Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: 4beta-hydroxy-cholesterol

Legend:graph_icon: View detailed data for replicatesgraph_icon: View time course graphs
Ctrl:Untreated BMDM cells (control)
25-OHC: Cells treated with 25-OH-cholesterol at time 0
*: BMDM cells in lipid-depleted media containing compactin
25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0
1.190.243 0.203 0.25 0.203 0.23 0.237 0.253 1.02 0.18 0.237 0.243 0.237 0.203 0.21 0.187 1.72 1.85 1.84 1.71 1.77 1.7 1.54 1.25 0.447 0.48 0.547 0.403 0.463 0.463 0.583 0.57 0.313 0.303 0.357 0.437 0.42 0.437 0.41 0.58 0.27 0.373 0.307 0.247 0.34 0.277 0.33 0.377 0.15 0.19 0.2 0.15 0.13 0.13 0.07 0.13 0.13 0.13 0.13 0.12 0.13 0.1 0.12 0.11 0.11 0.08 0.1 0.12 0.1 0.07 0.09 0.11 0.788 0.74 0.615 0.709 0.739 0.555 0.562 0.718 0.891 0.782 0.841 0.513 0.628 0.554 0.595 0.526 0.587 0.513 0.511 0.494 0.509 0.436 0.492 0.466 0.398 0.529 0.332 0.533 0.321 0.34 0.489 0.401 0.328 0.396 0.316 0.317 0.394 0.437 0.58 0.477 0.214 0.26 0.168 0.216 0.247 0.194 0.217 0.127 0.256 0.214 0.21 0.177 0.211 0.159 0.215 0.158 0.225 0.179 0.149 0.122 0.14 0.128 0.176 0.153 0.195 0.18 0.131 0.134 0.149 0.147 0.182 0.138 0.163 0.13 0.127 - - - 0.093 0.08 0.077 0.002 0.002 0.002 0.035 0.032 0.032 0.041 0.039 0.017 0.019 0.024 0.036 0.049 0.028 0.023 0.04 0.019 0.027 0.035 0.042 0.043 0.042 0.039 0.049 0.044 0.032 0.05 0.181 0.138 0.167 0.131 0.124 0.094 0.061 0.072 0.011 0.006 0.015 8.71 4.62 3.67 9.41 4.98 4.06 3.34 3.2 3.17 3.23 4.08 4.46 4.22 3.77 0.828 0.911 1.52 0.845 0.659 0.734 0.612 0.519 0.593 0.932 0.822 0.608 0.485 0.646 0.819 0.773 0.604 0.577 0.663 0.87 0.673 0.596 0.521 0.721 0.54 0.49 0.322 0.719 0.786 0.592 0.497 2.58 0.978 0.611 0.82 0.621 0.553 0.398 0.795 0.372 0.385 0.944 0.51 0.92 0.349 0.492 1.1 0.376 50.4 52.7 79.8 53.9 59.2 28.2 30.8 68.6 86.2 70.3 62.1 29 29.8 92.7 31.3 51.8 103 110
Details Graph
Individual
Expts		Ave Biol. Reps
LM_ID Name 25-OHC*
*
25-OHC
Ctrl
 0 hrs		 25-OHC*
*
25-OHC
Ctrl
 30 min		 25-OHC*
*
25-OHC
Ctrl
 1 hr		 25-OHC*
*
25-OHC
Ctrl
 2 hrs		 25-OHC*
*
25-OHC
Ctrl
 4 hrs		 25-OHC*
*
25-OHC
Ctrl
 8 hrs		 25-OHC*
*
25-OHC
Ctrl
 12 hrs		 25-OHC*
*
25-OHC
Ctrl
 24 hrs		Units
. . . .
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ070605)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ070612)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ070820)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ071011)		LMST01010014 4beta-hydroxy-cholesterol pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ070726)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ070808)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ070823)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ080212)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ080219)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ080226)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD080211)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD080311)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD080408)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090716)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090716)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090805)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090805)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01010014 4beta-hydroxy-cholesterol -
0.18
-
0.17		 0.17
0.18
0.16
0.3		 0.14
0.15
0.17
0.17		 0.23
0.21
0.12
0.15		 0.25
0.21
0.23
0.19		 0.17
0.17
0.14
0.13		 0.19
0.23
0.15
0.13		 0.16
0.21
0.17
0.18		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01010014 4beta-hydroxy-cholesterol -
0.36
-
0.33		 0.34
0.3
0.35
0.26		 0.31
0.23
0.31
0.25		 0.35
0.24
0.25
0.29		 0.33
0.43
0.34
0.42		 0.27
0.26
0.26
0.29		 0.3
0.26
0.29
0.25		 0.25
0.28
0.3
0.33		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01010014 4beta-hydroxy-cholesterol -
0.36
-
0.51		 0.36
0.47
0.56
0.45		 0.39
0.41
0.55
0.44		 0.39
0.4
0.56
0.46		 0.41
0.44
0.78
0.75		 0.47
0.45
0.46
0.64		 0.59
0.74
0.78
0.57		 0.54
0.61
0.88
0.87		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01010014 4beta-hydroxy-cholesterol -
1.76
-
1.74		 1.92
1.87
1.8
2		 1.72
1.77
1.81
1.89		 1.82
1.87
1.72
1.84		 1.79
1.73
1.78
1.72		 1.85
1.9
1.9
1.97		 1.88
1.91
2.06
1.95		 1.89
1.8
1.73
2.02		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01010014 4beta-hydroxy-cholesterol -
3.52
-
3.38		 3.84
3.12
3.93
1.73		 3.8
2.72
3.3
2.79		 2.77
2.13
3.59
3.55		 2.37
3.54
2.64
3.8		 2.94
2.9
3.54
4.39		 2.96
2.16
3.99
3.75		 2.95
2.39
3.05
3.7		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01010014 4beta-hydroxy-cholesterol -
3.52
-
5.22		 4.07
4.88
6.29
3		 4.78
4.85
5.86
4.9		 3.08
3.55
8.05
5.63		 2.94
3.63
6.05
6.79		 5.11
5.02
6.26
9.69		 5.83
6.14
10.7
8.55		 6.36
5.22
8.94
9.76		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070515)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070619)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070724)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ050725)		LMST01010014 4beta-hydroxy-cholesterol ug/extract
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ060926)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ061017)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ061024)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ061024)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ061010)		LMST01010014 4beta-hydroxy-cholesterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ070716)		LMST01010014 4beta-hydroxy-cholesterol pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ070927)		LMST01010014 4beta-hydroxy-cholesterol pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ070927)		LMST01010014 4beta-hydroxy-cholesterol pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ070927)		LMST01010014 4beta-hydroxy-cholesterol pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJSUB002)		LMST01010014 4beta-hydroxy-cholesterol pmol/mg protein
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCISUB300)		 4beta-hydroxy-cholesterol pmol/mg protein
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD110605)		LMST01010014 4beta-hydroxy-cholesterol umol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD110605)		LMST01010014 4beta-hydroxy-cholesterol umol/ml of media
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120120)		LMST01010014 4beta-hydroxy-cholesterol pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120131)		LMST01010014 4beta-hydroxy-cholesterol pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120212)		LMST01010014 4beta-hydroxy-cholesterol pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120308)		LMST01010014 4beta-hydroxy-cholesterol pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(NASH0018)		LMST01010014 4beta-hydroxy-cholesterol pmol/mg
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(NASH0010)		LMST01010014 4beta-hydroxy-cholesterol pmol/ml