Ruscogenin is a steroid sapogenin that has been found in O. japonicus with diverse biological activities.^1,2,3,4,5 It reduces adhesion of HL-60 cells to ECV304 cells induced by phorbol 12-myristate 13-acetate (PMA) with an IC50 value of 7.76 nM and decreases peritoneal leukocyte migration induced by zymosan A in mice when administered at a dose of 5 mg/kg.¹ Oral administration of ruscogenin (12.5 mg/kg) inhibits ADP-induced platelet aggregation in rats.² Ruscogenin prevents monocrotaline-induced increases in mean arterial pressure (MAP), expression of IL-1β and TNF in plasma and lung, and endothelial cell apoptosis in pulmonary arterioles in a rat model of pulmonary hypertension.³ It also reduces proteinuria and renal macrophage infiltration in a rat model of diabetes induced by streptozotocin and decreases gene expression of inflammatory cytokines, dyslipidemia, and liver steatosis in a hamster model of high-fat diet-induced nonalcoholic steatohepatitis (NASH).^4,5