Gene/Proteome Database (LMPD)

LMPD ID
LMP013005
Gene ID
286976
Species
Rattus norvegicus (Rat)
Gene Name
glucosaminyl (N-acetyl) transferase 3, mucin type
Gene Symbol
Gcnt3
Synonyms
dI/C2/C4GnT; beta-16-N-acetylglucosaminyltransferase;
Alternate Names
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3; dIGnT; C2GnT-M; C2GnT-mucin type; beta-1,6-N-acetylglucosaminyltransferase;
Chromosome
8
Map Location
8q24
EC Number
2.4.1.102
Summary
glycosylation enzyme; transfers glycosyl groups to mucins; controls expression of type I antigens on cell surface [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Gcnt3

Proteins

beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
Refseq ID NP_775434
Protein GI 27545396
UniProt ID Q8CH87
mRNA ID NM_173312
Length 437
MVSWRRFCWHYHGWTLGCYMLLAIIALKLSLRLKCDFDVMDLDSKEFQSQYCRDLLYKTLELPAKSSINCSGVIRGEQKAVTQALLNNLELKRKRQSFTEADYLSMTADCEHFKTQRKFIQVPLSKEEANFPIAYSMVIHEKIENFERLLRAVYTPQNIYCVHVDQKSSETFQQAVRAIVSCFPNVFIANKLVSVVYASWSRVQADLNCMEDLLQSPVPWEYLLNTCGTDFPIKTNAEMVKALKLLNGQNSMESEVPPPHKTFRWKYHYEVADTLYRTSKEKTPPPNNITMFTGNAYMVASRDFIEHVLSNSKARQLIEWVKDTYSPDEHLWATLQRASWMPGSDPLHPKFDLSDMRSIARLTKWQDHEGDIENGAPYTSCSGIHQRAICVYGSGDLHWILQNHHLLANKFDPKVDDNVLQCLEEYLRHKAIYGTEL

Gene Information

Entrez Gene ID
286976
Gene Name
glucosaminyl (N-acetyl) transferase 3, mucin type
Gene Symbol
Gcnt3
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005794 IEA:UniProtKB-KW C Golgi apparatus
GO:0070062 IEA:Ensembl C extracellular vesicular exosome
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0008109 IDA:RGD F N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity
GO:0047225 IEA:Ensembl F acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity
GO:0003829 IEA:UniProtKB-EC F beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity
GO:0002426 IEA:Ensembl P immunoglobulin production in mucosal tissue
GO:0050892 IEA:Ensembl P intestinal absorption
GO:0060993 IEA:Ensembl P kidney morphogenesis
GO:0006486 IEA:UniProtKB-UniPathway P protein glycosylation
GO:0048729 IEA:Ensembl P tissue morphogenesis

KEGG Pathway Links

KEGG Pathway ID Description
rno01100 Metabolic pathways
ko00512 Mucin type O-Glycan biosynthesis
rno00512 Mucin type O-Glycan biosynthesis
M00056 O-glycan biosynthesis, mucin type core

REACTOME Pathway Links

REACTOME Pathway ID Description
5953345 Metabolism of proteins
5954369 O-linked glycosylation
5954368 O-linked glycosylation of mucins
5953728 Post-translational protein modification

Domain Information

InterPro Annotations

Accession Description
IPR003406 Glycosyl transferase, family 14

UniProt Annotations

Entry Information

Gene Name
glucosaminyl (N-acetyl) transferase 3, mucin type
Protein Entry
GCNT3_RAT
UniProt ID
Q8CH87
Species
Rat

Comments

Comment Type Description
Biophysicochemical Properties pH dependence: Optimum pH is 7-8.5. ;
Catalytic Activity UDP-N-acetyl-D-glucosamine + beta-D- galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D- galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D- galactosaminyl-R.
Catalytic Activity UDP-N-acetyl-D-glucosamine + beta-D- galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D- glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.
Function Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.
Pathway Protein modification; protein glycosylation.
Ptm N-glycosylated
Similarity Belongs to the glycosyltransferase 14 family
Subcellular Location Golgi apparatus membrane ; Single-pass type II membrane protein .

Identical and Related Proteins

Unique RefSeq proteins for LMP013005 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
27545396 RefSeq NP_775434 437 beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3

Identical Sequences to LMP013005 proteins

Reference Database Accession Length Protein Name
GI:27545396 GenBank EDL84193.1 437 glucosaminyl (N-acetyl) transferase 3, mucin type, isoform CRA_a [Rattus norvegicus]
GI:27545396 RefSeq XP_006243428.1 437 PREDICTED: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 isoform X1 [Rattus norvegicus]
GI:27545396 RefSeq XP_008764442.1 437 PREDICTED: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 isoform X1 [Rattus norvegicus]
GI:27545396 SwissProt Q8CH87.1 437 RecName: Full=Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3; AltName: Full=C2GnT-mucin type; Short=C2GnT-M; AltName: Full=dI/C2/C4GnT; Short=dIGnT [Rattus norvegicus]

Related Sequences to LMP013005 proteins

Reference Database Accession Length Protein Name
GI:27545396 DBBJ BAC53607.1 437 beta-1,6-N-acetylglucosaminyltransferase [Rattus norvegicus]
GI:27545396 RefSeq XP_006243428.1 437 PREDICTED: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 isoform X1 [Rattus norvegicus]
GI:27545396 RefSeq XP_008764442.1 437 PREDICTED: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 isoform X1 [Rattus norvegicus]
GI:27545396 SwissProt Q8CH87.1 437 RecName: Full=Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3; AltName: Full=C2GnT-mucin type; Short=C2GnT-M; AltName: Full=dI/C2/C4GnT; Short=dIGnT [Rattus norvegicus]