Microarray data for Sphk1: fold-change ratios

Bone marrow-derived macrophage cells treated with Kdo2-Lipid A (KLA) and ATP

Gene Time course graph Description Treatment 15min 30min 1h 2h 4h 8h 20h
Sphk1
KLA  ATP  ATP+KLA
 sphingosine kinase 1 (Sphk1), transcript variant 2, mRNA [NM_025367] KLA 2.61 2.50 2.04 1.71 1.19 .91 .74
 ATP .94 1.00 2.06 8.09 16.52 12.59 3.53
 ATP+KLA 2.64 2.81 3.50 8.13 23.71 16.46 2.22

Bone marrow-derived macrophage cells treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

Gene Time course graph Description Treatment 30min 1h 2h 4h 8h 12h 24h
Sphk1
25OHC/Ctl - treatment with 25-OHC / no treatment
25-OHC*/* - lipid depleted media containing compactin and treatment with 25-OHC / lipid depleted media containing compactin only
sphingosine kinase 1 (Sphk1), transcript variant 2, mRNA [NM_025367] 25OHC/Ctl (?) .98 .99 1.02 1.24 1.22 1.29 1.51
 25-OHC*/* (?) .96 1.05 .99 1.16 1.27 1.40 1.39
 
 

Thioglycolate-elicited macrophage cells treated with Kdo2-Lipid A (KLA) and compactin

Gene Time course graph Description Treatment 30min 1h 2h 4h 8h 12h 24h
Sphk1
KLA  Compactin  Compactin+KLA
 sphingosine kinase 1 (Sphk1), transcript variant 2, mRNA [NM_025367] KLA .99 1.12 3.05 4.12 1.23 .70 .60
 Compactin 1.02 1.05 1.16 1.05 1.08 1.01 1.09
 Compactin+KLA .95 1.20 2.83 4.09 1.28 .73 .69

RAW 264.7 cells treated with Kdo2-Lipid A (KLA) and compactin

Gene Time course graph Description Treatment 30min 1h 2h 4h 8h 12h 24h
Sphk1
KLA  Compactin  Compactin+KLA
 sphingosine kinase 1 (Sphk1), transcript variant 2, mRNA [NM_025367] KLA 1.02 .97 1.09 1.25 1.12 1.01 .98
 Compactin .98 .99 .98 .98 1.02 .99 1.04
 Compactin+KLA .98 .98 1.07 1.02 1.08 1.12 1.07