Gene/Proteome Database (LMPD)

LMPD ID
LMP013208
Gene ID
301517
Species
Rattus norvegicus (Rat)
Gene Name
cytochrome P450, family 27, subfamily a, polypeptide 1
Gene Symbol
Cyp27a1
Synonyms
Cyp27; P450C27;
Alternate Names
sterol 26-hydroxylase, mitochondrial; cytochrome P450 27; sterol 27-hydroxylase; cytochrome P-450c27/25; vitamin D(3) 25-hydroxylase; 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase;
Chromosome
9
Map Location
9q33
EC Number
1.14.13.15
Summary
mitochondrial enzyme with both vitamin D3 25-hydroxylase and cholesterol 26-hydroxylase activities [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Cyp27a1

Proteins

sterol 26-hydroxylase, mitochondrial
Refseq ID NP_849178
Protein GI 139949139
UniProt ID P17178
mRNA ID NM_178847
Length 533
MAVLSRMRLRWALLDTRVMGHGLCPQGARAKAAIPAALRDHESTEGPGTGQDRPRLRSLAELPGPGTLRFLFQLFLRGYVLHLHELQALNKAKYGPMWTTTFGTRTNVNLASAPLLEQVMRQEGKYPIRDSMEQWKEHRDHKGLSYGIFITQGQQWYHLRHSLNQRMLKPAEAALYTDALNEVISDFIARLDQVRTESASGDQVPDVAHLLYHLALEAICYILFEKRVGCLEPSIPEDTATFIRSVGLMFKNSVYVTFLPKWSRPLLPFWKRYMNNWDNIFSFGEKMIHQKVQEIEAQLQAAGPDGVQVSGYLHFLLTKELLSPQETVGTFPELILAGVDTTSNTLTWALYHLSKNPEIQEALHKEVTGVVPFGKVPQNKDFAHMPLLKAVIKETLRLYPVVPTNSRIITEKETEINGFLFPKNTQFVLCHYVVSRDPSVFPEPESFQPHRWLRKREDDNSGIQHPFGSVPFGYGVRSCLGRRIAELEMQLLLSRLIQKYEVVLSPGMGEVKSVSRIVLVPSKKVSLRFLQRQ
transit_peptide: 1..32 experiment: experimental evidence, no additional details recorded note: Mitochondrion; propagated from UniProtKB/Swiss-Prot (P17178.1) calculated_mol_wt: 3566 peptide sequence: MAVLSRMRLRWALLDTRVMGHGLCPQGARAKA mat_peptide: 33..533 product: Sterol 26-hydroxylase, mitochondrial experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (P17178.1) calculated_mol_wt: 57185 peptide sequence: AIPAALRDHESTEGPGTGQDRPRLRSLAELPGPGTLRFLFQLFLRGYVLHLHELQALNKAKYGPMWTTTFGTRTNVNLASAPLLEQVMRQEGKYPIRDSMEQWKEHRDHKGLSYGIFITQGQQWYHLRHSLNQRMLKPAEAALYTDALNEVISDFIARLDQVRTESASGDQVPDVAHLLYHLALEAICYILFEKRVGCLEPSIPEDTATFIRSVGLMFKNSVYVTFLPKWSRPLLPFWKRYMNNWDNIFSFGEKMIHQKVQEIEAQLQAAGPDGVQVSGYLHFLLTKELLSPQETVGTFPELILAGVDTTSNTLTWALYHLSKNPEIQEALHKEVTGVVPFGKVPQNKDFAHMPLLKAVIKETLRLYPVVPTNSRIITEKETEINGFLFPKNTQFVLCHYVVSRDPSVFPEPESFQPHRWLRKREDDNSGIQHPFGSVPFGYGVRSCLGRRIAELEMQLLLSRLIQKYEVVLSPGMGEVKSVSRIVLVPSKKVSLRFLQRQ

Gene Information

Entrez Gene ID
301517
Gene Name
cytochrome P450, family 27, subfamily a, polypeptide 1
Gene Symbol
Cyp27a1
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005740 IDA:RGD C mitochondrial envelope
GO:0005743 IEA:Ensembl C mitochondrial inner membrane
GO:0005739 IDA:RGD C mitochondrion
GO:0047749 IEA:UniProtKB-EC F cholestanetriol 26-monooxygenase activity
GO:0031073 IDA:RGD F cholesterol 26-hydroxylase activity
GO:0020037 IEA:InterPro F heme binding
GO:0005506 IEA:InterPro F iron ion binding
GO:0030343 IDA:RGD F vitamin D3 25-hydroxylase activity
GO:0036378 IDA:GOC P calcitriol biosynthetic process from calciol
GO:0008203 IDA:RGD P cholesterol metabolic process
GO:0006706 TAS:RGD P steroid catabolic process

KEGG Pathway Links

KEGG Pathway ID Description
rno01100 Metabolic pathways
ko03320 PPAR signaling pathway
rno03320 PPAR signaling pathway
ko00120 Primary bile acid biosynthesis
rno00120 Primary bile acid biosynthesis

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-6061 bile acid biosynthesis, neutral pathway

REACTOME Pathway Links

REACTOME Pathway ID Description
5953720 Bile acid and bile salt metabolism
5953258 Biological oxidations
5953485 Cytochrome P450 - arranged by substrate type
5953932 Endogenous sterols
5953250 Metabolism
5953289 Metabolism of lipids and lipoproteins
5953326 Phase 1 - Functionalization of compounds
5953930 Synthesis of bile acids and bile salts
5953933 Synthesis of bile acids and bile salts via 24-hydroxycholesterol
5953929 Synthesis of bile acids and bile salts via 27-hydroxycholesterol
5953931 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol

Domain Information

InterPro Annotations

Accession Description
IPR001128 Cytochrome P450
IPR002401 Cytochrome P450, E-class, group I
IPR017972 Cytochrome P450, conserved site

UniProt Annotations

Entry Information

Gene Name
cytochrome P450, family 27, subfamily a, polypeptide 1
Protein Entry
CP27A_RAT
UniProt ID
P17178
Species
Rat

Comments

Comment Type Description
Catalytic Activity 5-beta-cholestane-3-alpha,7-alpha,12-alpha- triol + 3 NADPH + 3 O(2) = (25R)-3-alpha,7-alpha,12-alpha- trihydroxy-5-beta-cholestan-26-oate + 3 NADP(+) + 4 H(2)O.
Cofactor Name=heme; Xref=ChEBI:CHEBI:30413; Evidence= ;
Function Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta- cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3- 25-hydroxylase activity.
Pathway Hormone biosynthesis; cholecalciferol biosynthesis.
Sequence Caution Sequence=AAA86314.1; Type=Erroneous initiation; Evidence= ;
Similarity Belongs to the cytochrome P450 family
Subcellular Location Mitochondrion membrane.
Tissue Specificity Expressed in liver, hepatoma, kidney, ovary and epithelial cells of blood vessels.

Identical and Related Proteins

Unique RefSeq proteins for LMP013208 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
139949139 RefSeq NP_849178 533 sterol 26-hydroxylase, mitochondrial

Identical Sequences to LMP013208 proteins

Reference Database Accession Length Protein Name
GI:139949139 EMBL CAA68822.1 533 vitamin D(3) 25-hydroxylase precursor [Rattus norvegicus]
GI:139949139 GenBank AAH61848.1 533 Cytochrome P450, family 27, subfamily a, polypeptide 1 [Rattus norvegicus]
GI:139949139 SwissProt P17178.1 533 RecName: Full=Sterol 26-hydroxylase, mitochondrial; AltName: Full=5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase; AltName: Full=Cytochrome P-450C27/25; AltName: Full=Cytochrome P450 27; AltName: Full=Sterol 27-hydroxylase; AltName: Full=Vitamin D(3) 25-hydroxylase; Flags: Precursor [Rattus norvegicus]

Related Sequences to LMP013208 proteins

Reference Database Accession Length Protein Name
GI:139949139 EMBL CAA68822.1 533 vitamin D(3) 25-hydroxylase precursor [Rattus norvegicus]
GI:139949139 GenBank AAH61848.1 533 Cytochrome P450, family 27, subfamily a, polypeptide 1 [Rattus norvegicus]
GI:139949139 GenBank EDL75367.1 550 rCG24013, isoform CRA_a [Rattus norvegicus]
GI:139949139 SwissProt P17178.1 533 RecName: Full=Sterol 26-hydroxylase, mitochondrial; AltName: Full=5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase; AltName: Full=Cytochrome P-450C27/25; AltName: Full=Cytochrome P450 27; AltName: Full=Sterol 27-hydroxylase; AltName: Full=Vitamin D(3) 25-hydroxylase; Flags: Precursor [Rattus norvegicus]