Gene/Proteome Database (LMPD)
Proteins
cubilin precursor | |
---|---|
Refseq ID | NP_001074553 |
Protein GI | 124487348 |
UniProt ID | Q9JLB4 |
mRNA ID | NM_001081084 |
Length | 3623 |
RefSeq Status | VALIDATED |
MASHFLWGFVTLLMVPGLDGETGTPEQKLQKRIADLHQPRMTTEEGNLVFLTSSAQNIEFRTGSLGKIKLNDDDLGECLHQIQRNKDDIIDLKRNTTGLPQNILSQVHQLNSKLVDLERDFQSLQQNVERKVCSSNPCHNGGTCVNLHDSFICICPSQWKGLFCSEDVNECVLYAGTPFGCQSGSTCVNTMGSFRCDCTPDTYGPQCASKYNDCEQGSQQLCKHGICEDLQRVYHGQLRFNCICDAGWTTLPNGISCTEDKDECSLQPSPCSEHAQCFNTQGSFYCGACPKGWQGNGYQCQDINECEINNGGCSQAPLVPCLNTPGSFTCGNCPAGFSGDGRVCTPLDICSIHNGGCHPDATCSSSSVLGSLLPVCTCPPGYTGNGYGSNGCVRLSNMCSRHPCVNGQCIETVSSYFCKCDSGWFGQNCTENINECVSNPCLNGGTCIDGVNGFTCDCTSSWTGYYCQTPQAACGGILSGTQGTFAYQSPNDTYVHNVNCFWVVRTDEEKVLHITFTFFDLESASNCPREYLQIHDGDSSADFPLGRYCGSTPPQGVHSSANSLYFHLYSEYIKRGRGFTARWEAKLPECGGILTGNYGSITSPGYPGNYPPGRDCVWNLLVSPGSLITFTFGTLSLESHNDCSKDYLEIRDGPFHHDPILGKFCTSLSTPPLQTTGPAARIHFHSDSETSDKGFHITYLTTPSDLYCGGNYTDTEGELLLPPLTGPFSHSRQCVYLISQPQGEQIVINFTHVELESQRGCSHTFIEVGDHESLLRKICGNETLFPIRSISNNVWIRLRIDALVQKASFRADYQVACGGELRGEGVIRSPFYPNAYAGRRTCRWTISQPPREVVLLNFTDFQIGSSSSCDTDYIEIGPSSVLGSPGNEKFCGTNIPSFITSVYNVLYVTFVKSSSMENRGFMAMFSSEKLECGKVLTESTGIIESPGHPNVYPSGVNCTWHIVVQRGQLIRLVFSSFYLEFHYNCANDYLEVYDTIAQTSLGRYCGKSIPPSLTSSSHSIKLIFVSDSALAHEGFSINYEAINASSVCLYDYTDNFGRLSSPNFPNNYPHNWNCVYRITVGLNQQIALHFTDFALEDYFGPKCVDFVEIRDGGFETSPLIGIYCGSVFPPRIISHSNKLWLRFKSDTALTARGFSAYWDASSTGCGGNLTTPTGVLTSPNYPMPYYHSSECYWRLEASRGSPFLLEFQDFHLEHHPNCSLDYLAVFDGPSTNSRLINKLCGDTPPAPIRSSKDIVLLKLRTDAGQQGRGFEINYRQTCDNVVIVNKTSGILESINYPNPYDKDQRCNWTIQATTGNTVNYTFLEFDVENYVNCSTDYLELYDGPQRIGRYCGENIPPPGATTGSKLIVVFHTDGVDSGEKGFKMHWFIHGCGGEMSGTMGSFSSPGYPNSYPHNKECIWNIRVAPGNSIQLTIHDFDVEYHASCKYDTLEIYTGLDFHSPRIAQLCSRSPSANPMQISSTDNELAIRFKTDSSLNGRGFNASWRAVPGGCGGIFQVSRGEIHSPNYPNNYRANTECSWIIQVEKYHRVLLNITDFDLEATDSCLMTYDGSSSANTRVATVCGRQQPPNSITSSGNSLFVRFQSGSSSQSRGFRAQFRQECGAHIITDSSDSISSPLYPANYPNNQNCTWIIEAQPPFNHIALSFTHFHLQSSTDCTRDFVEILDGRDSDAPVQGRYCGTSLPHPIISFGNALTVRFVSDSVYGFDGFHAIYSASTSACGGTFYTGDGIFNSPGYPEDYHSNTECVWNIASSPGNHLQLSFLSFQLENSLNCNKDFVEIREGNATGHLMGRYCGNSLPGNYSSIEGHNLWVRFVSDGSGTGMGFQARFKNIFGNDNIVGTHGKIATPFWPGNYPLNSNYRWTVNVDSSHIIHGRILEMDIELTTNCFYDSLKIYDGFDIHSRLIGTYCGTQRESFSSSRNSLTFQFSSDSSKSGRGFLLEWFAVDVSNVTLPTIAPGACGGYMVTGDTPVFFFSPGWPGPYGNGADCIWIIYAPDSTVELNILSMDIEAQLSCSYDKLIIKDGDSRLSQQLAVLCGRSVPGPIRSTGEYMYIRFTSDGSVTGAGFNASFQKSCGGYLHADRGIITSPKYPDNYLPNLNCSWHVLVQSGLTIAVHFEQPFQIQNRDSSCSQGDYLVLRNGPDNHSPPLGPSGGNGRFCGIYTPSTLFTSDNEMFIQFISDNSNGGQGFKIRYEAKSLACGGTIYIHDANSDGYVTSPNYPANYPQHAECIWILEAPSGRSIQLQFEDQFNIEETPNCSASYLELRDGANSNAPVLSKLCGHTLPRNWVSSRGLMYLKFHTEGGSGYMGFKAKYSIVSCGGTVSGDSGVIESVGYPTRLYANNVFCQWHIQGLPGHYLTIRFEDFNLQSSPGCAKDFVEIWENHTSGILLGRYCGNSIPSSVDTSSNVASIRFVTDGSVTDSGFRLQFKSSREVCGGDLHGPTGTFTSPNYPNPNPHPRICEWTINVHEGRQIILTFTNLRLSTQQSCNTEHLIVFNGIRNNSPRLQKLCSRVNVTNEFKSSGNTMKVIFFTDGSRPYGGFTASYTSSEDAVCGGTLPSVSGGNFSSPGYNGIRDYARNLDCEWTLSNPNRENSSISIHFLGLSLESHQDCTFDVLEFRVGNADGPLIEKFCSLSAPRVPLVIPYPQVWIHFVSNERVEYTGFYVEYSFTNCGGIQTGENGVISSPNYPNLYSRWTQCSWLLEAPEGHTITLTFSDFSVENHPTCTSDSVTVRNGDSPGSPIIGRYCGQSVPGPIQSGSNQLVVTFNTNNQGQSRGFYATWNTNTLGCGGTLHSDNGTIKSPHWPQTFPENSRCSWTAVTHESKHWEISFDSNFRIPSSDSQCRNSFVKVWEGMLETNDALLATSCGNVAPSPIVTLGNIFTAVFQSEEMPAQGFSASFISRCGRTFNSSTGDIVSPNFPKHYDNNMNCNYYIDVAPQSLVILTFVSFHLEDRSAVSGTCDYDGLHIIKGHNLSSTPLVTICGSETLRPLTIDGPVMLNFYSDAYITDFGFKISYRVANCGGIYSGTYGVLNSPSFSYTNYPNNVYCVYSLQVRNDRLILLRFNDFEIVPSNLCSHDYLEVFDGPSIGNRSIGKFCGSTLPQVIKSTNNSLTLLFKTDSSQTARGWKVSFRETIGPQQGCGGYLTEDSKSFVSPDHDSDGLYDKGLNCIWYIIAPENKLVKLTFNAFTLEEPSSPGKCTFDYVQIADGASINSYLGGRFCGSSRPAPFISSGNFLTVQFVSDISIQMRGFNATYTFVDMPCGGTYNATSMPQNTSSPQLSNIRRPFSTCTWVIEAPPHQQVQITVWKLQLPSQDCSRSSLELQDSEQTNGNQVTQFCGANYTTLPVFYSSGSTAVVVFKSDFLNRNSRVHFTYEIADCNREYNQAFGNLKSPGWPQGYANNLDCSIILRAPQNHRISLFFYWFQLEDSRQCMNDFLEVRNGSSSSSPLLGKYCSNLLPNPIFSQSNELYLHFHSDDSDTHHGYEIIWASSPTGCGGTLLGNEGILANPGFPDSYPNNTHCEWTIVAPSGRPLSVGFPFLSIDSPGGCDQNYLILFNGPDANSPPFGPFCGIDTVVAPFHASSNRVFIRFHAEYATVSSGFEIMWSS | |
sig_peptide: 1..20 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 2192 peptide sequence: MASHFLWGFVTLLMVPGLDG mat_peptide: 33..3623 product: Cubilin experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (Q9JLB4.3) calculated_mol_wt: 395529 peptide sequence: IADLHQPRMTTEEGNLVFLTSSAQNIEFRTGSLGKIKLNDDDLGECLHQIQRNKDDIIDLKRNTTGLPQNILSQVHQLNSKLVDLERDFQSLQQNVERKVCSSNPCHNGGTCVNLHDSFICICPSQWKGLFCSEDVNECVLYAGTPFGCQSGSTCVNTMGSFRCDCTPDTYGPQCASKYNDCEQGSQQLCKHGICEDLQRVYHGQLRFNCICDAGWTTLPNGISCTEDKDECSLQPSPCSEHAQCFNTQGSFYCGACPKGWQGNGYQCQDINECEINNGGCSQAPLVPCLNTPGSFTCGNCPAGFSGDGRVCTPLDICSIHNGGCHPDATCSSSSVLGSLLPVCTCPPGYTGNGYGSNGCVRLSNMCSRHPCVNGQCIETVSSYFCKCDSGWFGQNCTENINECVSNPCLNGGTCIDGVNGFTCDCTSSWTGYYCQTPQAACGGILSGTQGTFAYQSPNDTYVHNVNCFWVVRTDEEKVLHITFTFFDLESASNCPREYLQIHDGDSSADFPLGRYCGSTPPQGVHSSANSLYFHLYSEYIKRGRGFTARWEAKLPECGGILTGNYGSITSPGYPGNYPPGRDCVWNLLVSPGSLITFTFGTLSLESHNDCSKDYLEIRDGPFHHDPILGKFCTSLSTPPLQTTGPAARIHFHSDSETSDKGFHITYLTTPSDLYCGGNYTDTEGELLLPPLTGPFSHSRQCVYLISQPQGEQIVINFTHVELESQRGCSHTFIEVGDHESLLRKICGNETLFPIRSISNNVWIRLRIDALVQKASFRADYQVACGGELRGEGVIRSPFYPNAYAGRRTCRWTISQPPREVVLLNFTDFQIGSSSSCDTDYIEIGPSSVLGSPGNEKFCGTNIPSFITSVYNVLYVTFVKSSSMENRGFMAMFSSEKLECGKVLTESTGIIESPGHPNVYPSGVNCTWHIVVQRGQLIRLVFSSFYLEFHYNCANDYLEVYDTIAQTSLGRYCGKSIPPSLTSSSHSIKLIFVSDSALAHEGFSINYEAINASSVCLYDYTDNFGRLSSPNFPNNYPHNWNCVYRITVGLNQQIALHFTDFALEDYFGPKCVDFVEIRDGGFETSPLIGIYCGSVFPPRIISHSNKLWLRFKSDTALTARGFSAYWDASSTGCGGNLTTPTGVLTSPNYPMPYYHSSECYWRLEASRGSPFLLEFQDFHLEHHPNCSLDYLAVFDGPSTNSRLINKLCGDTPPAPIRSSKDIVLLKLRTDAGQQGRGFEINYRQTCDNVVIVNKTSGILESINYPNPYDKDQRCNWTIQATTGNTVNYTFLEFDVENYVNCSTDYLELYDGPQRIGRYCGENIPPPGATTGSKLIVVFHTDGVDSGEKGFKMHWFIHGCGGEMSGTMGSFSSPGYPNSYPHNKECIWNIRVAPGNSIQLTIHDFDVEYHASCKYDTLEIYTGLDFHSPRIAQLCSRSPSANPMQISSTDNELAIRFKTDSSLNGRGFNASWRAVPGGCGGIFQVSRGEIHSPNYPNNYRANTECSWIIQVEKYHRVLLNITDFDLEATDSCLMTYDGSSSANTRVATVCGRQQPPNSITSSGNSLFVRFQSGSSSQSRGFRAQFRQECGAHIITDSSDSISSPLYPANYPNNQNCTWIIEAQPPFNHIALSFTHFHLQSSTDCTRDFVEILDGRDSDAPVQGRYCGTSLPHPIISFGNALTVRFVSDSVYGFDGFHAIYSASTSACGGTFYTGDGIFNSPGYPEDYHSNTECVWNIASSPGNHLQLSFLSFQLENSLNCNKDFVEIREGNATGHLMGRYCGNSLPGNYSSIEGHNLWVRFVSDGSGTGMGFQARFKNIFGNDNIVGTHGKIATPFWPGNYPLNSNYRWTVNVDSSHIIHGRILEMDIELTTNCFYDSLKIYDGFDIHSRLIGTYCGTQRESFSSSRNSLTFQFSSDSSKSGRGFLLEWFAVDVSNVTLPTIAPGACGGYMVTGDTPVFFFSPGWPGPYGNGADCIWIIYAPDSTVELNILSMDIEAQLSCSYDKLIIKDGDSRLSQQLAVLCGRSVPGPIRSTGEYMYIRFTSDGSVTGAGFNASFQKSCGGYLHADRGIITSPKYPDNYLPNLNCSWHVLVQSGLTIAVHFEQPFQIQNRDSSCSQGDYLVLRNGPDNHSPPLGPSGGNGRFCGIYTPSTLFTSDNEMFIQFISDNSNGGQGFKIRYEAKSLACGGTIYIHDANSDGYVTSPNYPANYPQHAECIWILEAPSGRSIQLQFEDQFNIEETPNCSASYLELRDGANSNAPVLSKLCGHTLPRNWVSSRGLMYLKFHTEGGSGYMGFKAKYSIVSCGGTVSGDSGVIESVGYPTRLYANNVFCQWHIQGLPGHYLTIRFEDFNLQSSPGCAKDFVEIWENHTSGILLGRYCGNSIPSSVDTSSNVASIRFVTDGSVTDSGFRLQFKSSREVCGGDLHGPTGTFTSPNYPNPNPHPRICEWTINVHEGRQIILTFTNLRLSTQQSCNTEHLIVFNGIRNNSPRLQKLCSRVNVTNEFKSSGNTMKVIFFTDGSRPYGGFTASYTSSEDAVCGGTLPSVSGGNFSSPGYNGIRDYARNLDCEWTLSNPNRENSSISIHFLGLSLESHQDCTFDVLEFRVGNADGPLIEKFCSLSAPRVPLVIPYPQVWIHFVSNERVEYTGFYVEYSFTNCGGIQTGENGVISSPNYPNLYSRWTQCSWLLEAPEGHTITLTFSDFSVENHPTCTSDSVTVRNGDSPGSPIIGRYCGQSVPGPIQSGSNQLVVTFNTNNQGQSRGFYATWNTNTLGCGGTLHSDNGTIKSPHWPQTFPENSRCSWTAVTHESKHWEISFDSNFRIPSSDSQCRNSFVKVWEGMLETNDALLATSCGNVAPSPIVTLGNIFTAVFQSEEMPAQGFSASFISRCGRTFNSSTGDIVSPNFPKHYDNNMNCNYYIDVAPQSLVILTFVSFHLEDRSAVSGTCDYDGLHIIKGHNLSSTPLVTICGSETLRPLTIDGPVMLNFYSDAYITDFGFKISYRVANCGGIYSGTYGVLNSPSFSYTNYPNNVYCVYSLQVRNDRLILLRFNDFEIVPSNLCSHDYLEVFDGPSIGNRSIGKFCGSTLPQVIKSTNNSLTLLFKTDSSQTARGWKVSFRETIGPQQGCGGYLTEDSKSFVSPDHDSDGLYDKGLNCIWYIIAPENKLVKLTFNAFTLEEPSSPGKCTFDYVQIADGASINSYLGGRFCGSSRPAPFISSGNFLTVQFVSDISIQMRGFNATYTFVDMPCGGTYNATSMPQNTSSPQLSNIRRPFSTCTWVIEAPPHQQVQITVWKLQLPSQDCSRSSLELQDSEQTNGNQVTQFCGANYTTLPVFYSSGSTAVVVFKSDFLNRNSRVHFTYEIADCNREYNQAFGNLKSPGWPQGYANNLDCSIILRAPQNHRISLFFYWFQLEDSRQCMNDFLEVRNGSSSSSPLLGKYCSNLLPNPIFSQSNELYLHFHSDDSDTHHGYEIIWASSPTGCGGTLLGNEGILANPGFPDSYPNNTHCEWTIVAPSGRPLSVGFPFLSIDSPGGCDQNYLILFNGPDANSPPFGPFCGIDTVVAPFHASSNRVFIRFHAEYATVSSGFEIMWSS |
Gene Information
Entrez Gene ID
Gene Name
cubilin (intrinsic factor-cobalamin receptor)
Gene Symbol
Species
Mus musculus
Gene Ontology (GO Annotations)
GO ID | Source | Type | Description |
---|---|---|---|
GO:0005794 | IDA:MGI | C | Golgi apparatus |
GO:0045177 | IDA:MGI | C | apical part of cell |
GO:0016324 | IDA:MGI | C | apical plasma membrane |
GO:0005903 | IDA:MGI | C | brush border |
GO:0005905 | IDA:MGI | C | coated pit |
GO:0005737 | IDA:MGI | C | cytoplasm |
GO:0030139 | IDA:MGI | C | endocytic vesicle |
GO:0005783 | IDA:MGI | C | endoplasmic reticulum |
GO:0005768 | IDA:MGI | C | endosome |
GO:0070062 | IEA:Ensembl | C | extracellular vesicular exosome |
GO:0005764 | IEA:UniProtKB-KW | C | lysosome |
GO:0005509 | IEA:InterPro | F | calcium ion binding |
GO:0031419 | IEA:UniProtKB-KW | F | cobalamin binding |
GO:0004872 | TAS:MGI | F | receptor activity |
GO:0008203 | IEA:UniProtKB-KW | P | cholesterol metabolic process |
GO:0042953 | IDA:MGI | P | lipoprotein transport |
GO:0006898 | IMP:MGI | P | receptor-mediated endocytosis |
KEGG Pathway Links
KEGG Pathway ID | Description |
---|---|
mmu04977 | Vitamin digestion and absorption |
REACTOME Pathway Links
Domain Information
InterPro Annotations
Accession | Description |
---|---|
IPR000859 | CUB domain |
IPR028876 | Cubilin |
IPR024731 | EGF domain, merozoite surface protein 1-like |
IPR001881 | EGF-like calcium-binding domain |
IPR018097 | EGF-like calcium-binding, conserved site |
IPR013032 | EGF-like, conserved site |
IPR000152 | EGF-type aspartate/asparagine hydroxylation site |
IPR000742 | Epidermal growth factor-like domain |
UniProt Annotations
Entry Information
Gene Name
cubilin (intrinsic factor-cobalamin receptor)
Protein Entry
CUBN_MOUSE
UniProt ID
Species
Mouse
Comments
Comment Type | Description |
---|---|
Developmental Stage | Detected in yolk sac endoderm as early as day 6 and is present at the apical surface of those cells throughout the remainder of pregnancy. Apical expression is pronounced in the extraembryonic visceral endoderm (VE) of 6-9.5 dpc. Expressed by a subpopulation of cells dispersed within the 7.5 dpc embryonic endoderm and having a migratory morphology. First detected at the eight-cell stage. At the 32-cell stage expressed in all outer cells which are at the origin of the trophectoderm (TE). During the blastocyst stage, expression is predominant at the apical membrane of the TE cells. {ECO:0000269|PubMed:11856751, ECO:0000269|PubMed:14983511, ECO:0000269|PubMed:15616221}. |
Domain | The CUB domains 5 to 8 mediate binding to GIF and ALB. CUB domains 1 and 2 mediate interaction with LRP2 (By similarity). {ECO:0000250}. |
Function | Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface. {ECO:0000269|PubMed:10766831, ECO:0000269|PubMed:11278724, ECO:0000269|PubMed:11856751, ECO:0000269|PubMed:15342463}. |
Ptm | N-glycosylated. {ECO:0000269|PubMed:11856751, ECO:0000269|PubMed:19349973}. |
Ptm | The precursor is cleaved by a trans-Golgi proteinase furin. The result is a propeptide cleaved off (By similarity). {ECO:0000250}. |
Similarity | Contains 27 CUB domains. {ECO:0000255|PROSITE- ProRule:PRU00059}. |
Similarity | Contains 7 EGF-like domains. {ECO:0000255|PROSITE- ProRule:PRU00076}. |
Subcellular Location | Endosome membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Lysosome membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Note=Colocalizes with AMN and LRP2 in the endocytotic apparatus of epithelial cells. {ECO:0000250}. |
Subunit | Interacts with LRP2 in a dual-receptor complex in a calcium-dependent manner (By similarity). Component of the cubam complex composed of CUBN and AMN. The cubam complex can oligomerize and form cubam trimers. Found in a complex with PID1/PCLI1, LRP1 and CUBNI (By similarity). Interacts with LRP1 and PID1/PCLI1 (By similarity). {ECO:0000250}. |
Tissue Specificity | Expressed in kidney, thymus, ileum, placenta, small intestine and yolk sac. In kidney expressed on the apical brush border surface of proximal tubular cells, in particular in endosomes and recycling membranes vesicles, so-called dense apical tubules, which carry internalized receptors back to the cell surface. Expressed in fetal membranes of yolk sac, placenta of pregnant females. {ECO:0000269|PubMed:10766831, ECO:0000269|PubMed:11856751}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP006483 (as displayed in Record Overview)
Protein GI | Database | Accession | Length | Protein Name |
---|---|---|---|---|
124487348 | RefSeq | NP_001074553 | 3623 | cubilin precursor |
Identical Sequences to LMP006483 proteins
Reference | Database | Accession | Length | Protein Name |
---|---|---|---|---|
GI:124487348 | SwissProt | Q9JLB4.3 | 3623 | RecName: Full=Cubilin; AltName: Full=Intrinsic factor-cobalamin receptor; Flags: Precursor [Mus musculus] |
Related Sequences to LMP006483 proteins
Reference | Database | Accession | Length | Protein Name |
---|---|---|---|---|
GI:124487348 | GenBank | AAC71661.1 | 3623 | intrinsic factor-B12 receptor precursor [Rattus norvegicus] |
GI:124487348 | GenBank | AAQ47042.1 | 3623 | Sequence 2 from patent US 6586389 |
GI:124487348 | GenBank | EDL78718.1 | 3623 | cubilin (intrinsic factor-cobalamin receptor), isoform CRA_b [Rattus norvegicus] |
GI:124487348 | RefSeq | NP_445784.1 | 3623 | cubilin precursor [Rattus norvegicus] |
GI:124487348 | RefSeq | XP_006985998.1 | 3620 | PREDICTED: cubilin [Peromyscus maniculatus bairdii] |
GI:124487348 | SwissProt | O70244.2 | 3623 | RecName: Full=Cubilin; AltName: Full=460 kDa receptor; AltName: Full=Glycoprotein 280; Short=gp280; AltName: Full=Intrinsic factor-cobalamin receptor; AltName: Full=Intrinsic factor-vitamin B12 receptor; Flags: Precursor [Rattus norvegicus] |