Gene/Proteome Database (LMPD)

LMPD ID
LMP013012
Gene ID
287454
Species
Rattus norvegicus (Rat)
Gene Name
arachidonate 12-lipoxygenase
Gene Symbol
Alox12
Synonyms
12-LO; 12S-LOX;
Alternate Names
arachidonate 12-lipoxygenase, 12S-type; platelet 12-LOX; 12S-lipoxygenase; lipoxin synthase 12-LO; platelet-type lipoxygenase 12;
Chromosome
10
Map Location
10q24

Proteins

arachidonate 12-lipoxygenase, 12S-type
Refseq ID NP_001099268
Protein GI 157786628
mRNA ID NM_001105798
Length 663
MGRYRVRVVTGPWLFSGSVNLVRLWLVGAHREARLELQLRPARGKEEEFDFDVAEDLGPLQFVKLHKEHTVVDDAWFCNLITVQGPGMSAEAVFPCYRWVQGEGILRLPEGTARLAGDNALDVFQKHREKELKERQQTYRWATWKQGLPQTIAADCKDDLPPNMRFHEEKRLDFEWTLKAGVLEMGLKRVYTLLRSWNRLEDFDQIFWGQKSALAEKVHRCWQEDELFGYQFLNGANPMLLRRSTSLPSRLVLPPGTEELQAQLEKELKDGCLFEADFILLDGIPANVIRGEQQYLAAPLVMLRMDPSGKLLPMAIQIQPPNPSSPAPTLFLPSDPPLAWLLAKIWVRNSDFQLQELQFHLLNTHLVAEVIAVATMRCLPGLHPIFKLLVPHIRYTMEINTRSRTQLISDGGIFDQVVSTGGGGHVQLLTRAVAQLTYCSLCPPDDLATRGLLRVPSALYAQDALQLWEVTARYVNGMVHLFYQSDDIVRGDPELQAWCREITEVGLCHAQDRGFPVSFQSRAQLCHFLTMCVFTCTSQHAAINQGQLDWYGWVPNAPCTMRMPPPTSKDDVTMETIMGSLPDVQKSCLQMTITWHLGRLQPDMVPLGHHKEKYFSDPRTKAVLSQFQADLENLEKEITARNQQLDLPYEYLKPSRIENSITI

Gene Information

Entrez Gene ID
287454
Gene Name
arachidonate 12-lipoxygenase
Gene Symbol
Alox12
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 ISS:UniProtKB C cytoplasm
GO:0005829 IDA:RGD C cytosol
GO:0070062 IEA:Ensembl C extracellular vesicular exosome
GO:0016020 ISS:UniProtKB C membrane
GO:0042383 ISS:UniProtKB C sarcolemma
GO:0004052 IMP:RGD F arachidonate 12-lipoxygenase activity
GO:0016787 IEA:UniProtKB-KW F hydrolase activity
GO:0005506 IEA:InterPro F iron ion binding
GO:0016165 IEA:Ensembl F linoleate 13S-lipoxygenase activity
GO:0007568 IEP:RGD P aging
GO:0019369 IDA:UniProtKB P arachidonic acid metabolic process
GO:0006928 IEA:Ensembl P cellular component movement
GO:0071396 IEP:RGD P cellular response to lipid
GO:0061436 ISS:UniProtKB P establishment of skin barrier
GO:0019395 IEA:Ensembl P fatty acid oxidation
GO:0051122 IDA:UniProtKB P hepoxilin biosynthetic process
GO:1901751 ISS:UniProtKB P leukotriene A4 metabolic process
GO:0043651 ISS:UniProtKB P linoleic acid metabolic process
GO:2001303 ISS:UniProtKB P lipoxin A4 biosynthetic process
GO:2001306 ISS:UniProtKB P lipoxin B4 biosynthetic process
GO:0019372 IDA:UniProtKB P lipoxygenase pathway
GO:0043066 IMP:RGD P negative regulation of apoptotic process
GO:0010656 ISS:UniProtKB P negative regulation of muscle cell apoptotic process
GO:0090331 ISS:UniProtKB P negative regulation of platelet aggregation
GO:0045766 ISS:UniProtKB P positive regulation of angiogenesis
GO:0043065 IMP:RGD P positive regulation of apoptotic process
GO:0045785 IEA:Ensembl P positive regulation of cell adhesion
GO:0010942 IMP:RGD P positive regulation of cell death
GO:0030307 ISS:UniProtKB P positive regulation of cell growth
GO:0030335 ISS:UniProtKB P positive regulation of cell migration
GO:0008284 IMP:RGD P positive regulation of cell proliferation
GO:0043280 IMP:RGD P positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0045603 IMP:RGD P positive regulation of endothelial cell differentiation
GO:0010595 IMP:RGD P positive regulation of endothelial cell migration
GO:0010628 IMP:RGD P positive regulation of gene expression
GO:0051901 IMP:RGD P positive regulation of mitochondrial depolarization
GO:0048661 IMP:RGD P positive regulation of smooth muscle cell proliferation
GO:0045909 IMP:RGD P positive regulation of vasodilation

KEGG Pathway Links

KEGG Pathway ID Description
ko00590 Arachidonic acid metabolism
rno00590 Arachidonic acid metabolism
ko04750 Inflammatory mediator regulation of TRP channels
rno04750 Inflammatory mediator regulation of TRP channels
rno01100 Metabolic pathways
rno04726 Serotonergic synapse

REACTOME Pathway Links

REACTOME Pathway ID Description
5953493 Arachidonic acid metabolism
5953250 Metabolism
5953289 Metabolism of lipids and lipoproteins
5954556 Synthesis of 12-eicosatetraenoic acid derivatives
5954553 Synthesis of Hepoxilins (HX) and Trioxilins (TrX)
5954552 Synthesis of Lipoxins (LX)

Domain Information

InterPro Annotations

Accession Description
IPR008976 Lipase/lipooxygenase, PLAT/LH2
IPR000907 Lipoxygenase
IPR013819 Lipoxygenase, C-terminal
IPR020834 Lipoxygenase, conserved site
IPR020833 Lipoxygenase, iron binding site
IPR001885 Lipoxygenase, mammalian
IPR001024 PLAT/LH2 domain

UniProt Annotations

Entry Information

Gene Name
arachidonate 12-lipoxygenase
Species
Rat

Comments

Comment Type Description
Catalytic Activity (7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa- 7,9,11,14-tetraenoate + H(2)O = (5S,14R,15S)-trihydroxy- (6E,8Z,10E,12E)-eicosatetraenoate. {ECO:0000269|PubMed:23382512}.
Catalytic Activity (7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa- 7,9,11,14-tetraenoate + H(2)O = (5S,6R,15S)-trihydroxy- (7E,9E,11Z,13E)-eicosatetraenoate. {ECO:0000269|PubMed:23382512}.
Catalytic Activity Arachidonate + O(2) = (5Z,8Z,10E,14Z)-(12S)- 12-hydroperoxyicosa-5,8,10,14-tetraenoate. {ECO:0000269|PubMed:23382512}.
Cofactor Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000255|PROSITE-ProRule:PRU00726}; Note=Binds 1 Fe cation per subunit. {ECO:0000255|PROSITE- ProRule:PRU00726};
Enzyme Regulation Activated by EGF. {ECO:0000250}.
Function Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)- hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation. {ECO:0000269|PubMed:15105833, ECO:0000269|PubMed:23382512}.
Pathway Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis. {ECO:0000269|PubMed:23382512}.
Similarity Belongs to the lipoxygenase family. {ECO:0000305}.
Similarity Contains 1 PLAT domain. {ECO:0000255|PROSITE- ProRule:PRU00152}.
Similarity Contains 1 lipoxygenase domain. {ECO:0000255|PROSITE- ProRule:PRU00726}.
Subcellular Location Cytoplasm, cytosol. Membrane {ECO:0000250}. Note=Membrane association is stimulated by EGF. {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP013012 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
157786628 RefSeq NP_001099268 663 arachidonate 12-lipoxygenase, 12S-type

Identical Sequences to LMP013012 proteins

Reference Database Accession Length Protein Name
GI:157786628 GenBank EDM04998.1 663 arachidonate 12-lipoxygenase (predicted), isoform CRA_b [Rattus norvegicus]

Related Sequences to LMP013012 proteins

Reference Database Accession Length Protein Name
GI:157786628 DBBJ BAE43031.1 663 unnamed protein product [Mus musculus]
GI:157786628 GenBank EDM04998.1 663 arachidonate 12-lipoxygenase (predicted), isoform CRA_b [Rattus norvegicus]
GI:157786628 GenBank AAI65994.1 663 Arachidonate 12-lipoxygenase [synthetic construct]
GI:157786628 SwissProt F1LQ70.1 663 RecName: Full=Arachidonate 12-lipoxygenase, 12S-type; Short=12S-LOX; Short=12S-lipoxygenase; AltName: Full=Lipoxin synthase 12-LO; AltName: Full=Platelet-type lipoxygenase 12 [Rattus norvegicus]