Gene/Proteome Database (LMPD)

LMPD ID
LMP013010
Gene ID
287424
Species
Rattus norvegicus (Rat)
Gene Name
arachidonate lipoxygenase 3
Gene Symbol
Aloxe3
Synonyms
eLOX-3; e-LOX-3;
Alternate Names
hydroperoxide isomerase ALOXE3; epidermal LOX-3; epidermis-type lipoxygenase 3; hydroperoxy icosatetraenoate dehydratase;
Chromosome
10
Map Location
10q24
EC Number
5.4.4.7

Proteins

hydroperoxide isomerase ALOXE3
Refseq ID NP_001099263
Protein GI 157786622
UniProt ID D3ZKX9
mRNA ID NM_001105793
Length 711
MAVYRLCVTTGSYLKAGTLDNIYATLVGTCGESPKQKLDRVGRDFATGSVQKYKVRCASELGEILLLRLHKERFAFFCKDPWYCSRICVTTPDGSVVHFPCYQWIDGYCTVELRPGTARTICQDALPLLLDHRKRELQARQECYRWKIYAPGFPRMVDVSSFEEMESDKKFALTKTAPCADQDDNSGNRYLPGFPMKVDIPSLLHMEPNIRYSATKTASLIFNALPASLGMKIRGLLDRKGSWKRLDDIRNIFWCHKTFTSEYVTEHWCEDSFFGYQYLNGVNPIMLHCLSSLPSKLPVTNDMVAPLLGPGTCLQTELERGHIFLADYWILAEAPVHCLNGRQQYVTAPLCLLWLNPQGVLLPLAIQLSQIPGPESPIFLPTDCELDWLLAKTWVRNSEFLVHENNTHFLCTHLLCEAFSMATLRQLPLCHPVYKLLLPHTRYTLQVNTIARATLLNPDGLVDKVTSIGRRGLIYLMSTGLAHFTYTDFCLPDSLRARGVLTIPNYHYRDDGLKIWAAIERFVSEIVSYYYPNDACVQQDSELQAWVGEIFAQAFLGRESSGFPSRLCTPGELVKYLTAIIFNCSAQHAAVNSGQHDFGAWMPNAPSSMRQPPPQTKGNTTMESYLETLPEVNTTCSNLLLFWLVSQEPKDQRPLGTYPDEHFTEEAPRQSIAAFQKCLAQISKDIRARNESLALPYAYLDPPLIENSVSI

Gene Information

Entrez Gene ID
287424
Gene Name
arachidonate lipoxygenase 3
Gene Symbol
Aloxe3
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IEA:UniProtKB-KW C cytoplasm
GO:0051120 ISS:UniProtKB F hepoxilin A3 synthase activity
GO:0005506 IEA:InterPro F iron ion binding
GO:0016829 IEA:UniProtKB-KW F lyase activity
GO:0016702 ISS:UniProtKB F oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
GO:0019369 IDA:UniProtKB P arachidonic acid metabolic process
GO:0046513 ISS:UniProtKB P ceramide biosynthetic process
GO:0061436 ISS:UniProtKB P establishment of skin barrier
GO:0045444 ISS:UniProtKB P fat cell differentiation
GO:0051122 IDA:UniProtKB P hepoxilin biosynthetic process
GO:0043651 ISS:UniProtKB P linoleic acid metabolic process
GO:0019372 ISS:UniProtKB P lipoxygenase pathway
GO:0035357 ISS:UniProtKB P peroxisome proliferator activated receptor signaling pathway
GO:0019233 IMP:UniProtKB P sensory perception of pain
GO:0006665 ISS:UniProtKB P sphingolipid metabolic process

Domain Information

InterPro Annotations

Accession Description
IPR008976 Lipase/lipooxygenase, PLAT/LH2
IPR000907 Lipoxygenase
IPR013819 Lipoxygenase, C-terminal
IPR020834 Lipoxygenase, conserved site
IPR020833 Lipoxygenase, iron binding site
IPR001885 Lipoxygenase, mammalian
IPR001024 PLAT/LH2 domain

UniProt Annotations

Entry Information

Gene Name
arachidonate lipoxygenase 3
Protein Entry
LOXE3_RAT
UniProt ID
D3ZKX9
Species
Rat

Comments

Comment Type Description
Catalytic Activity A hydroperoxy icosatetraenoate = a hydroxy epoxy icosatrienoate.
Catalytic Activity A hydroperoxy icosatetraenoate = an oxoicosatetraenoate + H(2)O.
Cofactor Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence= ; Note=Binds 1 Fe cation per subunit. {ECO:0000255|PROSITE- ProRule:PRU00726};
Function Non-heme iron-containing lipoxygenase which is atypical in that it displays a prominent hydroperoxide isomerase activity and a reduced dioxygenase activity compared to other lipoxygenases. The hydroperoxide isomerase activity catalyzes the isomerization of hydroperoxides, derived from arachidonic and linoleic acid by ALOX12B, into hepoxilin-type epoxyalcohols. The dioxygenase activity requires a step of activation of the enzyme by molecular oxygen. In presence of oxygen, oxygenates polyunsaturated fatty acids, including arachidonic acid, to produce fatty acid hydroperoxides. In the skin, acts downstream of ALOX12B on the linoleate moiety of esterified omega-hydroxyacyl- sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. In parallel, it may have a signaling function in barrier formation through the production of hepoxilins metabolites. Plays also a role in adipocyte differentiation through hepoxilin A3 and hepoxilin B3 production which in turn activates PPARG. Through the production of hepoxilins in the spinal cord, it may regulate inflammatory tactile allodynia
Pathway Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis.
Pathway Lipid metabolism; sphingolipid metabolism.
Similarity Belongs to the lipoxygenase family
Similarity Contains 1 PLAT domain. {ECO:0000255|PROSITE- ProRule:PRU00152}.
Similarity Contains 1 lipoxygenase domain. {ECO:0000255|PROSITE- ProRule:PRU00726}.
Subcellular Location Cytoplasm {ECO:0000255|PROSITE- ProRule:PRU00726}.
Web Resource Name=Protein Spotlight; Note=about water - Issue 153 of September 2013; URL="http://web.expasy.org/spotlight/back_issues/153/";

Identical and Related Proteins

Unique RefSeq proteins for LMP013010 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
157786622 RefSeq NP_001099263 711 hydroperoxide isomerase ALOXE3

Identical Sequences to LMP013010 proteins

Reference Database Accession Length Protein Name
GI:157786622 GenBank EDM04845.1 711 arachidonate lipoxygenase 3 (predicted) [Rattus norvegicus]
GI:157786622 RefSeq XP_006246679.1 711 PREDICTED: hydroperoxide isomerase ALOXE3 isoform X1 [Rattus norvegicus]
GI:157786622 SwissProt D3ZKX9.1 711 RecName: Full=Hydroperoxide isomerase ALOXE3; AltName: Full=Epidermis-type lipoxygenase 3; Short=Epidermal LOX-3; Short=e-LOX-3; Short=eLOX-3; AltName: Full=Hydroperoxy icosatetraenoate dehydratase [Rattus norvegicus]

Related Sequences to LMP013010 proteins

Reference Database Accession Length Protein Name
GI:157786622 GenBank EDM04845.1 711 arachidonate lipoxygenase 3 (predicted) [Rattus norvegicus]
GI:157786622 RefSeq XP_006246679.1 711 PREDICTED: hydroperoxide isomerase ALOXE3 isoform X1 [Rattus norvegicus]
GI:157786622 SwissProt Q9WV07.2 711 RecName: Full=Hydroperoxide isomerase ALOXE3; AltName: Full=Epidermis-type lipoxygenase 3; Short=Epidermal LOX-3; Short=e-LOX-3; Short=eLOX-3; AltName: Full=Hydroperoxy icosatetraenoate dehydratase [Mus musculus]
GI:157786622 SwissProt D3ZKX9.1 711 RecName: Full=Hydroperoxide isomerase ALOXE3; AltName: Full=Epidermis-type lipoxygenase 3; Short=Epidermal LOX-3; Short=e-LOX-3; Short=eLOX-3; AltName: Full=Hydroperoxy icosatetraenoate dehydratase [Rattus norvegicus]