Gene/Proteome Database (LMPD)

LMPD ID
LMP013011
Gene ID
287425
Species
Rattus norvegicus (Rat)
Gene Name
arachidonate 12-lipoxygenase, 12R type
Gene Symbol
Alox12b
Alternate Names
arachidonate 12-lipoxygenase, 12R-type; 12R-LOX; 12R-lipoxygenase; epidermis-type lipoxygenase 12; alox12b {ECO:0000312|RGD:1305330};
Chromosome
10
Map Location
10q24
EC Number
1.13.11.-

Proteins

arachidonate 12-lipoxygenase, 12R-type
Refseq ID NP_001034466
Protein GI 86439961
UniProt ID Q2KMM4
mRNA ID NM_001039377
Length 701
MATYKVKVATGTDFFSGTLDSISLTIVGTQGESHKQRLNHFGRDFATGAVDDYTVQCQQDLGELIIIRLHKEPHSFLPKDPWYCNYVQICAPNCRVYHFPAYQWMDGYETLSLREATGKTTADDTLPILLEHRQEEIRAKKDFYHWRVFVPGLPNYVDIPSYHPPPRRCRNPNRPEWNGYIPGFPILINIKATRFLNLNLRFSFVKTASFFYRLGPMALAFKLRGLVDRKRSWKRLKDIKNIFPATKTVVSEYVAEHWTEDSFFGYQYLNGINPGHIRRCMQIPDKFPVTDEMVAPFLGEGTCLQAELEKGNIYLADYRILDGIPTVELNGQKQHHCAPICLLHFGPDGNMMPIAIQLSQTPGPDCPIFLPNDSEWDWLLAKTWVRYAEFYSHEAVAHLLESHLIGEAFCLALLRNLPMCHPLYKLLIPHTRYNVQINSIGRALLLNKGGLSARAMSLGLEGFAQVMVRGLSELTYKSLCIPNDFVERGVQDLPGYYFRDDSLAVWYAMERYVTEIITYYYPNDAAVEGDPELQCWVQEIFKECLLERESSGFPTCLRTVPELIEYVTMVMYTCSARHAAVNTGQLEYTSWMPNFPSSMRNPPMQSKGLTTLQTFMDTLPDVKTTCIVLLVLWTLCREPDDRRPLGHFPDIHFVEEAPRRSMEAFRQNLNQISHNIRQRNKCLNLPYYYLDPVLIENSISI

Gene Information

Entrez Gene ID
287425
Gene Name
arachidonate 12-lipoxygenase, 12R type
Gene Symbol
Alox12b
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IEA:UniProtKB-KW C cytoplasm
GO:0004052 ISS:UniProtKB F arachidonate 12-lipoxygenase activity
GO:0005506 IEA:InterPro F iron ion binding
GO:1990136 ISS:UniProtKB F linoleate 9S-lipoxygenase activity
GO:0019369 IDA:UniProtKB P arachidonic acid metabolic process
GO:0046513 ISS:UniProtKB P ceramide biosynthetic process
GO:0061436 ISS:UniProtKB P establishment of skin barrier
GO:0051122 IDA:UniProtKB P hepoxilin biosynthetic process
GO:0043651 ISS:UniProtKB P linoleic acid metabolic process
GO:0019372 ISS:UniProtKB P lipoxygenase pathway
GO:0055114 ISS:UniProtKB P oxidation-reduction process
GO:0010628 ISS:UniProtKB P positive regulation of gene expression
GO:0043410 ISS:UniProtKB P positive regulation of MAPK cascade
GO:0070257 ISS:UniProtKB P positive regulation of mucus secretion
GO:0006497 ISS:UniProtKB P protein lipidation
GO:0006665 ISS:UniProtKB P sphingolipid metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
ko00590 Arachidonic acid metabolism
rno00590 Arachidonic acid metabolism
rno01100 Metabolic pathways
rno04726 Serotonergic synapse

REACTOME Pathway Links

REACTOME Pathway ID Description
5953493 Arachidonic acid metabolism
5953250 Metabolism
5953289 Metabolism of lipids and lipoproteins
5954556 Synthesis of 12-eicosatetraenoic acid derivatives

Domain Information

InterPro Annotations

Accession Description
IPR008976 Lipase/lipooxygenase, PLAT/LH2
IPR000907 Lipoxygenase
IPR020834 Lipoxygenase, conserved site
IPR013819 Lipoxygenase, C-terminal
IPR020833 Lipoxygenase, iron binding site
IPR001885 Lipoxygenase, mammalian
IPR001024 PLAT/LH2 domain

UniProt Annotations

Entry Information

Gene Name
arachidonate 12-lipoxygenase, 12R type
Protein Entry
LX12B_RAT
UniProt ID
Q2KMM4
Species
Rat

Comments

Comment Type Description
Catalytic Activity Arachidonate + O(2) = (5Z,8Z,10E,14Z)-(12R)- 12-hydroperoxyicosa-5,8,10,14-tetraenoate
Cofactor Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence= ; Note=Binds 1 Fe cation per subunit. {ECO:0000255|PROSITE- ProRule:PRU00726};
Function Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12R)- hydroperoxyeicosatetraenoic acid/(12R)-HPETE and minor stereoisomers. In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. May also play a role in the regulation of the expression of airway mucins
Pathway Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis.
Pathway Lipid metabolism; sphingolipid metabolism.
Similarity Belongs to the lipoxygenase family
Similarity Contains 1 lipoxygenase domain. {ECO:0000255|PROSITE- ProRule:PRU00726}.
Similarity Contains 1 PLAT domain. {ECO:0000255|PROSITE- ProRule:PRU00152}.
Subcellular Location Cytoplasm {ECO:0000255|PROSITE- ProRule:PRU00726}.
Web Resource Name=Protein Spotlight; Note=about water - Issue 153 of September 2013; URL="http://web.expasy.org/spotlight/back_issues/153/";

Identical and Related Proteins

Unique RefSeq proteins for LMP013011 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
86439961 RefSeq NP_001034466 701 arachidonate 12-lipoxygenase, 12R-type

Identical Sequences to LMP013011 proteins

Reference Database Accession Length Protein Name
GI:86439961 GenBank AAX85361.1 701 arachidonate 12-lipoxygenase 12R type [Rattus norvegicus]
GI:86439961 GenBank AAX85363.1 701 arachidonate 12-lipoxygenase 12R type [Rattus norvegicus]
GI:86439961 SwissProt Q2KMM4.1 701 RecName: Full=Arachidonate 12-lipoxygenase, 12R-type; Short=12R-LOX; Short=12R-lipoxygenase; AltName: Full=Epidermis-type lipoxygenase 12 [Rattus norvegicus]

Related Sequences to LMP013011 proteins

Reference Database Accession Length Protein Name
GI:86439961 GenBank AAX85361.1 701 arachidonate 12-lipoxygenase 12R type [Rattus norvegicus]
GI:86439961 GenBank AAX85362.1 701 arachidonate 12-lipoxygenase 12R type [Rattus norvegicus]
GI:86439961 GenBank AAX85363.1 701 arachidonate 12-lipoxygenase 12R type [Rattus norvegicus]
GI:86439961 SwissProt Q2KMM4.1 701 RecName: Full=Arachidonate 12-lipoxygenase, 12R-type; Short=12R-LOX; Short=12R-lipoxygenase; AltName: Full=Epidermis-type lipoxygenase 12 [Rattus norvegicus]