Gene/Proteome Database (LMPD)

LMPD ID
LMP006735
Gene ID
56624
Species
Homo sapiens (Human)
Gene Name
N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
Gene Symbol
ASAH2
Synonyms
BCDase; HNAC1; LCDase; N-CDase; NCDase
Alternate Names
neutral ceramidase; mitochondrial ceramidase; non-lysosomal ceramidase; acylsphingosine deacylase 2; neutral/alkaline ceramidase; N-acylsphingosine amidohydrolase 2
Chromosome
10
Map Location
10q11.21
EC Number
3.5.1.23
Summary
Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).[supplied by OMIM, Mar 2008]
Orthologs
View orthologs and multiple alignments for ASAH2

Proteins

neutral ceramidase isoform a
Refseq ID NP_063946
Protein GI 221218981
UniProt ID Q9NR71
mRNA ID NM_019893
Length 780
RefSeq Status VALIDATED
MAKRTFSNLETFLIFLLVMMSAITVALLSLLFITSGTIENHKDLGGHFFSTTQSPPATQGSTAAQRSTATQHSTATQSSTATQTSPVPLTPESPLFQNFSGYHIGVGRADCTGQVADINLMGYGKSGQNAQGILTRLYSRAFIMAEPDGSNRTVFVSIDIGMVSQRLRLEVLNRLQSKYGSLYRRDNVILSGTHTHSGPAGYFQYTVFVIASEGFSNQTFQHMVTGILKSIDIAHTNMKPGKIFINKGNVDGVQINRSPYSYLQNPQSERARYSSNTDKEMIVLKMVDLNGDDLGLISWFAIHPVSMNNSNHLVNSDNVGYASYLLEQEKNKGYLPGQGPFVAAFASSNLGDVSPNILGPRCINTGESCDNANSTCPIGGPSMCIAKGPGQDMFDSTQIIGRAMYQRAKELYASASQEVTGPLASAHQWVDMTDVTVWLNSTHASKTCKPALGYSFAAGTIDGVGGLNFTQGKTEGDPFWDTIRDQILGKPSEEIKECHKPKPILLHTGELSKPHPWHPDIVDVQIITLGSLAITAIPGEFTTMSGRRLREAVQAEFASHGMQNMTVVISGLCNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRNLAKAIATDTVANLSRGPEPPFFKQLIVPLIPSIVDRAPKGRTFGDVLQPAKPEYRVGEVAEVIFVGANPKNSVQNQTHQTFLTVEKYEATSTSWQIVCNDASWETRFYWHKGLLGLSNATVEWHIPDTAQPGIYRIRYFGHNRKQDILKPAVILSFEGTSPAFEVVTI
mat_peptide: 99..780 product: Neutral ceramidase soluble form (By similarity) inference: non-experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (Q9NR71.2) calculated_mol_wt: 75074 peptide sequence: FSGYHIGVGRADCTGQVADINLMGYGKSGQNAQGILTRLYSRAFIMAEPDGSNRTVFVSIDIGMVSQRLRLEVLNRLQSKYGSLYRRDNVILSGTHTHSGPAGYFQYTVFVIASEGFSNQTFQHMVTGILKSIDIAHTNMKPGKIFINKGNVDGVQINRSPYSYLQNPQSERARYSSNTDKEMIVLKMVDLNGDDLGLISWFAIHPVSMNNSNHLVNSDNVGYASYLLEQEKNKGYLPGQGPFVAAFASSNLGDVSPNILGPRCINTGESCDNANSTCPIGGPSMCIAKGPGQDMFDSTQIIGRAMYQRAKELYASASQEVTGPLASAHQWVDMTDVTVWLNSTHASKTCKPALGYSFAAGTIDGVGGLNFTQGKTEGDPFWDTIRDQILGKPSEEIKECHKPKPILLHTGELSKPHPWHPDIVDVQIITLGSLAITAIPGEFTTMSGRRLREAVQAEFASHGMQNMTVVISGLCNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRNLAKAIATDTVANLSRGPEPPFFKQLIVPLIPSIVDRAPKGRTFGDVLQPAKPEYRVGEVAEVIFVGANPKNSVQNQTHQTFLTVEKYEATSTSWQIVCNDASWETRFYWHKGLLGLSNATVEWHIPDTAQPGIYRIRYFGHNRKQDILKPAVILSFEGTSPAFEVVTI
neutral ceramidase isoform b
Refseq ID NP_001137446
Protein GI 221307475
UniProt ID Q9NR71
mRNA ID NM_001143974
Length 745
RefSeq Status VALIDATED
MAKRTFSNLETFLIFLLVMMSAITVALLSLLFITSGTIENHKDLGGHFFSTTQSPPATQGSTAAQRSTATQHSTATQSSTATQTSPVPLTPESPLFQNFSGYHIGVGRADCTGQVADINLMGYGKSGQNAQGILTRLYSRAFIMAEPDGSNRTVFVSIDIGMVSQRLRLEVLNRLQSKYGSLYRRDNVILSGTHTHSGPAGYFQYTVFVIASEGFSNQTFQHMVTGILKSIDIAHTNMKPGKIFINKGNVDGVQINRSPYSYLQNPQSERARYSSNTDKEMIVLKMVDLNGDDLGLISWFAIHPVSMNNSNHLVNSDNVGYASYLLEQEKNKGYLPGQGPFVAAFASSNLGDVSPNILGPRCINTGESCDNANSTCPIGGPSMCIAKGPGQDMFDSTQIIGRAMYQRAKSKTCKPALGYSFAAGTIDGVGGLNFTQGKTEGDPFWDTIRDQILGKPSEEIKECHKPKPILLHTGELSKPHPWHPDIVDVQIITLGSLAITAIPGEFTTMSGRRLREAVQAEFASHGMQNMTVVISGLCNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRNLAKAIATDTVANLSRGPEPPFFKQLIVPLIPSIVDRAPKGRTFGDVLQPAKPEYRVGEVAEVIFVGANPKNSVQNQTHQTFLTVEKYEATSTSWQIVCNDASWETRFYWHKGLLGLSNATVEWHIPDTAQPGIYRIRYFGHNRKQDILKPAVILSFEGTSPAFEVVTI

Gene Information

Entrez Gene ID
56624
Gene Name
N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
Gene Symbol
ASAH2
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0005739 TAS:ProtInc C mitochondrion
GO:0005886 TAS:Reactome C plasma membrane
GO:0017040 IEA:UniProtKB-EC F ceramidase activity
GO:0006915 IEA:UniProtKB-KW P apoptotic process
GO:0006672 TAS:ProtInc P ceramide metabolic process
GO:0006687 TAS:Reactome P glycosphingolipid metabolic process
GO:0007165 TAS:ProtInc P signal transduction
GO:0044281 TAS:Reactome P small molecule metabolic process
GO:0006665 TAS:Reactome P sphingolipid metabolic process

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY3DJ-11470 sphingosine and sphingosine-1-phosphate metabolism

REACTOME Pathway Links

REACTOME Pathway ID Description
REACT_116105 Glycosphingolipid metabolism

Domain Information

InterPro Annotations

Accession Description
IPR006823 Neutral/alkaline nonlysosomal ceramidase

UniProt Annotations

Entry Information

Gene Name
N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
Protein Entry
ASAH2_HUMAN
UniProt ID
Q9NR71
Species
Human

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9NR71-1; Sequence=Displayed; Name=2; IsoId=Q9NR71-2; Sequence=VSP_019928; Note=No experimental confirmation available.;
Biophysicochemical Properties Kinetic parameters: KM=71.4 uM for octanoyl-sphingosine ; KM=66 uM for palmitoyl-sphingosine {ECO
Catalytic Activity N-acylsphingosine + H(2)O = a carboxylate + sphingosine. {ECO
Enzyme Regulation Inhibited by dithiothreitol (DTT), 2- mercaptoethanol, Zn(2+), Cu(2+) and Fe(2+). Enhanced by Na(+) and Ca(2+), and at lower level Mg(2+) and Mn(2+).
Function Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. {ECO
Ptm N-glycosylated. Required for enzyme activity (By similarity).
Ptm O-glycosylated. Required to retain it as a type II membrane protein at the cell surface.
Ptm Phosphorylated. May prevent ubiquitination and subsequent degradation (By similarity).
Ptm Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by nitric oxid (By similarity).
Sequence Caution Sequence=AAL06061.1; Type=Erroneous initiation; Evidence= ; Sequence=CAI15870.1; Type=Erroneous initiation; Evidence= ;
Similarity Belongs to the neutral ceramidase family.
Subcellular Location Cell membrane ; Single-pass type II membrane protein . Note=The neutral ceramidase soluble form is a secreted protein. According to PubMed:10781606, it is mitochondrial. However, they used a shorter form in its N- terminus, which may explain this localization which probably does not exist in vivo.
Tissue Specificity Primarily expressed in the intestine (PubMed:17334805). Ubiquitously expressed with higher levels in kidney, skeletal muscle and heart (PubMed:10781606). According to PubMed:17334805, ubiquitous expression attributed to ASAH2 may be actually that of the paralog ASAH2B. {ECO

Identical and Related Proteins

Unique RefSeq proteins for LMP006735 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
221218981 RefSeq NP_063946 780 neutral ceramidase isoform a
221307475 RefSeq NP_001137446 745 neutral ceramidase isoform b

Identical Sequences to LMP006735 proteins

Reference Database Accession Length Protein Name
GI:221218981 GenBank AAQ04667.2 780 neutral/alkaline ceramidase [Homo sapiens]
GI:221218981 SwissProt Q9NR71.2 780 RecName: Full=Neutral ceramidase; Short=N-CDase; Short=NCDase; AltName: Full=Acylsphingosine deacylase 2; AltName: Full=BCDase; AltName: Full=LCDase; Short=hCD; AltName: Full=N-acylsphingosine amidohydrolase 2; AltName: Full=Non-lysosomal ceramidase; Contains: RecName: Full=Neutral ceramidase soluble form [Homo sapiens]

Related Sequences to LMP006735 proteins

Reference Database Accession Length Protein Name
GI:221218981 EMBL CAC60194.1 761 unnamed protein product [Homo sapiens]
GI:221218981 GenBank AAF86240.1 761 mitochondrial ceramidase [Homo sapiens]
GI:221218981 GenBank AAL06061.1 761 mitochondrial ceramidase [Homo sapiens]
GI:221307475 GenBank AAI07106.1 726 ASAH2 protein [Homo sapiens]
GI:221307475 GenBank AAQ04667.2 780 neutral/alkaline ceramidase [Homo sapiens]
GI:221218981 GenBank AHE02196.1 761 Sequence 60550 from patent US 8586006
GI:221218981 RefSeq XP_507791.2 780 PREDICTED: neutral ceramidase isoform X1 [Pan troglodytes]
GI:221307475 RefSeq XP_507791.2 780 PREDICTED: neutral ceramidase isoform X1 [Pan troglodytes]
GI:221307475 RefSeq NP_063946.2 780 neutral ceramidase isoform a [Homo sapiens]
GI:221307475 RefSeq XP_009456735.1 780 PREDICTED: neutral ceramidase isoform X1 [Pan troglodytes]
GI:221218981 RefSeq XP_009456735.1 780 PREDICTED: neutral ceramidase isoform X1 [Pan troglodytes]
GI:221307475 SwissProt Q9NR71.2 780 RecName: Full=Neutral ceramidase; Short=N-CDase; Short=NCDase; AltName: Full=Acylsphingosine deacylase 2; AltName: Full=BCDase; AltName: Full=LCDase; Short=hCD; AltName: Full=N-acylsphingosine amidohydrolase 2; AltName: Full=Non-lysosomal ceramidase; Contains: RecName: Full=Neutral ceramidase soluble form [Homo sapiens]