Gene/Proteome Database (LMPD)
Proteins
| Ncp1p | |
|---|---|
| Refseq ID | NP_011908 |
| Protein GI | 6321832 |
| UniProt ID | P16603 |
| mRNA ID | NM_001179172 |
| Length | 691 |
| RefSeq Status | PROVISIONAL |
| MPFGIDNTDFTVLAGLVLAVLLYVKRNSIKELLMSDDGDITAVSSGNRDIAQVVTENNKNYLVLYASQTGTAEDYAKKFSKELVAKFNLNVMCADVENYDFESLNDVPVIVSIFISTYGEGDFPDGAVNFEDFICNAEAGALSNLRYNMFGLGNSTYEFFNGAAKKAEKHLSAAGAIRLGKLGEADDGAGTTDEDYMAWKDSILEVLKDELHLDEQEAKFTSQFQYTVLNEITDSMSLGEPSAHYLPSHQLNRNADGIQLGPFDLSQPYIAPIVKSRELFSSNDRNCIHSEFDLSGSNIKYSTGDHLAVWPSNPLEKVEQFLSIFNLDPETIFDLKPLDPTVKVPFPTPTTIGAAIKHYLEITGPVSRQLFSSLIQFAPNADVKEKLTLLSKDKDQFAVEITSKYFNIADALKYLSDGAKWDTVPMQFLVESVPQMTPRYYSISSSSLSEKQTVHVTSIVENFPNPELPDAPPVVGVTTNLLRNIQLAQNNVNIAETNLPVHYDLNGPRKLFANYKLPVHVRRSNFRLPSNPSTPVIMIGPGTGVAPFRGFIRERVAFLESQKKGGNNVSLGKHILFYGSRNTDDFLYQDEWPEYAKKLDGSFEMVVAHSRLPNTKKVYVQDKLKDYEDQVFEMINNGAFIYVCGDAKGMAKGVSTALVGILSRGKSITTDEATELIKMLKTSGRYQEDVW | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005783 | IEA:UniProtKB-KW | C | endoplasmic reticulum |
| GO:0016021 | IEA:UniProtKB-KW | C | integral component of membrane |
| GO:0005741 | IEA:UniProtKB-KW | C | mitochondrial outer membrane |
| GO:0005886 | IEA:UniProtKB-KW | C | plasma membrane |
| GO:0010181 | IEA:InterPro | F | FMN binding |
| GO:0003958 | IDA:SGD | F | NADPH-hemoprotein reductase activity |
| GO:0009055 | IDA:SGD | F | electron carrier activity |
| GO:0005506 | IEA:InterPro | F | iron ion binding |
| GO:0006696 | IMP:SGD | P | ergosterol biosynthetic process |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR003097 | FAD-binding, type 1 |
| IPR017927 | Ferredoxin reductase-type FAD-binding domain |
| IPR001094 | Flavodoxin |
| IPR008254 | Flavodoxin/nitric oxide synthase |
| IPR001709 | Flavoprotein pyridine nucleotide cytochrome reductase |
| IPR029039 | Flavoprotein-like |
| IPR023208 | NADPH-cytochrome P450 reductase |
| IPR023173 | NADPH-cytochrome p450 reductase, FAD-binding, alpha-helical domain-3 |
| IPR001433 | Oxidoreductase FAD/NAD(P)-binding |
| IPR017938 | Riboflavin synthase-like beta-barrel |
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Biophysicochemical Properties | Kinetic parameters: KM=1.59 uM for cytochrome c {ECO:0000269|PubMed:11485306}; KM=1.46 uM for NADPH {ECO:0000269|PubMed:11485306}; Note=The Vmax of the reaction is 721 pmol/min/pmol enzyme towards cytochrome c, and 662 pmol/min/pmol enzyme toward NADPH.; |
| Catalytic Activity | NADPH + n oxidized hemoprotein = NADP(+) + n reduced hemoprotein. {ECO:0000269|PubMed:11485306, ECO:0000269|PubMed:9368374, ECO:0000269|PubMed:9468503}. |
| Cofactor | Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000269|PubMed:9468503}; Note=Binds 1 FAD per monomer. {ECO:0000269|PubMed:9468503}; |
| Cofactor | Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000269|PubMed:9468503}; Note=Binds 1 FMN per monomer. {ECO:0000269|PubMed:9468503}; |
| Disruption Phenotype | Accumulates 20% of ergosterol of wild type. {ECO:0000269|PubMed:9468503}. |
| Function | This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis. Has NADPH-dependent ferrireductase activity on the plasma membrane. {ECO:0000269|PubMed:11485306, ECO:0000269|PubMed:1730736, ECO:0000269|PubMed:9368374, ECO:0000269|PubMed:9468503}. |
| Induction | By galactose and on the plasma membrane by iron or copper deficiency. Repressed by glucose. {ECO:0000269|PubMed:9368374}. |
| Miscellaneous | Present with 46600 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Ptm | Phosphorylated by the cyclin-CDK PCL1-PHO85. {ECO:0000269|PubMed:15082539}. |
| Similarity | Contains 1 FAD-binding FR-type domain. {ECO:0000255|PROSITE-ProRule:PRU00716}. |
| Similarity | Contains 1 flavodoxin-like domain. {ECO:0000255|PROSITE-ProRule:PRU00088}. |
| Similarity | In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. {ECO:0000305}. |
| Subcellular Location | Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion outer membrane; Single-pass membrane protein. Cell membrane; Single-pass membrane protein. Microsome. |
| Subunit | Interacts with PCL1. {ECO:0000269|PubMed:15082539, ECO:0000269|PubMed:16407065}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP007028 (as displayed in Record Overview)
Identical Sequences to LMP007028 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:6321832 | GenBank | AAB68904.1 | 691 | Ncp1p: NADP-cytochrome P450 reductase [Saccharomyces cerevisiae] |
| GI:6321832 | GenBank | AAT93110.1 | 691 | YHR042W [Saccharomyces cerevisiae] |
| GI:6321832 | GenBank | ABZ48096.1 | 691 | Sequence 22034 from patent US 7314974 |
| GI:6321832 | GenBank | EIW10193.1 | 691 | Ncp1p [Saccharomyces cerevisiae CEN.PK113-7D] |
| GI:6321832 | SwissProt | P16603.3 | 691 | RecName: Full=NADPH--cytochrome P450 reductase; Short=CPR; Short=P450R [Saccharomyces cerevisiae S288c] |
| GI:6321832 | Third Party Genbank | DAA06734.1 | 691 | TPA: Ncp1p [Saccharomyces cerevisiae S288c] |
Related Sequences to LMP007028 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:6321832 | DBBJ | BAA02936.1 | 691 | NADPH-cytochrome P450 reductase precursor [Saccharomyces cerevisiae] |
| GI:6321832 | GenBank | ABH71432.1 | 691 | Sequence 37 from patent US 7033807 |
| GI:6321832 | GenBank | EEU05779.1 | 691 | Ncp1p [Saccharomyces cerevisiae JAY291] |
| GI:6321832 | GenBank | EWG95663.1 | 691 | Ncp1p [Saccharomyces cerevisiae R103] |
| GI:6321832 | GenBank | EWH18079.1 | 691 | Ncp1p [Saccharomyces cerevisiae P283] |
| GI:6321832 | PRF | - | 691 | NADPH cytochrome P450 reductase [Saccharomyces cerevisiae] |