Gene/Proteome Database (LMPD)

LMPD ID
LMP002290
Gene ID
228026
Species
Mus musculus (Mouse)
Gene Name
pyruvate dehydrogenase kinase, isoenzyme 1
Gene Symbol
Pdk1
Synonyms
B830012B01; D530020C15Rik
Alternate Names
pyruvate dehydrogenase kinase, isozyme 1; PDH kinase 1; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
Chromosome
2
Map Location
2 C3|2
EC Number
2.7.11.2

Proteins

pyruvate dehydrogenase kinase, isozyme 1 precursor
Refseq ID NP_766253
Protein GI 110625975
UniProt ID Q8BFP9
mRNA ID NM_172665
Length 434
RefSeq Status VALIDATED
MRLARLLRGGTSVRPLCAVPCASRSLASASASGSGPASELGVPGQVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWYIQSLQELLDFKDKSAEDAKTIYEFTDTVIRIRNRHNDVIPTMAQGVTEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGSPSHRKHIGSINPNCDVVEVIKDGYENARRLCDLYYVNSPELELEELNAKSPGQTIQVVYVPSHLYHMVFELFKNAMRATMEHHADKGVYPPIQVHVTLGEEDLTVKMSDRGGGVPLRKIDRLFNYMYSTAPRPRVETSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKALSTESVERLPVYNKAAWKHYKANHEADDWCVPSREPKDMTTFRSS
transit_peptide: 1..26 inference: non-experimental evidence, no additional details recorded note: Mitochondrion (Potential); propagated from UniProtKB/Swiss-Prot (Q8BFP9.2) calculated_mol_wt: 2784 peptide sequence: MRLARLLRGGTSVRPLCAVPCASRSL mat_peptide: 27..434 product: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (Q8BFP9.2) calculated_mol_wt: 46229 peptide sequence: ASASASGSGPASELGVPGQVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWYIQSLQELLDFKDKSAEDAKTIYEFTDTVIRIRNRHNDVIPTMAQGVTEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGSPSHRKHIGSINPNCDVVEVIKDGYENARRLCDLYYVNSPELELEELNAKSPGQTIQVVYVPSHLYHMVFELFKNAMRATMEHHADKGVYPPIQVHVTLGEEDLTVKMSDRGGGVPLRKIDRLFNYMYSTAPRPRVETSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKALSTESVERLPVYNKAAWKHYKANHEADDWCVPSREPKDMTTFRSS

Gene Information

Entrez Gene ID
228026
Gene Name
pyruvate dehydrogenase kinase, isoenzyme 1
Gene Symbol
Pdk1
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005967 IDA:UniProtKB C mitochondrial pyruvate dehydrogenase complex
GO:0005739 IDA:MGI C mitochondrion
GO:0005886 ISA:MGI C plasma membrane
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0004674 IEA:UniProtKB-KW F protein serine/threonine kinase activity
GO:0004740 ISS:UniProtKB F pyruvate dehydrogenase (acetyl-transferring) kinase activity
GO:0008283 ISS:UniProtKB P cell proliferation
GO:0006006 IEA:UniProtKB-KW P glucose metabolic process
GO:0097411 ISS:UniProtKB P hypoxia-inducible factor-1alpha signaling pathway
GO:0035556 ISA:MGI P intracellular signal transduction
GO:0008631 ISS:UniProtKB P intrinsic apoptotic signaling pathway in response to oxidative stress
GO:0006468 ISS:GOC P protein phosphorylation
GO:0010510 IMP:UniProtKB P regulation of acetyl-CoA biosynthetic process from pyruvate
GO:0010906 ISS:UniProtKB P regulation of glucose metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
mmu04066 HIF-1 signaling pathway

REACTOME Pathway Links

REACTOME Pathway ID Description
5893557 Regulation of pyruvate dehydrogenase (PDH) complex

Domain Information

InterPro Annotations

Accession Description
IPR018955 Branched-chain alpha-ketoacid dehydrogenase kinase/Pyruvate dehydrogenase kinase, N-terminal
IPR003594 Histidine kinase-like ATPase, C-terminal domain
IPR005467 Signal transduction histidine kinase, core

UniProt Annotations

Entry Information

Gene Name
pyruvate dehydrogenase kinase, isoenzyme 1
Protein Entry
PDK1_MOUSE
UniProt ID
Q8BFP9
Species
Mouse

Comments

Comment Type Description
Catalytic Activity ATP + [pyruvate dehydrogenase (acetyl- transferring)] = ADP + [pyruvate dehydrogenase (acetyl- transferring)] phosphate.
Function Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress (By similarity). {ECO:0000250}.
Induction Up-regulated by glucose and palmitate. Up- regulated via the HIF1A signaling pathway in response to hypoxia. {ECO:0000269|PubMed:16517406, ECO:0000269|PubMed:16631612}.
Ptm Phosphorylated by constitutively activated ABL1, FGFR1, FLT3 and JAK2 (in vitro), and this may also occur in cancer cells that express constitutively activated ABL1, FGFR1, FLT3 and JAK2. Phosphorylation at Tyr-241 and Tyr-242 strongly increases kinase activity, while phosphorylation at Tyr-136 has a lesser effect (By similarity). {ECO:0000250}.
Similarity Belongs to the PDK/BCKDK protein kinase family. {ECO:0000305}.
Similarity Contains 1 histidine kinase domain. {ECO:0000255|PROSITE-ProRule:PRU00107}.
Subcellular Location Mitochondrion matrix {ECO:0000250}.
Subunit Homodimer, and heterodimer with PDK2. Interacts with the pyruvate dehydrogenase complex subunit DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3) (By similarity). {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP002290 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
110625975 RefSeq NP_766253 434 pyruvate dehydrogenase kinase, isozyme 1 precursor

Identical Sequences to LMP002290 proteins

Reference Database Accession Length Protein Name
GI:110625975 DBBJ BAE32134.1 434 unnamed protein product [Mus musculus]
GI:110625975 GenBank EDL27100.1 434 pyruvate dehydrogenase kinase, isoenzyme 1, isoform CRA_b [Mus musculus]
GI:110625975 GenBank EDL27102.1 434 pyruvate dehydrogenase kinase, isoenzyme 1, isoform CRA_d [Mus musculus]
GI:110625975 GenBank EDL27104.1 434 pyruvate dehydrogenase kinase, isoenzyme 1, isoform CRA_f [Mus musculus]
GI:110625975 SwissProt Q8BFP9.2 434 RecName: Full=[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; AltName: Full=Pyruvate dehydrogenase kinase isoform 1; Short=PDH kinase 1; Flags: Precursor [Mus musculus]

Related Sequences to LMP002290 proteins

Reference Database Accession Length Protein Name
GI:110625975 DBBJ BAC32879.1 434 unnamed protein product [Mus musculus]
GI:110625975 GenBank AAA62851.1 434 pyruvate dehydrogenase kinase kinase [Rattus norvegicus]
GI:110625975 GenBank AAH27196.1 432 Pdk1 protein [Mus musculus]
GI:110625975 GenBank AAH89783.1 434 Pyruvate dehydrogenase kinase, isozyme 1 [Rattus norvegicus]
GI:110625975 RefSeq NP_446278.2 434 pyruvate dehydrogenase kinase, isozyme 1 precursor [Rattus norvegicus]
GI:110625975 SwissProt Q63065.1 434 RecName: Full=[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; AltName: Full=PDK p48; AltName: Full=Pyruvate dehydrogenase kinase isoform 1; Short=PDH kinase 1; Flags: Precursor [Rattus norvegicus]