Gene/Proteome Database (LMPD)
LMPD ID
LMP006618
Gene ID
Species
Homo sapiens (Human)
Gene Name
TTK protein kinase
Gene Symbol
Synonyms
CT96; ESK; MPH1; MPS1; MPS1L1; PYT
Alternate Names
dual specificity protein kinase TTK; cancer/testis antigen 96; monopolar spindle 1 kinase; monopolar spindle 1-like 1; phosphotyrosine picked threonine kinase; phosphotyrosine picked threonine-protein kinase
Chromosome
6
Map Location
6q14.1
EC Number
2.7.12.1
Summary
This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2009]
Orthologs
Proteins
| dual specificity protein kinase TTK isoform 1 | |
|---|---|
| Refseq ID | NP_003309 |
| Protein GI | 23308722 |
| UniProt ID | P33981 |
| mRNA ID | NM_003318 |
| Length | 857 |
| RefSeq Status | REVIEWED |
| MESEDLSGRELTIDSIMNKVRDIKNKFKNEDLTDELSLNKISADTTDNSGTVNQIMMMANNPEDWLSLLLKLEKNSVPLSDALLNKLIGRYSQAIEALPPDKYGQNESFARIQVRFAELKAIQEPDDARDYFQMARANCKKFAFVHISFAQFELSQGNVKKSKQLLQKAVERGAVPLEMLEIALRNLNLQKKQLLSEEEKKNLSASTVLTAQESFSGSLGHLQNRNNSCDSRGQTTKARFLYGENMPPQDAEIGYRNSLRQTNKTKQSCPFGRVPVNLLNSPDCDVKTDDSVVPCFMKRQTSRSECRDLVVPGSKPSGNDSCELRNLKSVQNSHFKEPLVSDEKSSELIITDSITLKNKTESSLLAKLEETKEYQEPEVPESNQKQWQSKRKSECINQNPAASSNHWQIPELARKVNTEQKHTTFEQPVFSVSKQSPPISTSKWFDPKSICKTPSSNTLDDYMSCFRTPVVKNDFPPACQLSTPYGQPACFQQQQHQILATPLQNLQVLASSSANECISVKGRIYSILKQIGSGGSSKVFQVLNEKKQIYAIKYVNLEEADNQTLDSYRNEIAYLNKLQQHSDKIIRLYDYEITDQYIYMVMECGNIDLNSWLKKKKSIDPWERKSYWKNMLEAVHTIHQHGIVHSDLKPANFLIVDGMLKLIDFGIANQMQPDTTSVVKDSQVGTVNYMPPEAIKDMSSSRENGKSKSKISPKSDVWSLGCILYYMTYGKTPFQQIINQISKLHAIIDPNHEIEFPDIPEKDLQDVLKCCLKRDPKQRISIPELLAHPYVQIQTHPVNQMAKGTTEEMKYVLGQLVGLNSPNSILKAAKTLYEHYSGGESHNSSSSKTFEKKRGKK | |
| dual specificity protein kinase TTK isoform 2 | |
|---|---|
| Refseq ID | NP_001160163 |
| Protein GI | 262399361 |
| UniProt ID | P33981 |
| mRNA ID | NM_001166691 |
| Length | 856 |
| RefSeq Status | REVIEWED |
| MESEDLSGRELTIDSIMNKVRDIKNKFKNEDLTDELSLNKISADTTDNSGTVNQIMMMANNPEDWLSLLLKLEKNSVPLSDALLNKLIGRYSQAIEALPPDKYGQNESFARIQVRFAELKAIQEPDDARDYFQMARANCKKFAFVHISFAQFELSQGNVKKSKQLLQKAVERGAVPLEMLEIALRNLNLQKKQLLSEEEKKNLSASTVLTAQESFSGSLGHLQNRNNSCDSRGQTTKARFLYGENMPPQDAEIGYRNSLRQTNKTKQSCPFGRVPVNLLNSPDCDVKTDDSVVPCFMKRQTSRSECRDLVVPGSKPSGNDSCELRNLKSVQNSHFKEPLVSDEKSSELIITDSITLKNKTESSLLAKLEETKEYQEPEVPESNQKQWQSKRKSECINQNPAASSNHWQIPELARKVNTEKHTTFEQPVFSVSKQSPPISTSKWFDPKSICKTPSSNTLDDYMSCFRTPVVKNDFPPACQLSTPYGQPACFQQQQHQILATPLQNLQVLASSSANECISVKGRIYSILKQIGSGGSSKVFQVLNEKKQIYAIKYVNLEEADNQTLDSYRNEIAYLNKLQQHSDKIIRLYDYEITDQYIYMVMECGNIDLNSWLKKKKSIDPWERKSYWKNMLEAVHTIHQHGIVHSDLKPANFLIVDGMLKLIDFGIANQMQPDTTSVVKDSQVGTVNYMPPEAIKDMSSSRENGKSKSKISPKSDVWSLGCILYYMTYGKTPFQQIINQISKLHAIIDPNHEIEFPDIPEKDLQDVLKCCLKRDPKQRISIPELLAHPYVQIQTHPVNQMAKGTTEEMKYVLGQLVGLNSPNSILKAAKTLYEHYSGGESHNSSSSKTFEKKRGKK | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0016020 | IDA:UniProtKB | C | membrane |
| GO:0005819 | TAS:ProtInc | C | spindle |
| GO:0005524 | IEA:UniProtKB-KW | F | ATP binding |
| GO:0004674 | TAS:ProtInc | F | protein serine/threonine kinase activity |
| GO:0004712 | IEA:UniProtKB-EC | F | protein serine/threonine/tyrosine kinase activity |
| GO:0004713 | TAS:ProtInc | F | protein tyrosine kinase activity |
| GO:0051304 | IEA:InterPro | P | chromosome separation |
| GO:0007094 | TAS:ProtInc | P | mitotic spindle assembly checkpoint |
| GO:0007052 | TAS:ProtInc | P | mitotic spindle organization |
| GO:0018108 | TAS:GOC | P | peptidyl-tyrosine phosphorylation |
| GO:0008284 | TAS:ProtInc | P | positive regulation of cell proliferation |
| GO:0010862 | TAS:BHF-UCL | P | positive regulation of pathway-restricted SMAD protein phosphorylation |
| GO:0007051 | TAS:ProtInc | P | spindle organization |
KEGG Pathway Links
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR027084 | Dual specificity protein kinase TTK |
| IPR000719 | Protein kinase domain |
| IPR017441 | Protein kinase, ATP binding site |
| IPR011009 | Protein kinase-like domain |
| IPR008271 | Serine/threonine-protein kinase, active site |
| IPR002290 | Serine/threonine/dual specificity protein kinase, catalytic domain |
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P33981-1; Sequence=Displayed; Name=2; IsoId=P33981-2; Sequence=VSP_043072; Note=No experimental confirmation available.; |
| Catalytic Activity | ATP + a protein = ADP + a phosphoprotein. |
| Enzyme Regulation | Inhibited by the ATP-competitive kinase inhibitor, SP600125. |
| Function | Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint. |
| Sequence Caution | Sequence=AAA61239.1; Type=Erroneous initiation; Evidence= ; Sequence=CAB87580.1; Type=Erroneous initiation; Evidence= ; |
| Similarity | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. |
| Similarity | Contains 1 protein kinase domain. |
| Subunit | Interacts with TPR; the interactions occurs in a microtubule-independent manner. {ECO |
| Tissue Specificity | Present in rapidly proliferating cell lines. |
Identical and Related Proteins
Unique RefSeq proteins for LMP006618 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 23308722 | RefSeq | NP_003309 | 857 | dual specificity protein kinase TTK isoform 1 |
| 262399361 | RefSeq | NP_001160163 | 856 | dual specificity protein kinase TTK isoform 2 |
Identical Sequences to LMP006618 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:262399361 | DBBJ | BAF85149.1 | 856 | unnamed protein product [Homo sapiens] |
| GI:23308722 | GenBank | AHD80026.1 | 857 | Sequence 30973 from patent US 8586006 |
| GI:23308722 | GenBank | AHE17212.1 | 857 | Sequence 2 from patent US 8614176 |
| GI:23308722 | GenBank | AHH75921.1 | 857 | Sequence 16 from patent US 8623829 |
| GI:23308722 | GenBank | AIC49915.1 | 857 | TTK, partial [synthetic construct] |
| GI:23308722 | GenBank | AIC55278.1 | 857 | TTK, partial [synthetic construct] |
| GI:23308722 | GenBank | AIC62615.1 | 857 | TTK, partial [synthetic construct] |
Related Sequences to LMP006618 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:23308722 | DBBJ | BAF85149.1 | 856 | unnamed protein product [Homo sapiens] |
| GI:262399361 | EMBL | CCO75436.1 | 857 | unnamed protein product [Homo sapiens] |
| GI:262399361 | EMBL | CCO75438.1 | 857 | unnamed protein product [Homo sapiens] |
| GI:262399361 | EMBL | CCQ77693.1 | 857 | unnamed protein product [Homo sapiens] |
| GI:262399361 | EMBL | CCQ77702.1 | 857 | unnamed protein product [Homo sapiens] |
| GI:262399361 | EMBL | CCQ77711.1 | 857 | unnamed protein product [Homo sapiens] |
| GI:262399361 | GenBank | ADT57159.1 | 857 | Sequence 2 from patent US 7847060 |
| GI:23308722 | GenBank | JAA16122.1 | 857 | TTK protein kinase [Pan troglodytes] |
| GI:23308722 | GenBank | JAA25466.1 | 857 | TTK protein kinase [Pan troglodytes] |
| GI:23308722 | GenBank | JAA44082.1 | 857 | TTK protein kinase [Pan troglodytes] |
| GI:23308722 | RefSeq | XP_518603.2 | 857 | PREDICTED: dual specificity protein kinase TTK isoform X1 [Pan troglodytes] |
| GI:23308722 | RefSeq | NP_001160163.1 | 856 | dual specificity protein kinase TTK isoform 2 [Homo sapiens] |