Gene/Proteome Database (LMPD)

LMPD ID
LMP013455
Gene ID
361730
Species
Rattus norvegicus (Rat)
Gene Name
dihydroxyacetone kinase 2 homolog (S. cerevisiae)
Gene Symbol
Dak
Synonyms
RGD1311026;
Alternate Names
bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing);
Chromosome
1
Map Location
1q43
EC Number
2.7.1.28

Proteins

bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)
Refseq ID NP_001034120
Protein GI 84781664
UniProt ID Q4KLZ6
mRNA ID NM_001039031
Length 578
MSSKKMVNSVEGCAGDALAGFVACNPDLQLLQGYRVALRSDLDSLKGRVALLSGGGSGHEPAHAGFIGKGMLTGVIAGAVFASPAVGSILAAIRAVAQAGTAGTLLIVKNYTGDRLNFGLAMEQAKAEGISVEMVVIEDDSAFTVLKKAGRRGLCGTILIHKVAGALAEEGMGLEEITKKVSVIAKAIGTLGVSLSPCSVPGTKPTFELAADEMELGLGIHGEAGVRRIKLVPVDQIVTLMLDHMTDTSNISHVPVKSGSSVVLMVNNLGGLSFLELGIIADAAIRLLEGRGVKVARALVGTFMSALEMRGVSLTLMLVDEPLLKLIDAETNAKAWPHMSKVSVTGRNRIRAAPTEPAEAPEATAAGGVASKQMTLVLDRISTTLIGLEEHLNALDRAAGDGDCGSTHSRAAKAIQGWLKEGPTPASPAQVLSKLSVLLLEKMGGSSGALYGLFLTAAAQPLKANTDLPAWSAAMDAGLKAMQKYGKAAPGDRTMLDSLWAAAQELQAWKSPGASLLPVLTKAVKSAEAAAEATKNMEAGAGRASYISSAQLDQPDPGAVAAAAIFRAILEVLQTKAA

Gene Information

Entrez Gene ID
361730
Gene Name
dihydroxyacetone kinase 2 homolog (S. cerevisiae)
Gene Symbol
Dak
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0070062 IEA:Ensembl C extracellular vesicular exosome
GO:0005634 IEA:Ensembl C nucleus
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0034012 IEA:UniProtKB-EC F FAD-AMP lyase (cyclizing) activity
GO:0004371 IEA:UniProtKB-EC F glycerone kinase activity
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0050354 IEA:UniProtKB-EC F triokinase activity
GO:0046835 IEA:Ensembl P carbohydrate phosphorylation
GO:0006071 IEA:InterPro P glycerol metabolic process
GO:0045088 IEA:Ensembl P regulation of innate immune response

KEGG Pathway Links

KEGG Pathway ID Description
ko01200 Carbon metabolism
rno01200 Carbon metabolism
ko00561 Glycerolipid metabolism
rno00561 Glycerolipid metabolism
rno01100 Metabolic pathways
ko04622 RIG-I-like receptor signaling pathway
rno04622 RIG-I-like receptor signaling pathway

Domain Information

InterPro Annotations

Accession Description
IPR004006 Dak kinase
IPR004007 DhaL domain
IPR012734 Dihydroxyacetone kinase

UniProt Annotations

Entry Information

Gene Name
dihydroxyacetone kinase 2 homolog (S. cerevisiae)
Protein Entry
DHAK_RAT
UniProt ID
Q4KLZ6
Species
Rat

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=8.8 uM for FAD with manganese ; KM=12.5 uM for ADP-glucose with manganese ; KM=652 uM for UDP-glucose with manganese ; KM=606 uM for UDP-galactose with manganese ; KM=714 uM for UDP-xylose with manganese ; KM=107 uM for UDP-glucuronate with manganese ; KM=108 uM for UDP-galacturonate with manganese ; KM=416 uM for CDP-glucose with manganese ; KM=795 uM for CDP-glycerol with manganese ; KM=784 uM for GDP-glucose with manganese ; KM=343 uM for GDP-alpha-L-fucose with manganese ; KM=114 uM for FAD with cobalt ; KM=120 uM for ADP-glucose with cobalt ; KM=2550 uM for UDP-glucose with cobalt ; KM=3661 uM for UDP-galactose with cobalt ; KM=2354 uM for UDP-xylose with cobalt ; KM=539 uM for UDP-glucuronate with cobalt ; KM=759 uM for UDP-galacturonate with cobalt ; KM=3703 uM for CDP-glucose with cobalt ; KM=2031 uM for CDP-glycerol with cobalt ; KM=2246 uM for GDP-glucose with cobalt ; KM=991 uM for GDP-alpha-L-fucose with cobalt ;
Catalytic Activity ATP + D-glyceraldehyde = ADP + D- glyceraldehyde 3-phosphate.
Catalytic Activity ATP + glycerone = ADP + glycerone phosphate.
Catalytic Activity FAD = AMP + riboflavin cyclic-4',5'-phosphate.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence= ;
Cofactor Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence= ; Name=Co(2+); Xref=ChEBI:CHEBI:48828; Evidence= ; Note=Manganese or cobalt are requested for FAD-AMP lyase activity. ;
Enzyme Regulation Each activity is inhibited by the substrate(s) of the other.
Function Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate
Similarity Belongs to the dihydroxyacetone kinase (DAK) family
Similarity Contains 1 DhaK domain. {ECO:0000255|PROSITE- ProRule:PRU00814}.
Similarity Contains 1 DhaL domain. {ECO:0000255|PROSITE- ProRule:PRU00813}.
Subunit Homodimer

Identical and Related Proteins

Unique RefSeq proteins for LMP013455 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
84781664 RefSeq NP_001034120 578 bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)

Identical Sequences to LMP013455 proteins

Reference Database Accession Length Protein Name
GI:84781664 GenBank AAH98925.1 578 Dihydroxyacetone kinase 2 homolog (S. cerevisiae) [Rattus norvegicus]
GI:84781664 GenBank EDM12826.1 578 rCG48042, isoform CRA_a [Rattus norvegicus]
GI:84781664 RefSeq XP_006231118.1 578 PREDICTED: bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing) isoform X1 [Rattus norvegicus]
GI:84781664 SwissProt Q4KLZ6.1 578 RecName: Full=Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing); Includes: RecName: Full=ATP-dependent dihydroxyacetone kinase; Short=DHA kinase; AltName: Full=Glycerone kinase; AltName: Full=Triokinase; AltName: Full=Triose kinase; Includes: RecName: Full=FAD-AMP lyase (cyclizing); AltName: Full=FAD-AMP lyase (cyclic FMN forming); AltName: Full=FMN cyclase [Rattus norvegicus]

Related Sequences to LMP013455 proteins

Reference Database Accession Length Protein Name
GI:84781664 GenBank AAH98925.1 578 Dihydroxyacetone kinase 2 homolog (S. cerevisiae) [Rattus norvegicus]
GI:84781664 GenBank EDM12826.1 578 rCG48042, isoform CRA_a [Rattus norvegicus]
GI:84781664 RefSeq XP_006231118.1 578 PREDICTED: bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing) isoform X1 [Rattus norvegicus]
GI:84781664 SwissProt Q4KLZ6.1 578 RecName: Full=Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing); Includes: RecName: Full=ATP-dependent dihydroxyacetone kinase; Short=DHA kinase; AltName: Full=Glycerone kinase; AltName: Full=Triokinase; AltName: Full=Triose kinase; Includes: RecName: Full=FAD-AMP lyase (cyclizing); AltName: Full=FAD-AMP lyase (cyclic FMN forming); AltName: Full=FMN cyclase [Rattus norvegicus]