEGG Pathway Links

KEGG Pathway ID Description
ko04141 Protein processing in endoplasmic reticulum
rno04141 Protein processing in endoplasmic reticulum

REACTOME Pathway Links

REACTOME Pathway ID Description
5953739 Asparagine N-linked glycosylation
5954327 Calnexin/calreticulin cycle
5954329 ER Quality Control Compartment (ERQC)
5953345 Metabolism of proteins
5954326 N-glycan trimming in the ER and Calnexin/Calreticulin cycle
5953728 Post-translational protein modification

Domain Information

InterPro Annotations

Accession Description
IPR029044 Nucleotide-diphospho-sugar transferases
IPR009448 UDP-glucose:Glycoprotein Glucosyltransferase

UniProt Annotations

Entry Information

Gene Name
UDP-glucose glycoprotein glucosyltransferase 1
Protein Entry
UGGG1_RAT
UniProt ID
Q9JLA3
Species
Rat

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=44 uM for UDP-glucose (in the presence of 0.5 uM denatured acid phosphatase) {ECO:0000269|PubMed:10764828, ECO:0000269|PubMed:1533626}; Vmax=34 pmol/h/mg enzyme toward UDP-glucose (in the presence of 0.5 uM denatured acid phosphatase) {ECO:0000269|PubMed:10764828, ECO:0000269|PubMed:1533626}; pH dependence: Optimum pH is 7.6-8.0. {ECO:0000269|PubMed:10764828, ECO:0000269|PubMed:1533626};
Cofactor Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence= ;
Domain N-terminal non-catalytic domain is assumed to mediate recognition of proteins with partial folding defects.
Function Recognizes glycoproteins with minor folding defects. Reglucosylates single N-glycans near the misfolded part of the protein, thus providing quality control for protein folding in the endoplasmic reticulum. Reglucosylated proteins are recognized by calreticulin for recycling to the endoplasmic reticulum and refolding or degradation. {ECO:0000269|PubMed:10764828, ECO:0000269|PubMed:14730348, ECO:0000269|PubMed:15861139}.
Pathway Protein modification; protein glycosylation.
Sequence Caution Sequence=AAF67072.1; Type=Erroneous initiation; Evidence= ;
Similarity Belongs to the glycosyltransferase 8 family
Subcellular Location Endoplasmic reticulum lumen {ECO:0000255|PROSITE-ProRule:PRU10138, ECO:0000269|PubMed:11535823}. Endoplasmic reticulum-Golgi intermediate compartment {ECO:0000255|PROSITE-ProRule:PRU10138, ECO:0000269|PubMed:11535823}.
Subunit Monomer as well as in a tight complex with SEP15. Interacts with METTL23 (By similarity)

Identical and Related Proteins

Unique RefSeq proteins for LMP012938 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
19424302 RefSeq NP_598280 1527 UDP-glucose:glycoprotein glucosyltransferase 1 precursor

Identical Sequences to LMP012938 proteins

Reference Database Accession Length Protein Name
GI:19424302 EMBL CAC34042.1 1527 unnamed protein product [synthetic construct]
GI:19424302 GenBank AAF67072.1 1527 UDP-glucose glycoprotein:glucosyltransferase precursor [Rattus norvegicus]
GI:19424302 GenBank AAM60102.1 1527 Sequence 2 from patent US 6399321

Related Sequences to LMP012938 proteins

Reference Database Accession Length Protein Name
GI:19424302 EMBL CAC34042.1 1527 unnamed protein product [synthetic construct]
GI:19424302 GenBank AAF67072.1 1527 UDP-glucose glycoprotein:glucosyltransferase precursor [Rattus norvegicus]
GI:19424302 GenBank AAM60102.1 1527 Sequence 2 from patent US 6399321
GI:19424302 SwissProt Q9JLA3.2 1551 RecName: Full=UDP-glucose:glycoprotein glucosyltransferase 1; Short=UGT1; Short=rUGT1; AltName: Full=UDP--Glc:glycoprotein glucosyltransferase; AltName: Full=UDP-glucose ceramide glucosyltransferase-like 1; Flags: Precursor [Rattus norvegicus]