LIPID MAPS

(+/-)14,15-EpETrE-d11

LMFA03080028

SCIEX QTRAP 6500 LC-MS/MS System

[M-H]-

Ed Dennis, Oswald Quehenberger, Aaron Armando

Run GNPS MASST Search

Structure

Common name

(+/-)14,15-EpETrE-d11

LMID

LMFA03080028

Systematic name

14,15-epoxy-5Z,8Z,11Z-eicosatrienoic-16,16,17,17,18,18,19,19,20,20,20-d11 acid

m/z

330

Adduct / Ion

[M-H]-

Charge

-1

Ion source

ESI

Instrument

SCIEX QTRAP 6500 LC-MS/MS System

Resolution

N/A

Ion mode

Negative

Collision Energy (CE)

-19

Column

Not Available

Mobile phase A

60/40 Water/Acetonitrile, 0.02% Acetic Acid

Mobile phase B

50/50 Isopropanol/Acetonitrile

Retention time (min)

4.23

Amount injected

10ng

DOI for method

Date acquired

Submitters

Ed Dennis, Oswald Quehenberger, Aaron Armando

Source of standard

Cayman Chemical

(+/-)14,15-EpETrE-d11

LMFA03080028

Sciex 5500, QqQ

[M-H]-

N.Kampschulte, M.A.Chromik, R.Kirchhoff, A.Löwen, K.Mosel, K.Plitzko, L.Scholz, L.Wende, M.Wiebel, N.H.Schebb

Run GNPS MASST Search

Structure

Common name

(+/-)14,15-EpETrE-d11

LMID

LMFA03080028

Systematic name

14,15-epoxy-5Z,8Z,11Z-eicosatrienoic-16,16,17,17,18,18,19,19,20,20,20-d11 acid

m/z

330.3

Adduct / Ion

[M-H]-

Charge

-1

Ion source

ESI

Instrument

Sciex 5500, QqQ

Resolution

N/A

Ion mode

Negative

Collision Energy (CE)

-15 - -25

Column

Zorbax Eclipse Plus C18 reversed phase column (2.1 x 150 mm, particle size 1.8 mm; Agilent, Waldbronn, Germany)

Mobile phase A

95/5 (v/v) 0.1% acetic acid (aq)/Eluent B

Mobile phase B

800/150/1 (v/v/v) acetonitrile/methanol/acetic acid

Retention time (min)

22.89

Amount injected

~ 0.3 ng

DOI for method

10.1016/j.aca.2017.11.002

Date acquired

April 2025

Submitters

N.Kampschulte, M.A.Chromik, R.Kirchhoff, A.Löwen, K.Mosel, K.Plitzko, L.Scholz, L.Wende, M.Wiebel, N.H.Schebb

Source of standard

Cayman Chemical

(+/-)14,15-EpETrE-d11

LMFA03080028

Sciex 6500+/QTrap

[M-H]-

N.Kampschulte, M.A.Chromik, R.Kirchhoff, A.Löwen, K.Mosel, K.Plitzko, L.Scholz, L.Wende, M.Wiebel, N.H.Schebb

Run GNPS MASST Search

Structure

Common name

(+/-)14,15-EpETrE-d11

LMID

LMFA03080028

Systematic name

14,15-epoxy-5Z,8Z,11Z-eicosatrienoic-16,16,17,17,18,18,19,19,20,20,20-d11 acid

m/z

330.3

Adduct / Ion

[M-H]-

Charge

-1

Ion source

ESI

Instrument

Sciex 6500+/QTrap

Resolution

N/A

Ion mode

Negative

Collision Energy (CE)

-15 - -25

Column

Zorbax Eclipse Plus C18 reversed phase column (2.1 x 150 mm, particle size 1.8 mm; Agilent, Waldbronn, Germany)

Mobile phase A

95/5 (v/v) 0.1% acetic acid (aq)/Eluent B

Mobile phase B

800/150/1 (v/v/v) acetonitrile/methanol/acetic acid

Retention time (min)

22.89

Amount injected

~ 0.3 ng

DOI for method

10.1016/j.aca.2017.11.002

Date acquired

February 2025

Submitters

N.Kampschulte, M.A.Chromik, R.Kirchhoff, A.Löwen, K.Mosel, K.Plitzko, L.Scholz, L.Wende, M.Wiebel, N.H.Schebb

Source of standard

Cayman Chemical

(+/-)14,15-EpETrE-d11

LMFA03080028

Thermo QExactive HF

[M-H]-

N.Kampschulte, M.A.Chromik, R.Kirchhoff, A.Löwen, K.Mosel, K.Plitzko, L.Scholz, L.Wende, M.Wiebel, N.H.Schebb

Run GNPS MASST Search

Structure

Common name

(+/-)14,15-EpETrE-d11

LMID

LMFA03080028

Systematic name

14,15-epoxy-5Z,8Z,11Z-eicosatrienoic-16,16,17,17,18,18,19,19,20,20,20-d11 acid

m/z

330.3

Adduct / Ion

[M-H]-

Charge

-1

Ion source

HESI

Instrument

Thermo QExactive HF

Resolution

Not Available

Ion mode

Negative

Collision Energy (CE)

30

Column

Zorbax Eclipse Plus C18 reversed phase column (2.1 x 150 mm, particle size 1.8 mm; Agilent, Waldbronn, Germany)

Mobile phase A

95/5 (v/v) 0.1% acetic acid (aq)/Eluent B

Mobile phase B

800/150/1 (v/v/v) acetonitrile/methanol/acetic acid

Retention time (min)

22.89

Amount injected

~ 0.3 ng

DOI for method

10.1016/j.aca.2017.11.002

Date acquired

February 2025

Submitters

N.Kampschulte, M.A.Chromik, R.Kirchhoff, A.Löwen, K.Mosel, K.Plitzko, L.Scholz, L.Wende, M.Wiebel, N.H.Schebb

Source of standard

Cayman Chemical