Gene/Proteome Database (LMPD)

LMPD ID
LMP012989
Gene ID
266604
Species
Rattus norvegicus (Rat)
Gene Name
arachidonate 15-lipoxygenase, type B
Gene Symbol
Alox15b
Alternate Names
arachidonate 15-lipoxygenase B; 15-LOX-2; 15-LOX-B; 15-lipoxygenase 2; linoleate 13-lipoxygenase 15-LOb; arachidonate 15-lipoxygenase type II; arachidonate 15-lipoxygenase, second type;
Chromosome
10
Map Location
10q24
EC Number
1.13.11.33
Summary
human homolog is an epidermis-type lipoxygenase and member of the multigene family of mammalian lipoxygenases [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Alox15b

Proteins

arachidonate 15-lipoxygenase B
Refseq ID NP_695213
Protein GI 23463277
UniProt ID Q8K4F2
mRNA ID NM_153301
Length 677
MAKFRVRVSTGEACGAGTWDKVSVSIVGTHGESPLVPLDHLGKEFSAGAEEDFEVTLPQDVGTVLMLRIHKAPPEAPLPLLSFPPDAWYCRWFELEWLPGAALRFPCYQWLEGAGELVLREGAAKVSWQDHHRTLQDQRQKELESRKDMYSWKTYIEGWPHCLDHETVKDLDLNIKYSAMKNAKFFFKAQSAFTELKFKGLLDRTGLWRSLREMKRMFNFHNTPAAEYVFAHWQEDAFFASQFLNGLNPVLIRRCRRLPENFPVTDEMVAPVLGPGTSLQAELEKGSLFLVDHGILSGVQTNVINGKPQFSAAPMTLLYQSPGSGPLLPIAIQLKQTPGPDNPIFLPSDDKWDWLLAKTWVRNAEFSIHEALTHLLHAHLIPEVFALATLRQLPHCHPLFKLLIPHTRYTLHINTLARELLIAPGKVVDKSTGLGIGGFSDLIKRNMEQLSYSVLCLPEDIRARDVGDLPGYYYRDDGMQIWSAIRSFVSEIVDIYYPSDASVRDDQELQAWVGEIFSEGFLSQESSGMPSLLDTQEALVQYVTMVIFTCSAKHAAVSASQFDSCVWMPNLPPSMQLPPPTSKGQASPEGFIATLPAVNATCDVIIALWLLSKEPGDRRPLGHYPDEHFTEEVPRRSIAAFQRKLIQISSGIRKRNQSLALPYTYLDPPLIENSVSI

Gene Information

Entrez Gene ID
266604
Gene Name
arachidonate 15-lipoxygenase, type B
Gene Symbol
Alox15b
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005856 IEA:UniProtKB-KW C cytoskeleton
GO:0005829 ISS:UniProtKB C cytosol
GO:0019898 ISS:UniProtKB C extrinsic component of membrane
GO:0005622 ISS:UniProtKB C intracellular
GO:0005634 IEA:UniProtKB-KW C nucleus
GO:0005886 IEA:UniProtKB-KW C plasma membrane
GO:0050473 IDA:UniProtKB F arachidonate 15-lipoxygenase activity
GO:0005509 ISS:UniProtKB F calcium ion binding
GO:0005506 ISS:UniProtKB F iron ion binding
GO:0008289 IEA:UniProtKB-KW F lipid binding
GO:0019369 IDA:UniProtKB P arachidonic acid metabolic process
GO:0051122 IDA:UniProtKB P hepoxilin biosynthetic process
GO:0019372 IDA:UniProtKB P lipoxygenase pathway

KEGG Pathway Links

KEGG Pathway ID Description
ko00590 Arachidonic acid metabolism
rno00590 Arachidonic acid metabolism
rno01100 Metabolic pathways
rno04726 Serotonergic synapse

Domain Information

InterPro Annotations

Accession Description
IPR008976 Lipase/lipooxygenase, PLAT/LH2
IPR000907 Lipoxygenase
IPR013819 Lipoxygenase, C-terminal
IPR020834 Lipoxygenase, conserved site
IPR020833 Lipoxygenase, iron binding site
IPR001885 Lipoxygenase, mammalian
IPR001024 PLAT/LH2 domain

UniProt Annotations

Entry Information

Gene Name
arachidonate 15-lipoxygenase, type B
Protein Entry
LX15B_RAT
UniProt ID
Q8K4F2
Species
Rat

Comments

Comment Type Description
Catalytic Activity Arachidonate + O(2) = (5Z,8Z,11Z,13E)-(15S)- 15-hydroperoxyicosa-5,8,11,13-tetraenoate
Catalytic Activity Linoleate + O(2) = (9Z,11E)-13- hydroxyoctadeca-9,11-dienoate
Cofactor Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence= ; Note=Binds 1 Fe cation per subunit. {ECO:0000255|PROSITE- ProRule:PRU00726};
Domain The PLAT domain can bind calcium ions; this promotes association with membranes
Function Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid to 15S- hydroperoxyeicosatetraenoic acid/(15S)-HPETE. Also acts on linoleic acid to produce 13-hydroxyoctadecadienoic acid/13-HPODE. Has no detectable 8S-lipoxygenase activity but reacts with (8S)- HPETE to produce (8S,15S)-diHPETE. May regulate progression through the cell cycle and cell proliferation. May also regulate cytokine secretion by macrophages and therefore play a role in the immune response. May also regulate macrophage differentiation into proatherogenic foam cells
Pathway Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis.
Similarity Belongs to the lipoxygenase family
Similarity Contains 1 PLAT domain. {ECO:0000255|PROSITE- ProRule:PRU00152}.
Similarity Contains 1 lipoxygenase domain. {ECO:0000255|PROSITE- ProRule:PRU00726}.
Subcellular Location Cytoplasm, cytosol . Cell membrane {ECO:0000250}. Cytoplasm, cytoskeleton . Nucleus {ECO:0000250}. Membrane ; Peripheral membrane protein . Note=Predominantly cytosolic; becomes enriched at membranes upon calcium binding

Identical and Related Proteins

Unique RefSeq proteins for LMP012989 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
23463277 RefSeq NP_695213 677 arachidonate 15-lipoxygenase B

Identical Sequences to LMP012989 proteins

Reference Database Accession Length Protein Name
GI:23463277 GenBank AAN03708.1 677 15-lipoxygenase-2 [Rattus norvegicus]
GI:23463277 SwissProt Q8K4F2.1 677 RecName: Full=Arachidonate 15-lipoxygenase B; Short=15-LOX-B; AltName: Full=15-lipoxygenase 2; Short=15-LOX-2; AltName: Full=Arachidonate 15-lipoxygenase type II; AltName: Full=Linoleate 13-lipoxygenase 15-LOb [Rattus norvegicus]

Related Sequences to LMP012989 proteins

Reference Database Accession Length Protein Name
GI:23463277 GenBank AAN03708.1 677 15-lipoxygenase-2 [Rattus norvegicus]
GI:23463277 GenBank EDM04847.1 677 arachidonate 15-lipoxygenase, second type [Rattus norvegicus]
GI:23463277 RefSeq XP_008766001.1 636 PREDICTED: arachidonate 15-lipoxygenase B isoform X1 [Rattus norvegicus]
GI:23463277 SwissProt Q8K4F2.1 677 RecName: Full=Arachidonate 15-lipoxygenase B; Short=15-LOX-B; AltName: Full=15-lipoxygenase 2; Short=15-LOX-2; AltName: Full=Arachidonate 15-lipoxygenase type II; AltName: Full=Linoleate 13-lipoxygenase 15-LOb [Rattus norvegicus]