Common Name
LM ID
Instrument
Adduct / Ion
Submitter
Structure
Common name
25-hydroxy-cholesterol
LMID
Systematic name
cholest-5-en-3beta,25-diol
Adduct / Ion
[M+NH4]+
Charge
1
Ion source
ESI
Instrument
SciEx
Ion mode
Positive
Source of standard
Cayman Chemical
25-hydroxy-cholesterol
Thermo Scientific Orbitrap Exploris 240
[M+H-H2O]+
Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula
[{"mz":71.08562469,"intensity":3225.0910644531},{"mz":80.94775391,"intensity":2404.3557128906},{"mz":89.64634705,"intensity":1413.8120117188},{"mz":95.08559418,"intensity":3211.6677246094},{"mz":107.0856552,"intensity":1886.3247070313},{"mz":109.0146637,"intensity":1465.5968017578},{"mz":109.1011505,"intensity":2687.6232910156},{"mz":111.9040604,"intensity":1362.1346435547},{"mz":133.1011963,"intensity":4638.59765625},{"mz":135.116684,"intensity":3481.1943359375},{"mz":137.1324463,"intensity":1634.5872802734},{"mz":142.553009,"intensity":1504.8687744141},{"mz":143.0070801,"intensity":1631.4989013672},{"mz":143.0267487,"intensity":1573.4698486328},{"mz":145.1014252,"intensity":2896.4968261719},{"mz":147.1173401,"intensity":2665.8923339844},{"mz":149.1323242,"intensity":3561.6735839844},{"mz":151.111618,"intensity":4135.2045898438},{"mz":159.1168671,"intensity":4624.5083007813},{"mz":161.1327362,"intensity":5525.2236328125},{"mz":173.1323853,"intensity":2318.0673828125},{"mz":175.1483612,"intensity":1745.6838378906},{"mz":177.127182,"intensity":2377.3771972656},{"mz":180.6002045,"intensity":1433.7431640625},{"mz":187.1484375,"intensity":4647.4794921875},{"mz":193.1954803,"intensity":1500.2607421875},{"mz":203.1789398,"intensity":2827.5769042969},{"mz":213.1632538,"intensity":2137.7060546875},{"mz":215.1796875,"intensity":3934.8996582031},{"mz":231.1753693,"intensity":2042.3188476563},{"mz":233.2264099,"intensity":3456.0053710938},{"mz":241.1955719,"intensity":2420.5549316406},{"mz":242.7384644,"intensity":1635.8377685547},{"mz":247.2420044,"intensity":1683.3564453125},{"mz":255.210968,"intensity":2877.3833007813},{"mz":257.2263489,"intensity":1688.0643310547},{"mz":273.2213135,"intensity":2313.1550292969},{"mz":285.2588501,"intensity":2042.3195800781},{"mz":291.7832947,"intensity":1444.5914306641},{"mz":311.2744751,"intensity":2535.9770507813},{"mz":324.3268127,"intensity":4505.9047851563},{"mz":367.3361206,"intensity":55820.375},{"mz":385.3468628,"intensity":98038.359375},{"mz":385.3726501,"intensity":3431.8774414063}]
Structure
Common name
25-hydroxy-cholesterol
LMID
Systematic name
cholest-5-en-3beta,25-diol
m/z
385.3465
Adduct / Ion
[M+H-H2O]+
Charge
1
Ion source
HESI
Instrument
Thermo Scientific Orbitrap Exploris 240
Resolution
30000
For high resolution data only
Ion mode
Positive
Collision Energy (CE)
10,20,30
Column
Accucore C30 150 x 2.2
Mobile phase A
Acetonitrile:water 50/50 v/v 5mM AmFm + 0.1% FA
Mobile phase B
Isopropanol:Acetonitrile:water 85/10/5 v/v 5mM AmFm + 0.1% FA
Retention time (min)
6.83
DOI for method
Date acquired
December 2025
Submitters
Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula
Source of standard
Cayman Chemical
25-hydroxy-cholesterol
Thermo Scientific Orbitrap Exploris 240
[M+H-H2O]+
Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula
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Structure
Common name
25-hydroxy-cholesterol
LMID
Systematic name
cholest-5-en-3beta,25-diol
m/z
385.3465
Adduct / Ion
[M+H-H2O]+
Charge
1
Ion source
HESI
Instrument
Thermo Scientific Orbitrap Exploris 240
Resolution
30000
For high resolution data only
Ion mode
Positive
Collision Energy (CE)
20,30,40
Column
Accucore C30 150 x 2.2
Mobile phase A
Acetonitrile:water 50/50 v/v 5mM AmFm + 0.1% FA
Mobile phase B
Isopropanol:Acetonitrile:water 85/10/5 v/v 5mM AmFm + 0.1% FA
Retention time (min)
6.83
DOI for method
Date acquired
December 2025
Submitters
Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula
Source of standard
Cayman Chemical
25-hydroxy-cholesterol
Thermo Scientific Orbitrap Exploris 240
[M+H-H2O]+
Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula
[{"mz":55.05421066,"intensity":2390.3952636719},{"mz":55.84111404,"intensity":1592.5798339844},{"mz":57.06990051,"intensity":3981.0068359375},{"mz":60.14168549,"intensity":1348.2255859375},{"mz":67.05433655,"intensity":1836.5893554688},{"mz":68.75967407,"intensity":1420.5493164063},{"mz":69.06994629,"intensity":8607.140625},{"mz":69.8429184,"intensity":1297.2045898438},{"mz":71.08560181,"intensity":2972.7275390625},{"mz":80.94780731,"intensity":2182.2512207031},{"mz":81.07004547,"intensity":9682.02734375},{"mz":82.06941223,"intensity":1559.64453125},{"mz":83.08557892,"intensity":3804.7465820313},{"mz":85.17984772,"intensity":1483.5902099609},{"mz":91.05412292,"intensity":1322.2702636719},{"mz":93.06996155,"intensity":3119.4108886719},{"mz":95.08548737,"intensity":9486.5302734375},{"mz":97.10140991,"intensity":3066.0161132813},{"mz":98.77398682,"intensity":1346.5546875},{"mz":105.0701675,"intensity":3844.125},{"mz":107.0855179,"intensity":8536.8115234375},{"mz":109.1010742,"intensity":8150.2211914063},{"mz":111.1168747,"intensity":2307.4453125},{"mz":121.1013489,"intensity":5529.5581054688},{"mz":123.1170731,"intensity":3620.3889160156},{"mz":131.0856628,"intensity":3004.9760742188},{"mz":133.1009979,"intensity":10355.19921875},{"mz":135.1170502,"intensity":5023.189453125},{"mz":137.13237,"intensity":2251.0002441406},{"mz":145.1012878,"intensity":5902.1376953125},{"mz":146.2234192,"intensity":1480.7259521484},{"mz":147.1169739,"intensity":9247.9169921875},{"mz":149.1325378,"intensity":4114.2075195313},{"mz":159.1166534,"intensity":10046.0166015625},{"mz":161.1326904,"intensity":8803.2119140625},{"mz":163.1482544,"intensity":2870.1879882813},{"mz":173.1324158,"intensity":5103.5063476563},{"mz":175.1481018,"intensity":1826.5599365234},{"mz":185.1332092,"intensity":1691.6369628906},{"mz":187.147934,"intensity":3308.1784667969},{"mz":199.1481476,"intensity":5555.12890625},{"mz":201.164032,"intensity":2563.0302734375},{"mz":203.1792603,"intensity":1722.0152587891},{"mz":208.2959747,"intensity":1570.8569335938},{"mz":213.1640625,"intensity":5525.1489257813},{"mz":215.1796112,"intensity":3945.3957519531},{"mz":227.180069,"intensity":2578.7221679688},{"mz":233.226059,"intensity":3181.6325683594},{"mz":241.1957703,"intensity":2945.0793457031},{"mz":255.2108307,"intensity":4171.2744140625},{"mz":285.2585754,"intensity":1589.5479736328},{"mz":324.326416,"intensity":7659.1713867188},{"mz":367.335968,"intensity":21438.5},{"mz":385.3470459,"intensity":7044.3432617188},{"mz":385.5367737,"intensity":1466.8056640625}]
Structure
Common name
25-hydroxy-cholesterol
LMID
Systematic name
cholest-5-en-3beta,25-diol
m/z
385.3465
Adduct / Ion
[M+H-H2O]+
Charge
1
Ion source
HESI
Instrument
Thermo Scientific Orbitrap Exploris 240
Resolution
30000
For high resolution data only
Ion mode
Positive
Collision Energy (CE)
30,40,50
Column
Accucore C30 150 x 2.2
Mobile phase A
Acetonitrile:water 50/50 v/v 5mM AmFm + 0.1% FA
Mobile phase B
Isopropanol:Acetonitrile:water 85/10/5 v/v 5mM AmFm + 0.1% FA
Retention time (min)
6.83
DOI for method
Date acquired
December 2025
Submitters
Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula
Source of standard
Cayman Chemical