Microarray data for Asah1: fold-change ratios

Bone marrow-derived macrophage cells treated with Kdo2-Lipid A (KLA) and ATP

Gene Time course graph Description Treatment 15min 30min 1h 2h 4h 8h 20h
Asah1
KLA  ATP  ATP+KLA
 N-acylsphingosine amidohydrolase 1 (Asah1), mRNA [NM_019734] KLA .80 .84 .84 .69 .72 .78 1.09
 ATP 1.04 1.26 1.00 1.15 .82 .53 .72
 ATP+KLA .87 .89 .63 .82 .51 .47 .49

Bone marrow-derived macrophage cells treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

Gene Time course graph Description Treatment 30min 1h 2h 4h 8h 12h 24h
Asah1
25OHC/Ctl - treatment with 25-OHC / no treatment
25-OHC*/* - lipid depleted media containing compactin and treatment with 25-OHC / lipid depleted media containing compactin only
N-acylsphingosine amidohydrolase 1 (Asah1), mRNA [NM_019734] 25OHC/Ctl (?) .98 .93 1.03 .80 .95 .99 .76
 25-OHC*/* (?) 1.15 .92 .96 .76 .87 .85 .56
 
 

Thioglycolate-elicited macrophage cells treated with Kdo2-Lipid A (KLA) and compactin

Gene Time course graph Description Treatment 30min 1h 2h 4h 8h 12h 24h
Asah1
KLA  Compactin  Compactin+KLA
 N-acylsphingosine amidohydrolase 1 (Asah1), mRNA [NM_019734] KLA 1.10 1.01 .90 .66 .54 .58 .72
 Compactin .93 .98 1.07 .96 .98 1.01 1.07
 Compactin+KLA 1.04 .99 .91 .63 .58 .60 .72

RAW 264.7 cells treated with Kdo2-Lipid A (KLA) and compactin

Gene Time course graph Description Treatment 30min 1h 2h 4h 8h 12h 24h
Asah1
KLA  Compactin  Compactin+KLA
 N-acylsphingosine amidohydrolase 1 (Asah1), mRNA [NM_019734] KLA 1.19 .93 1.00 .93 .88 1.01 1.10
 Compactin 1.12 .97 1.08 1.09 1.05 .90 .89
 Compactin+KLA 1.17 .98 .94 .91 .87 1.02 1.07