Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: C26 GlcCer Legend: : View detailed data for replicates: View time course graphs Ctrl: Untreated BMDM cells (control) 25-OHC: Cells treated with 25-OH-cholesterol at time 0 *: BMDM cells in lipid-depleted media containing compactin 25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0 0.0830.0470.0120.0940.0950.1010.1130.1310.1450.0830.3190.1540.2290.2050.3120.3430.1890.2430.3090.5020.1810.2290.2560.1320.0270.017 0.01 0.107 0.063 0.03 0.067 0.043 0.01 0.01 - - 0.724 0.03 - 0.08 0.03 0.06 0.04 0.02 - 0.09 0.08 - 0.07 0.07 - - - 0.13 0.08 0.03 - - 0.06 0.02 0.09 0.03 - - 0.02 0.01 0.01 0.01 0.01 0.01 0.04 0.02 0.04 0.02 0.01 0.04 0.02 0.04 0.02 - 0.03 0.03 - 0.03 0.03 0.053 0.145 - 0.166 0.043 0.073 0.059 0.049 0.332 0.825 - 0.752 0.402 0.714 0.532 0.443 - 0.014 0.023 0.007 0.02 0.008 0.014 0.02 0.026 0.073 - 0.083 0.022 0.037 0.029 0.024 0.022 0.054 - 0.049 0.027 0.047 0.035 0.029 0.017 0.059 0.114 0.037 - - 0.028 0.013 0.021 0.049 0.143 0.03 - - 0.027 0.016 0.02 0.008 0.012 0.027 0.037 0.007 0.002 0.005 0.017 0.008 0.01 0.03 0.039 0.008 0.003 0.004 0.497 0.629 Details Graph Individual Expts Ave Biol. Reps LM_ID Name 25-OHC* * 25-OHC Ctrl 0 hrs 25-OHC* * 25-OHC Ctrl 30 min 25-OHC* * 25-OHC Ctrl 1 hr 25-OHC* * 25-OHC Ctrl 2 hrs 25-OHC* * 25-OHC Ctrl 4 hrs 25-OHC* * 25-OHC Ctrl 8 hrs 25-OHC* * 25-OHC Ctrl 12 hrs 25-OHC* * 25-OHC Ctrl 24 hrs Units . . . . Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCI060111) LMSP0501AA11 C26 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCI060122) LMSP0501AA11 C26 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCI060126) LMSP0501AA11 C26 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCI060614) LMSP0501AA11 C26 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ060718) LMSP0501AA11 C26 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070123) LMSP0501AA11 C26 GlcCer pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG070312) LMSP0501AA11 C26 GlcCer pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG061213) LMSP0501AA11 C26 GlcCer pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070619) LMSP0501AA11 C26 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070515) LMSP0501AA11 C26 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070515) LMSP0501AA11 C26 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD070716) LMSP0501AA11 C26 GlcCer pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD070815) LMSP0501AA11 C26 GlcCer pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCISUB000) LMSP0501AA11 C26 GlcCer pmol/mg protein Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080212) LMSP0501AA11 C26 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080219) LMSP0501AA11 C26 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080226) LMSP0501AA11 C26 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090127) LMSP0501AA11 C26 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090114) LMSP0501AA11 C26 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090401) LMSP0501AA11 C26 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP0501AA11 C26 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP0501AA11 C26 GlcCer pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ081209) LMSP0501AA11 C26 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP0501AA11 C26 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP0501AA11 C26 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090716) LMSP0501AA11 C26 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090716) LMSP0501AA11 C26 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090805) LMSP0501AA11 C26 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090805) LMSP0501AA11 C26 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMSP0501AA11 C26 GlcCer - - - 0.01 0.01 0.01 0.01 0.01 - - - - 0.01 - - - 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 - 0.01 - 0.01 - 0.01 0.01 - pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) LMSP0501AA11 C26 GlcCer - 0.1 - 0.03 0.03 0.03 0.03 0.02 0.02 0.02 0.02 0.02 0.03 0.02 0.02 0.02 0.05 0.04 0.04 0.04 0.01 0.02 0.02 0.07 0.03 0.02 0.02 0.02 0.04 0.02 0.04 0.02 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) LMSP0501AA11 C26 GlcCer - 0.03 - 0.04 0.03 0.07 0.06 0.03 0.03 0.03 0.07 0.04 0.04 0.04 0.03 0.04 0.05 0.03 0.1 0.06 0.06 0.05 0.09 0.08 0.09 0.1 0.16 0.09 0.07 0.08 0.29 0.16 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMSP0501AA11 C26 GlcCer - - - 0.1 0.11 0.1 0.11 0.07 - - - - 0.08 - - - 0.07 0.08 0.08 0.09 0.11 0.11 0.14 0.15 - 0.08 - 0.15 - 0.09 0.1 - pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) LMSP0501AA11 C26 GlcCer - 0.74 - 0.28 0.25 0.27 0.27 0.22 0.19 0.2 0.2 0.23 0.23 0.22 0.24 0.22 0.39 0.26 0.37 0.28 0.1 0.21 0.24 0.73 0.25 0.19 0.21 0.28 0.4 0.19 0.43 0.2 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) LMSP0501AA11 C26 GlcCer - 0.24 - 0.3 0.22 0.4 0.36 0.21 0.22 0.19 0.46 0.29 0.28 0.26 0.17 0.31 0.34 0.2 0.45 0.28 0.41 0.33 0.68 0.45 0.46 0.46 0.86 0.64 0.37 0.39 1.34 0.83 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD110605) LMSP05010011 C26 GlcCer pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070122) LMSP0501AA11 C26 GlcCer pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070124) LMSP0501AA11 C26 GlcCer pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCISUB300) LMSP0501AA11 C26 GlcCer pmol/mg protein