Gene/Proteome Database (LMPD)
Proteins
| Ale1p | |
|---|---|
| Refseq ID | NP_014818 |
| Protein GI | 6324749 |
| UniProt ID | Q08548 |
| mRNA ID | NM_001183594 |
| Length | 619 |
| RefSeq Status | PROVISIONAL |
| MYNPVDAVLTKIITNYGIDSFTLRYAICLLGSFPLNAILKRIPEKRIGLKCCFIISMSMFYLFGVLNLVSGFRTLFISTMFTYLISRFYRSKFMPHLNFMFVMGHLAINHIHAQFLNEQTQTTVDITSSQMVLAMKLTSFAWSYYDGSCTSESDFKDLTEHQKSRAVRGHPPLLKFLAYAFFYSTLLTGPSFDYADFDSWLNCEMFRDLPESKKPMRRHHPGERRQIPKNGKLALWKVVQGLAWMILSTLGMKHFPVKYVLDKDGFPTRSFIFRIHYLFLLGFIHRFKYYAAWTISEGSCILCGLGYNGYDSKTQKIRWDRVRNIDIWTVETAQNTREMLEAWNMNTNKWLKYSVYLRVTKKGKKPGFRSTLFTFLTSAFWHGTRPGYYLTFATGALYQTCGKIYRRNFRPIFLREDGVTPLPSKKIYDLVGIYAIKLAFGYMVQPFIILDLKPSLMVWGSVYFYVHIIVAFSFFLFRGPYAKQVTEFFKSKQPKEIFIRKQKKLEKDISASSPNLGGILKAKIEHEKGKTAEEEEMNLGIPPIELEKWDNAKEDWEDFCKDYKEWRNKNGLEIEEENLSKAFERFKQEFSNAASGSGERVRKMSFSGYSPKPISKKEE | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005783 | IDA:SGD | C | endoplasmic reticulum |
| GO:0016021 | IEA:UniProtKB-KW | C | integral component of membrane |
| GO:0003841 | IMP:SGD | F | 1-acylglycerol-3-phosphate O-acyltransferase activity |
| GO:0047184 | IMP:SGD | F | 1-acylglycerophosphocholine O-acyltransferase activity |
| GO:0008374 | IDA:SGD | F | O-acyltransferase activity |
| GO:0046474 | IMP:SGD | P | glycerophospholipid biosynthetic process |
KEGG Pathway Links
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR004299 | Membrane bound O-acyl transferase, MBOAT |
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Biophysicochemical Properties | Kinetic parameters: KM=10 uM for oleoyl-CoA (with lysophosphoethanolamine as cosubstrate); KM=17 uM for palmitoleoyl-CoA (with lysophosphoethanolamine as cosubstrate); KM=0.9 uM for palmitoyl-CoA (with lysophosphoethanolamine as cosubstrate); KM=0.4 uM for myristoyl-CoA (with lysophosphoethanolamine as cosubstrate); KM=49 uM for oleoyl-CoA (with lysophosphocholine as cosubstrate); KM=21 uM for palmitoleoyl-CoA (with lysophosphocholine as cosubstrate); KM=1.8 uM for palmitoyl-CoA (with lysophosphocholine as cosubstrate); KM=5.9 uM for stearoyl-CoA (with lysophosphocholine as cosubstrate); KM=11 uM for arachidonyl-CoA (with lysophosphocholine as cosubstrate); Vmax=38 nmol/min/mg enzyme with oleoyl-CoA as substrate (with lysophosphoethanolamine as cosubstrate); Vmax=44 nmol/min/mg enzyme with palmitoleoyl-CoA as substrate (with lysophosphoethanolamine as substrate); Vmax=3.7 nmol/min/mg enzyme with palmitoyl-CoA as substrate (with lysophosphoethanolamine as substrate); Vmax=1.2 nmol/min/mg enzyme with myristoyl-CoA as substrate (with lysophosphoethanolamine as substrate); Vmax=125 nmol/min/mg enzyme with oleoyl-CoA as substrate (with lysophosphocholine as substrate); Vmax=142 nmol/min/mg enzyme with palmitoleoyl-CoA as substrate (with lysophosphocholine as substrate); Vmax=7.8 nmol/min/mg enzyme with palmitoyl-CoA as substrate (with lysophosphocholine as substrate); Vmax=12 nmol/min/mg enzyme with stearoyl-CoA as substrate (with lysophosphocholine as substrate); Vmax=58 nmol/min/mg enzyme with arachidonyl-CoA as substrate (with lysophosphocholine as substrate); pH dependence: Optimum pH is 6.5-7.5.; |
| Catalytic Activity | Acyl-CoA + 1-acyl-sn-glycero-3- phosphoethanolamine = CoA + 1,2-diacyl-sn-glycero-3- phosphoethanolamine. |
| Catalytic Activity | Acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine = CoA + 1,2-diacyl-sn-glycero-3-phosphocholine. |
| Catalytic Activity | Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate. |
| Function | Membrane-bound O-acyltransferase that mediates the incorporation of unsaturated acyl chains into the sn-2 position of phospholipids. Preferentially acylates lysophosphocholine, but also lysophosphoethanolamine and lysophosphatidylglycerol. {ECO:0000269|PubMed:17652094, ECO:0000269|PubMed:17675291, ECO:0000269|PubMed:17726007, ECO:0000269|PubMed:17996202}. |
| Similarity | Belongs to the membrane-bound acyltransferase family. {ECO:0000305}. |
| Subcellular Location | Endoplasmic reticulum membrane; Multi-pass membrane protein. Microsome membrane; Multi-pass membrane protein. |
Identical and Related Proteins
Unique RefSeq proteins for LMP007254 (as displayed in Record Overview)
Identical Sequences to LMP007254 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:6324749 | EMBL | CDL78926.1 | 619 | unnamed protein product [Saccharomyces cerevisiae] |
| GI:6324749 | GenBank | EWG83269.1 | 619 | Ale1p [Saccharomyces cerevisiae R008] |
| GI:6324749 | GenBank | EWG88479.1 | 619 | Ale1p [Saccharomyces cerevisiae P301] |
| GI:6324749 | GenBank | EWG93602.1 | 619 | Ale1p [Saccharomyces cerevisiae R103] |
| GI:6324749 | GenBank | EWH15997.1 | 619 | Ale1p [Saccharomyces cerevisiae P283] |
| GI:6324749 | gnl | McCuskerlabDuke | 619 | Ale1p [Saccharomyces cerevisiae YJM993] |
Related Sequences to LMP007254 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:6324749 | GenBank | EDN64033.1 | 619 | acyltransferase for lyso-phosphatidylethanolamine [Saccharomyces cerevisiae YJM789] |
| GI:6324749 | GenBank | EDV10758.1 | 616 | hypothetical protein SCRG_01566 [Saccharomyces cerevisiae RM11-1a] |
| GI:6324749 | GenBank | EEU07750.1 | 619 | Ale1p [Saccharomyces cerevisiae JAY291] |
| GI:6324749 | GenBank | EHN00131.1 | 620 | Ale1p [Saccharomyces cerevisiae x Saccharomyces kudriavzevii VIN7] |
| GI:6324749 | GenBank | EJS41668.1 | 619 | YOR175C [Saccharomyces arboricola H-6] |
| GI:6324749 | GenBank | EJT43770.1 | 619 | ALE1-like protein [Saccharomyces kudriavzevii IFO 1802] |