Microarray analysis of RAW 264.7 cells treated with Kdo2-Lipid A (KLA) and compactin (full time course)
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IntroductionExperimental designCompactin is a potent competitive inhibitor of hydroxymethylglutaryl coenzyme A reductase, the rate-limiting enzyme in cholesterol biosynthesis. Experiments were conducted with RAW264.7 cells fed 10% fetal calf serum, as per protocol PP0000002800 (pdf). Measurements were taken at 30m, 1h, 2h, 4h, 8h, 12h, and 24h for: (i) compactin, (ii) Kdo2-Lipid A, (iii) compactin + Kdo2-Lipid A. and (iv) control. Array Design45,214 spots on the array41,034 data spots 38,838 unique probes (6428 probes have no gene id) 21,609 unique mouse genes Each gene was represented by 1 - 9 probes Each probe was spotted 1 or 10 times Data NormalizationData normalization was performed as described in J Mol Endocrinol Sasik et al. 33 (1): 1 (PDF) |
References
Kdo2-Lipid A of Escherichia coli, a defined endotoxin that activates macrophages via TLR-4.
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Journal of Lipid Research 47, 1097-1111 (2006)