Gene/Proteome Database (LMPD)

LMPD ID
LMP006338
Gene ID
210992
Species
Mus musculus (Mouse)
Gene Name
lysophosphatidylcholine acyltransferase 1
Gene Symbol
Lpcat1
Synonyms
2900035H07Rik; Aytl2; BB137372; BC005662; C87117; LPCAT; rd11
Alternate Names
lysophosphatidylcholine acyltransferase 1; LPCAT-1; mLPCAT1; lysoPAFAT; LPC acyltransferase 1; acyltransferase like 2; acyltransferase-like 2; lysoPC acyltransferase 1; lyso-PAF acetyltransferase; acetyl-CoA:lyso-PAF acetyltransferase; 1-acylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase; acetyl-CoA:lyso-platelet-activating factor acetyltransferase
Chromosome
13
Map Location
13 C1|13
EC Number
2.3.1.23

Proteins

lysophosphatidylcholine acyltransferase 1
Refseq ID NP_663351
Protein GI 148747363
UniProt ID Q3TFD2
mRNA ID NM_145376
Length 534
RefSeq Status PROVISIONAL
MRLRGRGPRAAPSSSSGAGDARRLAPPGRNPFVHELRLSALQKAQVAFMTLTLFPIRLLFAAFMMLLAWPFALLASLGPPDKEPEQPLALWRKVVDFLLKAIMRTMWFAGGFHRVAVKGRQALPTEAAILTLAPHSSYFDAIPVTMTMSSIVMKAESRDIPIWGTLIRYIRPVFVSRSDQDSRRKTVEEIKRRAQSNGKWPQIMIFPEGTCTNRTCLITFKPGAFIPGVPVQPVVLRYPNKLDTITWTWQGPGALKILWLTLCQFQNQVEIEFLPVYCPSEEEKRNPALYASNVRRVMAKALGVSVTDYTFEDCQLALAEGQLRLPADTCLLEFARLVRGLGLKPENLEKDLDKYSESARMKRGEKIRLPEFAAYLEVPVSDALEDMFSLFDESGGGEIDLREYVVALSVVCRPSQTLATIQLAFKMYGSPEDGSIDEANLSCILKTALGVSELTVTDLFQAIDQEDKGRITFDDFCGFAEMYPDYAEDYLYPDQTHFDSCAQTPPAPTPNGFCIDFSPENSDFGRKNSCKKAD

Gene Information

Entrez Gene ID
210992
Gene Name
lysophosphatidylcholine acyltransferase 1
Gene Symbol
Lpcat1
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IDA:UniProtKB C endoplasmic reticulum
GO:0005794 IDA:UniProtKB C Golgi apparatus
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0005811 IEA:UniProtKB-KW C lipid particle
GO:0047184 IDA:UniProtKB F 1-acylglycerophosphocholine O-acyltransferase activity
GO:0047159 IDA:UniProtKB F 1-alkenylglycerophosphocholine O-acyltransferase activity
GO:0047192 IEA:UniProtKB-EC F 1-alkylglycerophosphocholine O-acetyltransferase activity
GO:0047191 IDA:UniProtKB F 1-alkylglycerophosphocholine O-acyltransferase activity
GO:0005509 IEA:InterPro F calcium ion binding
GO:2001246 IDA:MGI P negative regulation of phosphatidylcholine biosynthetic process
GO:0036151 IEA:Ensembl P phosphatidylcholine acyl-chain remodeling
GO:0008654 IDA:UniProtKB P phospholipid biosynthetic process
GO:0045732 IDA:MGI P positive regulation of protein catabolic process
GO:0060041 IMP:MGI P retina development in camera-type eye
GO:0043129 IDA:MGI P surfactant homeostasis

KEGG Pathway Links

KEGG Pathway ID Description
mmu00565 Ether lipid metabolism
mmu00564 Glycerophospholipid metabolism

REACTOME Pathway Links

REACTOME Pathway ID Description
5893974 Acyl chain remodelling of PC
5893975 Acyl chain remodelling of PG

Domain Information

InterPro Annotations

Accession Description
IPR018247 EF-Hand 1, calcium-binding site
IPR002048 EF-hand domain
IPR011992 EF-hand domain pair
IPR002123 Phospholipid/glycerol acyltransferase

UniProt Annotations

Entry Information

Gene Name
lysophosphatidylcholine acyltransferase 1
Protein Entry
PCAT1_MOUSE
UniProt ID
Q3TFD2
Species
Mouse

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q3TFD2-1; Sequence=Displayed; Name=2; IsoId=Q3TFD2-2; Sequence=VSP_019914; Note=No experimental confirmation available.; Name=3; IsoId=Q3TFD2-3; Sequence=VSP_019913; Note=No experimental confirmation available.;
Biophysicochemical Properties Kinetic parameters: KM=2.3 uM for 1-palmitoyl-LPC {ECO:0000269|PubMed:16704971, ECO:0000269|PubMed:18285344}; KM=3 uM for palmitoyl-CoA {ECO:0000269|PubMed:16704971, ECO:0000269|PubMed:18285344}; pH dependence: Optimum pH is 7.5. {ECO:0000269|PubMed:16704971, ECO:0000269|PubMed:18285344};
Catalytic Activity Acetyl-CoA + 1-alkyl-sn-glycero-3- phosphocholine = CoA + 2-acetyl-1-alkyl-sn-glycero-3- phosphocholine. {ECO:0000269|PubMed:16704971}.
Catalytic Activity Acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine = CoA + 1,2-diacyl-sn-glycero-3-phosphocholine. {ECO:0000269|PubMed:16704971}.
Developmental Stage Expression increases steadily throughout embryogenesis and decreases slightly in the adult. {ECO:0000269|PubMed:16864775}.
Domain The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins. {ECO:0000250}.
Domain The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphocholine. {ECO:0000250}.
Enzyme Regulation Not activated by inflammatory stimulation. Inhibited by Cu(2+) and Fe(2+). Activity is not affected by Co(2+), Mg(2+) or Mn(2+). {ECO:0000269|PubMed:18285344}.
Function Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-independent. Mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC). Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively. May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology. {ECO:0000269|PubMed:16704971, ECO:0000269|PubMed:18156367, ECO:0000269|PubMed:18285344}.
Induction Constitutively expressed. Not induced by inflammatory stimulation. {ECO:0000269|PubMed:18285344}.
Pathway Lipid metabolism; phospholipid metabolism.
Sequence Caution Sequence=AAH05662.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=BAC32594.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=BAC32760.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
Similarity Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. {ECO:0000305}.
Similarity Contains 2 EF-hand domains. {ECO:0000255|PROSITE- ProRule:PRU00448}.
Subcellular Location Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein. Lipid droplet {ECO:0000250}. Note=May adopt a monotopic topology when embedded in the lipid monolayer of the lipid droplet, with both termini exposed to the cytoplasm. {ECO:0000250}.
Tissue Specificity Predominantly expressed in lung where it is enriched in alveolar type II cells. Expressed at lower levels in spleen and brain. Also detected in erythroleukemic cells and reticulocytes. Weakly or not expressed in other tissues. {ECO:0000269|PubMed:16704971, ECO:0000269|PubMed:16864775, ECO:0000269|PubMed:18156367}.

Identical and Related Proteins

Unique RefSeq proteins for LMP006338 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
148747363 RefSeq NP_663351 534 lysophosphatidylcholine acyltransferase 1

Identical Sequences to LMP006338 proteins

Reference Database Accession Length Protein Name
GI:148747363 DBBJ BAE40966.1 534 unnamed protein product [Mus musculus]
GI:148747363 DBBJ BAE33166.1 534 unnamed protein product [Mus musculus]
GI:148747363 DBBJ BAE41980.1 534 unnamed protein product [Mus musculus]
GI:148747363 DBBJ BAE94687.2 534 LPC acyltransferase [Mus musculus]
GI:148747363 GenBank EDL37050.1 534 acyltransferase like 2, isoform CRA_a [Mus musculus]
GI:148747363 SwissProt Q3TFD2.1 534 RecName: Full=Lysophosphatidylcholine acyltransferase 1; Short=LPC acyltransferase 1; Short=LPCAT-1; Short=LysoPC acyltransferase 1; Short=mLPCAT1; AltName: Full=1-acylglycerophosphocholine O-acyltransferase; AltName: Full=1-alkylglycerophosphocholine O-acetyltransferase; AltName: Full=Acetyl-CoA:lyso-platelet-activating factor acetyltransferase; Short=Acetyl-CoA:lyso-PAF acetyltransferase; Short=Lyso-PAF acetyltransferase; Short=LysoPAFAT; AltName: Full=Acyltransferase-like 2 [Mus musculus]

Related Sequences to LMP006338 proteins

Reference Database Accession Length Protein Name
GI:148747363 DBBJ BAC38353.1 534 unnamed protein product [Mus musculus]
GI:148747363 DBBJ BAE42540.1 534 unnamed protein product [Mus musculus]
GI:148747363 GenBank EDL87651.1 534 acyltransferase like 2 (predicted), isoform CRA_a [Rattus norvegicus]
GI:148747363 RefSeq NP_001094205.1 534 lysophosphatidylcholine acyltransferase 1 [Rattus norvegicus]
GI:148747363 RefSeq XP_005356766.1 534 PREDICTED: lysophosphatidylcholine acyltransferase 1 [Microtus ochrogaster]
GI:148747363 SwissProt Q1HAQ0.2 534 RecName: Full=Lysophosphatidylcholine acyltransferase 1; Short=LPC acyltransferase 1; Short=LPCAT-1; Short=LysoPC acyltransferase 1; AltName: Full=1-acylglycerophosphocholine O-acyltransferase; AltName: Full=1-alkylglycerophosphocholine O-acetyltransferase; AltName: Full=Acetyl-CoA:lyso-platelet-activating factor acetyltransferase; Short=Acetyl-CoA:lyso-PAF acetyltransferase; Short=Lyso-PAF acetyltransferase; Short=LysoPAFAT; AltName: Full=Acyltransferase-like 2 [Rattus norvegicus]