Gene/Proteome Database (LMPD)
LMPD ID
LMP013379
Gene ID
Species
Rattus norvegicus (Rat)
Gene Name
phosphatidylinositol-4-phosphate 5-kinase, type I, gamma
Gene Symbol
Alternate Names
phosphatidylinositol 4-phosphate 5-kinase type-1 gamma; PIP5KIgamma; PIP5K1-gamma; ptdInsPKIgamma; ptdIns(4)P-5-kinase gamma; ptdIns(4)P-5-kinase 1 gamma; phosphatidylinositol 4-phosphate 5-kinase type I gamma; phosphatidylinositol-4-phosphate 5-kinase type-1 gamma; phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma;
Chromosome
7
Map Location
7q11
EC Number
2.7.1.68
Proteins
| phosphatidylinositol 4-phosphate 5-kinase type-1 gamma isoform b | |
|---|---|
| Refseq ID | NP_001029142 |
| Protein GI | 111185940 |
| UniProt ID | Q5I6B8 |
| mRNA ID | NM_001033970 |
| Length | 636 |
| MELEVPDEAESAEAGAVTAEAAWSAESGAAAGMTQKKAILAEAPLVTGQPGPGHGKKLGHRGVDASGETTYKKTTSSTLKGAIQLGIGYTVGNLSSKPERDVLMQDFYVVESIFFPSEGSNLTPAHHFQDFRFKTYAPVAFRYFRELFGIRPDDYLYSLCNEPLIELSNPGASGSVFYVTSDDEFIIKTVMHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQSGGKNIRVVVMNNVLPRVVKMHLKFDLKGSTYKRRASKKEKEKSLPTYKDLDFMQDMPEGLLLDSDTFGALVKTLQRDCLVLESFKIMDYSLLLGVHNIDQQERERQAEGAQSKADEKRPVAQKALYSTAMESIQGGAARGEAIETDDTMGGIPAVNGRGERLLLHIGIIDILQSYRFIKKLEHTWKALVHDGDTVSVHRPSFYAERFFKFMSSTVFRKSSSLKSSPSKKGRGALLAVKPLGPTAAFSASQIPSEREDVQYDLRGARSYPTLEDEGRPDLLPCTPPSFEEATTASIATTLSSTSLSIPERSPSDTSEQPRYRRRTQSSGQDGRPQEELHAEDLQKITVQVEPVCGVGVVVPKEQGAGVEVPPSGASAAATVEVDAASQASEPASQASDEEDAPSTDIYF | |
| phosphatidylinositol 4-phosphate 5-kinase type-1 gamma isoform c | |
|---|---|
| Refseq ID | NP_001009967 |
| Protein GI | 111185932 |
| UniProt ID | Q5I6B8 |
| mRNA ID | NM_001009967 |
| Length | 688 |
| MELEVPDEAESAEAGAVTAEAAWSAESGAAAGMTQKKAILAEAPLVTGQPGPGHGKKLGHRGVDASGETTYKKTTSSTLKGAIQLGIGYTVGNLSSKPERDVLMQDFYVVESIFFPSEGSNLTPAHHFQDFRFKTYAPVAFRYFRELFGIRPDDYLYSLCNEPLIELSNPGASGSVFYVTSDDEFIIKTVMHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQSGGKNIRVVVMNNVLPRVVKMHLKFDLKGSTYKRRASKKEKEKSLPTYKDLDFMQDMPEGLLLDSDTFGALVKTLQRDCLVLESFKIMDYSLLLGVHNIDQQERERQAEGAQSKADEKRPVAQKALYSTAMESIQGGAARGEAIETDDTMGGIPAVNGRGERLLLHIGIIDILQSYRFIKKLEHTWKALVHDGDTVSVHRPSFYAERFFKFMSSTVFRKSSSLKSSPSKKGRGALLAVKPLGPTAAFSASQIPSEREDVQYDLRGARSYPTLEDEGRPDLLPCTPPSFEEATTASIATTLSSTSLSIPERSPSDTSEQPRYRRRTQSSGQDGRPQEELHAEDLQKITVQVEPVCGVGVVVPKEQGAGVEVPPSGASAAATVEVDAASQASEPASQASDEEDAPSTDIYFFAHGRYWLFSPRRRRLRAVTPSHTGAPTDERSWVYSPLHYSARPASDGESDT | |
Gene Information
Entrez Gene ID
Gene Name
phosphatidylinositol-4-phosphate 5-kinase, type I, gamma
Gene Symbol
Species
Rattus norvegicus
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0030054 | IEA:UniProtKB-KW | C | cell junction |
| GO:0042995 | IEA:UniProtKB-KW | C | cell projection |
| GO:0005737 | IEA:UniProtKB-KW | C | cytoplasm |
| GO:0005886 | IEA:UniProtKB-KW | C | plasma membrane |
| GO:0016308 | IEA:UniProtKB-EC | F | 1-phosphatidylinositol-4-phosphate 5-kinase activity |
| GO:0005524 | IEA:UniProtKB-KW | F | ATP binding |
| GO:0007155 | IEA:UniProtKB-KW | P | cell adhesion |
| GO:0006935 | IEA:UniProtKB-KW | P | chemotaxis |
| GO:0006887 | IEA:UniProtKB-KW | P | exocytosis |
| GO:0006909 | IEA:UniProtKB-KW | P | phagocytosis |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ko04144 | Endocytosis |
| rno04144 | Endocytosis |
| ko04666 | Fc gamma R-mediated phagocytosis |
| rno04666 | Fc gamma R-mediated phagocytosis |
| ko00562 | Inositol phosphate metabolism |
| rno00562 | Inositol phosphate metabolism |
| M00130 | Inositol phosphate metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 |
| rno01100 | Metabolic pathways |
| ko04070 | Phosphatidylinositol signaling system |
| rno04070 | Phosphatidylinositol signaling system |
| ko04810 | Regulation of actin cytoskeleton |
| rno04810 | Regulation of actin cytoskeleton |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| 5953532 | Axon guidance |
| 5953533 | Developmental Biology |
| 5953250 | Metabolism |
| 5953289 | Metabolism of lipids and lipoproteins |
| 5954478 | PI Metabolism |
| 5953474 | Phospholipid metabolism |
| 5954213 | SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion |
| 5954133 | Semaphorin interactions |
| 5954499 | Synthesis of PIPs at the plasma membrane |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR023610 | Phosphatidylinositol-4-phosphate 5-kinase |
| IPR027483 | Phosphatidylinositol-4-phosphate 5-kinase, C-terminal |
| IPR027484 | Phosphatidylinositol-4-phosphate 5-kinase, N-terminal domain |
| IPR002498 | Phosphatidylinositol-4-phosphate 5-kinase, core |
| IPR016034 | Phosphatidylinositol-4-phosphate 5-kinase, core, subgroup |
UniProt Annotations
Entry Information
Gene Name
phosphatidylinositol-4-phosphate 5-kinase, type I, gamma
Protein Entry
PI51C_RAT
UniProt ID
Species
Rat
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | ATP + 1-phosphatidyl-1D-myo-inositol 4- phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate. |
| Enzyme Regulation | Activated by interaction with ARF6. |
| Function | Catalyzes the phosphorylation of phosphatidylinositol 4- phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as vesicle mediated transport, cell adhesion, cell polarization and cell migration. Together with PIP5K1A is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle attachment by generating the pool of PtdIns(4,5)P2 that induces controlled actin depolymerization to facilitate Fc-gamma-R clustering. Mediates RAC1-dependent reorganization of actin filaments. Required for synaptic vesicle transport. Controls the plasma membrane pool of PtdIns(4,5)P2 implicated in synaptic vesicle endocytosis and exocytosis. Plays a role in endocytosis mediated by clathrin and AP-2 (adaptor protein complex 2). Required for clathrin-coated pits assembly at the synapse. Participates in cell junction assembly. Modulates adherens junctions formation by facilitating CDH1 trafficking. Required for focal adhesion dynamics. Modulates the targeting of talins (TLN1 and TLN2) to the plasma membrane and their efficient assembly into focal adhesions. Regulates the interaction between talins (TLN1 and TLN2) and beta-integrins. Required for uropodium formation and retraction of the cell rear during directed migration. Has a role in growth factor- stimulated directional cell migration and adhesion. Required for talin assembly into nascent adhesions forming at the leading edge toward the direction of the growth factor. Negative regulator of T-cell activation and adhesion. Negatively regulates integrin alpha-L/beta-2 (LFA-1) polarization and adhesion induced by T-cell receptor. Together with PIP5K1A have a role during embryogenesis and together with PIP5K1B may have a role immediately after birth (By similarity) |
| Ptm | Acetylation at Lys-265 and Lys-268 seems to decrease lipid kinase activity. Deacetylation of these sites by SIRT1 positively regulates the exocytosis of TSH-containing granules from pituitary cells (By similarity) |
| Ptm | Phosphorylation on Ser-672 negatively regulates binding to TLN2 and is strongly stimulated in mitosis. Phosphorylation on Tyr-671 is necessary for targeting to focal adhesions. Phosphorylation on Ser-672 and Tyr-671 are mutually exclusive. Phosphorylated by SYK and CSK. Tyrosine phosphorylation is enhanced by PTK2 signaling. Phosphorylated at Tyr-635 upon EGF stimulation. Some studies suggest that phosphorylation on Tyr-671 enhances binding to tailins (TLN1 and TLN2) (By similarity); others that phosphorylation at Tyr-671 does not directly enhance binding to tailins (TLN1 and TLN2) but may act indirectly by inhibiting phosphorylation at Ser-672 |
| Similarity | Contains 1 PIPK domain. {ECO:0000255|PROSITE- ProRule:PRU00781}. |
| Subcellular Location | Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Endomembrane system . Cytoplasm . Cell junction, focal adhesion . Cell junction, adherens junction . Cell projection, ruffle membrane . Cell projection, phagocytic cup . Cell projection, uropodium . |
| Subunit | Interacts with TLN1 (By similarity). Interacts with TLN2; interaction stimulates lipid kinase activity (By similarity). May compete with beta-integrins for the same binding site on TLN1 and TLN2 (By similarity). Interacts with ARF6. Interacts with AP2B1 (By similarity). Interacts with AP2M1; phosphorylation of PIP5K1C by CSK disrupts the interaction; clathrin competes with PIP5K1C (By similarity). Interacts with CDH1 (By similarity). Interacts with CSK (By similarity). Interacts with PLCG1; interaction is abolished upon EGF stimulation (By similarity) |
Identical and Related Proteins
Unique RefSeq proteins for LMP013379 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 111185940 | RefSeq | NP_001029142 | 636 | phosphatidylinositol 4-phosphate 5-kinase type-1 gamma isoform b |
| 111185932 | RefSeq | NP_001009967 | 688 | phosphatidylinositol 4-phosphate 5-kinase type-1 gamma isoform c |
Identical Sequences to LMP013379 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:111185940 | GenBank | AAI01910.1 | 636 | Phosphatidylinositol-4-phosphate 5-kinase, type I, gamma [Rattus norvegicus] |
| GI:111185940 | GenBank | EDL89167.1 | 636 | phosphatidylinositol-4-phosphate 5-kinase, type I, gamma [Rattus norvegicus] |
Related Sequences to LMP013379 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:111185932 | GenBank | AAW34235.1 | 688 | phosphatidylinositol 4-phosphate 5-kinase type 1 gamma [Rattus norvegicus] |
| GI:111185940 | GenBank | AAI01910.1 | 636 | Phosphatidylinositol-4-phosphate 5-kinase, type I, gamma [Rattus norvegicus] |
| GI:111185940 | GenBank | EDL89167.1 | 636 | phosphatidylinositol-4-phosphate 5-kinase, type I, gamma [Rattus norvegicus] |
| GI:111185940 | RefSeq | NP_001009967.2 | 688 | phosphatidylinositol 4-phosphate 5-kinase type-1 gamma isoform c [Rattus norvegicus] |
| GI:111185940 | RefSeq | XP_006240997.1 | 662 | PREDICTED: phosphatidylinositol 4-phosphate 5-kinase type-1 gamma isoform X4 [Rattus norvegicus] |
| GI:111185932 | RefSeq | XP_008763323.1 | 692 | PREDICTED: phosphatidylinositol 4-phosphate 5-kinase type-1 gamma isoform X1 [Rattus norvegicus] |
| GI:111185932 | RefSeq | XP_008763324.1 | 690 | PREDICTED: phosphatidylinositol 4-phosphate 5-kinase type-1 gamma isoform X2 [Rattus norvegicus] |
| GI:111185932 | SwissProt | Q5I6B8.1 | 688 | RecName: Full=Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma; Short=PIP5K1-gamma; Short=PtdIns(4)P-5-kinase 1 gamma; AltName: Full=Phosphatidylinositol 4-phosphate 5-kinase type I gamma; Short=PIP5KIgamma [Rattus norvegicus] |