Gene/Proteome Database (LMPD)

LMPD ID
LMP002282
Gene ID
20423
Species
Mus musculus (Mouse)
Gene Name
sonic hedgehog
Gene Symbol
Shh
Synonyms
9530036O11Rik; Dsh; Hhg1; Hx; Hxl3; M100081
Alternate Names
sonic hedgehog protein; HHG-1; short digits; hemimelic extra toes
Chromosome
5
Map Location
5 B1|5 14.39 cM

Proteins

sonic hedgehog protein precursor
Refseq ID NP_033196
Protein GI 21617861
UniProt ID Q62226
mRNA ID NM_009170
Length 437
RefSeq Status VALIDATED
MLLLLARCFLVILASSLLVCPGLACGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKITRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGGCFPGSATVHLEQGGTKLVKDLRPGDRVLAADDQGRLLYSDFLTFLDRDEGAKKVFYVIETLEPRERLLLTAAHLLFVAPHNDSGPTPGPSALFASRVRPGQRVYVVAERGGDRRLLPAAVHSVTLREEEAGAYAPLTAHGTILINRVLASCYAVIEEHSWAHRAFAPFRLAHALLAALAPARTDGGGGGSIPAAQSATEARGAEPTAGIHWYSQLLYHIGTWLLDSETMHPLGMAVKSS
sig_peptide: 1..24 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 2530 peptide sequence: MLLLLARCFLVILASSLLVCPGLA mat_peptide: 25..437 product: Sonic hedgehog protein experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (Q62226.2) calculated_mol_wt: 45261 peptide sequence: CGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKITRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGGCFPGSATVHLEQGGTKLVKDLRPGDRVLAADDQGRLLYSDFLTFLDRDEGAKKVFYVIETLEPRERLLLTAAHLLFVAPHNDSGPTPGPSALFASRVRPGQRVYVVAERGGDRRLLPAAVHSVTLREEEAGAYAPLTAHGTILINRVLASCYAVIEEHSWAHRAFAPFRLAHALLAALAPARTDGGGGGSIPAAQSATEARGAEPTAGIHWYSQLLYHIGTWLLDSETMHPLGMAVKSS

Gene Information

Entrez Gene ID
20423
Gene Name
sonic hedgehog
Gene Symbol
Shh
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0009986 IDA:MGI C cell surface
GO:0031012 IDA:MGI C extracellular matrix
GO:0005615 IDA:MGI C extracellular space
GO:0016020 IDA:MGI C membrane
GO:0045121 IDA:MGI C membrane raft
GO:0005634 IDA:MGI C nucleus
GO:0005886 IEA:UniProtKB-KW C plasma membrane
GO:0005509 ISS:UniProtKB F calcium ion binding
GO:0001948 IDA:MGI F glycoprotein binding
GO:0005539 IDA:MGI F glycosaminoglycan binding
GO:0043237 IDA:MGI F laminin-1 binding
GO:0005113 IPI:BHF-UCL F patched binding
GO:0008270 ISS:UniProtKB F zinc ion binding
GO:0060020 IDA:MGI P Bergmann glial cell differentiation
GO:0043369 IMP:BHF-UCL P CD4-positive or CD8-positive, alpha-beta T cell lineage commitment
GO:0033077 IMP:BHF-UCL P T cell differentiation in thymus
GO:0048856 IMP:MGI P anatomical structure development
GO:0048646 IMP:MGI P anatomical structure formation involved in morphogenesis
GO:0008209 IMP:MGI P androgen metabolic process
GO:0001525 IDA:MGI P angiogenesis
GO:0009952 IMP:MGI P anterior/posterior pattern specification
GO:0006915 IGI:MGI P apoptotic process
GO:0097190 IDA:Roslin P apoptotic signaling pathway
GO:0060840 IMP:MGI P artery development
GO:0007411 IDA:MGI P axon guidance
GO:0007596 IMP:MGI P blood coagulation
GO:0060442 IDA:MGI P branching involved in prostate gland morphogenesis
GO:0060445 IMP:MGI P branching involved in salivary gland morphogenesis
GO:0001658 IMP:MGI P branching involved in ureteric bud morphogenesis
GO:0048754 IMP:MGI P branching morphogenesis of an epithelial tube
GO:0060447 IMP:MGI P bud outgrowth involved in lung branching
GO:0043010 IDA:MGI P camera-type eye development
GO:0060070 IDA:MGI P canonical Wnt signaling pathway
GO:0048468 IMP:MGI P cell development
GO:0045165 IGI:MGI P cell fate commitment
GO:0001708 IDA:MGI P cell fate specification
GO:0008283 IGI:MGI P cell proliferation
GO:0007267 IMP:MGI P cell-cell signaling
GO:0071285 IDA:MGI P cellular response to lithium ion
GO:0007417 IMP:MGI P central nervous system development
GO:0021930 IGI:UniProtKB P cerebellar granule cell precursor proliferation
GO:0003140 IMP:BHF-UCL P determination of left/right asymmetry in lateral mesoderm
GO:0007368 IMP:MGI P determination of left/right symmetry
GO:0048589 IMP:MGI P developmental growth
GO:0048546 IMP:MGI P digestive tract morphogenesis
GO:0021904 IMP:BHF-UCL P dorsal/ventral neural tube patterning
GO:0009953 IGI:MGI P dorsal/ventral pattern formation
GO:0007398 IMP:MGI P ectoderm development
GO:0048557 TAS:BHF-UCL P embryonic digestive tract morphogenesis
GO:0042733 IMP:Roslin P embryonic digit morphogenesis
GO:0048617 IMP:MGI P embryonic foregut morphogenesis
GO:0035115 IMP:MGI P embryonic forelimb morphogenesis
GO:0035116 IMP:MGI P embryonic hindlimb morphogenesis
GO:0030326 IMP:MGI P embryonic limb morphogenesis
GO:0048598 IMP:MGI P embryonic morphogenesis
GO:0048568 IMP:MGI P embryonic organ development
GO:0048706 IGI:MGI P embryonic skeletal system development
GO:0006897 IDA:MGI P endocytosis
GO:0060664 IMP:MGI P epithelial cell proliferation involved in salivary gland morphogenesis
GO:0060441 IMP:MGI P epithelial tube branching involved in lung morphogenesis
GO:0060684 IMP:MGI P epithelial-mesenchymal cell signaling
GO:0060738 IGI:MGI P epithelial-mesenchymal signaling involved in prostate gland development
GO:0030010 IDA:MGI P establishment of cell polarity
GO:0030900 IGI:MGI P forebrain development
GO:0048859 IMP:MGI P formation of anatomical boundary
GO:0001942 IMP:MGI P hair follicle development
GO:0031069 IMP:MGI P hair follicle morphogenesis
GO:0007507 IMP:MGI P heart development
GO:0001947 IMP:BHF-UCL P heart looping
GO:0030902 IMP:MGI P hindbrain development
GO:0007442 IMP:MGI P hindgut morphogenesis
GO:0048839 IMP:MGI P inner ear development
GO:0016539 IEA:InterPro P intein-mediated protein splicing
GO:0045109 IMP:MGI P intermediate filament organization
GO:0001822 IMP:MGI P kidney development
GO:0060459 IMP:MGI P left lung development
GO:0060174 IMP:MGI P limb bud formation
GO:0060173 IMP:MGI P limb development
GO:0030324 IMP:MGI P lung development
GO:0060428 IMP:MGI P lung epithelium development
GO:0060463 IMP:MGI P lung lobe morphogenesis
GO:0060425 IMP:MGI P lung morphogenesis
GO:0060484 IMP:MGI P lung-associated mesenchyme development
GO:0002320 IMP:BHF-UCL P lymphoid progenitor cell differentiation
GO:0030539 IMP:MGI P male genitalia development
GO:0010463 IMP:MGI P mesenchymal cell proliferation
GO:0060916 IDA:MGI P mesenchymal cell proliferation involved in lung development
GO:0060783 IMP:MGI P mesenchymal smoothened signaling pathway involved in prostate gland development
GO:0072136 IMP:UniProtKB P metanephric mesenchymal cell proliferation involved in metanephros development
GO:0001656 IMP:UniProtKB P metanephros development
GO:0030901 IGI:MGI P midbrain development
GO:0080125 IMP:MGI P multicellular structure septum development
GO:0045445 IDA:MGI P myoblast differentiation
GO:0014902 IMP:MGI P myotube differentiation
GO:0042130 IMP:MGI P negative regulation of T cell proliferation
GO:0030178 IDA:MGI P negative regulation of Wnt signaling pathway
GO:0046639 IMP:MGI P negative regulation of alpha-beta T cell differentiation
GO:0043066 IDA:Roslin P negative regulation of apoptotic process
GO:0090090 IMP:MGI P negative regulation of canonical Wnt signaling pathway
GO:0045596 IDA:MGI P negative regulation of cell differentiation
GO:0030336 IDA:MGI P negative regulation of cell migration
GO:0090370 IMP:BHF-UCL P negative regulation of cholesterol efflux
GO:0010629 IMP:UniProtKB P negative regulation of gene expression
GO:2000357 IDA:UniProtKB P negative regulation of kidney smooth muscle cell differentiation
GO:2001054 IMP:MGI P negative regulation of mesenchymal cell apoptotic process
GO:0032435 IMP:MGI P negative regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0042177 IMP:MGI P negative regulation of protein catabolic process
GO:0034244 IDA:MGI P negative regulation of transcription elongation from RNA polymerase II promoter
GO:0000122 IMP:BHF-UCL P negative regulation of transcription from RNA polymerase II promoter
GO:2000062 IDA:UniProtKB P negative regulation of ureter smooth muscle cell differentiation
GO:0045060 IMP:BHF-UCL P negative thymic T cell selection
GO:0001755 IMP:MGI P neural crest cell migration
GO:0007405 IDA:MGI P neuroblast proliferation
GO:0048663 IMP:MGI P neuron fate commitment
GO:0042476 IDA:MGI P odontogenesis
GO:0042475 IMP:MGI P odontogenesis of dentin-containing tooth
GO:0014003 IDA:MGI P oligodendrocyte development
GO:0048709 IGI:MGI P oligodendrocyte differentiation
GO:0048645 IMP:MGI P organ formation
GO:0002076 IDA:MGI P osteoblast development
GO:0060021 IMP:MGI P palate development
GO:0031016 IMP:MGI P pancreas development
GO:0007389 IMP:MGI P pattern specification process
GO:0001569 IMP:MGI P patterning of blood vessels
GO:0009949 IMP:Roslin P polarity specification of anterior/posterior axis
GO:0033089 IMP:BHF-UCL P positive regulation of T cell differentiation in thymus
GO:0030177 IMP:MGI P positive regulation of Wnt signaling pathway
GO:0046638 IMP:BHF-UCL P positive regulation of alpha-beta T cell differentiation
GO:0045597 IMP:MGI P positive regulation of cell differentiation
GO:0051781 IEA:Ensembl P positive regulation of cell division
GO:0008284 IDA:MGI P positive regulation of cell proliferation
GO:0021940 IDA:MGI P positive regulation of cerebellar granule cell precursor proliferation
GO:0060769 IMP:MGI P positive regulation of epithelial cell proliferation involved in prostate gland development
GO:0010628 IMP:UniProtKB P positive regulation of gene expression
GO:0007228 IMP:BHF-UCL P positive regulation of hh target transcription factor activity
GO:0033092 IMP:BHF-UCL P positive regulation of immature T cell proliferation in thymus
GO:2000358 IDA:UniProtKB P positive regulation of kidney smooth muscle cell differentiation
GO:0002053 IMP:MGI P positive regulation of mesenchymal cell proliferation
GO:2000729 IMP:UniProtKB P positive regulation of mesenchymal cell proliferation involved in ureter development
GO:0002052 IDA:MGI P positive regulation of neuroblast proliferation
GO:0048714 IDA:MGI P positive regulation of oligodendrocyte differentiation
GO:0042307 IGI:MGI P positive regulation of protein import into nucleus
GO:0061189 IEA:Ensembl P positive regulation of sclerotome development
GO:0014858 IMP:MGI P positive regulation of skeletal muscle cell proliferation
GO:0048643 IMP:MGI P positive regulation of skeletal muscle tissue development
GO:0045880 ISS:UniProtKB P positive regulation of smoothened signaling pathway
GO:0051155 IMP:MGI P positive regulation of striated muscle cell differentiation
GO:0045944 IDA:BHF-UCL P positive regulation of transcription from RNA polymerase II promoter
GO:0045893 IDA:UniProtKB P positive regulation of transcription, DNA-templated
GO:2000063 IMP:UniProtKB P positive regulation of ureter smooth muscle cell differentiation
GO:0045059 IMP:BHF-UCL P positive thymic T cell selection
GO:0060516 IDA:MGI P primary prostatic bud elongation
GO:0060523 IDA:MGI P prostate epithelial cord elongation
GO:0030850 IMP:MGI P prostate gland development
GO:0034504 IDA:MGI P protein localization to nucleus
GO:0006508 IEA:UniProtKB-KW P proteolysis
GO:0042127 IDA:MGI P regulation of cell proliferation
GO:0060768 IMP:MGI P regulation of epithelial cell proliferation involved in prostate gland development
GO:0010468 IDA:MGI P regulation of gene expression
GO:0060782 IMP:MGI P regulation of mesenchymal cell proliferation involved in prostate gland development
GO:0042481 IMP:BHF-UCL P regulation of odontogenesis
GO:0060685 IDA:MGI P regulation of prostatic bud formation
GO:0030162 IDA:MGI P regulation of proteolysis
GO:0006355 IMP:MGI P regulation of transcription, DNA-templated
GO:0030323 IMP:MGI P respiratory tube development
GO:0060458 IMP:MGI P right lung development
GO:0060662 IDA:MGI P salivary gland cavitation
GO:0007165 TAS:MGI P signal transduction
GO:0043588 IMP:MGI P skin development
GO:0007224 IDA:MGI P smoothened signaling pathway
GO:0021938 IDA:MGI P smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation
GO:0061053 IMP:BHF-UCL P somite development
GO:0021513 IMP:MGI P spinal cord dorsal/ventral patterning
GO:0021522 IGI:MGI P spinal cord motor neuron differentiation
GO:0048864 IMP:BHF-UCL P stem cell development
GO:0051146 IDA:MGI P striated muscle cell differentiation
GO:0014706 IMP:MGI P striated muscle tissue development
GO:0021978 IMP:MGI P telencephalon regionalization
GO:0021794 IMP:MGI P thalamus development
GO:0048538 IMP:BHF-UCL P thymus development
GO:0030878 IMP:MGI P thyroid gland development
GO:0060438 IMP:MGI P trachea development
GO:0060439 IMP:MGI P trachea morphogenesis
GO:0001944 IMP:MGI P vasculature development
GO:0001570 IDA:MGI P vasculogenesis
GO:0060979 TAS:DFLAT P vasculogenesis involved in coronary vascular morphogenesis

KEGG Pathway Links

KEGG Pathway ID Description
mmu05217 Basal cell carcinoma

Domain Information

InterPro Annotations

Accession Description
IPR001657 Hedgehog protein
IPR001767 Hedgehog protein, Hint domain
IPR009045 Hedgehog signalling/DD-peptidase zinc-binding domain
IPR000320 Hedgehog, N-terminal signalling domain
IPR028992 Hedgehog/Intein (Hint) domain
IPR003586 Hint domain C-terminal
IPR003587 Hint domain N-terminal
IPR006141 Intein splice site

UniProt Annotations

Entry Information

Gene Name
sonic hedgehog
Protein Entry
SHH_MOUSE
UniProt ID
Q62226
Species
Mouse

Comments

Comment Type Description
Developmental Stage First detectable during gastrulation.
Domain The sonic hedgehog protein N-product binds calcium and zinc ions; this stabilizes the protein fold and is essential for protein-protein interactions mediated by this domain. {ECO:0000269|PubMed:18794898, ECO:0000269|PubMed:19561611, ECO:0000269|PubMed:20519495}.
Function Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior- posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO. {ECO:0000269|PubMed:14687547, ECO:0000269|PubMed:7736596}.
Induction By retinoic acid.
Miscellaneous Mice overexpressing Shh display digit duplications in both forelimbs and hindlimbs.
Ptm Cholesterylation is required for N-product targeting to lipid rafts and multimerization. {ECO:0000269|PubMed:8824192}.
Ptm N-palmitoylation of Cys-25 by HHAT is required for N-product multimerization and full activity. {ECO:0000269|PubMed:11486055, ECO:0000269|PubMed:15075292}.
Ptm The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N- terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity. {ECO:0000269|PubMed:7720571, ECO:0000269|PubMed:7736596, ECO:0000269|PubMed:7891723}.
Similarity Belongs to the hedgehog family. {ECO:0000305}.
Subcellular Location Sonic hedgehog protein C-product: Secreted, extracellular space. Note=The C-terminal peptide diffuses from the cell.
Subcellular Location Sonic hedgehog protein N-product: Cell membrane; Lipid-anchor. Note=The N-product either remains associated with lipid rafts at the cell surface, or forms freely diffusible active multimers with its hydrophobic lipid-modified N- and C-termini buried inside.
Subunit Interacts with HHATL/GUP1 which negatively regulates HHAT-mediated palmitoylation of the SHH N-terminus. N-product is active as a multimer. Interacts with BOC and CDON. Interacts with HHIP. {ECO:0000269|PubMed:15075292, ECO:0000269|PubMed:18081866, ECO:0000269|PubMed:18794898, ECO:0000269|PubMed:19561611, ECO:0000269|PubMed:20519495, ECO:0000269|PubMed:7477329}.
Tissue Specificity Expressed in a number of embryonic tissues including the notochord, ventral neural tube, floor plate, lung bud, zone of polarizing activity and posterior distal mesenchyme of limbs. In the adult, expressed in lung and neural retina.

Identical and Related Proteins

Unique RefSeq proteins for LMP002282 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
21617861 RefSeq NP_033196 437 sonic hedgehog protein precursor

Identical Sequences to LMP002282 proteins

Reference Database Accession Length Protein Name
GI:21617861 GenBank ABT07688.1 437 Sequence 20 from patent US 7220838
GI:21617861 GenBank ADC14675.1 437 Sequence 16 from patent US 7632812
GI:21617861 GenBank ADR90187.1 437 Sequence 20 from patent US 7741451
GI:21617861 GenBank AED17249.1 437 Sequence 564 from patent US 7879544
GI:21617861 GenBank AEW39336.1 437 Sequence 20 from patent US 8071733
GI:21617861 GenBank AGF05662.1 437 Sequence 20 from patent US 8362216

Related Sequences to LMP002282 proteins

Reference Database Accession Length Protein Name
GI:21617861 EMBL CAD10615.1 437 unnamed protein product, partial [Mus sp.]
GI:21617861 EMBL CAM31800.1 437 unnamed protein product, partial [Mus musculus]
GI:21617861 GenBank AAE56535.1 437 Sequence 11 from patent US 6165747
GI:21617861 GenBank AAE78685.1 437 Sequence 11 from patent US 6271363
GI:21617861 GenBank AAQ40732.1 437 Sequence 11 from patent US 6576237
GI:21617861 GenBank ACN02117.1 437 Sequence 13 from patent US 7479539