Gene/Proteome Database (LMPD)
LMPD ID
LMP006674
Gene ID
Species
Homo sapiens (Human)
Gene Name
phospholipase C, delta 3
Gene Symbol
Synonyms
PLC-delta-3
Alternate Names
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3; PLC delta3; phosphoinositide phospholipase C-delta-3; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-3
Chromosome
17
Map Location
17q21.31
EC Number
3.1.4.11
Summary
This gene encodes a member of the phospholipase C family, which catalyze the hydrolysis of phosphatidylinositol 4,5-bisphosphate to generate the second messengers diacylglycerol and inositol 1,4,5-trisphosphate (IP3). Diacylglycerol and IP3 mediate a variety of cellular responses to extracellular stimuli by inducing protein kinase C and increasing cytosolic Ca(2+) concentrations. This enzyme localizes to the plasma membrane and requires calcium for activation. Its activity is inhibited by spermine, sphingosine, and several phospholipids. [provided by RefSeq, Jul 2008]
Orthologs
Proteins
| 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3 | |
|---|---|
| Refseq ID | NP_588614 |
| Protein GI | 19115964 |
| UniProt ID | Q8N3E9 |
| mRNA ID | NM_133373 |
| Length | 789 |
| RefSeq Status | REVIEWED |
| MLCGRWRRCRRPPEEPPVAAQVAAQVAAPVALPSPPTPSDGGTKRPGLRALKKMGLTEDEDVRAMLRGSRLRKIRSRTWHKERLYRLQEDGLSVWFQRRIPRAPSQHIFFVQHIEAVREGHQSEGLRRFGGAFAPARCLTIAFKGRRKNLDLAAPTAEEAQRWVRGLTKLRARLDAMSQRERLDHWIHSYLHRADSNQDSKMSFKEIKSLLRMVNVDMNDMYAYLLFKECDHSNNDRLEGAEIEEFLRRLLKRPELEEIFHQYSGEDRVLSAPELLEFLEDQGEEGATLARAQQLIQTYELNETAKQHELMTLDGFMMYLLSPEGAALDNTHTCVFQDMNQPLAHYFISSSHNTYLTDSQIGGPSSTEAYVRAFAQGCRCVELDCWEGPGGEPVIYHGHTLTSKILFRDVVQAVRDHAFTLSPYPVILSLENHCGLEQQAAMARHLCTILGDMLVTQALDSPNPEELPSPEQLKGRVLVKGKKLPAARSEDGRALSDREEEEEDDEEEEEEVEAAAQRRLAKQISPELSALAVYCHATRLRTLHPAPNAPQPCQVSSLSERKAKKLIREAGNSFVRHNARQLTRVYPLGLRMNSANYSPQEMWNSGCQLVALNFQTPGYEMDLNAGRFLVNGQCGYVLKPACLRQPDSTFDPEYPGPPRTTLSIQVLTAQQLPKLNAEKPHSIVDPLVRIEIHGVPADCARQETDYVLNNGFNPRWGQTLQFQLRAPELALVRFVVEDYDATSPNDFVGQFTLPLSSLKQGYRHIHLLSKDGASLSPATLFIQIRIQRS | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005829 | IEA:Ensembl | C | cytosol |
| GO:0005886 | TAS:Reactome | C | plasma membrane |
| GO:0005509 | IEA:InterPro | F | calcium ion binding |
| GO:0004435 | IEA:UniProtKB-EC | F | phosphatidylinositol phospholipase C activity |
| GO:0004871 | IEA:UniProtKB-KW | F | signal transducer activity |
| GO:0001525 | IEA:Ensembl | P | angiogenesis |
| GO:0043647 | TAS:Reactome | P | inositol phosphate metabolic process |
| GO:0035556 | IEA:InterPro | P | intracellular signal transduction |
| GO:0060716 | IEA:Ensembl | P | labyrinthine layer blood vessel development |
| GO:0016042 | IEA:UniProtKB-KW | P | lipid catabolic process |
| GO:0042127 | IEA:Ensembl | P | regulation of cell proliferation |
| GO:0044281 | TAS:Reactome | P | small molecule metabolic process |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| hsa04020 | Calcium signaling pathway |
| hsa04919 | Thyroid hormone signaling pathway |
BIOCYC Pathway Links
| BIOCYC Pathway ID | Description |
|---|---|
| PWY-6351 | D-myo-inositol (1,4,5)-trisphosphate biosynthesis |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| REACT_150312 | Synthesis of IP3 and IP4 in the cytosol |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR028406 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3 |
| IPR000008 | C2 domain |
| IPR018247 | EF-Hand 1, calcium-binding site |
| IPR011992 | EF-hand domain pair |
| IPR017946 | PLC-like phosphodiesterase, TIM beta/alpha-barrel domain |
| IPR001192 | Phosphoinositide phospholipase C family |
| IPR000909 | Phospholipase C, phosphatidylinositol-specific , X domain |
| IPR001711 | Phospholipase C, phosphatidylinositol-specific, Y domain |
| IPR015359 | Phospholipase C, phosphoinositol-specific, EF-hand-like |
| IPR001849 | Pleckstrin homology domain |
| IPR011993 | Pleckstrin homology-like domain |
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Biophysicochemical Properties | Kinetic parameters: KM=105.3 uM for PIP2 ; Vmax=28.5 umol/min/mg enzyme ; |
| Catalytic Activity | 1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol. |
| Cofactor | Name=Ca(2+); Xref=ChEBI |
| Domain | The C2 domain is a Ca(2+)-dependent membrane-targeting module. |
| Domain | The PH domain mediates interaction with the surface membrane by binding to PIP2. |
| Enzyme Regulation | Strongly activated by phosphatidic acid. Inhibited by phosphatidylethanolamine (PtdEtn), phosphatidylcholine (PtdCho), sphingomyelin and phosphatidylserine (PtdSer). {ECO |
| Function | Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Essential for trophoblast and placental development. May participate in cytokinesis by hydrolyzing PIP2 at the cleavage furrow. |
| Induction | Down-regulated by Ca(2+) and cAMP. |
| Similarity | Contains 1 C2 domain. {ECO |
| Similarity | Contains 1 PH domain. |
| Similarity | Contains 1 PI-PLC X-box domain. {ECO |
| Similarity | Contains 1 PI-PLC Y-box domain. {ECO |
| Similarity | Contains 3 EF-hand domains. |
| Subcellular Location | Membrane; Peripheral membrane protein. Cytoplasm. Cleavage furrow. Note=Localizes at the cleavage furrow during cytokinesis. |
| Tissue Specificity | Present in corneal epithelial cells (at protein level). |
Identical and Related Proteins
Unique RefSeq proteins for LMP006674 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 19115964 | RefSeq | NP_588614 | 789 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3 |
Identical Sequences to LMP006674 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:19115964 | EMBL | CBU87821.1 | 789 | unnamed protein product [Homo sapiens] |
| GI:19115964 | EMBL | CBU96768.1 | 789 | unnamed protein product [Homo sapiens] |
| GI:19115964 | GenBank | AED62132.1 | 789 | Sequence 13 from patent US 7902154 |
| GI:19115964 | GenBank | AGX54756.1 | 789 | Sequence 9868 from patent US 8541208 |
| GI:19115964 | GenBank | AGX64196.1 | 789 | Sequence 28502 from patent US 8541208 |
| GI:19115964 | GenBank | AIC57532.1 | 789 | PLCD3, partial [synthetic construct] |
Related Sequences to LMP006674 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:19115964 | EMBL | CAD39054.2 | 777 | hypothetical protein, partial [Homo sapiens] |
| GI:19115964 | RefSeq | XP_001115230.1 | 785 | PREDICTED: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-3-like isoform 1 [Macaca mulatta] |
| GI:19115964 | RefSeq | XP_002800595.1 | 785 | PREDICTED: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-3-like isoform 2 [Macaca mulatta] |
| GI:19115964 | RefSeq | XP_003913243.1 | 785 | PREDICTED: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3 isoform X1 [Papio anubis] |
| GI:19115964 | RefSeq | XP_009430250.1 | 789 | PREDICTED: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3 [Pan troglodytes] |
| GI:19115964 | RefSeq | XP_010352314.1 | 785 | PREDICTED: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3 isoform X1 [Rhinopithecus roxellana] |