LIPID MAPS

LMPD ID

LMP013363

Gene ID

313648

Species

Rattus norvegicus (Rat)

Gene Name

dolichyl-diphosphooligosaccharide-protein glycosyltransferase

Gene Symbol

Ddost

Alternate Names

dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit; DDOST 48 kDa subunit; oligosaccharyl transferase 48 kDa subunit; dolichyl-di-phosphooligosaccharide-protein glycotransferase;

Chromosome

5

Map Location

5q36

EC Number

2.4.99.18

Summary

human homolog catalyzes the transfer of a high-mannose oligosaccharide (GlcNac2Man9Glc3) onto the asparagine acceptor site within an Asn-X-Ser/Thr consensus motif [RGD, Feb 2006]

Orthologs

View orthologs and multiple alignments for Ddost

dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit precursor
Refseq ID	NP_001012104
Protein GI	58865778
UniProt ID	Q641Y0
mRNA ID	NM_001012104
Length	441
MKMVPRLAVRAWPLCGLLLAALGCVCASGPRTLVLLDNLNVRDTHSLFFRSLKDRGFELTFKTADDPSLSLIKYGEFLYDNLIIFSPSVEDFGGNINVETISAFIDGGGSVLVAASSDIGDPLRELGSECGIEFDEEKTAVIDHHNYDVSDLGQHTLIVADTENLLKAPTIVGKSSLNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFPDKPITQYPHAVGRNTLLIAGLQARNNARVIFSGSLDFFSDAFFNSAVQKAAPGAQRYSQTGNYELAVALSRWVFKEEGVLRVGPVSHHRVGETAPPNAYTVTDLVEYSIVIEQLSNGKWVPFDGDDIQLEFVRIDPFVRTFLKRKGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLYSSTQVSVRPLQHTQYERFIPSAYPYYASAFSMMAGLFLFSVVFLHMKEKEKSD
sig_peptide: 1..28 inference: non-experimental evidence, no additional details recorded note: By similarity; propagated from UniProtKB/Swiss-Prot (Q641Y0.1) calculated_mol_wt: 2944 peptide sequence: MKMVPRLAVRAWPLCGLLLAALGCVCAS mat_peptide: 29..441 product: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (Q641Y0.1) calculated_mol_wt: 45970 peptide sequence: GPRTLVLLDNLNVRDTHSLFFRSLKDRGFELTFKTADDPSLSLIKYGEFLYDNLIIFSPSVEDFGGNINVETISAFIDGGGSVLVAASSDIGDPLRELGSECGIEFDEEKTAVIDHHNYDVSDLGQHTLIVADTENLLKAPTIVGKSSLNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFPDKPITQYPHAVGRNTLLIAGLQARNNARVIFSGSLDFFSDAFFNSAVQKAAPGAQRYSQTGNYELAVALSRWVFKEEGVLRVGPVSHHRVGETAPPNAYTVTDLVEYSIVIEQLSNGKWVPFDGDDIQLEFVRIDPFVRTFLKRKGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLYSSTQVSVRPLQHTQYERFIPSAYPYYASAFSMMAGLFLFSVVFLHMKEKEKSD

Entrez Gene ID

313648

Gene Name

dolichyl-diphosphooligosaccharide-protein glycosyltransferase

Gene Symbol

Ddost

Species

Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0016021	IEA:UniProtKB-KW	C	integral component of membrane
GO:0008250	ISS:UniProtKB	C	oligosaccharyltransferase complex
GO:0005886	TAS:Reactome	C	plasma membrane
GO:0004579	IEA:InterPro	F	dolichyl-diphosphooligosaccharide-protein glycotransferase activity
GO:0006486	ISS:UniProtKB	P	protein glycosylation
GO:0018279	IEA:InterPro	P	protein N-linked glycosylation via asparagine

KEGG Pathway Links

KEGG Pathway ID	Description
rno01100	Metabolic pathways
ko00510	N-Glycan biosynthesis
rno00510	N-Glycan biosynthesis
M00072	N-glycosylation by oligosaccharyltransferase
ko04141	Protein processing in endoplasmic reticulum
rno04141	Protein processing in endoplasmic reticulum

InterPro Annotations

Accession	Description
IPR005013	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1

Entry Information

Gene Name

dolichyl-diphosphooligosaccharide-protein glycosyltransferase

Protein Entry

OST48_RAT

UniProt ID

Q641Y0

Species

Rat

Comments

Comment Type	Description
Catalytic Activity	Dolichyl diphosphooligosaccharide + [protein]- L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.
Function	Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains
Pathway	Protein modification; protein glycosylation.
Similarity	Belongs to the DDOST 48 kDa subunit family
Subcellular Location	Endoplasmic reticulum membrane {ECO:0000250}; Single-pass type I membrane protein .
Subunit	Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes even after release from the ribosome. Also identified as part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, VIMP, STT3A AND VCP. This contains known members of the OST complex and may be a form of this complex (By similarity)

Unique RefSeq proteins for LMP013363 (as displayed in Record Overview)

Protein GI	Database	Accession	Length	Protein Name
58865778	RefSeq	NP_001012104	441	dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit precursor

Identical Sequences to LMP013363 proteins

Reference	Database	Accession	Length	Protein Name
GI:58865778	GenBank	AAH82075.1	441	Dolichyl-diphosphooligosaccharide-protein glycosyltransferase [Rattus norvegicus]
GI:58865778	SwissProt	Q641Y0.1	441	RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit; Short=DDOST 48 kDa subunit; Short=Oligosaccharyl transferase 48 kDa subunit; Flags: Precursor [Rattus norvegicus]

Related Sequences to LMP013363 proteins

Reference	Database	Accession	Length	Protein Name
GI:58865778	GenBank	AAH82075.1	441	Dolichyl-diphosphooligosaccharide-protein glycosyltransferase [Rattus norvegicus]
GI:58865778	GenBank	EDL13259.1	441	dolichyl-di-phosphooligosaccharide-protein glycotransferase, isoform CRA_c [Mus musculus]
GI:58865778	RefSeq	XP_006980951.1	441	PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit [Peromyscus maniculatus bairdii]
GI:58865778	SwissProt	Q641Y0.1	441	RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit; Short=DDOST 48 kDa subunit; Short=Oligosaccharyl transferase 48 kDa subunit; Flags: Precursor [Rattus norvegicus]