Gene/Proteome Database (LMPD)

LMPD ID
LMP002259
Gene ID
5335
Species
Homo sapiens (Human)
Gene Name
phospholipase C, gamma 1
Gene Symbol
PLCG1
Synonyms
NCKAP3; PLC-II; PLC1; PLC148; PLCgamma1
Alternate Names
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; PLC-148; PLC-gamma-1; phospholipase C-II; phospholipase C-148; phosphoinositidase C; phospholipase C-gamma-1; inositoltrisphosphohydrolase; phosphoinositide phospholipase C; phosphatidylinositol phospholipase C; triphosphoinositide phosphodiesterase; phosphoinositide phospholipase C-gamma-1; monophosphatidylinositol phosphodiesterase; 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; phospholipase C, gamma 1 (formerly subtype 148); 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1
Chromosome
20
Map Location
20q12-q13.1
EC Number
3.1.4.11
Summary
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Orthologs
View orthologs and multiple alignments for PLCG1

Proteins

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform a
Refseq ID NP_002651
Protein GI 33598948
UniProt ID P19174
mRNA ID NM_002660
Length 1291
RefSeq Status REVIEWED
MAGAASPCANGCGPGAPSDAEVLHLCRSLEVGTVMTLFYSKKSQRPERKTFQVKLETRQITWSRGADKIEGAIDIREIKEIRPGKTSRDFDRYQEDPAFRPDQSHCFVILYGMEFRLKTLSLQATSEDEVNMWIKGLTWLMEDTLQAPTPLQIERWLRKQFYSVDRNREDRISAKDLKNMLSQVNYRVPNMRFLRERLTDLEQRSGDITYGQFAQLYRSLMYSAQKTMDLPFLEASTLRAGERPELCRVSLPEFQQFLLDYQGELWAVDRLQVQEFMLSFLRDPLREIEEPYFFLDEFVTFLFSKENSVWNSQLDAVCPDTMNNPLSHYWISSSHNTYLTGDQFSSESSLEAYARCLRMGCRCIELDCWDGPDGMPVIYHGHTLTTKIKFSDVLHTIKEHAFVASEYPVILSIEDHCSIAQQRNMAQYFKKVLGDTLLTKPVEISADGLPSPNQLKRKILIKHKKLAEGSAYEEVPTSMMYSENDISNSIKNGILYLEDPVNHEWYPHYFVLTSSKIYYSEETSSDQGNEDEEEPKEVSSSTELHSNEKWFHGKLGAGRDGRHIAERLLTEYCIETGAPDGSFLVRESETFVGDYTLSFWRNGKVQHCRIHSRQDAGTPKFFLTDNLVFDSLYDLITHYQQVPLRCNEFEMRLSEPVPQTNAHESKEWYHASLTRAQAEHMLMRVPRDGAFLVRKRNEPNSYAISFRAEGKIKHCRVQQEGQTVMLGNSEFDSLVDLISYYEKHPLYRKMKLRYPINEEALEKIGTAEPDYGALYEGRNPGFYVEANPMPTFKCAVKALFDYKAQREDELTFIKSAIIQNVEKQEGGWWRGDYGGKKQLWFPSNYVEEMVNPVALEPEREHLDENSPLGDLLRGVLDVPACQIAIRPEGKNNRLFVFSISMASVAHWSLDVAADSQEELQDWVKKIREVAQTADARLTEGKIMERRKKIALELSELVVYCRPVPFDEEKIGTERACYRDMSSFPETKAEKYVNKAKGKKFLQYNRLQLSRIYPKGQRLDSSNYDPLPMWICGSQLVALNFQTPDKPMQMNQALFMTGRHCGYVLQPSTMRDEAFDPFDKSSLRGLEPCAISIEVLGARHLPKNGRGIVCPFVEIEVAGAEYDSTKQKTEFVVDNGLNPVWPAKPFHFQISNPEFAFLRFVVYEEDMFSDQNFLAQATFPVKGLKTGYRAVPLKNNYSEDLELASLLIKIDIFPAKQENGDLSPFSGTSLRERGSDASGQLFHGRAREGSFESRYQQPFEDFRISQEHLADHFDSRERRAPRRTRVNGDNRL
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform b
Refseq ID NP_877963
Protein GI 33598946
UniProt ID P19174
mRNA ID NM_182811
Length 1290
RefSeq Status REVIEWED
MAGAASPCANGCGPGAPSDAEVLHLCRSLEVGTVMTLFYSKKSQRPERKTFQVKLETRQITWSRGADKIEGAIDIREIKEIRPGKTSRDFDRYQEDPAFRPDQSHCFVILYGMEFRLKTLSLQATSEDEVNMWIKGLTWLMEDTLQAPTPLQIERWLRKQFYSVDRNREDRISAKDLKNMLSQVNYRVPNMRFLRERLTDLEQRSGDITYGQFAQLYRSLMYSAQKTMDLPFLEASTLRAGERPELCRVSLPEFQQFLLDYQGELWAVDRLQVQEFMLSFLRDPLREIEEPYFFLDEFVTFLFSKENSVWNSQLDAVCPDTMNNPLSHYWISSSHNTYLTGDQFSSESSLEAYARCLRMGCRCIELDCWDGPDGMPVIYHGHTLTTKIKFSDVLHTIKEHAFVASEYPVILSIEDHCSIAQQRNMAQYFKKVLGDTLLTKPVEISADGLPSPNQLKRKILIKHKKLAEGSAYEEVPTSMMYSENDISNSIKNGILYLEDPVNHEWYPHYFVLTSSKIYYSEETSSDQGNEDEEEPKEVSSSTELHSNEKWFHGKLGAGRDGRHIAERLLTEYCIETGAPDGSFLVRESETFVGDYTLSFWRNGKVQHCRIHSRQDAGTPKFFLTDNLVFDSLYDLITHYQQVPLRCNEFEMRLSEPVPQTNAHESKEWYHASLTRAQAEHMLMRVPRDGAFLVRKRNEPNSYAISFRAEGKIKHCRVQQEGQTVMLGNSEFDSLVDLISYYEKHPLYRKMKLRYPINEEALEKIGTAEPDYGALYEGRNPGFYVEANPMPTFKCAVKALFDYKAQREDELTFIKSAIIQNVEKQEGGWWRGDYGGKKQLWFPSNYVEEMVNPVALEPEREHLDENSPLGDLLRGVLDVPACQIAIRPEGKNNRLFVFSISMASVAHWSLDVAADSQEELQDWVKKIREVAQTADARLTEGKIMERRKKIALELSELVVYCRPVPFDEEKIGTERACYRDMSSFPETKAEKYVNKAKGKKFLQYNRLQLSRIYPKGQRLDSSNYDPLPMWICGSQLVALNFQTPDKPMQMNQALFMTGRHCGYVLQPSTMRDEAFDPFDKSSLRGLEPCAISIEVLGARHLPKNGRGIVCPFVEIEVAGAEYDSTKQKTEFVVDNGLNPVWPAKPFHFQISNPEFAFLRFVVYEEDMFSDQNFLAQATFPVKGLKTGYRAVPLKNNYSEDLELASLLIKIDIFPAKENGDLSPFSGTSLRERGSDASGQLFHGRAREGSFESRYQQPFEDFRISQEHLADHFDSRERRAPRRTRVNGDNRL

Gene Information

Entrez Gene ID
5335
Gene Name
phospholipase C, gamma 1
Gene Symbol
PLCG1
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0008180 IDA:UniProtKB C COP9 signalosome
GO:0042995 IDA:BHF-UCL C cell projection
GO:0005911 IEA:Ensembl C cell-cell junction
GO:0005737 IDA:MGI C cytoplasm
GO:0005829 IDA:UniProtKB C cytosol
GO:0030027 IDA:UniProtKB C lamellipodium
GO:0005886 IDA:UniProtKB C plasma membrane
GO:0001726 IDA:UniProtKB C ruffle
GO:0005509 IEA:InterPro F calcium ion binding
GO:0005168 IPI:UniProtKB F neurotrophin TRKA receptor binding
GO:0004435 IDA:UniProtKB F phosphatidylinositol phospholipase C activity
GO:0004629 TAS:Reactome F phospholipase C activity
GO:0019901 IPI:BHF-UCL F protein kinase binding
GO:0005057 NAS:UniProtKB F receptor signaling protein activity
GO:0038095 TAS:Reactome P Fc-epsilon receptor signaling pathway
GO:0038096 TAS:Reactome P Fc-gamma receptor signaling pathway involved in phagocytosis
GO:0050852 TAS:Reactome P T cell receptor signaling pathway
GO:0000186 TAS:Reactome P activation of MAPKK activity
GO:0007202 TAS:Reactome P activation of phospholipase C activity
GO:0007411 TAS:Reactome P axon guidance
GO:0007596 TAS:Reactome P blood coagulation
GO:0019722 IMP:BHF-UCL P calcium-mediated signaling
GO:0016477 IMP:BHF-UCL P cell migration
GO:0071364 IDA:UniProtKB P cellular response to epidermal growth factor stimulus
GO:0019221 TAS:Reactome P cytokine-mediated signaling pathway
GO:0007173 IMP:BHF-UCL P epidermal growth factor receptor signaling pathway
GO:0008543 TAS:Reactome P fibroblast growth factor receptor signaling pathway
GO:0001701 IEA:Ensembl P in utero embryonic development
GO:0045087 TAS:Reactome P innate immune response
GO:0043647 TAS:Reactome P inositol phosphate metabolic process
GO:0050900 TAS:Reactome P leukocyte migration
GO:0048011 TAS:Reactome P neurotrophin TRK receptor signaling pathway
GO:0009395 IEA:InterPro P phospholipid catabolic process
GO:0045766 IDA:DFLAT P positive regulation of angiogenesis
GO:0043536 IDA:DFLAT P positive regulation of blood vessel endothelial cell migration
GO:0010634 IMP:UniProtKB P positive regulation of epithelial cell migration
GO:0051281 IMP:BHF-UCL P positive regulation of release of sequestered calcium ion into cytosol
GO:0007165 NAS:UniProtKB P signal transduction
GO:0044281 TAS:Reactome P small molecule metabolic process
GO:0016032 IEA:UniProtKB-KW P viral process

KEGG Pathway Links

KEGG Pathway ID Description
hsa04750 Inflammatory mediator regulation of TRP channels
hsa04015 Rap1 signaling pathway
hsa04014 Ras signaling pathway
hsa04919 Thyroid hormone signaling pathway

Domain Information

InterPro Annotations

Accession Description
IPR028380 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
IPR000008 C2 domain
IPR018247 EF-Hand 1, calcium-binding site
IPR002048 EF-hand domain
IPR011992 EF-hand domain pair
IPR017946 PLC-like phosphodiesterase, TIM beta/alpha-barrel domain
IPR016279 Phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma
IPR001192 Phosphoinositide phospholipase C family
IPR000909 Phospholipase C, phosphatidylinositol-specific , X domain
IPR001711 Phospholipase C, phosphatidylinositol-specific, Y domain
IPR015359 Phospholipase C, phosphoinositol-specific, EF-hand-like
IPR001849 Pleckstrin homology domain
IPR011993 Pleckstrin homology-like domain
IPR000980 SH2 domain
IPR001452 SH3_domain

UniProt Annotations

Entry Information

Gene Name
phospholipase C, gamma 1
Protein Entry
PLCG1_HUMAN
UniProt ID
P19174
Species
Human

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P19174-1; Sequence=Displayed; Name=2; IsoId=P19174-2; Sequence=VSP_038692; Note=Contains a phosphoserine at position 1222.;
Catalytic Activity 1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.
Cofactor Name=Ca(2+); Xref=ChEBI
Domain The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
Enzyme Regulation Activated by phosphorylation on tyrosine residues.
Function Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand- mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration.
Interaction P42684:ABL2; NbExp=4; IntAct=EBI-79387, EBI-1102694; P31749:AKT1; NbExp=9; IntAct=EBI-79387, EBI-296087; P10275:AR; NbExp=22; IntAct=EBI-79387, EBI-608057; Q9ULH1:ASAP1; NbExp=3; IntAct=EBI-79387, EBI-346622; O43150:ASAP2; NbExp=3; IntAct=EBI-79387, EBI-310968; P20273:CD22; NbExp=2; IntAct=EBI-79387, EBI-78277; Q9H1R2:DUSP15; NbExp=2; IntAct=EBI-79387, EBI-1752795; Q71V39:EEF1A2 (xeno); NbExp=3; IntAct=EBI-79387, EBI-7645815; P00533:EGFR; NbExp=6; IntAct=EBI-79387, EBI-297353; P04626:ERBB2; NbExp=5; IntAct=EBI-79387, EBI-641062; P21860:ERBB3; NbExp=4; IntAct=EBI-79387, EBI-720706; P31994:FCGR2B; NbExp=2; IntAct=EBI-79387, EBI-724784; P11362:FGFR1; NbExp=5; IntAct=EBI-79387, EBI-1028277; P17948:FLT1; NbExp=2; IntAct=EBI-79387, EBI-1026718; Q13480:GAB1; NbExp=36; IntAct=EBI-79387, EBI-517684; P62993:GRB2; NbExp=2; IntAct=EBI-79387, EBI-401755; P08631:HCK; NbExp=2; IntAct=EBI-79387, EBI-346340; P06213:INSR; NbExp=9; IntAct=EBI-79387, EBI-475899; Q07666:KHDRBS1; NbExp=2; IntAct=EBI-79387, EBI-1364; P10721:KIT; NbExp=31; IntAct=EBI-79387, EBI-1379503; O43561:LAT; NbExp=6; IntAct=EBI-79387, EBI-1222766; Q13094:LCP2; NbExp=3; IntAct=EBI-79387, EBI-346946; Q92918:MAP4K1; NbExp=6; IntAct=EBI-79387, EBI-881; P08581:MET; NbExp=10; IntAct=EBI-79387, EBI-1039152; P09619:PDGFRB; NbExp=5; IntAct=EBI-79387, EBI-641237; P63000:RAC1; NbExp=7; IntAct=EBI-79387, EBI-413628; P07949:RET; NbExp=2; IntAct=EBI-79387, EBI-2480756; Q8TB24:RIN3; NbExp=3; IntAct=EBI-79387, EBI-1570523; Q14108:SCARB2; NbExp=2; IntAct=EBI-79387, EBI-1564650; Q9UPX8:SHANK2; NbExp=4; IntAct=EBI-79387, EBI-1570571; Q9BYB0:SHANK3; NbExp=2; IntAct=EBI-79387, EBI-1752330; Q15036:SNX17; NbExp=2; IntAct=EBI-79387, EBI-1752620; Q07889:SOS1; NbExp=3; IntAct=EBI-79387, EBI-297487; Q07890:SOS2; NbExp=4; IntAct=EBI-79387, EBI-298181; P43405:SYK; NbExp=4; IntAct=EBI-79387, EBI-78302; P09327:VIL1; NbExp=5; IntAct=EBI-79387, EBI-746958;
Ptm Tyrosine phosphorylated in response to signaling via activated FLT3, KIT and PDGFRA (By similarity). Tyrosine phosphorylated by activated FGFR1, FGFR2, FGFR3 and FGFR4. Tyrosine phosphorylated by activated FLT1 and KDR. Tyrosine phosphorylated by activated PDGFRB. The receptor-mediated activation of PLCG1 involves its phosphorylation by tyrosine kinases, in response to ligation of a variety of growth factor receptors and immune system receptors. For instance, SYK phosphorylates and activates PLCG1 in response to ligation of the B-cell receptor. May be dephosphorylated by PTPRJ. Phosphorylated by ITK and TXK on Tyr-783 upon TCR activation in T-cells. {ECO
Ptm Ubiquitinated by CBLB in activated T-cells.
Similarity Contains 1 C2 domain. {ECO
Similarity Contains 1 EF-hand domain. {ECO
Similarity Contains 1 PI-PLC X-box domain. {ECO
Similarity Contains 1 PI-PLC Y-box domain. {ECO
Similarity Contains 1 SH3 domain. {ECO
Similarity Contains 2 PH domains. {ECO
Similarity Contains 2 SH2 domains. {ECO
Subcellular Location Cell projection, lamellipodium. Cell projection, ruffle. Note=Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
Subunit Interacts with AGAP2 via its SH3 domain. Interacts (via SH2 domain) with RET. Interacts with FLT1 (tyrosine- phosphorylated) (By similarity). Interacts (via SH2 domain) with FGFR1, FGFR2, FGFR3 and FGFR4 (phosphorylated). Interacts with LAT (phosphorylated) upon TCR activation. Interacts (via SH3 domain) with the Pro-rich domain of TNK1. Associates with BLNK, VAV1, GRB2 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with CBLB in activated T-cells; which inhibits phosphorylation. Interacts with SHB. Interacts (via SH3 domain) with the Arg/Gly- rich-flanked Pro-rich domains of KHDRBS1/SAM68. This interaction is selectively regulated by arginine methylation of KHDRBS1/SAM68. Interacts with INPP5D/SHIP1, THEMIS and CLNK (By similarity). Interacts with AXL, FLT4 and KIT. Interacts with RALGPS1. Interacts (via SH3 domain) with HEV ORF3 protein. Interacts (via the SH2 domains) with VIL1 (phosphorylated at C-terminus tyrosine phosphorylation sites). Interacts (via SH2 domain) with PDGFRA and PDGFRB (tyrosine phosphorylated). Interacts with PIP5K1C (By similarity). Interacts with NTRK1 and NTRK2 (phosphorylated upon ligand-binding). Interacts with SYK; activates PLCG1. Interacts with GRB2, LAT and THEMIS upon TCR activation in thymocytes (By similarity). Interacts with TESPA1; the association is increased with prolonged stimulation of the TCR and may facilitate the assembly of the LAT signalosome. {ECO
Web Resource Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/plcg1/";

Identical and Related Proteins

Unique RefSeq proteins for LMP002259 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
33598948 RefSeq NP_002651 1291 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform a
33598946 RefSeq NP_877963 1290 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform b

Identical Sequences to LMP002259 proteins

Reference Database Accession Length Protein Name
GI:33598946 GenBank AAA36452.1 1290 phospholipase C-gamma [Homo sapiens]
GI:33598946 GenBank AAV20696.1 1290 Sequence 956 from patent US 6753314
GI:33598948 GenBank ABH83636.1 1291 Sequence 78 from patent US 7049076
GI:33598948 GenBank EAW75990.1 1291 phospholipase C, gamma 1, isoform CRA_b [Homo sapiens]
GI:33598946 GenBank EAW75991.1 1290 phospholipase C, gamma 1, isoform CRA_c [Homo sapiens]
GI:33598946 GenBank EAW75992.1 1290 phospholipase C, gamma 1, isoform CRA_c [Homo sapiens]
GI:33598948 GenBank ADL96412.1 1291 Sequence 2 from patent US 7718382
GI:33598946 GenBank AED62138.1 1290 Sequence 20 from patent US 7902154
GI:33598948 GenBank AEP52868.1 1291 Sequence 78 from patent US 8017398
GI:33598948 GenBank AHD75940.1 1291 Sequence 18323 from patent US 8586006
GI:33598946 GenBank AHD75941.1 1290 Sequence 18324 from patent US 8586006

Related Sequences to LMP002259 proteins

Reference Database Accession Length Protein Name
GI:33598946 DBBJ BAE06110.1 1412 PLCG1 variant protein, partial [Homo sapiens]
GI:33598946 DBBJ BAG10236.1 1290 phospholipase C, gamma 1, partial [synthetic construct]
GI:33598948 GenBank ABB84466.1 1291 phospholipase C, gamma 1 [Homo sapiens]
GI:33598946 GenBank ABH83636.1 1291 Sequence 78 from patent US 7049076
GI:33598948 GenBank AAI44137.1 1291 Phospholipase C, gamma 1 [Homo sapiens]
GI:33598946 GenBank AAI36467.1 1290 Phospholipase C, gamma 1 [Homo sapiens]
GI:33598948 GenBank JAA15302.1 1291 phospholipase C, gamma 1 [Pan troglodytes]
GI:33598948 GenBank JAA25083.1 1291 phospholipase C, gamma 1 [Pan troglodytes]
GI:33598948 GenBank JAA43582.1 1291 phospholipase C, gamma 1 [Pan troglodytes]
GI:33598948 RefSeq XP_514650.3 1291 PREDICTED: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform X2 [Pan troglodytes]
GI:33598946 RefSeq XP_004062205.1 1290 PREDICTED: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 [Gorilla gorilla gorilla]
GI:33598946 RefSeq XP_009435517.1 1290 PREDICTED: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 isoform X3 [Pan troglodytes]