|
Comment Type
|
Description
|
|
Biophysicochemical Properties
|
Kinetic parameters: KM=34 mM for p-nitrophenylphosphate {ECO:0000269|PubMed:16442612}; pH dependence: Optimum pH is 7.8. {ECO:0000269|PubMed:16442612};
|
|
Biophysicochemical Properties
|
Kinetic parameters: KM=34 mM for p-nitrophenylphosphate ; pH dependence: Optimum pH is 7.8. ;
|
|
Catalytic Activity
|
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate.
|
|
Cofactor
|
Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence= ; Note=Binds 2 manganese ions per subunit. ;
|
|
Cofactor
|
Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250}; Note=Binds 2 manganese ions per subunit. {ECO:0000250};
|
|
Domain
|
The TPR repeats mediate protein-protein interactions with substrate proteins, but also autoinhibit PPT1 phosphatase activity.
|
|
Enzyme Regulation
|
Stimulated by arachidonic acid and other unsaturated fatty acids, and by arachidoyl coenzyme A
|
|
Enzyme Regulation
|
Stimulated by arachidonic acid and other unsaturated fatty acids, and by arachidoyl coenzyme A. {ECO:0000269|PubMed:12694636}.
|
|
Function
|
Protein phosphatase that specifically binds to and dephosphorylates the molecular chaperone Hsp90 (HSC82 and HSP82). Dephosphorylation positively regulates the Hsp90 chaperone machinery
|
|
Function
|
Protein phosphatase that specifically binds to and dephosphorylates the molecular chaperone Hsp90 (HSC82 and HSP82). Dephosphorylation positively regulates the Hsp90 chaperone machinery. {ECO:0000269|PubMed:16407978}.
|
|
Induction
|
Expression peaks in early log phase and decreases dramatically during the stationary phase (at protein level)
|
|
Induction
|
Expression peaks in early log phase and decreases dramatically during the stationary phase (at protein level). {ECO:0000269|PubMed:12694636}.
|
|
Interaction
|
P15108:HSC82; NbExp=3; IntAct=EBI-13796, EBI-8666; P02829:HSP82; NbExp=8; IntAct=EBI-13796, EBI-8659;
|
|
Miscellaneous
|
Present with 6990 molecules/cell in log phase SD medium
|
|
Miscellaneous
|
Present with 6990 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
|
|
Sequence Caution
|
Sequence=CAA61596.1; Type=Frameshift; Positions=381; Evidence= ;
|
|
Sequence Caution
|
Sequence=CAA61596.1; Type=Frameshift; Positions=381; Evidence={ECO:0000305};
|
|
Similarity
|
Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily
|
|
Similarity
|
Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily. {ECO:0000305}.
|
|
Similarity
|
Contains 3 TPR repeats. {ECO:0000255|PROSITE- ProRule:PRU00339}.
|
|
Subcellular Location
|
Nucleus {ECO:0000269|PubMed:12694636, ECO:0000269|PubMed:14562095}.
|
|
Subunit
|
Interacts (via TPR repeats) with HSP82 (via C-terminal MEEVD pentapeptide)
|
|
Subunit
|
Interacts (via TPR repeats) with HSP82 (via C-terminal MEEVD pentapeptide). {ECO:0000269|PubMed:16407978}.
|