Gene/Proteome Database (LMPD)

LMPD ID
LMP012950
Gene ID
171553
Species
Rattus norvegicus (Rat)
Gene Name
alpha 1,3-galactosyltransferase 2
Gene Symbol
A3galt2
Alternate Names
alpha-1,3-galactosyltransferase 2; iGb3S; iGb3 synthase; isoglobotriaosylceramide synthase;
Chromosome
5
Map Location
5q36
EC Number
2.4.1.87
Summary
UDP-galactose: beta-d-galactosyl-1,4-glucosylceramide alpha-1, 3-galactosyltransferase; involved in the synthesis of the isoglobo-series of glycosphingolipids [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for A3galt2

Proteins

alpha-1,3-galactosyltransferase 2
Refseq ID NP_612533
Protein GI 19924055
UniProt ID A0A4Z3
mRNA ID NM_138524
Length 339
MALEGLRAKKRLLWRLFLSAFGLLGLYHYWFKIFRLFEVFIPMGICPMAIMPLLKDNFTGVLRHWARPEVLTCTSWGAPIIWDETFDPHVAEREARRQNLTIGLTVFAVGRYLEKYLEHFLVSAEQYFMVGQNVVYYVFTDRPEAVPHVALGQGRLLRVKPVRREKRWQDVSMARMLTLHEALGGQLGREADYVFCLDVDQYFSGNFGPEVLADLVAQLHAWHFRWPRWMLPYERDKRSAAALSLSEGDFYYHAAVFGGSVAALLKLTAHCATGQQLDREHGIEARWHDESHLNKFFWLSKPTKLLSPEFCWAEEIGWRPEIHHPRLIWAPKEYALVRT

Gene Information

Entrez Gene ID
171553
Gene Name
alpha 1,3-galactosyltransferase 2
Gene Symbol
A3galt2
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005794 IDA:UniProtKB C Golgi apparatus
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0031982 IDA:UniProtKB C vesicle
GO:0047276 IEA:UniProtKB-EC F N-acetyllactosaminide 3-alpha-galactosyltransferase activity
GO:0001962 IEA:Ensembl F alpha-1,3-galactosyltransferase activity
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0016757 IDA:RGD F transferase activity, transferring glycosyl groups
GO:0006688 IDA:UniProtKB P glycosphingolipid biosynthetic process
GO:0030259 IDA:UniProtKB P lipid glycosylation

Domain Information

InterPro Annotations

Accession Description
IPR005076 Glycosyl transferase, family 6
IPR029044 Nucleotide-diphospho-sugar transferases

UniProt Annotations

Entry Information

Gene Name
alpha 1,3-galactosyltransferase 2
Protein Entry
A3LT2_RAT
UniProt ID
A0A4Z3
Species
Rat

Comments

Comment Type Description
Catalytic Activity UDP-alpha-D-galactose + beta-D-galactosyl- (1->4)-beta-N-acetyl-D-glucosaminyl-R = UDP + alpha-D-galactosyl- (1->3)-beta-D-galactosyl-(1->4)-beta-N-acetylglucosaminyl-R.
Cofactor Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence= ;
Domain The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.
Function Synthesizes the galactose-alpha(1,3)-galactose group on the glycosphingolipid isoglobotrihexosylceramide or isogloboside 3 (iGb3) by catalyzing the transfer of galactose from UDP-Galactose to its acceptor molecule Gal-beta-1,4-Glc-ceramide. Can also catalyze the addition of galactose to iGb3 itself to form polygalactose structures. Synthesis of iGb3 is the initial step in the formation of the isoglobo-series glycolipid pathway and is the precursor to isogloboside 4 (iGb4) and isoForssman glycolipids. Can glycosylate only lipids and not proteins and is solely responsible for initiating the synthesis of isoglobo-series glycosphingolipids. {ECO:0000269|PubMed:10854427, ECO:0000269|PubMed:12626403, ECO:0000269|PubMed:17206613}.
Miscellaneous This gene is not expressed in humans, and even if it were to be expressed, this enzyme appears to be inactive because of multiple mutations including an amino-acid substitution Leu-187 to Pro and Tyr-252 to Asn.
Similarity Belongs to the glycosyltransferase 6 family
Subcellular Location Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein {ECO:0000269|PubMed:17206613, ECO:0000269|PubMed:18630988}. Note=Also found in numerous large vesicles throughout the cytoplasm of the soma.
Tissue Specificity Dorsal root ganglia neurons (at protein level). High levels in spleen, thymus and skeletal muscle, intermediate levels in lung, uterus, pituitary and heart, low levels in brain, and undetected in adrenal gland and liver. {ECO:0000269|PubMed:10854427, ECO:0000269|PubMed:12626403, ECO:0000269|PubMed:17206613}.

Identical and Related Proteins

Unique RefSeq proteins for LMP012950 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
19924055 RefSeq NP_612533 339 alpha-1,3-galactosyltransferase 2

Identical Sequences to LMP012950 proteins

Reference Database Accession Length Protein Name
GI:19924055 GenBank AAF82757.1 339 iGb3 synthase [Rattus norvegicus]
GI:19924055 GenBank AAH61714.1 339 Alpha 1,3-galactosyltransferase 2 [Rattus norvegicus]
GI:19924055 GenBank EDL80508.1 339 alpha 1,3-galactosyltransferase 2 (isoglobotriaosylceramide synthase), isoform CRA_a [Rattus norvegicus]
GI:19924055 SwissProt A0A4Z3.1 339 RecName: Full=Alpha-1,3-galactosyltransferase 2; Short=A3galt2; AltName: Full=Isoglobotriaosylceramide synthase; Short=iGb3 synthase; Short=iGb3S [Rattus norvegicus]

Related Sequences to LMP012950 proteins

Reference Database Accession Length Protein Name
GI:19924055 GenBank AAF82757.1 339 iGb3 synthase [Rattus norvegicus]
GI:19924055 GenBank AAH61714.1 339 Alpha 1,3-galactosyltransferase 2 [Rattus norvegicus]
GI:19924055 GenBank EDL80508.1 339 alpha 1,3-galactosyltransferase 2 (isoglobotriaosylceramide synthase), isoform CRA_a [Rattus norvegicus]
GI:19924055 SwissProt A0A4Z3.1 339 RecName: Full=Alpha-1,3-galactosyltransferase 2; Short=A3galt2; AltName: Full=Isoglobotriaosylceramide synthase; Short=iGb3 synthase; Short=iGb3S [Rattus norvegicus]