Gene/Proteome Database (LMPD)

LMPD ID
LMP003961
Gene ID
216439
Species
Mus musculus (Mouse)
Gene Name
ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
Gene Symbol
Agap2
Synonyms
AGAP-2; Centg1; PIKE; cnt-g1; mKIAA0167
Chromosome
10
Map Location
10 D3|10
Summary
The protein encoded by this gene is a member of the centaurin GTPase family. This gene product regulates the activity of multiple kinases, including PI3K. Reduced expression of this gene results in multiple defects, including neural deficiencies, while increased expression of this gene has been observed in some tumors. Alternative splicing results in multiple protein isoforms. [provided by RefSeq, Jul 2014]
Orthologs
View orthologs and multiple alignments for Agap2

Proteins

arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform 1
Refseq ID NP_001028435
Protein GI 84370300
UniProt ID Q3UHD9
mRNA ID NM_001033263
Length 1186
RefSeq Status REVIEWED
MSRGAGALQRRTTTYLISLTLVKLESVPPPPPSPSAAAAGAPGARGSEPRDPGSPRGSEEPGKKRHERLFHRQDALWISTSSAGTGGAEPPALSPAPASPARPVSPAPGRRLSLWAAPPGPPLSGGLSPDSKPGGAPSSSRRPLLSSPSWGGPEPEGRTGGGVPGSSSPHPGTGSRRLKVAPPPPAPKPFKTVTTSGAKAGGGKGAGSRLSWPESEGKPRVKGSKSTAGTGASAAAAGGGGSAAVTTSGGVGAGAGTRGKLSPRKGKSKTLDNSDLHPGPSAGSPPLTVPAIPVPATSVTATSTQPLGPAPPITLEPPAPGLKRGREGGRASTRDRKMLKFISGIFTKSTGGPPGPGPLPGPQGLSSSSGSRELLGAELRTSPKAVVNSQEWTLSRSIPELRLGVLGDVRSGKSSLIHRFLTGSYQVLEKIESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVIFVFSLEDESSFQAVSRLHGQLSSLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCTDMKRCSYYETCATYGLNVDRVFQEVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSPNVGHRELRAEAAAVAGLSTPGSLHRAAKRRTSLFANRRGSDAEKRSLDSRGETTGSGRAIPIKQSFLLKRSGNSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGLVKDMSTVQMGEGPEASTPMPSPSPSPSSLQLPTDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLSTPSKTEGSAVQAEAKRKMWKLKSFGSLRNIYKAEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCCESSKVKLRTDSQSEAVAIQAIRNAKGNSTCVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPTRDSSREERESWIRAKYEQLLFLAPLGTTEEPLGRQLWAAVEAQDVAAVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCADILLQHGCPGEGGSTATTPSAATTPSITATPSPRRRSSAASLGRVDTTIALV
arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform 2
Refseq ID NP_001287943
Protein GI 666638002
UniProt ID Q3UHD9
mRNA ID NM_001301014
Length 1166
RefSeq Status REVIEWED
MSRGAGALQRRTTTYLISLTLVKLESVPPPPPSPSAAAAGAPGARGSEPRDPGSPRGSEEPGKKRHERLFHRQDALWISTSSAGTGGAEPPALSPAPASPARPVSPAPGRRLSLWAAPPGPPLSGGLSPDSKPGGAPSSSRRPLLSSPSWGGPEPEGRTGGGVPGSSSPHPGTGSRRLKVAPPPPAPKPFKTVTTSGAKAGGGKGAGSRLSWPESEGKPRVKGSKSTAGTGASAAAAGGGGSAAVTTSGGVGAGAGTRGKLSPRKGKSKTLDNSDLHPGPSAGSPPLTVPAIPVPATSVTATSTQPLGPAPPITLEPPAPGLKRGREGGRASTRDRKMLKFISGIFTKSTGGPPGPGPLPGPQGLSSSSGSRELLGAELRTSPKAVVNSQEWTLSRSIPELRLGVLGDVRSGKSSLIHRFLTGSYQVLEKIESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVIFVFSLEDESSFQAVSRLHGQLSSLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCTDMKRCSYYETCATYGLNVDRVFQEVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSPNVGHRELRAEAAAVAGLSTPGSLHRAAKRRTSLFANRRGSDAEKRSLDSRGETTGSGRAIPIKQSFLLKRSGNSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGLVKDMSTVQMGEGPEASTPMPSPSPSPSSLQLPTDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLSTPSKTEGSAVQAEEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCCESSKVKLRTDSQSEAVAIQAIRNAKGNSTCVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPTRDSSREERESWIRAKYEQLLFLAPLGTTEEPLGRQLWAAVEAQDVAAVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCADILLQHGCPGEGGSTATTPSAATTPSITATPSPRRRSSAASLGRVDTTIALV

Gene Information

Entrez Gene ID
216439
Gene Name
ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
Gene Symbol
Agap2
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005739 IEA:Ensembl C mitochondrion
GO:0005730 IEA:Ensembl C nucleolus
GO:0008060 IEA:InterPro F ARF GTPase activator activity
GO:0005525 IEA:UniProtKB-KW F GTP binding
GO:0030295 IEA:Ensembl F protein kinase activator activity
GO:0008270 IEA:InterPro F zinc ion binding
GO:0060749 IMP:MGI P mammary gland alveolus development
GO:0043066 IMP:MGI P negative regulation of apoptotic process
GO:2001240 IEA:Ensembl P negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
GO:0046427 IMP:MGI P positive regulation of JAK-STAT cascade
GO:0033601 IMP:MGI P positive regulation of mammary gland epithelial cell proliferation
GO:0045944 IMP:MGI P positive regulation of transcription from RNA polymerase II promoter
GO:0032312 IEA:InterPro P regulation of ARF GTPase activity
GO:0007264 IEA:InterPro P small GTPase mediated signal transduction

KEGG Pathway Links

KEGG Pathway ID Description
ko04144 Endocytosis
mmu04144 Endocytosis
mmu04068 FoxO signaling pathway

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY3DJ-1 cyclic AMP biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
5893042 Axon guidance
5893043 Developmental Biology
5893637 Netrin-1 signaling

Domain Information

InterPro Annotations

Accession Description
IPR002110 Ankyrin repeat
IPR020683 Ankyrin repeat-containing domain
IPR001164 Arf GTPase activating protein
IPR013684 Mitochondrial Rho-like
IPR027417 P-loop containing nucleoside triphosphate hydrolase
IPR001849 Pleckstrin homology domain
IPR011993 Pleckstrin homology-like domain

UniProt Annotations

Entry Information

Gene Name
ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
Protein Entry
Q3UHD9_MOUSE
UniProt ID
Q3UHD9
Species
Mouse

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q3UHD9-1; Sequence=Displayed; Name=2; IsoId=Q3UHD9-2; Sequence=VSP_018542; Note=No experimental confirmation available.;
Domain Arf-GAP domain interacts with G domain and may regulate its GTPase activity.
Domain G domain binds GTP and has GTPase activity.
Domain PH domain binds phospholipids and is required for nuclear targeting.
Enzyme Regulation GAP activity is stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and, to a lesser extent, by phosphatidylinositol 3,4,5-trisphosphate (PIP3). Phosphatidic acid potentiates PIP2 stimulation (By similarity). {ECO:0000250}.
Function GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. Aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase (By similarity). {ECO:0000250}.
Similarity Belongs to the centaurin gamma-like family. {ECO:0000305}.
Similarity Contains 1 Arf-GAP domain. {ECO:0000255|PROSITE- ProRule:PRU00288}.
Similarity Contains 1 PH domain. {ECO:0000255|PROSITE- ProRule:PRU00145}.
Similarity Contains 2 ANK repeats. {ECO:0000255|PROSITE- ProRule:PRU00023}.
Subcellular Location Cytoplasm. Nucleus {ECO:0000250}.
Subunit Interacts with EPB41L1, PLCG1, NF2, HOMER1 and HOMER2. {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP003961 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
84370300 RefSeq NP_001028435 1186 arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform 1
666638002 RefSeq NP_001287943 1166 arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform 2

Identical Sequences to LMP003961 proteins

Reference Database Accession Length Protein Name
GI:84370300 DBBJ BAE27918.1 1186 unnamed protein product [Mus musculus]
GI:84370300 GenBank AAI56809.1 1186 ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 [synthetic construct]
GI:84370300 SwissProt Q3UHD9.1 1186 RecName: Full=Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2; Short=AGAP-2; AltName: Full=Centaurin-gamma-1; Short=Cnt-g1; AltName: Full=Phosphatidylinositol 3-kinase enhancer; Short=PIKE [Mus musculus]

Related Sequences to LMP003961 proteins

Reference Database Accession Length Protein Name
GI:666638002 DBBJ BAE27918.1 1186 unnamed protein product [Mus musculus]
GI:84370300 GenBank AAM97539.1 1186 PI 3-kinase enhancer long isoform, partial [Rattus norvegicus]
GI:84370300 GenBank EDM16498.1 1186 centaurin, gamma 1, partial [Rattus norvegicus]
GI:666638002 GenBank EDM16498.1 1186 centaurin, gamma 1, partial [Rattus norvegicus]
GI:666638002 GenBank AAI56809.1 1186 ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 [synthetic construct]
GI:666638002 RefSeq NP_001028435.1 1186 arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform 1 [Mus musculus]
GI:84370300 RefSeq NP_075415.2 1186 arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 [Rattus norvegicus]
GI:84370300 RefSeq XP_005358014.1 1186 PREDICTED: arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X1 [Microtus ochrogaster]
GI:666638002 RefSeq XP_006241561.1 1166 PREDICTED: arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X1 [Rattus norvegicus]
GI:84370300 RefSeq NP_001287943.1 1166 arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform 2 [Mus musculus]
GI:84370300 SwissProt Q8CGU4.1 1186 RecName: Full=Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2; Short=AGAP-2; AltName: Full=Centaurin-gamma-1; Short=Cnt-g1; AltName: Full=Phosphatidylinositol 3-kinase enhancer; Short=PIKE [Rattus norvegicus]
GI:666638002 SwissProt Q3UHD9.1 1186 RecName: Full=Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2; Short=AGAP-2; AltName: Full=Centaurin-gamma-1; Short=Cnt-g1; AltName: Full=Phosphatidylinositol 3-kinase enhancer; Short=PIKE [Mus musculus]