Gene/Proteome Database (LMPD)

LMPD ID
LMP013496
Gene ID
363026
Species
Rattus norvegicus (Rat)
Gene Name
coactivator-associated arginine methyltransferase 1
Gene Symbol
Carm1
Synonyms
Prmt4;
Alternate Names
histone-arginine methyltransferase CARM1; protein arginine N-methyltransferase 4; coactivator-associated arginine methyltransferase 1 variant 5;
Chromosome
8
Map Location
8q13
EC Number
2.1.1.-

Proteins

histone-arginine methyltransferase CARM1 isoform a
Refseq ID NP_001025212
Protein GI 71795662
UniProt ID Q4AE70
mRNA ID NM_001030041
Length 608
MAAAAATAVGPGAGSAGVAGPGGAGPCATVSVFPGARLLTIGDANGEIQRHAEQQALRLEVRAGPDAAGIALYSHEDVCVFKCSVSRETECSRVGRQSFIITLGCNSVLIQFATPHDFCSFYNILKTCRGHTLERSVFSERTEESSAVQYFQFYGYLSQQQNMMQDYVRTGTYQRAILQNHTDFKDKIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHAEVLVKSNNLTDRIVVIPGKVEEVSLPEQVDIIISEPMGYMLFNERMLESYLHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHGVDLSALRGAAVDEYFRQPVVDTFDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTVWLSTAPTEPLTHWYQVRCLFQSPLFAKAGDTLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSPSENMWNTGSTYNLSSGVAVAGMPTAYDLSSVIAGGSSVGHNNLIPLANTGIVNHTHSRMGSIMSTGIVQGSSGAQGGGGSSSAHYAVNNQFTMGGPAISMASPMSIPTNTMHYGS
histone-arginine methyltransferase CARM1 isoform d
Refseq ID NP_001029260
Protein GI 77539446
UniProt ID Q4AE70
mRNA ID NM_001034088
Length 585
MAAAAATAVGPGAGSAGVAGPGGAGPCATVSVFPGARLLTIGDANGEIQRHAEQQALRLEVRAGPDAAGIALYSHEDVCVFKCSVSRETECSRVGRQSFIITLGCNSVLIQFATPHDFCSFYNILKTCRGHTLERSVFSERTEESSAVQYFQFYGYLSQQQNMMQDYVRTGTYQRAILQNHTDFKDKIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHAEVLVKSNNLTDRIVVIPGKVEEVSLPEQVDIIISEPMGYMLFNERMLESYLHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHGVDLSALRGAAVDEYFRQPVVDTFDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTVWLSTAPTEPLTHWYQVRCLFQSPLFAKAGDTLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSPSENMWNTGSTYNLSSGVAVAGMPTAYDLSSVIAGGSSVGHNNLIPLGSSGAQGGGGSSSAHYAVNNQFTMGGPAISMASPMSIPTNTMHYGS

Gene Information

Entrez Gene ID
363026
Gene Name
coactivator-associated arginine methyltransferase 1
Gene Symbol
Carm1
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IDA:RGD C cytoplasm
GO:0005829 ISS:UniProtKB C cytosol
GO:0005634 IDA:RGD C nucleus
GO:0042054 ISS:UniProtKB F histone methyltransferase activity
GO:0035642 ISS:UniProtKB F histone methyltransferase activity (H3-R17 specific)
GO:0008469 IBA:RefGenome F histone-arginine N-methyltransferase activity
GO:0030374 ISS:UniProtKB F ligand-dependent nuclear receptor transcription coactivator activity
GO:0070577 ISS:UniProtKB F lysine-acetylated histone binding
GO:0016274 ISS:UniProtKB F protein-arginine N-methyltransferase activity
GO:0035242 ISS:UniProtKB F protein-arginine omega-N asymmetric methyltransferase activity
GO:0003713 ISS:UniProtKB F transcription coactivator activity
GO:0044212 ISS:UniProtKB F transcription regulatory region DNA binding
GO:0008380 IEA:UniProtKB-KW P RNA splicing
GO:0060350 IEA:Ensembl P endochondral bone morphogenesis
GO:0034971 ISS:UniProtKB P histone H3-R17 methylation
GO:0034970 IEA:Ensembl P histone H3-R2 methylation
GO:0034969 ISS:UniProtKB P histone arginine methylation
GO:0030520 IEA:Ensembl P intracellular estrogen receptor signaling pathway
GO:0006397 IEA:UniProtKB-KW P mRNA processing
GO:0032091 IEA:Ensembl P negative regulation of protein binding
GO:0009405 IEA:InterPro P pathogenesis
GO:0019919 IBA:RefGenome P peptidyl-arginine methylation, to asymmetrical-dimethyl arginine
GO:0008284 IMP:RGD P positive regulation of cell proliferation
GO:0045600 ISS:UniProtKB P positive regulation of fat cell differentiation
GO:0003420 IEA:Ensembl P regulation of growth plate cartilage chondrocyte proliferation
GO:0033146 ISS:UniProtKB P regulation of intracellular estrogen receptor signaling pathway
GO:0006355 IBA:RefGenome P regulation of transcription, DNA-templated
GO:0051591 IDA:RGD P response to cAMP
GO:0006351 IEA:UniProtKB-KW P transcription, DNA-templated

REACTOME Pathway Links

REACTOME Pathway ID Description
5954496 Activation of gene expression by SREBF (SREBP)
5954332 BMAL1:CLOCK,NPAS2 activates circadian gene expression
5954609 Chromatin modifying enzymes
5954610 Chromatin organization
5954223 Circadian Clock
5953533 Developmental Biology
5953288 Fatty acid, triacylglycerol, and ketone body metabolism
5953330 Gene Expression
5953833 Generic Transcription Pathway
5953250 Metabolism
5953289 Metabolism of lipids and lipoproteins
5953600 Mitochondrial biogenesis
5953601 Organelle biogenesis and maintenance
5954333 PPARA activates gene expression
5954224 REV-ERBA represses gene expression
5954611 RMTs methylate histone arginines
5954225 RORA activates circadian gene expression
5954497 Regulation of cholesterol biosynthesis by SREBP (SREBF)
5954222 Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)
5953599 Transcriptional activation of mitochondrial biogenesis
5954166 Transcriptional regulation of white adipocyte differentiation
5954528 YAP1- and WWTR1 (TAZ)-stimulated gene expression

Domain Information

InterPro Annotations

Accession Description
IPR020989 Histone-arginine methyltransferase CARM1, N-terminal
IPR025799 Protein arginine N-methyltransferase
IPR029063 S-adenosyl-L-methionine-dependent methyltransferase

UniProt Annotations

Entry Information

Gene Name
coactivator-associated arginine methyltransferase 1
Protein Entry
CARM1_RAT
UniProt ID
Q4AE70
Species
Rat

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=4; Comment=Named isoforms=2.; Name=1; Synonyms=CARM1-v2; IsoId=Q4AE70-1; Sequence=Displayed; Name=2; Synonyms=CARM1-v1; IsoId=Q4AE70-2; Sequence=VSP_020384; Name=3; Synonyms=CARM1-v3; IsoId=Q4AE70-3; Sequence=VSP_020382, VSP_020385; Name=4; Synonyms=CARM1-v4; IsoId=Q4AE70-4; Sequence=VSP_020383;
Catalytic Activity S-adenosyl-L-methionine + arginine-[histone] = S-adenosyl-L-homocysteine + N(omega)-methyl-arginine-[histone].
Developmental Stage Isoforms 2 and 3 are expressed in fetal brain
Enzyme Regulation Methylation of H3R17 (H3R17me) by CARM1 is stimulated by preacetylation of H3 'Lys-18' (H3K18ac) H3 'Lys-23' (H3K23ac) by EP300 and blocked by citrullination of H3 'Arg-17' (H3R17ci) by PADI4
Function Isoform 3 specifically affects pre-mRNA splicing. This activity is independent from methyltransferase activity
Function Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activates transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB- dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs
Ptm Auto-methylated on Arg-551. Methylation enhances transcription coactivator activity. Methylation is required for its role in the regulation of pre-mRNA alternative splicing (By similarity)
Ptm Phosphorylation at Ser-217 interferes with S-adenosyl-L- methionine binding and strongly reduces methyltransferase activity. Phosphorylation at Ser-217 is strongly increased during mitosis, and decreases rapidly to a very low, basal level after entry into the G1 phase of the cell cycle. Phosphorylation at Ser- 217 may promote location in the cytosol (By similarity)
Similarity Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N- methyltransferase family
Similarity Contains 1 SAM-dependent MTase PRMT-type domain
Subcellular Location Nucleus . Cytoplasm . Note=Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane (By similarity)
Subunit Homodimer (Probable). Interacts with NR1H4. Interacts with the C-terminus of NCOA2/GRIP1, NCO3/ACTR and NCOA1/SRC1. Part of a complex consisting of CARM1, EP300/P300 and NCOA2/GRIP1. Interacts with FLII, TP53, myogenic factor MEF2, EP300/P300, TRIM24, CREBBP and CTNNB1. Identified in a complex containing CARM1, TRIM24 and NCOA2/GRIP1. Interacts with NCOA3/SRC3. Interacts with RELA (By similarity). Interacts with SNRPC. {ECO:0000250, ECO:0000269|PubMed:15944154, ECO:0000269|PubMed:17882262, ECO:0000305}.
Tissue Specificity Isoform 1 is expressed at low levels in brain, liver and testis. Isoform 2 is highly expressed in brain, liver, skeletal muscle and testis. Isoform 3 is highly expressed in spleen, liver and kidney. Isoform 4 is expressed in spleen, liver and kidney

Identical and Related Proteins

Unique RefSeq proteins for LMP013496 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
71795662 RefSeq NP_001025212 608 histone-arginine methyltransferase CARM1 isoform a
77539446 RefSeq NP_001029260 585 histone-arginine methyltransferase CARM1 isoform d

Identical Sequences to LMP013496 proteins

Reference Database Accession Length Protein Name
GI:77539446 DBBJ BAE16336.1 585 coactivator-associated arginine methyltransferase 1 variant 4 [Rattus norvegicus]
GI:71795662 DBBJ BAE16333.1 608 coactivator-associated arginine methyltransferase 1 variant 1 [Rattus norvegicus]
GI:77539446 GenBank AAH36974.1 585 Carm1 protein [Mus musculus]
GI:71795662 GenBank EDL78286.1 608 protein arginine N-methyltransferase 4 [Rattus norvegicus]
GI:71795662 GenBank ACQ18155.1 608 Sequence 69 from patent US 7510850
GI:77539446 RefSeq NP_694781.1 585 histone-arginine methyltransferase CARM1 isoform 2 [Mus musculus]
GI:71795662 SwissProt Q9WVG6.2 608 RecName: Full=Histone-arginine methyltransferase CARM1; AltName: Full=Coactivator-associated arginine methyltransferase 1; AltName: Full=Protein arginine N-methyltransferase 4 [Mus musculus]

Related Sequences to LMP013496 proteins

Reference Database Accession Length Protein Name
GI:77539446 DBBJ BAE16336.1 585 coactivator-associated arginine methyltransferase 1 variant 4 [Rattus norvegicus]
GI:77539446 DBBJ BAE16333.1 608 coactivator-associated arginine methyltransferase 1 variant 1 [Rattus norvegicus]
GI:71795662 DBBJ BAE16333.1 608 coactivator-associated arginine methyltransferase 1 variant 1 [Rattus norvegicus]
GI:77539446 GenBank AAH36974.1 585 Carm1 protein [Mus musculus]
GI:71795662 GenBank AAD41265.2 608 protein arginine methyltransferase [Mus musculus]
GI:71795662 RefSeq NP_067506.2 608 histone-arginine methyltransferase CARM1 isoform 1 [Mus musculus]
GI:77539446 RefSeq NP_694781.1 585 histone-arginine methyltransferase CARM1 isoform 2 [Mus musculus]
GI:71795662 SwissProt Q9WVG6.2 608 RecName: Full=Histone-arginine methyltransferase CARM1; AltName: Full=Coactivator-associated arginine methyltransferase 1; AltName: Full=Protein arginine N-methyltransferase 4 [Mus musculus]